Job ID = 10165641 SRX = SRX7879249 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 39763228 spots for SRR11272864/SRR11272864.sra Written 39763228 spots for SRR11272864/SRR11272864.sra fastq に変換しました。 bowtie でマッピング中... Your job 10165922 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:50 39763228 reads; of these: 39763228 (100.00%) were unpaired; of these: 1220690 (3.07%) aligned 0 times 32310742 (81.26%) aligned exactly 1 time 6231796 (15.67%) aligned >1 times 96.93% overall alignment rate Time searching: 00:08:50 Overall time: 00:08:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6494586 / 38542538 = 0.1685 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:50:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:50:46: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:50:46: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:50:52: 1000000 INFO @ Thu, 08 Oct 2020 19:50:57: 2000000 INFO @ Thu, 08 Oct 2020 19:51:03: 3000000 INFO @ Thu, 08 Oct 2020 19:51:09: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:51:15: 5000000 INFO @ Thu, 08 Oct 2020 19:51:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:51:16: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:51:16: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:51:22: 6000000 INFO @ Thu, 08 Oct 2020 19:51:23: 1000000 INFO @ Thu, 08 Oct 2020 19:51:29: 7000000 INFO @ Thu, 08 Oct 2020 19:51:29: 2000000 INFO @ Thu, 08 Oct 2020 19:51:35: 8000000 INFO @ Thu, 08 Oct 2020 19:51:36: 3000000 INFO @ Thu, 08 Oct 2020 19:51:42: 9000000 INFO @ Thu, 08 Oct 2020 19:51:43: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:51:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:51:45: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:51:45: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:51:49: 10000000 INFO @ Thu, 08 Oct 2020 19:51:50: 5000000 INFO @ Thu, 08 Oct 2020 19:51:52: 1000000 INFO @ Thu, 08 Oct 2020 19:51:56: 11000000 INFO @ Thu, 08 Oct 2020 19:51:57: 6000000 INFO @ Thu, 08 Oct 2020 19:51:58: 2000000 INFO @ Thu, 08 Oct 2020 19:52:02: 12000000 INFO @ Thu, 08 Oct 2020 19:52:04: 7000000 INFO @ Thu, 08 Oct 2020 19:52:04: 3000000 INFO @ Thu, 08 Oct 2020 19:52:09: 13000000 INFO @ Thu, 08 Oct 2020 19:52:11: 4000000 INFO @ Thu, 08 Oct 2020 19:52:11: 8000000 INFO @ Thu, 08 Oct 2020 19:52:16: 14000000 INFO @ Thu, 08 Oct 2020 19:52:17: 5000000 INFO @ Thu, 08 Oct 2020 19:52:18: 9000000 INFO @ Thu, 08 Oct 2020 19:52:23: 15000000 INFO @ Thu, 08 Oct 2020 19:52:23: 6000000 INFO @ Thu, 08 Oct 2020 19:52:25: 10000000 INFO @ Thu, 08 Oct 2020 19:52:29: 7000000 INFO @ Thu, 08 Oct 2020 19:52:29: 16000000 INFO @ Thu, 08 Oct 2020 19:52:32: 11000000 INFO @ Thu, 08 Oct 2020 19:52:36: 8000000 INFO @ Thu, 08 Oct 2020 19:52:36: 17000000 INFO @ Thu, 08 Oct 2020 19:52:39: 12000000 INFO @ Thu, 08 Oct 2020 19:52:42: 9000000 INFO @ Thu, 08 Oct 2020 19:52:43: 18000000 INFO @ Thu, 08 Oct 2020 19:52:46: 13000000 INFO @ Thu, 08 Oct 2020 19:52:48: 10000000 INFO @ Thu, 08 Oct 2020 19:52:50: 19000000 INFO @ Thu, 08 Oct 2020 19:52:53: 14000000 INFO @ Thu, 08 Oct 2020 19:52:55: 11000000 INFO @ Thu, 08 Oct 2020 19:52:57: 20000000 INFO @ Thu, 08 Oct 2020 19:52:59: 15000000 INFO @ Thu, 08 Oct 2020 19:53:01: 12000000 INFO @ Thu, 08 Oct 2020 19:53:03: 21000000 INFO @ Thu, 08 Oct 2020 19:53:06: 16000000 INFO @ Thu, 08 Oct 2020 19:53:07: 13000000 INFO @ Thu, 08 Oct 2020 19:53:10: 22000000 INFO @ Thu, 08 Oct 2020 19:53:13: 17000000 INFO @ Thu, 08 Oct 2020 19:53:13: 14000000 INFO @ Thu, 08 Oct 2020 19:53:17: 23000000 INFO @ Thu, 08 Oct 2020 19:53:20: 15000000 INFO @ Thu, 08 Oct 2020 19:53:20: 18000000 INFO @ Thu, 08 Oct 2020 19:53:24: 24000000 INFO @ Thu, 08 Oct 2020 19:53:26: 16000000 INFO @ Thu, 08 Oct 2020 19:53:27: 19000000 INFO @ Thu, 08 Oct 2020 19:53:30: 25000000 INFO @ Thu, 08 Oct 2020 19:53:32: 17000000 INFO @ Thu, 08 Oct 2020 19:53:34: 20000000 INFO @ Thu, 08 Oct 2020 19:53:37: 26000000 INFO @ Thu, 08 Oct 2020 19:53:38: 18000000 INFO @ Thu, 08 Oct 2020 19:53:41: 21000000 INFO @ Thu, 08 Oct 2020 19:53:44: 27000000 INFO @ Thu, 08 Oct 2020 19:53:44: 19000000 INFO @ Thu, 08 Oct 2020 19:53:47: 22000000 INFO @ Thu, 08 Oct 2020 19:53:50: 28000000 INFO @ Thu, 08 Oct 2020 19:53:51: 20000000 INFO @ Thu, 08 Oct 2020 19:53:54: 23000000 INFO @ Thu, 08 Oct 2020 19:53:57: 21000000 INFO @ Thu, 08 Oct 2020 19:53:57: 29000000 INFO @ Thu, 08 Oct 2020 19:54:01: 24000000 INFO @ Thu, 08 Oct 2020 19:54:03: 22000000 INFO @ Thu, 08 Oct 2020 19:54:04: 30000000 INFO @ Thu, 08 Oct 2020 19:54:08: 25000000 INFO @ Thu, 08 Oct 2020 19:54:09: 23000000 INFO @ Thu, 08 Oct 2020 19:54:11: 31000000 INFO @ Thu, 08 Oct 2020 19:54:15: 26000000 INFO @ Thu, 08 Oct 2020 19:54:15: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 19:54:18: 32000000 INFO @ Thu, 08 Oct 2020 19:54:18: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:54:18: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:54:18: #1 total tags in treatment: 32047952 INFO @ Thu, 08 Oct 2020 19:54:18: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:54:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:54:19: #1 tags after filtering in treatment: 32047952 INFO @ Thu, 08 Oct 2020 19:54:19: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:54:19: #1 finished! INFO @ Thu, 08 Oct 2020 19:54:19: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:54:19: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:54:21: #2 number of paired peaks: 64 WARNING @ Thu, 08 Oct 2020 19:54:21: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:54:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 19:54:22: 25000000 INFO @ Thu, 08 Oct 2020 19:54:22: 27000000 INFO @ Thu, 08 Oct 2020 19:54:28: 26000000 INFO @ Thu, 08 Oct 2020 19:54:29: 28000000 INFO @ Thu, 08 Oct 2020 19:54:34: 27000000 INFO @ Thu, 08 Oct 2020 19:54:37: 29000000 INFO @ Thu, 08 Oct 2020 19:54:41: 28000000 INFO @ Thu, 08 Oct 2020 19:54:44: 30000000 INFO @ Thu, 08 Oct 2020 19:54:47: 29000000 INFO @ Thu, 08 Oct 2020 19:54:51: 31000000 INFO @ Thu, 08 Oct 2020 19:54:53: 30000000 INFO @ Thu, 08 Oct 2020 19:54:59: 32000000 INFO @ Thu, 08 Oct 2020 19:54:59: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:54:59: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:54:59: #1 total tags in treatment: 32047952 INFO @ Thu, 08 Oct 2020 19:54:59: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:54:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:55:00: #1 tags after filtering in treatment: 32047952 INFO @ Thu, 08 Oct 2020 19:55:00: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:55:00: #1 finished! INFO @ Thu, 08 Oct 2020 19:55:00: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:55:00: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:55:00: 31000000 INFO @ Thu, 08 Oct 2020 19:55:02: #2 number of paired peaks: 64 WARNING @ Thu, 08 Oct 2020 19:55:02: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:55:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 19:55:06: 32000000 INFO @ Thu, 08 Oct 2020 19:55:06: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:55:06: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:55:06: #1 total tags in treatment: 32047952 INFO @ Thu, 08 Oct 2020 19:55:06: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:55:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:55:07: #1 tags after filtering in treatment: 32047952 INFO @ Thu, 08 Oct 2020 19:55:07: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:55:07: #1 finished! INFO @ Thu, 08 Oct 2020 19:55:07: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:55:07: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:55:09: #2 number of paired peaks: 64 WARNING @ Thu, 08 Oct 2020 19:55:09: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:55:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。