Job ID = 14160385 SRX = SRX8151884 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 43927762 spots for SRR11584262/SRR11584262.sra Written 43927762 spots for SRR11584262/SRR11584262.sra fastq に変換しました。 bowtie でマッピング中... Your job 14160525 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:58 43927762 reads; of these: 43927762 (100.00%) were unpaired; of these: 9010410 (20.51%) aligned 0 times 29721852 (67.66%) aligned exactly 1 time 5195500 (11.83%) aligned >1 times 79.49% overall alignment rate Time searching: 00:07:58 Overall time: 00:07:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 15438873 / 34917352 = 0.4422 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 02:21:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8151884/SRX8151884.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8151884/SRX8151884.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8151884/SRX8151884.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8151884/SRX8151884.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 02:21:17: #1 read tag files... INFO @ Thu, 09 Dec 2021 02:21:17: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 02:21:21: 1000000 INFO @ Thu, 09 Dec 2021 02:21:26: 2000000 INFO @ Thu, 09 Dec 2021 02:21:30: 3000000 INFO @ Thu, 09 Dec 2021 02:21:35: 4000000 INFO @ Thu, 09 Dec 2021 02:21:40: 5000000 INFO @ Thu, 09 Dec 2021 02:21:44: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 02:21:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8151884/SRX8151884.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8151884/SRX8151884.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8151884/SRX8151884.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8151884/SRX8151884.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 02:21:46: #1 read tag files... INFO @ Thu, 09 Dec 2021 02:21:46: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 02:21:49: 7000000 INFO @ Thu, 09 Dec 2021 02:21:52: 1000000 INFO @ Thu, 09 Dec 2021 02:21:54: 8000000 INFO @ Thu, 09 Dec 2021 02:21:58: 2000000 INFO @ Thu, 09 Dec 2021 02:21:59: 9000000 INFO @ Thu, 09 Dec 2021 02:22:03: 3000000 INFO @ Thu, 09 Dec 2021 02:22:04: 10000000 INFO @ Thu, 09 Dec 2021 02:22:09: 11000000 INFO @ Thu, 09 Dec 2021 02:22:09: 4000000 INFO @ Thu, 09 Dec 2021 02:22:14: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 02:22:15: 5000000 INFO @ Thu, 09 Dec 2021 02:22:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8151884/SRX8151884.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8151884/SRX8151884.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8151884/SRX8151884.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8151884/SRX8151884.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 02:22:16: #1 read tag files... INFO @ Thu, 09 Dec 2021 02:22:16: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 02:22:19: 13000000 INFO @ Thu, 09 Dec 2021 02:22:21: 6000000 INFO @ Thu, 09 Dec 2021 02:22:22: 1000000 INFO @ Thu, 09 Dec 2021 02:22:24: 14000000 INFO @ Thu, 09 Dec 2021 02:22:26: 7000000 INFO @ Thu, 09 Dec 2021 02:22:28: 2000000 INFO @ Thu, 09 Dec 2021 02:22:29: 15000000 INFO @ Thu, 09 Dec 2021 02:22:32: 8000000 INFO @ Thu, 09 Dec 2021 02:22:34: 3000000 INFO @ Thu, 09 Dec 2021 02:22:34: 16000000 INFO @ Thu, 09 Dec 2021 02:22:38: 9000000 INFO @ Thu, 09 Dec 2021 02:22:39: 17000000 INFO @ Thu, 09 Dec 2021 02:22:39: 4000000 INFO @ Thu, 09 Dec 2021 02:22:43: 10000000 INFO @ Thu, 09 Dec 2021 02:22:44: 18000000 INFO @ Thu, 09 Dec 2021 02:22:45: 5000000 INFO @ Thu, 09 Dec 2021 02:22:49: 19000000 INFO @ Thu, 09 Dec 2021 02:22:49: 11000000 INFO @ Thu, 09 Dec 2021 02:22:51: 6000000 INFO @ Thu, 09 Dec 2021 02:22:51: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 02:22:51: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 02:22:51: #1 total tags in treatment: 19478479 INFO @ Thu, 09 Dec 2021 02:22:51: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 02:22:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 02:22:52: #1 tags after filtering in treatment: 19478479 INFO @ Thu, 09 Dec 2021 02:22:52: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 02:22:52: #1 finished! INFO @ Thu, 09 Dec 2021 02:22:52: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 02:22:52: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 02:22:53: #2 number of paired peaks: 3041 INFO @ Thu, 09 Dec 2021 02:22:53: start model_add_line... INFO @ Thu, 09 Dec 2021 02:22:53: start X-correlation... INFO @ Thu, 09 Dec 2021 02:22:53: end of X-cor INFO @ Thu, 09 Dec 2021 02:22:53: #2 finished! INFO @ Thu, 09 Dec 2021 02:22:53: #2 predicted fragment length is 250 bps INFO @ Thu, 09 Dec 2021 02:22:53: #2 alternative fragment length(s) may be 4,250 bps INFO @ Thu, 09 Dec 2021 02:22:53: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX8151884/SRX8151884.05_model.r INFO @ Thu, 09 Dec 2021 02:22:53: #3 Call peaks... INFO @ Thu, 09 Dec 2021 02:22:53: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 02:22:55: 12000000 INFO @ Thu, 09 Dec 2021 02:22:57: 7000000 INFO @ Thu, 09 Dec 2021 02:23:01: 13000000 INFO @ Thu, 09 Dec 2021 02:23:02: 8000000 INFO @ Thu, 09 Dec 2021 02:23:06: 14000000 INFO @ Thu, 09 Dec 2021 02:23:08: 9000000 INFO @ Thu, 09 Dec 2021 02:23:12: 15000000 INFO @ Thu, 09 Dec 2021 02:23:14: 10000000 INFO @ Thu, 09 Dec 2021 02:23:18: 16000000 INFO @ Thu, 09 Dec 2021 02:23:20: 11000000 INFO @ Thu, 09 Dec 2021 02:23:23: 17000000 INFO @ Thu, 09 Dec 2021 02:23:25: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 09 Dec 2021 02:23:29: 18000000 INFO @ Thu, 09 Dec 2021 02:23:31: 13000000 INFO @ Thu, 09 Dec 2021 02:23:35: 19000000 INFO @ Thu, 09 Dec 2021 02:23:37: 14000000 INFO @ Thu, 09 Dec 2021 02:23:38: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 02:23:38: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 02:23:38: #1 total tags in treatment: 19478479 INFO @ Thu, 09 Dec 2021 02:23:38: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 02:23:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 02:23:38: #1 tags after filtering in treatment: 19478479 INFO @ Thu, 09 Dec 2021 02:23:38: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 02:23:38: #1 finished! INFO @ Thu, 09 Dec 2021 02:23:38: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 02:23:38: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 02:23:39: #2 number of paired peaks: 3041 INFO @ Thu, 09 Dec 2021 02:23:39: start model_add_line... INFO @ Thu, 09 Dec 2021 02:23:40: start X-correlation... INFO @ Thu, 09 Dec 2021 02:23:40: end of X-cor INFO @ Thu, 09 Dec 2021 02:23:40: #2 finished! INFO @ Thu, 09 Dec 2021 02:23:40: #2 predicted fragment length is 250 bps INFO @ Thu, 09 Dec 2021 02:23:40: #2 alternative fragment length(s) may be 4,250 bps INFO @ Thu, 09 Dec 2021 02:23:40: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX8151884/SRX8151884.10_model.r INFO @ Thu, 09 Dec 2021 02:23:40: #3 Call peaks... INFO @ Thu, 09 Dec 2021 02:23:40: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 02:23:43: 15000000 INFO @ Thu, 09 Dec 2021 02:23:48: 16000000 INFO @ Thu, 09 Dec 2021 02:23:50: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 02:23:54: 17000000 INFO @ Thu, 09 Dec 2021 02:23:59: 18000000 BigWig に変換しました。 INFO @ Thu, 09 Dec 2021 02:24:05: 19000000 INFO @ Thu, 09 Dec 2021 02:24:08: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 02:24:08: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 02:24:08: #1 total tags in treatment: 19478479 INFO @ Thu, 09 Dec 2021 02:24:08: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 02:24:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 02:24:08: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX8151884/SRX8151884.05_peaks.xls INFO @ Thu, 09 Dec 2021 02:24:08: #1 tags after filtering in treatment: 19478479 INFO @ Thu, 09 Dec 2021 02:24:08: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 02:24:08: #1 finished! INFO @ Thu, 09 Dec 2021 02:24:08: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 02:24:08: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 02:24:08: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX8151884/SRX8151884.05_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 02:24:08: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX8151884/SRX8151884.05_summits.bed INFO @ Thu, 09 Dec 2021 02:24:08: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (14497 records, 4 fields): 15 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 02:24:10: #2 number of paired peaks: 3041 INFO @ Thu, 09 Dec 2021 02:24:10: start model_add_line... INFO @ Thu, 09 Dec 2021 02:24:10: start X-correlation... INFO @ Thu, 09 Dec 2021 02:24:10: end of X-cor INFO @ Thu, 09 Dec 2021 02:24:10: #2 finished! INFO @ Thu, 09 Dec 2021 02:24:10: #2 predicted fragment length is 250 bps INFO @ Thu, 09 Dec 2021 02:24:10: #2 alternative fragment length(s) may be 4,250 bps INFO @ Thu, 09 Dec 2021 02:24:10: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX8151884/SRX8151884.20_model.r INFO @ Thu, 09 Dec 2021 02:24:10: #3 Call peaks... INFO @ Thu, 09 Dec 2021 02:24:10: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 02:24:37: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 02:24:54: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX8151884/SRX8151884.10_peaks.xls INFO @ Thu, 09 Dec 2021 02:24:55: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX8151884/SRX8151884.10_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 02:24:55: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX8151884/SRX8151884.10_summits.bed INFO @ Thu, 09 Dec 2021 02:24:55: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (11949 records, 4 fields): 12 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 02:25:06: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 02:25:23: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX8151884/SRX8151884.20_peaks.xls INFO @ Thu, 09 Dec 2021 02:25:23: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX8151884/SRX8151884.20_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 02:25:23: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX8151884/SRX8151884.20_summits.bed INFO @ Thu, 09 Dec 2021 02:25:23: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (9051 records, 4 fields): 10 millis CompletedMACS2peakCalling