Job ID = 5720349 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 27,314,673 reads read : 27,314,673 reads written : 27,314,673 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:17 27314673 reads; of these: 27314673 (100.00%) were unpaired; of these: 643402 (2.36%) aligned 0 times 24040139 (88.01%) aligned exactly 1 time 2631132 (9.63%) aligned >1 times 97.64% overall alignment rate Time searching: 00:08:17 Overall time: 00:08:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5709767 / 26671271 = 0.2141 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:21:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7971809/SRX7971809.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7971809/SRX7971809.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7971809/SRX7971809.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7971809/SRX7971809.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:21:28: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:21:28: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:21:34: 1000000 INFO @ Thu, 16 Apr 2020 00:21:40: 2000000 INFO @ Thu, 16 Apr 2020 00:21:45: 3000000 INFO @ Thu, 16 Apr 2020 00:21:51: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:21:57: 5000000 INFO @ Thu, 16 Apr 2020 00:21:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7971809/SRX7971809.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7971809/SRX7971809.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7971809/SRX7971809.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7971809/SRX7971809.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:21:58: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:21:58: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:22:03: 6000000 INFO @ Thu, 16 Apr 2020 00:22:04: 1000000 INFO @ Thu, 16 Apr 2020 00:22:09: 7000000 INFO @ Thu, 16 Apr 2020 00:22:10: 2000000 INFO @ Thu, 16 Apr 2020 00:22:15: 8000000 INFO @ Thu, 16 Apr 2020 00:22:16: 3000000 INFO @ Thu, 16 Apr 2020 00:22:21: 9000000 INFO @ Thu, 16 Apr 2020 00:22:22: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:22:26: 10000000 INFO @ Thu, 16 Apr 2020 00:22:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7971809/SRX7971809.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7971809/SRX7971809.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7971809/SRX7971809.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7971809/SRX7971809.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:22:28: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:22:28: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:22:28: 5000000 INFO @ Thu, 16 Apr 2020 00:22:32: 11000000 INFO @ Thu, 16 Apr 2020 00:22:34: 1000000 INFO @ Thu, 16 Apr 2020 00:22:34: 6000000 INFO @ Thu, 16 Apr 2020 00:22:38: 12000000 INFO @ Thu, 16 Apr 2020 00:22:40: 7000000 INFO @ Thu, 16 Apr 2020 00:22:41: 2000000 INFO @ Thu, 16 Apr 2020 00:22:44: 13000000 INFO @ Thu, 16 Apr 2020 00:22:46: 8000000 INFO @ Thu, 16 Apr 2020 00:22:47: 3000000 INFO @ Thu, 16 Apr 2020 00:22:50: 14000000 INFO @ Thu, 16 Apr 2020 00:22:52: 9000000 INFO @ Thu, 16 Apr 2020 00:22:53: 4000000 INFO @ Thu, 16 Apr 2020 00:22:55: 15000000 INFO @ Thu, 16 Apr 2020 00:22:58: 10000000 INFO @ Thu, 16 Apr 2020 00:22:59: 5000000 INFO @ Thu, 16 Apr 2020 00:23:01: 16000000 INFO @ Thu, 16 Apr 2020 00:23:04: 11000000 INFO @ Thu, 16 Apr 2020 00:23:05: 6000000 INFO @ Thu, 16 Apr 2020 00:23:07: 17000000 INFO @ Thu, 16 Apr 2020 00:23:10: 12000000 INFO @ Thu, 16 Apr 2020 00:23:11: 7000000 INFO @ Thu, 16 Apr 2020 00:23:12: 18000000 INFO @ Thu, 16 Apr 2020 00:23:16: 13000000 INFO @ Thu, 16 Apr 2020 00:23:17: 8000000 INFO @ Thu, 16 Apr 2020 00:23:18: 19000000 INFO @ Thu, 16 Apr 2020 00:23:21: 14000000 INFO @ Thu, 16 Apr 2020 00:23:23: 9000000 INFO @ Thu, 16 Apr 2020 00:23:24: 20000000 INFO @ Thu, 16 Apr 2020 00:23:27: 15000000 INFO @ Thu, 16 Apr 2020 00:23:29: 10000000 INFO @ Thu, 16 Apr 2020 00:23:29: #1 tag size is determined as 76 bps INFO @ Thu, 16 Apr 2020 00:23:29: #1 tag size = 76 INFO @ Thu, 16 Apr 2020 00:23:29: #1 total tags in treatment: 20961504 INFO @ Thu, 16 Apr 2020 00:23:29: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:23:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:23:30: #1 tags after filtering in treatment: 20961504 INFO @ Thu, 16 Apr 2020 00:23:30: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 16 Apr 2020 00:23:30: #1 finished! INFO @ Thu, 16 Apr 2020 00:23:30: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:23:30: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:23:31: #2 number of paired peaks: 55 WARNING @ Thu, 16 Apr 2020 00:23:31: Too few paired peaks (55) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 16 Apr 2020 00:23:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7971809/SRX7971809.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971809/SRX7971809.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971809/SRX7971809.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971809/SRX7971809.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:23:33: 16000000 INFO @ Thu, 16 Apr 2020 00:23:35: 11000000 INFO @ Thu, 16 Apr 2020 00:23:38: 17000000 INFO @ Thu, 16 Apr 2020 00:23:41: 12000000 INFO @ Thu, 16 Apr 2020 00:23:44: 18000000 INFO @ Thu, 16 Apr 2020 00:23:47: 13000000 INFO @ Thu, 16 Apr 2020 00:23:50: 19000000 INFO @ Thu, 16 Apr 2020 00:23:53: 14000000 INFO @ Thu, 16 Apr 2020 00:23:55: 20000000 INFO @ Thu, 16 Apr 2020 00:23:58: 15000000 INFO @ Thu, 16 Apr 2020 00:24:01: #1 tag size is determined as 76 bps INFO @ Thu, 16 Apr 2020 00:24:01: #1 tag size = 76 INFO @ Thu, 16 Apr 2020 00:24:01: #1 total tags in treatment: 20961504 INFO @ Thu, 16 Apr 2020 00:24:01: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:24:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:24:01: #1 tags after filtering in treatment: 20961504 INFO @ Thu, 16 Apr 2020 00:24:01: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 16 Apr 2020 00:24:01: #1 finished! INFO @ Thu, 16 Apr 2020 00:24:01: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:24:01: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:24:03: #2 number of paired peaks: 55 WARNING @ Thu, 16 Apr 2020 00:24:03: Too few paired peaks (55) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 16 Apr 2020 00:24:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7971809/SRX7971809.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971809/SRX7971809.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971809/SRX7971809.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971809/SRX7971809.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:24:04: 16000000 INFO @ Thu, 16 Apr 2020 00:24:10: 17000000 INFO @ Thu, 16 Apr 2020 00:24:15: 18000000 INFO @ Thu, 16 Apr 2020 00:24:21: 19000000 INFO @ Thu, 16 Apr 2020 00:24:27: 20000000 INFO @ Thu, 16 Apr 2020 00:24:32: #1 tag size is determined as 76 bps INFO @ Thu, 16 Apr 2020 00:24:32: #1 tag size = 76 INFO @ Thu, 16 Apr 2020 00:24:32: #1 total tags in treatment: 20961504 INFO @ Thu, 16 Apr 2020 00:24:32: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:24:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:24:32: #1 tags after filtering in treatment: 20961504 INFO @ Thu, 16 Apr 2020 00:24:32: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 16 Apr 2020 00:24:32: #1 finished! INFO @ Thu, 16 Apr 2020 00:24:32: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:24:32: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:24:34: #2 number of paired peaks: 55 WARNING @ Thu, 16 Apr 2020 00:24:34: Too few paired peaks (55) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 16 Apr 2020 00:24:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7971809/SRX7971809.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971809/SRX7971809.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971809/SRX7971809.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971809/SRX7971809.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。