Job ID = 10165650 SRX = SRX7879257 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 44815642 spots for SRR11272872/SRR11272872.sra Written 44815642 spots for SRR11272872/SRR11272872.sra fastq に変換しました。 bowtie でマッピング中... Your job 10165951 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:07 44815642 reads; of these: 44815642 (100.00%) were unpaired; of these: 454065 (1.01%) aligned 0 times 37670422 (84.06%) aligned exactly 1 time 6691155 (14.93%) aligned >1 times 98.99% overall alignment rate Time searching: 00:10:07 Overall time: 00:10:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8399309 / 44361577 = 0.1893 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:53:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:53:38: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:53:38: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:53:44: 1000000 INFO @ Thu, 08 Oct 2020 19:53:51: 2000000 INFO @ Thu, 08 Oct 2020 19:53:57: 3000000 INFO @ Thu, 08 Oct 2020 19:54:03: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:54:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:54:08: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:54:08: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:54:09: 5000000 INFO @ Thu, 08 Oct 2020 19:54:15: 1000000 INFO @ Thu, 08 Oct 2020 19:54:15: 6000000 INFO @ Thu, 08 Oct 2020 19:54:21: 2000000 INFO @ Thu, 08 Oct 2020 19:54:22: 7000000 INFO @ Thu, 08 Oct 2020 19:54:27: 3000000 INFO @ Thu, 08 Oct 2020 19:54:28: 8000000 INFO @ Thu, 08 Oct 2020 19:54:33: 4000000 INFO @ Thu, 08 Oct 2020 19:54:34: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:54:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:54:38: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:54:38: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:54:40: 5000000 INFO @ Thu, 08 Oct 2020 19:54:41: 10000000 INFO @ Thu, 08 Oct 2020 19:54:44: 1000000 INFO @ Thu, 08 Oct 2020 19:54:46: 6000000 INFO @ Thu, 08 Oct 2020 19:54:47: 11000000 INFO @ Thu, 08 Oct 2020 19:54:50: 2000000 INFO @ Thu, 08 Oct 2020 19:54:52: 7000000 INFO @ Thu, 08 Oct 2020 19:54:54: 12000000 INFO @ Thu, 08 Oct 2020 19:54:56: 3000000 INFO @ Thu, 08 Oct 2020 19:54:59: 8000000 INFO @ Thu, 08 Oct 2020 19:55:00: 13000000 INFO @ Thu, 08 Oct 2020 19:55:02: 4000000 INFO @ Thu, 08 Oct 2020 19:55:05: 9000000 INFO @ Thu, 08 Oct 2020 19:55:07: 14000000 INFO @ Thu, 08 Oct 2020 19:55:08: 5000000 INFO @ Thu, 08 Oct 2020 19:55:12: 10000000 INFO @ Thu, 08 Oct 2020 19:55:13: 15000000 INFO @ Thu, 08 Oct 2020 19:55:13: 6000000 INFO @ Thu, 08 Oct 2020 19:55:18: 11000000 INFO @ Thu, 08 Oct 2020 19:55:19: 7000000 INFO @ Thu, 08 Oct 2020 19:55:19: 16000000 INFO @ Thu, 08 Oct 2020 19:55:25: 12000000 INFO @ Thu, 08 Oct 2020 19:55:25: 8000000 INFO @ Thu, 08 Oct 2020 19:55:26: 17000000 INFO @ Thu, 08 Oct 2020 19:55:31: 13000000 INFO @ Thu, 08 Oct 2020 19:55:31: 9000000 INFO @ Thu, 08 Oct 2020 19:55:32: 18000000 INFO @ Thu, 08 Oct 2020 19:55:37: 10000000 INFO @ Thu, 08 Oct 2020 19:55:37: 14000000 INFO @ Thu, 08 Oct 2020 19:55:38: 19000000 INFO @ Thu, 08 Oct 2020 19:55:43: 11000000 INFO @ Thu, 08 Oct 2020 19:55:44: 15000000 INFO @ Thu, 08 Oct 2020 19:55:45: 20000000 INFO @ Thu, 08 Oct 2020 19:55:49: 12000000 INFO @ Thu, 08 Oct 2020 19:55:50: 16000000 INFO @ Thu, 08 Oct 2020 19:55:51: 21000000 INFO @ Thu, 08 Oct 2020 19:55:55: 13000000 INFO @ Thu, 08 Oct 2020 19:55:56: 17000000 INFO @ Thu, 08 Oct 2020 19:55:57: 22000000 INFO @ Thu, 08 Oct 2020 19:56:01: 14000000 INFO @ Thu, 08 Oct 2020 19:56:03: 18000000 INFO @ Thu, 08 Oct 2020 19:56:04: 23000000 INFO @ Thu, 08 Oct 2020 19:56:07: 15000000 INFO @ Thu, 08 Oct 2020 19:56:09: 19000000 INFO @ Thu, 08 Oct 2020 19:56:10: 24000000 INFO @ Thu, 08 Oct 2020 19:56:12: 16000000 INFO @ Thu, 08 Oct 2020 19:56:15: 20000000 INFO @ Thu, 08 Oct 2020 19:56:16: 25000000 INFO @ Thu, 08 Oct 2020 19:56:18: 17000000 INFO @ Thu, 08 Oct 2020 19:56:21: 21000000 INFO @ Thu, 08 Oct 2020 19:56:23: 26000000 INFO @ Thu, 08 Oct 2020 19:56:24: 18000000 INFO @ Thu, 08 Oct 2020 19:56:27: 22000000 INFO @ Thu, 08 Oct 2020 19:56:29: 27000000 INFO @ Thu, 08 Oct 2020 19:56:29: 19000000 INFO @ Thu, 08 Oct 2020 19:56:33: 23000000 INFO @ Thu, 08 Oct 2020 19:56:35: 20000000 INFO @ Thu, 08 Oct 2020 19:56:35: 28000000 INFO @ Thu, 08 Oct 2020 19:56:39: 24000000 INFO @ Thu, 08 Oct 2020 19:56:40: 21000000 INFO @ Thu, 08 Oct 2020 19:56:41: 29000000 INFO @ Thu, 08 Oct 2020 19:56:45: 25000000 INFO @ Thu, 08 Oct 2020 19:56:46: 22000000 INFO @ Thu, 08 Oct 2020 19:56:48: 30000000 INFO @ Thu, 08 Oct 2020 19:56:52: 26000000 INFO @ Thu, 08 Oct 2020 19:56:52: 23000000 INFO @ Thu, 08 Oct 2020 19:56:54: 31000000 INFO @ Thu, 08 Oct 2020 19:56:57: 24000000 INFO @ Thu, 08 Oct 2020 19:56:58: 27000000 INFO @ Thu, 08 Oct 2020 19:57:00: 32000000 INFO @ Thu, 08 Oct 2020 19:57:03: 25000000 INFO @ Thu, 08 Oct 2020 19:57:04: 28000000 INFO @ Thu, 08 Oct 2020 19:57:07: 33000000 INFO @ Thu, 08 Oct 2020 19:57:09: 26000000 INFO @ Thu, 08 Oct 2020 19:57:10: 29000000 INFO @ Thu, 08 Oct 2020 19:57:13: 34000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 19:57:14: 27000000 INFO @ Thu, 08 Oct 2020 19:57:16: 30000000 INFO @ Thu, 08 Oct 2020 19:57:19: 35000000 INFO @ Thu, 08 Oct 2020 19:57:20: 28000000 INFO @ Thu, 08 Oct 2020 19:57:23: 31000000 INFO @ Thu, 08 Oct 2020 19:57:25: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:57:25: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:57:25: #1 total tags in treatment: 35962268 INFO @ Thu, 08 Oct 2020 19:57:25: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:57:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:57:25: 29000000 INFO @ Thu, 08 Oct 2020 19:57:26: #1 tags after filtering in treatment: 35962268 INFO @ Thu, 08 Oct 2020 19:57:26: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:57:26: #1 finished! INFO @ Thu, 08 Oct 2020 19:57:26: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:57:26: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:57:28: #2 number of paired peaks: 0 WARNING @ Thu, 08 Oct 2020 19:57:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:57:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 19:57:29: 32000000 INFO @ Thu, 08 Oct 2020 19:57:31: 30000000 INFO @ Thu, 08 Oct 2020 19:57:35: 33000000 INFO @ Thu, 08 Oct 2020 19:57:37: 31000000 INFO @ Thu, 08 Oct 2020 19:57:41: 34000000 INFO @ Thu, 08 Oct 2020 19:57:43: 32000000 INFO @ Thu, 08 Oct 2020 19:57:47: 35000000 INFO @ Thu, 08 Oct 2020 19:57:48: 33000000 INFO @ Thu, 08 Oct 2020 19:57:53: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:57:53: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:57:53: #1 total tags in treatment: 35962268 INFO @ Thu, 08 Oct 2020 19:57:53: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:57:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:57:54: #1 tags after filtering in treatment: 35962268 INFO @ Thu, 08 Oct 2020 19:57:54: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:57:54: #1 finished! INFO @ Thu, 08 Oct 2020 19:57:54: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:57:54: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:57:54: 34000000 INFO @ Thu, 08 Oct 2020 19:57:56: #2 number of paired peaks: 0 WARNING @ Thu, 08 Oct 2020 19:57:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:57:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 19:58:00: 35000000 INFO @ Thu, 08 Oct 2020 19:58:05: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:58:05: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:58:05: #1 total tags in treatment: 35962268 INFO @ Thu, 08 Oct 2020 19:58:05: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:58:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:58:06: #1 tags after filtering in treatment: 35962268 INFO @ Thu, 08 Oct 2020 19:58:06: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:58:06: #1 finished! INFO @ Thu, 08 Oct 2020 19:58:06: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:58:06: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:58:08: #2 number of paired peaks: 0 WARNING @ Thu, 08 Oct 2020 19:58:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:58:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879257/SRX7879257.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。