Job ID = 14157841 SRX = SRX7879251 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 41940504 spots for SRR11272866/SRR11272866.sra Written 41940504 spots for SRR11272866/SRR11272866.sra fastq に変換しました。 bowtie でマッピング中... Your job 14157973 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:18 41940504 reads; of these: 41940504 (100.00%) were unpaired; of these: 612676 (1.46%) aligned 0 times 33842688 (80.69%) aligned exactly 1 time 7485140 (17.85%) aligned >1 times 98.54% overall alignment rate Time searching: 00:10:19 Overall time: 00:10:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7441533 / 41327828 = 0.1801 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 11:31:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 11:31:43: #1 read tag files... INFO @ Wed, 08 Dec 2021 11:31:43: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 11:31:50: 1000000 INFO @ Wed, 08 Dec 2021 11:31:56: 2000000 INFO @ Wed, 08 Dec 2021 11:32:02: 3000000 INFO @ Wed, 08 Dec 2021 11:32:09: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 11:32:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 11:32:13: #1 read tag files... INFO @ Wed, 08 Dec 2021 11:32:13: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 11:32:15: 5000000 INFO @ Wed, 08 Dec 2021 11:32:19: 1000000 INFO @ Wed, 08 Dec 2021 11:32:22: 6000000 INFO @ Wed, 08 Dec 2021 11:32:25: 2000000 INFO @ Wed, 08 Dec 2021 11:32:29: 7000000 INFO @ Wed, 08 Dec 2021 11:32:31: 3000000 INFO @ Wed, 08 Dec 2021 11:32:36: 8000000 INFO @ Wed, 08 Dec 2021 11:32:38: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 11:32:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 11:32:43: #1 read tag files... INFO @ Wed, 08 Dec 2021 11:32:43: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 11:32:43: 9000000 INFO @ Wed, 08 Dec 2021 11:32:44: 5000000 INFO @ Wed, 08 Dec 2021 11:32:49: 1000000 INFO @ Wed, 08 Dec 2021 11:32:50: 10000000 INFO @ Wed, 08 Dec 2021 11:32:51: 6000000 INFO @ Wed, 08 Dec 2021 11:32:56: 2000000 INFO @ Wed, 08 Dec 2021 11:32:56: 11000000 INFO @ Wed, 08 Dec 2021 11:32:57: 7000000 INFO @ Wed, 08 Dec 2021 11:33:03: 3000000 INFO @ Wed, 08 Dec 2021 11:33:03: 12000000 INFO @ Wed, 08 Dec 2021 11:33:04: 8000000 INFO @ Wed, 08 Dec 2021 11:33:09: 4000000 INFO @ Wed, 08 Dec 2021 11:33:10: 13000000 INFO @ Wed, 08 Dec 2021 11:33:11: 9000000 INFO @ Wed, 08 Dec 2021 11:33:16: 5000000 INFO @ Wed, 08 Dec 2021 11:33:17: 14000000 INFO @ Wed, 08 Dec 2021 11:33:17: 10000000 INFO @ Wed, 08 Dec 2021 11:33:22: 6000000 INFO @ Wed, 08 Dec 2021 11:33:24: 11000000 INFO @ Wed, 08 Dec 2021 11:33:24: 15000000 INFO @ Wed, 08 Dec 2021 11:33:29: 7000000 INFO @ Wed, 08 Dec 2021 11:33:31: 12000000 INFO @ Wed, 08 Dec 2021 11:33:31: 16000000 INFO @ Wed, 08 Dec 2021 11:33:35: 8000000 INFO @ Wed, 08 Dec 2021 11:33:38: 13000000 INFO @ Wed, 08 Dec 2021 11:33:38: 17000000 INFO @ Wed, 08 Dec 2021 11:33:42: 9000000 INFO @ Wed, 08 Dec 2021 11:33:44: 14000000 INFO @ Wed, 08 Dec 2021 11:33:45: 18000000 INFO @ Wed, 08 Dec 2021 11:33:49: 10000000 INFO @ Wed, 08 Dec 2021 11:33:51: 15000000 INFO @ Wed, 08 Dec 2021 11:33:52: 19000000 INFO @ Wed, 08 Dec 2021 11:33:56: 11000000 INFO @ Wed, 08 Dec 2021 11:33:57: 16000000 INFO @ Wed, 08 Dec 2021 11:33:59: 20000000 INFO @ Wed, 08 Dec 2021 11:34:02: 12000000 INFO @ Wed, 08 Dec 2021 11:34:04: 17000000 INFO @ Wed, 08 Dec 2021 11:34:06: 21000000 INFO @ Wed, 08 Dec 2021 11:34:09: 13000000 INFO @ Wed, 08 Dec 2021 11:34:11: 18000000 INFO @ Wed, 08 Dec 2021 11:34:13: 22000000 INFO @ Wed, 08 Dec 2021 11:34:16: 14000000 INFO @ Wed, 08 Dec 2021 11:34:17: 19000000 INFO @ Wed, 08 Dec 2021 11:34:20: 23000000 INFO @ Wed, 08 Dec 2021 11:34:22: 15000000 INFO @ Wed, 08 Dec 2021 11:34:24: 20000000 INFO @ Wed, 08 Dec 2021 11:34:27: 24000000 INFO @ Wed, 08 Dec 2021 11:34:29: 16000000 INFO @ Wed, 08 Dec 2021 11:34:30: 21000000 INFO @ Wed, 08 Dec 2021 11:34:34: 25000000 INFO @ Wed, 08 Dec 2021 11:34:35: 17000000 INFO @ Wed, 08 Dec 2021 11:34:37: 22000000 INFO @ Wed, 08 Dec 2021 11:34:41: 26000000 INFO @ Wed, 08 Dec 2021 11:34:42: 18000000 INFO @ Wed, 08 Dec 2021 11:34:44: 23000000 INFO @ Wed, 08 Dec 2021 11:34:48: 27000000 INFO @ Wed, 08 Dec 2021 11:34:49: 19000000 INFO @ Wed, 08 Dec 2021 11:34:50: 24000000 INFO @ Wed, 08 Dec 2021 11:34:55: 28000000 INFO @ Wed, 08 Dec 2021 11:34:55: 20000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 11:34:57: 25000000 INFO @ Wed, 08 Dec 2021 11:35:02: 29000000 INFO @ Wed, 08 Dec 2021 11:35:02: 21000000 INFO @ Wed, 08 Dec 2021 11:35:04: 26000000 INFO @ Wed, 08 Dec 2021 11:35:09: 22000000 INFO @ Wed, 08 Dec 2021 11:35:09: 30000000 INFO @ Wed, 08 Dec 2021 11:35:10: 27000000 INFO @ Wed, 08 Dec 2021 11:35:15: 23000000 INFO @ Wed, 08 Dec 2021 11:35:16: 31000000 INFO @ Wed, 08 Dec 2021 11:35:17: 28000000 INFO @ Wed, 08 Dec 2021 11:35:22: 24000000 INFO @ Wed, 08 Dec 2021 11:35:23: 32000000 INFO @ Wed, 08 Dec 2021 11:35:24: 29000000 INFO @ Wed, 08 Dec 2021 11:35:28: 25000000 INFO @ Wed, 08 Dec 2021 11:35:30: 33000000 INFO @ Wed, 08 Dec 2021 11:35:30: 30000000 INFO @ Wed, 08 Dec 2021 11:35:35: 26000000 INFO @ Wed, 08 Dec 2021 11:35:36: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 11:35:36: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 11:35:36: #1 total tags in treatment: 33886295 INFO @ Wed, 08 Dec 2021 11:35:36: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 11:35:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 11:35:37: #1 tags after filtering in treatment: 33886295 INFO @ Wed, 08 Dec 2021 11:35:37: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 11:35:37: #1 finished! INFO @ Wed, 08 Dec 2021 11:35:37: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 11:35:37: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 11:35:37: 31000000 INFO @ Wed, 08 Dec 2021 11:35:39: #2 number of paired peaks: 33 WARNING @ Wed, 08 Dec 2021 11:35:39: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 11:35:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 11:35:41: 27000000 INFO @ Wed, 08 Dec 2021 11:35:43: 32000000 INFO @ Wed, 08 Dec 2021 11:35:47: 28000000 INFO @ Wed, 08 Dec 2021 11:35:49: 33000000 INFO @ Wed, 08 Dec 2021 11:35:53: 29000000 INFO @ Wed, 08 Dec 2021 11:35:55: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 11:35:55: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 11:35:55: #1 total tags in treatment: 33886295 INFO @ Wed, 08 Dec 2021 11:35:55: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 11:35:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 11:35:55: #1 tags after filtering in treatment: 33886295 INFO @ Wed, 08 Dec 2021 11:35:55: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 11:35:55: #1 finished! INFO @ Wed, 08 Dec 2021 11:35:55: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 11:35:55: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 11:35:57: #2 number of paired peaks: 33 WARNING @ Wed, 08 Dec 2021 11:35:57: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 11:35:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 11:35:59: 30000000 BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 11:36:05: 31000000 INFO @ Wed, 08 Dec 2021 11:36:11: 32000000 INFO @ Wed, 08 Dec 2021 11:36:16: 33000000 INFO @ Wed, 08 Dec 2021 11:36:21: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 11:36:21: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 11:36:21: #1 total tags in treatment: 33886295 INFO @ Wed, 08 Dec 2021 11:36:21: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 11:36:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 11:36:22: #1 tags after filtering in treatment: 33886295 INFO @ Wed, 08 Dec 2021 11:36:22: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 11:36:22: #1 finished! INFO @ Wed, 08 Dec 2021 11:36:22: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 11:36:22: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 11:36:24: #2 number of paired peaks: 33 WARNING @ Wed, 08 Dec 2021 11:36:24: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 11:36:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879251/SRX7879251.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling