Job ID = 14157849 SRX = SRX7879257 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 44815642 spots for SRR11272872/SRR11272872.sra Written 44815642 spots for SRR11272872/SRR11272872.sra fastq に変換しました。 bowtie でマッピング中... Your job 14157993 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:42 44815642 reads; of these: 44815642 (100.00%) were unpaired; of these: 454065 (1.01%) aligned 0 times 37670422 (84.06%) aligned exactly 1 time 6691155 (14.93%) aligned >1 times 98.99% overall alignment rate Time searching: 00:09:42 Overall time: 00:09:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8399309 / 44361577 = 0.1893 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 11:45:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 11:45:39: #1 read tag files... INFO @ Wed, 08 Dec 2021 11:45:39: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 11:45:44: 1000000 INFO @ Wed, 08 Dec 2021 11:45:49: 2000000 INFO @ Wed, 08 Dec 2021 11:45:54: 3000000 INFO @ Wed, 08 Dec 2021 11:45:59: 4000000 INFO @ Wed, 08 Dec 2021 11:46:04: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 11:46:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 11:46:09: #1 read tag files... INFO @ Wed, 08 Dec 2021 11:46:09: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 11:46:10: 6000000 INFO @ Wed, 08 Dec 2021 11:46:15: 7000000 INFO @ Wed, 08 Dec 2021 11:46:16: 1000000 INFO @ Wed, 08 Dec 2021 11:46:21: 8000000 INFO @ Wed, 08 Dec 2021 11:46:22: 2000000 INFO @ Wed, 08 Dec 2021 11:46:27: 9000000 INFO @ Wed, 08 Dec 2021 11:46:29: 3000000 INFO @ Wed, 08 Dec 2021 11:46:32: 10000000 INFO @ Wed, 08 Dec 2021 11:46:35: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 11:46:38: 11000000 INFO @ Wed, 08 Dec 2021 11:46:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 11:46:39: #1 read tag files... INFO @ Wed, 08 Dec 2021 11:46:39: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 11:46:42: 5000000 INFO @ Wed, 08 Dec 2021 11:46:44: 12000000 INFO @ Wed, 08 Dec 2021 11:46:45: 1000000 INFO @ Wed, 08 Dec 2021 11:46:48: 6000000 INFO @ Wed, 08 Dec 2021 11:46:50: 13000000 INFO @ Wed, 08 Dec 2021 11:46:51: 2000000 INFO @ Wed, 08 Dec 2021 11:46:55: 7000000 INFO @ Wed, 08 Dec 2021 11:46:56: 14000000 INFO @ Wed, 08 Dec 2021 11:46:57: 3000000 INFO @ Wed, 08 Dec 2021 11:47:01: 8000000 INFO @ Wed, 08 Dec 2021 11:47:01: 15000000 INFO @ Wed, 08 Dec 2021 11:47:03: 4000000 INFO @ Wed, 08 Dec 2021 11:47:07: 16000000 INFO @ Wed, 08 Dec 2021 11:47:07: 9000000 INFO @ Wed, 08 Dec 2021 11:47:09: 5000000 INFO @ Wed, 08 Dec 2021 11:47:13: 17000000 INFO @ Wed, 08 Dec 2021 11:47:14: 10000000 INFO @ Wed, 08 Dec 2021 11:47:15: 6000000 INFO @ Wed, 08 Dec 2021 11:47:19: 18000000 INFO @ Wed, 08 Dec 2021 11:47:20: 11000000 INFO @ Wed, 08 Dec 2021 11:47:21: 7000000 INFO @ Wed, 08 Dec 2021 11:47:25: 19000000 INFO @ Wed, 08 Dec 2021 11:47:26: 12000000 INFO @ Wed, 08 Dec 2021 11:47:27: 8000000 INFO @ Wed, 08 Dec 2021 11:47:31: 20000000 INFO @ Wed, 08 Dec 2021 11:47:33: 13000000 INFO @ Wed, 08 Dec 2021 11:47:33: 9000000 INFO @ Wed, 08 Dec 2021 11:47:37: 21000000 INFO @ Wed, 08 Dec 2021 11:47:39: 10000000 INFO @ Wed, 08 Dec 2021 11:47:39: 14000000 INFO @ Wed, 08 Dec 2021 11:47:42: 22000000 INFO @ Wed, 08 Dec 2021 11:47:45: 11000000 INFO @ Wed, 08 Dec 2021 11:47:45: 15000000 INFO @ Wed, 08 Dec 2021 11:47:48: 23000000 INFO @ Wed, 08 Dec 2021 11:47:50: 12000000 INFO @ Wed, 08 Dec 2021 11:47:52: 16000000 INFO @ Wed, 08 Dec 2021 11:47:54: 24000000 INFO @ Wed, 08 Dec 2021 11:47:56: 13000000 INFO @ Wed, 08 Dec 2021 11:47:58: 17000000 INFO @ Wed, 08 Dec 2021 11:48:00: 25000000 INFO @ Wed, 08 Dec 2021 11:48:02: 14000000 INFO @ Wed, 08 Dec 2021 11:48:05: 18000000 INFO @ Wed, 08 Dec 2021 11:48:05: 26000000 INFO @ Wed, 08 Dec 2021 11:48:08: 15000000 INFO @ Wed, 08 Dec 2021 11:48:11: 19000000 INFO @ Wed, 08 Dec 2021 11:48:11: 27000000 INFO @ Wed, 08 Dec 2021 11:48:14: 16000000 INFO @ Wed, 08 Dec 2021 11:48:17: 28000000 INFO @ Wed, 08 Dec 2021 11:48:18: 20000000 INFO @ Wed, 08 Dec 2021 11:48:20: 17000000 INFO @ Wed, 08 Dec 2021 11:48:23: 29000000 INFO @ Wed, 08 Dec 2021 11:48:24: 21000000 INFO @ Wed, 08 Dec 2021 11:48:26: 18000000 INFO @ Wed, 08 Dec 2021 11:48:28: 30000000 INFO @ Wed, 08 Dec 2021 11:48:30: 22000000 INFO @ Wed, 08 Dec 2021 11:48:32: 19000000 INFO @ Wed, 08 Dec 2021 11:48:34: 31000000 INFO @ Wed, 08 Dec 2021 11:48:37: 23000000 INFO @ Wed, 08 Dec 2021 11:48:38: 20000000 INFO @ Wed, 08 Dec 2021 11:48:40: 32000000 INFO @ Wed, 08 Dec 2021 11:48:43: 24000000 INFO @ Wed, 08 Dec 2021 11:48:43: 21000000 INFO @ Wed, 08 Dec 2021 11:48:45: 33000000 INFO @ Wed, 08 Dec 2021 11:48:49: 25000000 INFO @ Wed, 08 Dec 2021 11:48:49: 22000000 INFO @ Wed, 08 Dec 2021 11:48:51: 34000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 11:48:55: 23000000 INFO @ Wed, 08 Dec 2021 11:48:55: 26000000 INFO @ Wed, 08 Dec 2021 11:48:57: 35000000 INFO @ Wed, 08 Dec 2021 11:49:01: 24000000 INFO @ Wed, 08 Dec 2021 11:49:02: 27000000 INFO @ Wed, 08 Dec 2021 11:49:03: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 11:49:03: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 11:49:03: #1 total tags in treatment: 35962268 INFO @ Wed, 08 Dec 2021 11:49:03: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 11:49:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 11:49:04: #1 tags after filtering in treatment: 35962268 INFO @ Wed, 08 Dec 2021 11:49:04: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 11:49:04: #1 finished! INFO @ Wed, 08 Dec 2021 11:49:04: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 11:49:04: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 11:49:06: #2 number of paired peaks: 0 WARNING @ Wed, 08 Dec 2021 11:49:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 11:49:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 11:49:07: 25000000 INFO @ Wed, 08 Dec 2021 11:49:08: 28000000 INFO @ Wed, 08 Dec 2021 11:49:13: 26000000 INFO @ Wed, 08 Dec 2021 11:49:14: 29000000 INFO @ Wed, 08 Dec 2021 11:49:19: 27000000 INFO @ Wed, 08 Dec 2021 11:49:20: 30000000 INFO @ Wed, 08 Dec 2021 11:49:24: 28000000 INFO @ Wed, 08 Dec 2021 11:49:26: 31000000 INFO @ Wed, 08 Dec 2021 11:49:30: 29000000 INFO @ Wed, 08 Dec 2021 11:49:33: 32000000 INFO @ Wed, 08 Dec 2021 11:49:36: 30000000 INFO @ Wed, 08 Dec 2021 11:49:39: 33000000 INFO @ Wed, 08 Dec 2021 11:49:41: 31000000 INFO @ Wed, 08 Dec 2021 11:49:46: 34000000 INFO @ Wed, 08 Dec 2021 11:49:47: 32000000 INFO @ Wed, 08 Dec 2021 11:49:52: 35000000 INFO @ Wed, 08 Dec 2021 11:49:53: 33000000 BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 11:49:58: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 11:49:58: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 11:49:58: #1 total tags in treatment: 35962268 INFO @ Wed, 08 Dec 2021 11:49:58: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 11:49:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 11:49:59: #1 tags after filtering in treatment: 35962268 INFO @ Wed, 08 Dec 2021 11:49:59: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 11:49:59: #1 finished! INFO @ Wed, 08 Dec 2021 11:49:59: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 11:49:59: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 11:49:59: 34000000 INFO @ Wed, 08 Dec 2021 11:50:01: #2 number of paired peaks: 0 WARNING @ Wed, 08 Dec 2021 11:50:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 11:50:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 11:50:04: 35000000 INFO @ Wed, 08 Dec 2021 11:50:10: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 11:50:10: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 11:50:10: #1 total tags in treatment: 35962268 INFO @ Wed, 08 Dec 2021 11:50:10: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 11:50:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 11:50:10: #1 tags after filtering in treatment: 35962268 INFO @ Wed, 08 Dec 2021 11:50:10: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 11:50:10: #1 finished! INFO @ Wed, 08 Dec 2021 11:50:10: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 11:50:10: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 11:50:12: #2 number of paired peaks: 0 WARNING @ Wed, 08 Dec 2021 11:50:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 11:50:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling