Job ID = 10165649 SRX = SRX7879256 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 40235808 spots for SRR11272871/SRR11272871.sra Written 40235808 spots for SRR11272871/SRR11272871.sra fastq に変換しました。 bowtie でマッピング中... Your job 10165931 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:51 40235808 reads; of these: 40235808 (100.00%) were unpaired; of these: 402048 (1.00%) aligned 0 times 33825242 (84.07%) aligned exactly 1 time 6008518 (14.93%) aligned >1 times 99.00% overall alignment rate Time searching: 00:08:51 Overall time: 00:08:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 6784034 / 39833760 = 0.1703 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:51:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:51:13: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:51:13: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:51:19: 1000000 INFO @ Thu, 08 Oct 2020 19:51:26: 2000000 INFO @ Thu, 08 Oct 2020 19:51:32: 3000000 INFO @ Thu, 08 Oct 2020 19:51:39: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:51:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:51:42: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:51:42: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:51:46: 5000000 INFO @ Thu, 08 Oct 2020 19:51:49: 1000000 INFO @ Thu, 08 Oct 2020 19:51:53: 6000000 INFO @ Thu, 08 Oct 2020 19:51:55: 2000000 INFO @ Thu, 08 Oct 2020 19:52:00: 7000000 INFO @ Thu, 08 Oct 2020 19:52:02: 3000000 INFO @ Thu, 08 Oct 2020 19:52:07: 8000000 INFO @ Thu, 08 Oct 2020 19:52:08: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:52:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:52:12: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:52:12: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:52:14: 9000000 INFO @ Thu, 08 Oct 2020 19:52:15: 5000000 INFO @ Thu, 08 Oct 2020 19:52:19: 1000000 INFO @ Thu, 08 Oct 2020 19:52:21: 10000000 INFO @ Thu, 08 Oct 2020 19:52:21: 6000000 INFO @ Thu, 08 Oct 2020 19:52:26: 2000000 INFO @ Thu, 08 Oct 2020 19:52:28: 7000000 INFO @ Thu, 08 Oct 2020 19:52:28: 11000000 INFO @ Thu, 08 Oct 2020 19:52:32: 3000000 INFO @ Thu, 08 Oct 2020 19:52:35: 8000000 INFO @ Thu, 08 Oct 2020 19:52:36: 12000000 INFO @ Thu, 08 Oct 2020 19:52:39: 4000000 INFO @ Thu, 08 Oct 2020 19:52:41: 9000000 INFO @ Thu, 08 Oct 2020 19:52:43: 13000000 INFO @ Thu, 08 Oct 2020 19:52:46: 5000000 INFO @ Thu, 08 Oct 2020 19:52:48: 10000000 INFO @ Thu, 08 Oct 2020 19:52:50: 14000000 INFO @ Thu, 08 Oct 2020 19:52:53: 6000000 INFO @ Thu, 08 Oct 2020 19:52:55: 11000000 INFO @ Thu, 08 Oct 2020 19:52:57: 15000000 INFO @ Thu, 08 Oct 2020 19:53:00: 7000000 INFO @ Thu, 08 Oct 2020 19:53:02: 12000000 INFO @ Thu, 08 Oct 2020 19:53:04: 16000000 INFO @ Thu, 08 Oct 2020 19:53:06: 8000000 INFO @ Thu, 08 Oct 2020 19:53:09: 13000000 INFO @ Thu, 08 Oct 2020 19:53:11: 17000000 INFO @ Thu, 08 Oct 2020 19:53:13: 9000000 INFO @ Thu, 08 Oct 2020 19:53:15: 14000000 INFO @ Thu, 08 Oct 2020 19:53:18: 18000000 INFO @ Thu, 08 Oct 2020 19:53:20: 10000000 INFO @ Thu, 08 Oct 2020 19:53:22: 15000000 INFO @ Thu, 08 Oct 2020 19:53:25: 19000000 INFO @ Thu, 08 Oct 2020 19:53:27: 11000000 INFO @ Thu, 08 Oct 2020 19:53:29: 16000000 INFO @ Thu, 08 Oct 2020 19:53:32: 20000000 INFO @ Thu, 08 Oct 2020 19:53:34: 12000000 INFO @ Thu, 08 Oct 2020 19:53:36: 17000000 INFO @ Thu, 08 Oct 2020 19:53:39: 21000000 INFO @ Thu, 08 Oct 2020 19:53:40: 13000000 INFO @ Thu, 08 Oct 2020 19:53:42: 18000000 INFO @ Thu, 08 Oct 2020 19:53:47: 22000000 INFO @ Thu, 08 Oct 2020 19:53:47: 14000000 INFO @ Thu, 08 Oct 2020 19:53:49: 19000000 INFO @ Thu, 08 Oct 2020 19:53:54: 15000000 INFO @ Thu, 08 Oct 2020 19:53:54: 23000000 INFO @ Thu, 08 Oct 2020 19:53:56: 20000000 INFO @ Thu, 08 Oct 2020 19:54:00: 16000000 INFO @ Thu, 08 Oct 2020 19:54:01: 24000000 INFO @ Thu, 08 Oct 2020 19:54:03: 21000000 INFO @ Thu, 08 Oct 2020 19:54:07: 17000000 INFO @ Thu, 08 Oct 2020 19:54:08: 25000000 INFO @ Thu, 08 Oct 2020 19:54:10: 22000000 INFO @ Thu, 08 Oct 2020 19:54:14: 18000000 INFO @ Thu, 08 Oct 2020 19:54:15: 26000000 INFO @ Thu, 08 Oct 2020 19:54:16: 23000000 INFO @ Thu, 08 Oct 2020 19:54:20: 19000000 INFO @ Thu, 08 Oct 2020 19:54:22: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 19:54:23: 24000000 INFO @ Thu, 08 Oct 2020 19:54:27: 20000000 INFO @ Thu, 08 Oct 2020 19:54:29: 28000000 INFO @ Thu, 08 Oct 2020 19:54:30: 25000000 INFO @ Thu, 08 Oct 2020 19:54:34: 21000000 INFO @ Thu, 08 Oct 2020 19:54:36: 29000000 INFO @ Thu, 08 Oct 2020 19:54:36: 26000000 INFO @ Thu, 08 Oct 2020 19:54:41: 22000000 INFO @ Thu, 08 Oct 2020 19:54:43: 30000000 INFO @ Thu, 08 Oct 2020 19:54:43: 27000000 INFO @ Thu, 08 Oct 2020 19:54:47: 23000000 INFO @ Thu, 08 Oct 2020 19:54:49: 28000000 INFO @ Thu, 08 Oct 2020 19:54:50: 31000000 INFO @ Thu, 08 Oct 2020 19:54:54: 24000000 INFO @ Thu, 08 Oct 2020 19:54:56: 29000000 INFO @ Thu, 08 Oct 2020 19:54:57: 32000000 INFO @ Thu, 08 Oct 2020 19:55:01: 25000000 INFO @ Thu, 08 Oct 2020 19:55:03: 30000000 INFO @ Thu, 08 Oct 2020 19:55:03: 33000000 INFO @ Thu, 08 Oct 2020 19:55:04: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:55:04: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:55:04: #1 total tags in treatment: 33049726 INFO @ Thu, 08 Oct 2020 19:55:04: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:55:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:55:04: #1 tags after filtering in treatment: 33049726 INFO @ Thu, 08 Oct 2020 19:55:04: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:55:04: #1 finished! INFO @ Thu, 08 Oct 2020 19:55:04: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:55:04: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:55:06: #2 number of paired peaks: 19 WARNING @ Thu, 08 Oct 2020 19:55:06: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:55:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 19:55:07: 26000000 INFO @ Thu, 08 Oct 2020 19:55:09: 31000000 INFO @ Thu, 08 Oct 2020 19:55:13: 27000000 INFO @ Thu, 08 Oct 2020 19:55:15: 32000000 INFO @ Thu, 08 Oct 2020 19:55:19: 28000000 INFO @ Thu, 08 Oct 2020 19:55:21: 33000000 INFO @ Thu, 08 Oct 2020 19:55:22: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:55:22: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:55:22: #1 total tags in treatment: 33049726 INFO @ Thu, 08 Oct 2020 19:55:22: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:55:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) BigWig に変換しました。 INFO @ Thu, 08 Oct 2020 19:55:22: #1 tags after filtering in treatment: 33049726 INFO @ Thu, 08 Oct 2020 19:55:22: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:55:22: #1 finished! INFO @ Thu, 08 Oct 2020 19:55:22: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:55:22: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:55:24: #2 number of paired peaks: 19 WARNING @ Thu, 08 Oct 2020 19:55:24: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:55:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 19:55:25: 29000000 INFO @ Thu, 08 Oct 2020 19:55:31: 30000000 INFO @ Thu, 08 Oct 2020 19:55:36: 31000000 INFO @ Thu, 08 Oct 2020 19:55:42: 32000000 INFO @ Thu, 08 Oct 2020 19:55:47: 33000000 INFO @ Thu, 08 Oct 2020 19:55:48: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:55:48: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:55:48: #1 total tags in treatment: 33049726 INFO @ Thu, 08 Oct 2020 19:55:48: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:55:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:55:48: #1 tags after filtering in treatment: 33049726 INFO @ Thu, 08 Oct 2020 19:55:48: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:55:48: #1 finished! INFO @ Thu, 08 Oct 2020 19:55:48: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:55:48: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:55:50: #2 number of paired peaks: 19 WARNING @ Thu, 08 Oct 2020 19:55:50: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:55:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling