Job ID = 14160325 SRX = SRX8151843 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 37091956 spots for SRR11584260/SRR11584260.sra Written 37091956 spots for SRR11584260/SRR11584260.sra fastq に変換しました。 bowtie でマッピング中... Your job 14160450 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:19 37091956 reads; of these: 37091956 (100.00%) were unpaired; of these: 3714884 (10.02%) aligned 0 times 27559219 (74.30%) aligned exactly 1 time 5817853 (15.68%) aligned >1 times 89.98% overall alignment rate Time searching: 00:07:19 Overall time: 00:07:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 13283087 / 33377072 = 0.3980 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 01:41:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8151843/SRX8151843.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8151843/SRX8151843.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8151843/SRX8151843.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8151843/SRX8151843.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 01:41:30: #1 read tag files... INFO @ Thu, 09 Dec 2021 01:41:30: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 01:41:35: 1000000 INFO @ Thu, 09 Dec 2021 01:41:41: 2000000 INFO @ Thu, 09 Dec 2021 01:41:46: 3000000 INFO @ Thu, 09 Dec 2021 01:41:51: 4000000 INFO @ Thu, 09 Dec 2021 01:41:57: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 01:42:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8151843/SRX8151843.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8151843/SRX8151843.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8151843/SRX8151843.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8151843/SRX8151843.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 01:42:00: #1 read tag files... INFO @ Thu, 09 Dec 2021 01:42:00: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 01:42:02: 6000000 INFO @ Thu, 09 Dec 2021 01:42:05: 1000000 INFO @ Thu, 09 Dec 2021 01:42:08: 7000000 INFO @ Thu, 09 Dec 2021 01:42:09: 2000000 INFO @ Thu, 09 Dec 2021 01:42:13: 8000000 INFO @ Thu, 09 Dec 2021 01:42:14: 3000000 INFO @ Thu, 09 Dec 2021 01:42:18: 4000000 INFO @ Thu, 09 Dec 2021 01:42:18: 9000000 INFO @ Thu, 09 Dec 2021 01:42:23: 5000000 INFO @ Thu, 09 Dec 2021 01:42:24: 10000000 INFO @ Thu, 09 Dec 2021 01:42:27: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 01:42:29: 11000000 INFO @ Thu, 09 Dec 2021 01:42:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8151843/SRX8151843.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8151843/SRX8151843.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8151843/SRX8151843.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8151843/SRX8151843.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 01:42:30: #1 read tag files... INFO @ Thu, 09 Dec 2021 01:42:30: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 01:42:32: 7000000 INFO @ Thu, 09 Dec 2021 01:42:34: 12000000 INFO @ Thu, 09 Dec 2021 01:42:35: 1000000 INFO @ Thu, 09 Dec 2021 01:42:36: 8000000 INFO @ Thu, 09 Dec 2021 01:42:39: 2000000 INFO @ Thu, 09 Dec 2021 01:42:39: 13000000 INFO @ Thu, 09 Dec 2021 01:42:41: 9000000 INFO @ Thu, 09 Dec 2021 01:42:44: 3000000 INFO @ Thu, 09 Dec 2021 01:42:45: 14000000 INFO @ Thu, 09 Dec 2021 01:42:45: 10000000 INFO @ Thu, 09 Dec 2021 01:42:48: 4000000 INFO @ Thu, 09 Dec 2021 01:42:50: 11000000 INFO @ Thu, 09 Dec 2021 01:42:50: 15000000 INFO @ Thu, 09 Dec 2021 01:42:53: 5000000 INFO @ Thu, 09 Dec 2021 01:42:54: 12000000 INFO @ Thu, 09 Dec 2021 01:42:55: 16000000 INFO @ Thu, 09 Dec 2021 01:42:57: 6000000 INFO @ Thu, 09 Dec 2021 01:42:59: 13000000 INFO @ Thu, 09 Dec 2021 01:43:01: 17000000 INFO @ Thu, 09 Dec 2021 01:43:02: 7000000 INFO @ Thu, 09 Dec 2021 01:43:04: 14000000 INFO @ Thu, 09 Dec 2021 01:43:06: 18000000 INFO @ Thu, 09 Dec 2021 01:43:06: 8000000 INFO @ Thu, 09 Dec 2021 01:43:08: 15000000 INFO @ Thu, 09 Dec 2021 01:43:11: 9000000 INFO @ Thu, 09 Dec 2021 01:43:11: 19000000 INFO @ Thu, 09 Dec 2021 01:43:13: 16000000 INFO @ Thu, 09 Dec 2021 01:43:16: 10000000 INFO @ Thu, 09 Dec 2021 01:43:17: 20000000 INFO @ Thu, 09 Dec 2021 01:43:17: 17000000 INFO @ Thu, 09 Dec 2021 01:43:17: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 01:43:17: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 01:43:17: #1 total tags in treatment: 20093985 INFO @ Thu, 09 Dec 2021 01:43:17: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 01:43:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 01:43:18: #1 tags after filtering in treatment: 20093985 INFO @ Thu, 09 Dec 2021 01:43:18: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 01:43:18: #1 finished! INFO @ Thu, 09 Dec 2021 01:43:18: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 01:43:18: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 01:43:19: #2 number of paired peaks: 2999 INFO @ Thu, 09 Dec 2021 01:43:19: start model_add_line... INFO @ Thu, 09 Dec 2021 01:43:19: start X-correlation... INFO @ Thu, 09 Dec 2021 01:43:19: end of X-cor INFO @ Thu, 09 Dec 2021 01:43:19: #2 finished! INFO @ Thu, 09 Dec 2021 01:43:19: #2 predicted fragment length is 256 bps INFO @ Thu, 09 Dec 2021 01:43:19: #2 alternative fragment length(s) may be 4,256 bps INFO @ Thu, 09 Dec 2021 01:43:19: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX8151843/SRX8151843.05_model.r INFO @ Thu, 09 Dec 2021 01:43:19: #3 Call peaks... INFO @ Thu, 09 Dec 2021 01:43:19: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 01:43:20: 11000000 INFO @ Thu, 09 Dec 2021 01:43:22: 18000000 INFO @ Thu, 09 Dec 2021 01:43:25: 12000000 INFO @ Thu, 09 Dec 2021 01:43:26: 19000000 INFO @ Thu, 09 Dec 2021 01:43:29: 13000000 INFO @ Thu, 09 Dec 2021 01:43:31: 20000000 INFO @ Thu, 09 Dec 2021 01:43:31: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 01:43:31: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 01:43:31: #1 total tags in treatment: 20093985 INFO @ Thu, 09 Dec 2021 01:43:31: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 01:43:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 01:43:32: #1 tags after filtering in treatment: 20093985 INFO @ Thu, 09 Dec 2021 01:43:32: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 01:43:32: #1 finished! INFO @ Thu, 09 Dec 2021 01:43:32: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 01:43:32: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 01:43:33: #2 number of paired peaks: 2999 INFO @ Thu, 09 Dec 2021 01:43:33: start model_add_line... INFO @ Thu, 09 Dec 2021 01:43:33: start X-correlation... INFO @ Thu, 09 Dec 2021 01:43:33: end of X-cor INFO @ Thu, 09 Dec 2021 01:43:33: #2 finished! INFO @ Thu, 09 Dec 2021 01:43:33: #2 predicted fragment length is 256 bps INFO @ Thu, 09 Dec 2021 01:43:33: #2 alternative fragment length(s) may be 4,256 bps INFO @ Thu, 09 Dec 2021 01:43:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX8151843/SRX8151843.10_model.r INFO @ Thu, 09 Dec 2021 01:43:33: #3 Call peaks... INFO @ Thu, 09 Dec 2021 01:43:33: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 01:43:33: 14000000 INFO @ Thu, 09 Dec 2021 01:43:38: 15000000 INFO @ Thu, 09 Dec 2021 01:43:42: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 09 Dec 2021 01:43:47: 17000000 INFO @ Thu, 09 Dec 2021 01:43:51: 18000000 INFO @ Thu, 09 Dec 2021 01:43:56: 19000000 INFO @ Thu, 09 Dec 2021 01:44:00: 20000000 INFO @ Thu, 09 Dec 2021 01:44:01: #1 tag size is determined as 50 bps INFO @ Thu, 09 Dec 2021 01:44:01: #1 tag size = 50 INFO @ Thu, 09 Dec 2021 01:44:01: #1 total tags in treatment: 20093985 INFO @ Thu, 09 Dec 2021 01:44:01: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 01:44:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 01:44:01: #1 tags after filtering in treatment: 20093985 INFO @ Thu, 09 Dec 2021 01:44:01: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 01:44:01: #1 finished! INFO @ Thu, 09 Dec 2021 01:44:01: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 01:44:01: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 01:44:03: #2 number of paired peaks: 2999 INFO @ Thu, 09 Dec 2021 01:44:03: start model_add_line... INFO @ Thu, 09 Dec 2021 01:44:03: start X-correlation... INFO @ Thu, 09 Dec 2021 01:44:03: end of X-cor INFO @ Thu, 09 Dec 2021 01:44:03: #2 finished! INFO @ Thu, 09 Dec 2021 01:44:03: #2 predicted fragment length is 256 bps INFO @ Thu, 09 Dec 2021 01:44:03: #2 alternative fragment length(s) may be 4,256 bps INFO @ Thu, 09 Dec 2021 01:44:03: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX8151843/SRX8151843.20_model.r INFO @ Thu, 09 Dec 2021 01:44:03: #3 Call peaks... INFO @ Thu, 09 Dec 2021 01:44:03: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Thu, 09 Dec 2021 01:44:20: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 01:44:35: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 01:44:40: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX8151843/SRX8151843.05_peaks.xls INFO @ Thu, 09 Dec 2021 01:44:40: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX8151843/SRX8151843.05_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 01:44:40: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX8151843/SRX8151843.05_summits.bed INFO @ Thu, 09 Dec 2021 01:44:40: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (13334 records, 4 fields): 14 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 01:44:55: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX8151843/SRX8151843.10_peaks.xls INFO @ Thu, 09 Dec 2021 01:44:55: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX8151843/SRX8151843.10_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 01:44:55: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX8151843/SRX8151843.10_summits.bed INFO @ Thu, 09 Dec 2021 01:44:55: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (10762 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 01:45:04: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 01:45:24: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX8151843/SRX8151843.20_peaks.xls INFO @ Thu, 09 Dec 2021 01:45:24: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX8151843/SRX8151843.20_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 01:45:24: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX8151843/SRX8151843.20_summits.bed INFO @ Thu, 09 Dec 2021 01:45:24: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (7849 records, 4 fields): 8 millis CompletedMACS2peakCalling