Job ID = 14157839 SRX = SRX7879249 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 39763228 spots for SRR11272864/SRR11272864.sra Written 39763228 spots for SRR11272864/SRR11272864.sra fastq に変換しました。 bowtie でマッピング中... Your job 14157961 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:19 39763228 reads; of these: 39763228 (100.00%) were unpaired; of these: 1220690 (3.07%) aligned 0 times 32310742 (81.26%) aligned exactly 1 time 6231796 (15.67%) aligned >1 times 96.93% overall alignment rate Time searching: 00:08:19 Overall time: 00:08:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6494586 / 38542538 = 0.1685 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 11:28:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 11:28:24: #1 read tag files... INFO @ Wed, 08 Dec 2021 11:28:24: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 11:28:29: 1000000 INFO @ Wed, 08 Dec 2021 11:28:34: 2000000 INFO @ Wed, 08 Dec 2021 11:28:39: 3000000 INFO @ Wed, 08 Dec 2021 11:28:44: 4000000 INFO @ Wed, 08 Dec 2021 11:28:49: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 11:28:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 11:28:52: #1 read tag files... INFO @ Wed, 08 Dec 2021 11:28:52: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 11:28:54: 6000000 INFO @ Wed, 08 Dec 2021 11:28:58: 1000000 INFO @ Wed, 08 Dec 2021 11:29:00: 7000000 INFO @ Wed, 08 Dec 2021 11:29:03: 2000000 INFO @ Wed, 08 Dec 2021 11:29:05: 8000000 INFO @ Wed, 08 Dec 2021 11:29:09: 3000000 INFO @ Wed, 08 Dec 2021 11:29:10: 9000000 INFO @ Wed, 08 Dec 2021 11:29:14: 4000000 INFO @ Wed, 08 Dec 2021 11:29:16: 10000000 INFO @ Wed, 08 Dec 2021 11:29:20: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 11:29:21: 11000000 INFO @ Wed, 08 Dec 2021 11:29:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 11:29:23: #1 read tag files... INFO @ Wed, 08 Dec 2021 11:29:23: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 11:29:25: 6000000 INFO @ Wed, 08 Dec 2021 11:29:27: 12000000 INFO @ Wed, 08 Dec 2021 11:29:29: 1000000 INFO @ Wed, 08 Dec 2021 11:29:31: 7000000 INFO @ Wed, 08 Dec 2021 11:29:32: 13000000 INFO @ Wed, 08 Dec 2021 11:29:36: 2000000 INFO @ Wed, 08 Dec 2021 11:29:37: 8000000 INFO @ Wed, 08 Dec 2021 11:29:38: 14000000 INFO @ Wed, 08 Dec 2021 11:29:42: 3000000 INFO @ Wed, 08 Dec 2021 11:29:43: 9000000 INFO @ Wed, 08 Dec 2021 11:29:44: 15000000 INFO @ Wed, 08 Dec 2021 11:29:48: 10000000 INFO @ Wed, 08 Dec 2021 11:29:49: 4000000 INFO @ Wed, 08 Dec 2021 11:29:50: 16000000 INFO @ Wed, 08 Dec 2021 11:29:54: 11000000 INFO @ Wed, 08 Dec 2021 11:29:55: 5000000 INFO @ Wed, 08 Dec 2021 11:29:55: 17000000 INFO @ Wed, 08 Dec 2021 11:30:00: 12000000 INFO @ Wed, 08 Dec 2021 11:30:01: 18000000 INFO @ Wed, 08 Dec 2021 11:30:02: 6000000 INFO @ Wed, 08 Dec 2021 11:30:06: 13000000 INFO @ Wed, 08 Dec 2021 11:30:07: 19000000 INFO @ Wed, 08 Dec 2021 11:30:08: 7000000 INFO @ Wed, 08 Dec 2021 11:30:11: 14000000 INFO @ Wed, 08 Dec 2021 11:30:13: 20000000 INFO @ Wed, 08 Dec 2021 11:30:14: 8000000 INFO @ Wed, 08 Dec 2021 11:30:17: 15000000 INFO @ Wed, 08 Dec 2021 11:30:18: 21000000 INFO @ Wed, 08 Dec 2021 11:30:21: 9000000 INFO @ Wed, 08 Dec 2021 11:30:23: 16000000 INFO @ Wed, 08 Dec 2021 11:30:24: 22000000 INFO @ Wed, 08 Dec 2021 11:30:27: 10000000 INFO @ Wed, 08 Dec 2021 11:30:29: 17000000 INFO @ Wed, 08 Dec 2021 11:30:30: 23000000 INFO @ Wed, 08 Dec 2021 11:30:33: 11000000 INFO @ Wed, 08 Dec 2021 11:30:35: 18000000 INFO @ Wed, 08 Dec 2021 11:30:35: 24000000 INFO @ Wed, 08 Dec 2021 11:30:39: 12000000 INFO @ Wed, 08 Dec 2021 11:30:40: 19000000 INFO @ Wed, 08 Dec 2021 11:30:41: 25000000 INFO @ Wed, 08 Dec 2021 11:30:45: 13000000 INFO @ Wed, 08 Dec 2021 11:30:46: 20000000 INFO @ Wed, 08 Dec 2021 11:30:47: 26000000 INFO @ Wed, 08 Dec 2021 11:30:52: 14000000 INFO @ Wed, 08 Dec 2021 11:30:52: 21000000 INFO @ Wed, 08 Dec 2021 11:30:53: 27000000 INFO @ Wed, 08 Dec 2021 11:30:58: 22000000 INFO @ Wed, 08 Dec 2021 11:30:58: 15000000 INFO @ Wed, 08 Dec 2021 11:30:58: 28000000 INFO @ Wed, 08 Dec 2021 11:31:03: 23000000 INFO @ Wed, 08 Dec 2021 11:31:04: 29000000 INFO @ Wed, 08 Dec 2021 11:31:04: 16000000 INFO @ Wed, 08 Dec 2021 11:31:09: 24000000 INFO @ Wed, 08 Dec 2021 11:31:10: 30000000 INFO @ Wed, 08 Dec 2021 11:31:10: 17000000 INFO @ Wed, 08 Dec 2021 11:31:15: 25000000 INFO @ Wed, 08 Dec 2021 11:31:15: 31000000 INFO @ Wed, 08 Dec 2021 11:31:17: 18000000 INFO @ Wed, 08 Dec 2021 11:31:20: 26000000 INFO @ Wed, 08 Dec 2021 11:31:21: 32000000 INFO @ Wed, 08 Dec 2021 11:31:22: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 11:31:22: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 11:31:22: #1 total tags in treatment: 32047952 INFO @ Wed, 08 Dec 2021 11:31:22: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 11:31:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 11:31:22: #1 tags after filtering in treatment: 32047952 INFO @ Wed, 08 Dec 2021 11:31:22: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 11:31:22: #1 finished! INFO @ Wed, 08 Dec 2021 11:31:22: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 11:31:22: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 11:31:23: 19000000 INFO @ Wed, 08 Dec 2021 11:31:24: #2 number of paired peaks: 64 WARNING @ Wed, 08 Dec 2021 11:31:24: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 11:31:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 11:31:26: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 11:31:29: 20000000 INFO @ Wed, 08 Dec 2021 11:31:32: 28000000 INFO @ Wed, 08 Dec 2021 11:31:35: 21000000 INFO @ Wed, 08 Dec 2021 11:31:37: 29000000 INFO @ Wed, 08 Dec 2021 11:31:41: 22000000 INFO @ Wed, 08 Dec 2021 11:31:43: 30000000 INFO @ Wed, 08 Dec 2021 11:31:48: 23000000 INFO @ Wed, 08 Dec 2021 11:31:48: 31000000 INFO @ Wed, 08 Dec 2021 11:31:54: 24000000 INFO @ Wed, 08 Dec 2021 11:31:54: 32000000 INFO @ Wed, 08 Dec 2021 11:31:54: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 11:31:54: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 11:31:54: #1 total tags in treatment: 32047952 INFO @ Wed, 08 Dec 2021 11:31:54: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 11:31:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 11:31:55: #1 tags after filtering in treatment: 32047952 INFO @ Wed, 08 Dec 2021 11:31:55: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 11:31:55: #1 finished! INFO @ Wed, 08 Dec 2021 11:31:55: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 11:31:55: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 11:31:57: #2 number of paired peaks: 64 WARNING @ Wed, 08 Dec 2021 11:31:57: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 11:31:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 11:32:00: 25000000 INFO @ Wed, 08 Dec 2021 11:32:06: 26000000 INFO @ Wed, 08 Dec 2021 11:32:12: 27000000 INFO @ Wed, 08 Dec 2021 11:32:18: 28000000 INFO @ Wed, 08 Dec 2021 11:32:23: 29000000 BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 11:32:29: 30000000 INFO @ Wed, 08 Dec 2021 11:32:35: 31000000 INFO @ Wed, 08 Dec 2021 11:32:41: 32000000 INFO @ Wed, 08 Dec 2021 11:32:41: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 11:32:41: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 11:32:41: #1 total tags in treatment: 32047952 INFO @ Wed, 08 Dec 2021 11:32:41: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 11:32:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 11:32:42: #1 tags after filtering in treatment: 32047952 INFO @ Wed, 08 Dec 2021 11:32:42: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 11:32:42: #1 finished! INFO @ Wed, 08 Dec 2021 11:32:42: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 11:32:42: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 11:32:44: #2 number of paired peaks: 64 WARNING @ Wed, 08 Dec 2021 11:32:44: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 11:32:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879249/SRX7879249.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling