Job ID = 5720350 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 51,944,210 reads read : 51,944,210 reads written : 51,944,210 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:32 51944210 reads; of these: 51944210 (100.00%) were unpaired; of these: 1390111 (2.68%) aligned 0 times 45432397 (87.46%) aligned exactly 1 time 5121702 (9.86%) aligned >1 times 97.32% overall alignment rate Time searching: 00:16:32 Overall time: 00:16:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 14926042 / 50554099 = 0.2952 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:52:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7971810/SRX7971810.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7971810/SRX7971810.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7971810/SRX7971810.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7971810/SRX7971810.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:52:17: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:52:17: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:52:23: 1000000 INFO @ Thu, 16 Apr 2020 00:52:29: 2000000 INFO @ Thu, 16 Apr 2020 00:52:35: 3000000 INFO @ Thu, 16 Apr 2020 00:52:42: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:52:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7971810/SRX7971810.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7971810/SRX7971810.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7971810/SRX7971810.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7971810/SRX7971810.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:52:47: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:52:47: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:52:48: 5000000 INFO @ Thu, 16 Apr 2020 00:52:53: 1000000 INFO @ Thu, 16 Apr 2020 00:52:54: 6000000 INFO @ Thu, 16 Apr 2020 00:52:59: 2000000 INFO @ Thu, 16 Apr 2020 00:53:01: 7000000 INFO @ Thu, 16 Apr 2020 00:53:06: 3000000 INFO @ Thu, 16 Apr 2020 00:53:08: 8000000 INFO @ Thu, 16 Apr 2020 00:53:12: 4000000 INFO @ Thu, 16 Apr 2020 00:53:14: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:53:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7971810/SRX7971810.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7971810/SRX7971810.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7971810/SRX7971810.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7971810/SRX7971810.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:53:17: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:53:17: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:53:19: 5000000 INFO @ Thu, 16 Apr 2020 00:53:21: 10000000 INFO @ Thu, 16 Apr 2020 00:53:23: 1000000 INFO @ Thu, 16 Apr 2020 00:53:26: 6000000 INFO @ Thu, 16 Apr 2020 00:53:27: 11000000 INFO @ Thu, 16 Apr 2020 00:53:30: 2000000 INFO @ Thu, 16 Apr 2020 00:53:32: 7000000 INFO @ Thu, 16 Apr 2020 00:53:34: 12000000 INFO @ Thu, 16 Apr 2020 00:53:37: 3000000 INFO @ Thu, 16 Apr 2020 00:53:39: 8000000 INFO @ Thu, 16 Apr 2020 00:53:41: 13000000 INFO @ Thu, 16 Apr 2020 00:53:44: 4000000 INFO @ Thu, 16 Apr 2020 00:53:46: 9000000 INFO @ Thu, 16 Apr 2020 00:53:48: 14000000 INFO @ Thu, 16 Apr 2020 00:53:51: 5000000 INFO @ Thu, 16 Apr 2020 00:53:53: 10000000 INFO @ Thu, 16 Apr 2020 00:53:55: 15000000 INFO @ Thu, 16 Apr 2020 00:53:58: 6000000 INFO @ Thu, 16 Apr 2020 00:54:00: 11000000 INFO @ Thu, 16 Apr 2020 00:54:02: 16000000 INFO @ Thu, 16 Apr 2020 00:54:05: 7000000 INFO @ Thu, 16 Apr 2020 00:54:07: 12000000 INFO @ Thu, 16 Apr 2020 00:54:08: 17000000 INFO @ Thu, 16 Apr 2020 00:54:12: 8000000 INFO @ Thu, 16 Apr 2020 00:54:14: 13000000 INFO @ Thu, 16 Apr 2020 00:54:15: 18000000 INFO @ Thu, 16 Apr 2020 00:54:18: 9000000 INFO @ Thu, 16 Apr 2020 00:54:21: 14000000 INFO @ Thu, 16 Apr 2020 00:54:21: 19000000 INFO @ Thu, 16 Apr 2020 00:54:25: 10000000 INFO @ Thu, 16 Apr 2020 00:54:28: 20000000 INFO @ Thu, 16 Apr 2020 00:54:28: 15000000 INFO @ Thu, 16 Apr 2020 00:54:32: 11000000 INFO @ Thu, 16 Apr 2020 00:54:34: 21000000 INFO @ Thu, 16 Apr 2020 00:54:35: 16000000 INFO @ Thu, 16 Apr 2020 00:54:39: 12000000 INFO @ Thu, 16 Apr 2020 00:54:41: 22000000 INFO @ Thu, 16 Apr 2020 00:54:42: 17000000 INFO @ Thu, 16 Apr 2020 00:54:46: 13000000 INFO @ Thu, 16 Apr 2020 00:54:48: 23000000 INFO @ Thu, 16 Apr 2020 00:54:49: 18000000 INFO @ Thu, 16 Apr 2020 00:54:53: 14000000 INFO @ Thu, 16 Apr 2020 00:54:55: 24000000 INFO @ Thu, 16 Apr 2020 00:54:55: 19000000 INFO @ Thu, 16 Apr 2020 00:55:00: 15000000 INFO @ Thu, 16 Apr 2020 00:55:01: 25000000 INFO @ Thu, 16 Apr 2020 00:55:02: 20000000 INFO @ Thu, 16 Apr 2020 00:55:07: 16000000 INFO @ Thu, 16 Apr 2020 00:55:08: 26000000 INFO @ Thu, 16 Apr 2020 00:55:09: 21000000 INFO @ Thu, 16 Apr 2020 00:55:13: 17000000 INFO @ Thu, 16 Apr 2020 00:55:15: 27000000 INFO @ Thu, 16 Apr 2020 00:55:15: 22000000 INFO @ Thu, 16 Apr 2020 00:55:20: 18000000 INFO @ Thu, 16 Apr 2020 00:55:22: 23000000 INFO @ Thu, 16 Apr 2020 00:55:22: 28000000 INFO @ Thu, 16 Apr 2020 00:55:26: 19000000 INFO @ Thu, 16 Apr 2020 00:55:28: 24000000 INFO @ Thu, 16 Apr 2020 00:55:29: 29000000 INFO @ Thu, 16 Apr 2020 00:55:33: 20000000 INFO @ Thu, 16 Apr 2020 00:55:35: 25000000 INFO @ Thu, 16 Apr 2020 00:55:36: 30000000 INFO @ Thu, 16 Apr 2020 00:55:40: 21000000 INFO @ Thu, 16 Apr 2020 00:55:41: 26000000 INFO @ Thu, 16 Apr 2020 00:55:43: 31000000 INFO @ Thu, 16 Apr 2020 00:55:46: 22000000 INFO @ Thu, 16 Apr 2020 00:55:48: 27000000 INFO @ Thu, 16 Apr 2020 00:55:50: 32000000 INFO @ Thu, 16 Apr 2020 00:55:53: 23000000 INFO @ Thu, 16 Apr 2020 00:55:55: 28000000 INFO @ Thu, 16 Apr 2020 00:55:57: 33000000 INFO @ Thu, 16 Apr 2020 00:55:59: 24000000 INFO @ Thu, 16 Apr 2020 00:56:01: 29000000 INFO @ Thu, 16 Apr 2020 00:56:04: 34000000 INFO @ Thu, 16 Apr 2020 00:56:06: 25000000 INFO @ Thu, 16 Apr 2020 00:56:08: 30000000 INFO @ Thu, 16 Apr 2020 00:56:11: 35000000 INFO @ Thu, 16 Apr 2020 00:56:12: 26000000 INFO @ Thu, 16 Apr 2020 00:56:15: 31000000 INFO @ Thu, 16 Apr 2020 00:56:15: #1 tag size is determined as 76 bps INFO @ Thu, 16 Apr 2020 00:56:15: #1 tag size = 76 INFO @ Thu, 16 Apr 2020 00:56:15: #1 total tags in treatment: 35628057 INFO @ Thu, 16 Apr 2020 00:56:15: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:56:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:56:16: #1 tags after filtering in treatment: 35628057 INFO @ Thu, 16 Apr 2020 00:56:16: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 16 Apr 2020 00:56:16: #1 finished! INFO @ Thu, 16 Apr 2020 00:56:16: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:56:16: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:56:18: #2 number of paired peaks: 0 WARNING @ Thu, 16 Apr 2020 00:56:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 16 Apr 2020 00:56:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7971810/SRX7971810.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971810/SRX7971810.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971810/SRX7971810.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971810/SRX7971810.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:56:19: 27000000 INFO @ Thu, 16 Apr 2020 00:56:21: 32000000 INFO @ Thu, 16 Apr 2020 00:56:25: 28000000 INFO @ Thu, 16 Apr 2020 00:56:28: 33000000 INFO @ Thu, 16 Apr 2020 00:56:32: 29000000 INFO @ Thu, 16 Apr 2020 00:56:34: 34000000 INFO @ Thu, 16 Apr 2020 00:56:38: 30000000 INFO @ Thu, 16 Apr 2020 00:56:41: 35000000 INFO @ Thu, 16 Apr 2020 00:56:45: 31000000 INFO @ Thu, 16 Apr 2020 00:56:45: #1 tag size is determined as 76 bps INFO @ Thu, 16 Apr 2020 00:56:45: #1 tag size = 76 INFO @ Thu, 16 Apr 2020 00:56:45: #1 total tags in treatment: 35628057 INFO @ Thu, 16 Apr 2020 00:56:45: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:56:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:56:46: #1 tags after filtering in treatment: 35628057 INFO @ Thu, 16 Apr 2020 00:56:46: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 16 Apr 2020 00:56:46: #1 finished! INFO @ Thu, 16 Apr 2020 00:56:46: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:56:46: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:56:48: #2 number of paired peaks: 0 WARNING @ Thu, 16 Apr 2020 00:56:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 16 Apr 2020 00:56:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7971810/SRX7971810.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971810/SRX7971810.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971810/SRX7971810.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971810/SRX7971810.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:56:52: 32000000 INFO @ Thu, 16 Apr 2020 00:56:58: 33000000 INFO @ Thu, 16 Apr 2020 00:57:04: 34000000 INFO @ Thu, 16 Apr 2020 00:57:10: 35000000 INFO @ Thu, 16 Apr 2020 00:57:15: #1 tag size is determined as 76 bps INFO @ Thu, 16 Apr 2020 00:57:15: #1 tag size = 76 INFO @ Thu, 16 Apr 2020 00:57:15: #1 total tags in treatment: 35628057 INFO @ Thu, 16 Apr 2020 00:57:15: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:57:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:57:15: #1 tags after filtering in treatment: 35628057 INFO @ Thu, 16 Apr 2020 00:57:15: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 16 Apr 2020 00:57:15: #1 finished! INFO @ Thu, 16 Apr 2020 00:57:15: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:57:15: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:57:17: #2 number of paired peaks: 0 WARNING @ Thu, 16 Apr 2020 00:57:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 16 Apr 2020 00:57:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7971810/SRX7971810.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 6 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971810/SRX7971810.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971810/SRX7971810.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971810/SRX7971810.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。