Job ID = 14160326 SRX = SRX8151844 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 30332501 spots for SRR11584261/SRR11584261.sra Written 30332501 spots for SRR11584261/SRR11584261.sra fastq に変換しました。 bowtie でマッピング中... Your job 14160448 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:58 30332501 reads; of these: 30332501 (100.00%) were unpaired; of these: 4378862 (14.44%) aligned 0 times 20765911 (68.46%) aligned exactly 1 time 5187728 (17.10%) aligned >1 times 85.56% overall alignment rate Time searching: 00:05:58 Overall time: 00:05:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 9421776 / 25953639 = 0.3630 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 01:38:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8151844/SRX8151844.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8151844/SRX8151844.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8151844/SRX8151844.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8151844/SRX8151844.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 01:38:48: #1 read tag files... INFO @ Thu, 09 Dec 2021 01:38:48: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 01:38:54: 1000000 INFO @ Thu, 09 Dec 2021 01:38:59: 2000000 INFO @ Thu, 09 Dec 2021 01:39:05: 3000000 INFO @ Thu, 09 Dec 2021 01:39:10: 4000000 INFO @ Thu, 09 Dec 2021 01:39:16: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 01:39:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8151844/SRX8151844.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8151844/SRX8151844.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8151844/SRX8151844.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8151844/SRX8151844.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 01:39:19: #1 read tag files... INFO @ Thu, 09 Dec 2021 01:39:19: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 01:39:21: 6000000 INFO @ Thu, 09 Dec 2021 01:39:23: 1000000 INFO @ Thu, 09 Dec 2021 01:39:27: 7000000 INFO @ Thu, 09 Dec 2021 01:39:28: 2000000 INFO @ Thu, 09 Dec 2021 01:39:33: 3000000 INFO @ Thu, 09 Dec 2021 01:39:33: 8000000 INFO @ Thu, 09 Dec 2021 01:39:37: 4000000 INFO @ Thu, 09 Dec 2021 01:39:38: 9000000 INFO @ Thu, 09 Dec 2021 01:39:42: 5000000 INFO @ Thu, 09 Dec 2021 01:39:44: 10000000 INFO @ Thu, 09 Dec 2021 01:39:47: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 01:39:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8151844/SRX8151844.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8151844/SRX8151844.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8151844/SRX8151844.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8151844/SRX8151844.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 01:39:49: #1 read tag files... INFO @ Thu, 09 Dec 2021 01:39:49: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 01:39:49: 11000000 INFO @ Thu, 09 Dec 2021 01:39:51: 7000000 INFO @ Thu, 09 Dec 2021 01:39:54: 1000000 INFO @ Thu, 09 Dec 2021 01:39:55: 12000000 INFO @ Thu, 09 Dec 2021 01:39:56: 8000000 INFO @ Thu, 09 Dec 2021 01:40:00: 2000000 INFO @ Thu, 09 Dec 2021 01:40:01: 9000000 INFO @ Thu, 09 Dec 2021 01:40:01: 13000000 INFO @ Thu, 09 Dec 2021 01:40:05: 3000000 INFO @ Thu, 09 Dec 2021 01:40:05: 10000000 INFO @ Thu, 09 Dec 2021 01:40:06: 14000000 INFO @ Thu, 09 Dec 2021 01:40:10: 11000000 INFO @ Thu, 09 Dec 2021 01:40:10: 4000000 INFO @ Thu, 09 Dec 2021 01:40:12: 15000000 INFO @ Thu, 09 Dec 2021 01:40:15: 12000000 INFO @ Thu, 09 Dec 2021 01:40:16: 5000000 INFO @ Thu, 09 Dec 2021 01:40:18: 16000000 INFO @ Thu, 09 Dec 2021 01:40:20: 13000000 INFO @ Thu, 09 Dec 2021 01:40:21: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 01:40:21: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 01:40:21: #1 total tags in treatment: 16531863 INFO @ Thu, 09 Dec 2021 01:40:21: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 01:40:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 01:40:21: #1 tags after filtering in treatment: 16531863 INFO @ Thu, 09 Dec 2021 01:40:21: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 01:40:21: #1 finished! INFO @ Thu, 09 Dec 2021 01:40:21: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 01:40:21: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 01:40:21: 6000000 INFO @ Thu, 09 Dec 2021 01:40:23: #2 number of paired peaks: 3419 INFO @ Thu, 09 Dec 2021 01:40:23: start model_add_line... INFO @ Thu, 09 Dec 2021 01:40:23: start X-correlation... INFO @ Thu, 09 Dec 2021 01:40:23: end of X-cor INFO @ Thu, 09 Dec 2021 01:40:23: #2 finished! INFO @ Thu, 09 Dec 2021 01:40:23: #2 predicted fragment length is 201 bps INFO @ Thu, 09 Dec 2021 01:40:23: #2 alternative fragment length(s) may be 201 bps INFO @ Thu, 09 Dec 2021 01:40:23: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX8151844/SRX8151844.05_model.r INFO @ Thu, 09 Dec 2021 01:40:23: #3 Call peaks... INFO @ Thu, 09 Dec 2021 01:40:23: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 01:40:24: 14000000 INFO @ Thu, 09 Dec 2021 01:40:27: 7000000 INFO @ Thu, 09 Dec 2021 01:40:29: 15000000 INFO @ Thu, 09 Dec 2021 01:40:32: 8000000 INFO @ Thu, 09 Dec 2021 01:40:34: 16000000 INFO @ Thu, 09 Dec 2021 01:40:36: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 01:40:36: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 01:40:36: #1 total tags in treatment: 16531863 INFO @ Thu, 09 Dec 2021 01:40:36: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 01:40:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 01:40:37: #1 tags after filtering in treatment: 16531863 INFO @ Thu, 09 Dec 2021 01:40:37: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 01:40:37: #1 finished! INFO @ Thu, 09 Dec 2021 01:40:37: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 01:40:37: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 01:40:38: 9000000 INFO @ Thu, 09 Dec 2021 01:40:38: #2 number of paired peaks: 3419 INFO @ Thu, 09 Dec 2021 01:40:38: start model_add_line... INFO @ Thu, 09 Dec 2021 01:40:38: start X-correlation... INFO @ Thu, 09 Dec 2021 01:40:38: end of X-cor INFO @ Thu, 09 Dec 2021 01:40:38: #2 finished! INFO @ Thu, 09 Dec 2021 01:40:38: #2 predicted fragment length is 201 bps INFO @ Thu, 09 Dec 2021 01:40:38: #2 alternative fragment length(s) may be 201 bps INFO @ Thu, 09 Dec 2021 01:40:38: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX8151844/SRX8151844.10_model.r INFO @ Thu, 09 Dec 2021 01:40:38: #3 Call peaks... INFO @ Thu, 09 Dec 2021 01:40:38: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 01:40:43: 10000000 INFO @ Thu, 09 Dec 2021 01:40:48: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 09 Dec 2021 01:40:54: 12000000 INFO @ Thu, 09 Dec 2021 01:40:59: 13000000 INFO @ Thu, 09 Dec 2021 01:41:04: 14000000 INFO @ Thu, 09 Dec 2021 01:41:09: 15000000 INFO @ Thu, 09 Dec 2021 01:41:11: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 01:41:15: 16000000 INFO @ Thu, 09 Dec 2021 01:41:18: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 01:41:18: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 01:41:18: #1 total tags in treatment: 16531863 INFO @ Thu, 09 Dec 2021 01:41:18: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 01:41:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 01:41:18: #1 tags after filtering in treatment: 16531863 INFO @ Thu, 09 Dec 2021 01:41:18: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 01:41:18: #1 finished! INFO @ Thu, 09 Dec 2021 01:41:18: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 01:41:18: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Thu, 09 Dec 2021 01:41:19: #2 number of paired peaks: 3419 INFO @ Thu, 09 Dec 2021 01:41:19: start model_add_line... INFO @ Thu, 09 Dec 2021 01:41:20: start X-correlation... INFO @ Thu, 09 Dec 2021 01:41:20: end of X-cor INFO @ Thu, 09 Dec 2021 01:41:20: #2 finished! INFO @ Thu, 09 Dec 2021 01:41:20: #2 predicted fragment length is 201 bps INFO @ Thu, 09 Dec 2021 01:41:20: #2 alternative fragment length(s) may be 201 bps INFO @ Thu, 09 Dec 2021 01:41:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX8151844/SRX8151844.20_model.r INFO @ Thu, 09 Dec 2021 01:41:20: #3 Call peaks... INFO @ Thu, 09 Dec 2021 01:41:20: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 01:41:25: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 01:41:29: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX8151844/SRX8151844.05_peaks.xls INFO @ Thu, 09 Dec 2021 01:41:30: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX8151844/SRX8151844.05_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 01:41:30: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX8151844/SRX8151844.05_summits.bed INFO @ Thu, 09 Dec 2021 01:41:30: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (12827 records, 4 fields): 14 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 01:41:44: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX8151844/SRX8151844.10_peaks.xls INFO @ Thu, 09 Dec 2021 01:41:44: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX8151844/SRX8151844.10_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 01:41:44: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX8151844/SRX8151844.10_summits.bed INFO @ Thu, 09 Dec 2021 01:41:44: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (9948 records, 4 fields): 10 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 01:42:09: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 01:42:27: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX8151844/SRX8151844.20_peaks.xls INFO @ Thu, 09 Dec 2021 01:42:27: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX8151844/SRX8151844.20_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 01:42:27: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX8151844/SRX8151844.20_summits.bed INFO @ Thu, 09 Dec 2021 01:42:27: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (6835 records, 4 fields): 8 millis CompletedMACS2peakCalling