Job ID = 14160387 SRX = SRX8151886 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 20838966 spots for SRR11584264/SRR11584264.sra Written 20838966 spots for SRR11584264/SRR11584264.sra fastq に変換しました。 bowtie でマッピング中... Your job 14160516 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:26 20838966 reads; of these: 20838966 (100.00%) were unpaired; of these: 871585 (4.18%) aligned 0 times 16564311 (79.49%) aligned exactly 1 time 3403070 (16.33%) aligned >1 times 95.82% overall alignment rate Time searching: 00:04:26 Overall time: 00:04:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 6359141 / 19967381 = 0.3185 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 02:12:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8151886/SRX8151886.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8151886/SRX8151886.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8151886/SRX8151886.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8151886/SRX8151886.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 02:12:10: #1 read tag files... INFO @ Thu, 09 Dec 2021 02:12:10: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 02:12:14: 1000000 INFO @ Thu, 09 Dec 2021 02:12:19: 2000000 INFO @ Thu, 09 Dec 2021 02:12:23: 3000000 INFO @ Thu, 09 Dec 2021 02:12:28: 4000000 INFO @ Thu, 09 Dec 2021 02:12:32: 5000000 INFO @ Thu, 09 Dec 2021 02:12:36: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 02:12:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8151886/SRX8151886.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8151886/SRX8151886.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8151886/SRX8151886.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8151886/SRX8151886.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 02:12:40: #1 read tag files... INFO @ Thu, 09 Dec 2021 02:12:40: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 02:12:41: 7000000 INFO @ Thu, 09 Dec 2021 02:12:44: 1000000 INFO @ Thu, 09 Dec 2021 02:12:45: 8000000 INFO @ Thu, 09 Dec 2021 02:12:49: 2000000 INFO @ Thu, 09 Dec 2021 02:12:50: 9000000 INFO @ Thu, 09 Dec 2021 02:12:53: 3000000 INFO @ Thu, 09 Dec 2021 02:12:54: 10000000 INFO @ Thu, 09 Dec 2021 02:12:58: 4000000 INFO @ Thu, 09 Dec 2021 02:12:59: 11000000 INFO @ Thu, 09 Dec 2021 02:13:02: 5000000 INFO @ Thu, 09 Dec 2021 02:13:03: 12000000 INFO @ Thu, 09 Dec 2021 02:13:07: 6000000 INFO @ Thu, 09 Dec 2021 02:13:08: 13000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 02:13:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8151886/SRX8151886.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8151886/SRX8151886.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8151886/SRX8151886.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8151886/SRX8151886.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 02:13:10: #1 read tag files... INFO @ Thu, 09 Dec 2021 02:13:10: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 02:13:10: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 02:13:10: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 02:13:10: #1 total tags in treatment: 13608240 INFO @ Thu, 09 Dec 2021 02:13:10: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 02:13:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 02:13:11: #1 tags after filtering in treatment: 13608240 INFO @ Thu, 09 Dec 2021 02:13:11: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 02:13:11: #1 finished! INFO @ Thu, 09 Dec 2021 02:13:11: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 02:13:11: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 02:13:11: 7000000 INFO @ Thu, 09 Dec 2021 02:13:12: #2 number of paired peaks: 3372 INFO @ Thu, 09 Dec 2021 02:13:12: start model_add_line... INFO @ Thu, 09 Dec 2021 02:13:12: start X-correlation... INFO @ Thu, 09 Dec 2021 02:13:12: end of X-cor INFO @ Thu, 09 Dec 2021 02:13:12: #2 finished! INFO @ Thu, 09 Dec 2021 02:13:12: #2 predicted fragment length is 268 bps INFO @ Thu, 09 Dec 2021 02:13:12: #2 alternative fragment length(s) may be 268 bps INFO @ Thu, 09 Dec 2021 02:13:12: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX8151886/SRX8151886.05_model.r INFO @ Thu, 09 Dec 2021 02:13:12: #3 Call peaks... INFO @ Thu, 09 Dec 2021 02:13:12: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 02:13:14: 1000000 INFO @ Thu, 09 Dec 2021 02:13:16: 8000000 INFO @ Thu, 09 Dec 2021 02:13:19: 2000000 INFO @ Thu, 09 Dec 2021 02:13:20: 9000000 INFO @ Thu, 09 Dec 2021 02:13:23: 3000000 INFO @ Thu, 09 Dec 2021 02:13:25: 10000000 INFO @ Thu, 09 Dec 2021 02:13:28: 4000000 INFO @ Thu, 09 Dec 2021 02:13:29: 11000000 INFO @ Thu, 09 Dec 2021 02:13:32: 5000000 INFO @ Thu, 09 Dec 2021 02:13:34: 12000000 INFO @ Thu, 09 Dec 2021 02:13:36: 6000000 INFO @ Thu, 09 Dec 2021 02:13:38: 13000000 INFO @ Thu, 09 Dec 2021 02:13:41: 7000000 INFO @ Thu, 09 Dec 2021 02:13:41: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 02:13:41: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 02:13:41: #1 total tags in treatment: 13608240 INFO @ Thu, 09 Dec 2021 02:13:41: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 02:13:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 02:13:41: #1 tags after filtering in treatment: 13608240 INFO @ Thu, 09 Dec 2021 02:13:41: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 02:13:41: #1 finished! INFO @ Thu, 09 Dec 2021 02:13:41: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 02:13:41: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 02:13:42: #2 number of paired peaks: 3372 INFO @ Thu, 09 Dec 2021 02:13:42: start model_add_line... INFO @ Thu, 09 Dec 2021 02:13:42: start X-correlation... INFO @ Thu, 09 Dec 2021 02:13:43: end of X-cor INFO @ Thu, 09 Dec 2021 02:13:43: #2 finished! INFO @ Thu, 09 Dec 2021 02:13:43: #2 predicted fragment length is 268 bps INFO @ Thu, 09 Dec 2021 02:13:43: #2 alternative fragment length(s) may be 268 bps INFO @ Thu, 09 Dec 2021 02:13:43: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX8151886/SRX8151886.10_model.r INFO @ Thu, 09 Dec 2021 02:13:43: #3 Call peaks... INFO @ Thu, 09 Dec 2021 02:13:43: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 02:13:45: 8000000 INFO @ Thu, 09 Dec 2021 02:13:50: 9000000 INFO @ Thu, 09 Dec 2021 02:13:54: 10000000 INFO @ Thu, 09 Dec 2021 02:13:55: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 02:13:59: 11000000 INFO @ Thu, 09 Dec 2021 02:14:03: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 09 Dec 2021 02:14:07: 13000000 INFO @ Thu, 09 Dec 2021 02:14:10: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 02:14:10: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 02:14:10: #1 total tags in treatment: 13608240 INFO @ Thu, 09 Dec 2021 02:14:10: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 02:14:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 02:14:10: #1 tags after filtering in treatment: 13608240 INFO @ Thu, 09 Dec 2021 02:14:10: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 02:14:10: #1 finished! INFO @ Thu, 09 Dec 2021 02:14:10: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 02:14:10: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 02:14:11: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX8151886/SRX8151886.05_peaks.xls INFO @ Thu, 09 Dec 2021 02:14:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX8151886/SRX8151886.05_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 02:14:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX8151886/SRX8151886.05_summits.bed INFO @ Thu, 09 Dec 2021 02:14:11: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (10954 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 02:14:12: #2 number of paired peaks: 3372 INFO @ Thu, 09 Dec 2021 02:14:12: start model_add_line... INFO @ Thu, 09 Dec 2021 02:14:12: start X-correlation... INFO @ Thu, 09 Dec 2021 02:14:12: end of X-cor INFO @ Thu, 09 Dec 2021 02:14:12: #2 finished! INFO @ Thu, 09 Dec 2021 02:14:12: #2 predicted fragment length is 268 bps INFO @ Thu, 09 Dec 2021 02:14:12: #2 alternative fragment length(s) may be 268 bps INFO @ Thu, 09 Dec 2021 02:14:12: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX8151886/SRX8151886.20_model.r INFO @ Thu, 09 Dec 2021 02:14:12: #3 Call peaks... INFO @ Thu, 09 Dec 2021 02:14:12: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 02:14:25: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Thu, 09 Dec 2021 02:14:41: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX8151886/SRX8151886.10_peaks.xls INFO @ Thu, 09 Dec 2021 02:14:41: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX8151886/SRX8151886.10_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 02:14:41: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX8151886/SRX8151886.10_summits.bed INFO @ Thu, 09 Dec 2021 02:14:41: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (8618 records, 4 fields): 10 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 02:14:55: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 02:15:11: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX8151886/SRX8151886.20_peaks.xls INFO @ Thu, 09 Dec 2021 02:15:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX8151886/SRX8151886.20_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 02:15:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX8151886/SRX8151886.20_summits.bed INFO @ Thu, 09 Dec 2021 02:15:11: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (6146 records, 4 fields): 7 millis CompletedMACS2peakCalling