Job ID = 14157848 SRX = SRX7879256 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 40235808 spots for SRR11272871/SRR11272871.sra Written 40235808 spots for SRR11272871/SRR11272871.sra fastq に変換しました。 bowtie でマッピング中... Your job 14157987 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:09:08 40235808 reads; of these: 40235808 (100.00%) were unpaired; of these: 402048 (1.00%) aligned 0 times 33825242 (84.07%) aligned exactly 1 time 6008518 (14.93%) aligned >1 times 99.00% overall alignment rate Time searching: 00:09:09 Overall time: 00:09:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 6784034 / 39833760 = 0.1703 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 11:41:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 11:41:27: #1 read tag files... INFO @ Wed, 08 Dec 2021 11:41:27: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 11:41:34: 1000000 INFO @ Wed, 08 Dec 2021 11:41:40: 2000000 INFO @ Wed, 08 Dec 2021 11:41:47: 3000000 INFO @ Wed, 08 Dec 2021 11:41:54: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 11:41:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 11:41:56: #1 read tag files... INFO @ Wed, 08 Dec 2021 11:41:56: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 11:42:00: 5000000 INFO @ Wed, 08 Dec 2021 11:42:04: 1000000 INFO @ Wed, 08 Dec 2021 11:42:08: 6000000 INFO @ Wed, 08 Dec 2021 11:42:11: 2000000 INFO @ Wed, 08 Dec 2021 11:42:15: 7000000 INFO @ Wed, 08 Dec 2021 11:42:18: 3000000 INFO @ Wed, 08 Dec 2021 11:42:22: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 11:42:25: 4000000 INFO @ Wed, 08 Dec 2021 11:42:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 11:42:26: #1 read tag files... INFO @ Wed, 08 Dec 2021 11:42:26: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 11:42:29: 9000000 INFO @ Wed, 08 Dec 2021 11:42:32: 5000000 INFO @ Wed, 08 Dec 2021 11:42:33: 1000000 INFO @ Wed, 08 Dec 2021 11:42:36: 10000000 INFO @ Wed, 08 Dec 2021 11:42:39: 6000000 INFO @ Wed, 08 Dec 2021 11:42:40: 2000000 INFO @ Wed, 08 Dec 2021 11:42:43: 11000000 INFO @ Wed, 08 Dec 2021 11:42:46: 3000000 INFO @ Wed, 08 Dec 2021 11:42:46: 7000000 INFO @ Wed, 08 Dec 2021 11:42:50: 12000000 INFO @ Wed, 08 Dec 2021 11:42:53: 4000000 INFO @ Wed, 08 Dec 2021 11:42:54: 8000000 INFO @ Wed, 08 Dec 2021 11:42:57: 13000000 INFO @ Wed, 08 Dec 2021 11:42:59: 5000000 INFO @ Wed, 08 Dec 2021 11:43:01: 9000000 INFO @ Wed, 08 Dec 2021 11:43:04: 14000000 INFO @ Wed, 08 Dec 2021 11:43:06: 6000000 INFO @ Wed, 08 Dec 2021 11:43:08: 10000000 INFO @ Wed, 08 Dec 2021 11:43:11: 15000000 INFO @ Wed, 08 Dec 2021 11:43:12: 7000000 INFO @ Wed, 08 Dec 2021 11:43:15: 11000000 INFO @ Wed, 08 Dec 2021 11:43:18: 16000000 INFO @ Wed, 08 Dec 2021 11:43:19: 8000000 INFO @ Wed, 08 Dec 2021 11:43:22: 12000000 INFO @ Wed, 08 Dec 2021 11:43:25: 9000000 INFO @ Wed, 08 Dec 2021 11:43:25: 17000000 INFO @ Wed, 08 Dec 2021 11:43:29: 13000000 INFO @ Wed, 08 Dec 2021 11:43:31: 10000000 INFO @ Wed, 08 Dec 2021 11:43:33: 18000000 INFO @ Wed, 08 Dec 2021 11:43:36: 14000000 INFO @ Wed, 08 Dec 2021 11:43:38: 11000000 INFO @ Wed, 08 Dec 2021 11:43:40: 19000000 INFO @ Wed, 08 Dec 2021 11:43:43: 15000000 INFO @ Wed, 08 Dec 2021 11:43:44: 12000000 INFO @ Wed, 08 Dec 2021 11:43:47: 20000000 INFO @ Wed, 08 Dec 2021 11:43:50: 16000000 INFO @ Wed, 08 Dec 2021 11:43:51: 13000000 INFO @ Wed, 08 Dec 2021 11:43:54: 21000000 INFO @ Wed, 08 Dec 2021 11:43:57: 17000000 INFO @ Wed, 08 Dec 2021 11:43:57: 14000000 INFO @ Wed, 08 Dec 2021 11:44:01: 22000000 INFO @ Wed, 08 Dec 2021 11:44:04: 15000000 INFO @ Wed, 08 Dec 2021 11:44:04: 18000000 INFO @ Wed, 08 Dec 2021 11:44:08: 23000000 INFO @ Wed, 08 Dec 2021 11:44:10: 16000000 INFO @ Wed, 08 Dec 2021 11:44:11: 19000000 INFO @ Wed, 08 Dec 2021 11:44:15: 24000000 INFO @ Wed, 08 Dec 2021 11:44:17: 17000000 INFO @ Wed, 08 Dec 2021 11:44:18: 20000000 INFO @ Wed, 08 Dec 2021 11:44:22: 25000000 INFO @ Wed, 08 Dec 2021 11:44:23: 18000000 INFO @ Wed, 08 Dec 2021 11:44:25: 21000000 INFO @ Wed, 08 Dec 2021 11:44:29: 26000000 INFO @ Wed, 08 Dec 2021 11:44:30: 19000000 INFO @ Wed, 08 Dec 2021 11:44:32: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 11:44:36: 20000000 INFO @ Wed, 08 Dec 2021 11:44:36: 27000000 INFO @ Wed, 08 Dec 2021 11:44:40: 23000000 INFO @ Wed, 08 Dec 2021 11:44:43: 21000000 INFO @ Wed, 08 Dec 2021 11:44:43: 28000000 INFO @ Wed, 08 Dec 2021 11:44:47: 24000000 INFO @ Wed, 08 Dec 2021 11:44:49: 22000000 INFO @ Wed, 08 Dec 2021 11:44:50: 29000000 INFO @ Wed, 08 Dec 2021 11:44:54: 25000000 INFO @ Wed, 08 Dec 2021 11:44:56: 23000000 INFO @ Wed, 08 Dec 2021 11:44:57: 30000000 INFO @ Wed, 08 Dec 2021 11:45:01: 26000000 INFO @ Wed, 08 Dec 2021 11:45:02: 24000000 INFO @ Wed, 08 Dec 2021 11:45:04: 31000000 INFO @ Wed, 08 Dec 2021 11:45:08: 27000000 INFO @ Wed, 08 Dec 2021 11:45:09: 25000000 INFO @ Wed, 08 Dec 2021 11:45:11: 32000000 INFO @ Wed, 08 Dec 2021 11:45:15: 28000000 INFO @ Wed, 08 Dec 2021 11:45:15: 26000000 INFO @ Wed, 08 Dec 2021 11:45:18: 33000000 INFO @ Wed, 08 Dec 2021 11:45:19: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 11:45:19: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 11:45:19: #1 total tags in treatment: 33049726 INFO @ Wed, 08 Dec 2021 11:45:19: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 11:45:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 11:45:19: #1 tags after filtering in treatment: 33049726 INFO @ Wed, 08 Dec 2021 11:45:19: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 11:45:19: #1 finished! INFO @ Wed, 08 Dec 2021 11:45:19: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 11:45:19: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 11:45:21: #2 number of paired peaks: 19 WARNING @ Wed, 08 Dec 2021 11:45:21: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 11:45:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 11:45:22: 27000000 INFO @ Wed, 08 Dec 2021 11:45:22: 29000000 INFO @ Wed, 08 Dec 2021 11:45:28: 28000000 INFO @ Wed, 08 Dec 2021 11:45:29: 30000000 BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 11:45:34: 29000000 INFO @ Wed, 08 Dec 2021 11:45:36: 31000000 INFO @ Wed, 08 Dec 2021 11:45:41: 30000000 INFO @ Wed, 08 Dec 2021 11:45:43: 32000000 INFO @ Wed, 08 Dec 2021 11:45:47: 31000000 INFO @ Wed, 08 Dec 2021 11:45:50: 33000000 INFO @ Wed, 08 Dec 2021 11:45:50: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 11:45:50: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 11:45:50: #1 total tags in treatment: 33049726 INFO @ Wed, 08 Dec 2021 11:45:50: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 11:45:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 11:45:51: #1 tags after filtering in treatment: 33049726 INFO @ Wed, 08 Dec 2021 11:45:51: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 11:45:51: #1 finished! INFO @ Wed, 08 Dec 2021 11:45:51: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 11:45:51: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 11:45:53: #2 number of paired peaks: 19 WARNING @ Wed, 08 Dec 2021 11:45:53: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 11:45:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 11:45:53: 32000000 INFO @ Wed, 08 Dec 2021 11:45:59: 33000000 INFO @ Wed, 08 Dec 2021 11:46:00: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 11:46:00: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 11:46:00: #1 total tags in treatment: 33049726 INFO @ Wed, 08 Dec 2021 11:46:00: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 11:46:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 11:46:00: #1 tags after filtering in treatment: 33049726 INFO @ Wed, 08 Dec 2021 11:46:00: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 11:46:00: #1 finished! INFO @ Wed, 08 Dec 2021 11:46:00: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 11:46:00: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 11:46:02: #2 number of paired peaks: 19 WARNING @ Wed, 08 Dec 2021 11:46:02: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 11:46:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879256/SRX7879256.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling