Job ID = 10165639 SRX = SRX7879247 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 46495373 spots for SRR11272862/SRR11272862.sra Written 46495373 spots for SRR11272862/SRR11272862.sra fastq に変換しました。 bowtie でマッピング中... Your job 10165945 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:51 46495373 reads; of these: 46495373 (100.00%) were unpaired; of these: 7329200 (15.76%) aligned 0 times 32700258 (70.33%) aligned exactly 1 time 6465915 (13.91%) aligned >1 times 84.24% overall alignment rate Time searching: 00:09:51 Overall time: 00:09:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6571079 / 39166173 = 0.1678 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:51:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:51:41: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:51:41: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:51:48: 1000000 INFO @ Thu, 08 Oct 2020 19:51:55: 2000000 INFO @ Thu, 08 Oct 2020 19:52:02: 3000000 INFO @ Thu, 08 Oct 2020 19:52:08: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:52:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:52:11: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:52:11: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:52:16: 5000000 INFO @ Thu, 08 Oct 2020 19:52:18: 1000000 INFO @ Thu, 08 Oct 2020 19:52:23: 6000000 INFO @ Thu, 08 Oct 2020 19:52:25: 2000000 INFO @ Thu, 08 Oct 2020 19:52:31: 7000000 INFO @ Thu, 08 Oct 2020 19:52:33: 3000000 INFO @ Thu, 08 Oct 2020 19:52:38: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:52:40: 4000000 INFO @ Thu, 08 Oct 2020 19:52:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:52:41: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:52:41: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:52:47: 9000000 INFO @ Thu, 08 Oct 2020 19:52:48: 5000000 INFO @ Thu, 08 Oct 2020 19:52:49: 1000000 INFO @ Thu, 08 Oct 2020 19:52:55: 10000000 INFO @ Thu, 08 Oct 2020 19:52:56: 6000000 INFO @ Thu, 08 Oct 2020 19:52:57: 2000000 INFO @ Thu, 08 Oct 2020 19:53:03: 11000000 INFO @ Thu, 08 Oct 2020 19:53:03: 7000000 INFO @ Thu, 08 Oct 2020 19:53:05: 3000000 INFO @ Thu, 08 Oct 2020 19:53:11: 8000000 INFO @ Thu, 08 Oct 2020 19:53:11: 12000000 INFO @ Thu, 08 Oct 2020 19:53:13: 4000000 INFO @ Thu, 08 Oct 2020 19:53:18: 9000000 INFO @ Thu, 08 Oct 2020 19:53:18: 13000000 INFO @ Thu, 08 Oct 2020 19:53:21: 5000000 INFO @ Thu, 08 Oct 2020 19:53:26: 10000000 INFO @ Thu, 08 Oct 2020 19:53:26: 14000000 INFO @ Thu, 08 Oct 2020 19:53:29: 6000000 INFO @ Thu, 08 Oct 2020 19:53:33: 11000000 INFO @ Thu, 08 Oct 2020 19:53:34: 15000000 INFO @ Thu, 08 Oct 2020 19:53:37: 7000000 INFO @ Thu, 08 Oct 2020 19:53:41: 12000000 INFO @ Thu, 08 Oct 2020 19:53:42: 16000000 INFO @ Thu, 08 Oct 2020 19:53:45: 8000000 INFO @ Thu, 08 Oct 2020 19:53:48: 13000000 INFO @ Thu, 08 Oct 2020 19:53:49: 17000000 INFO @ Thu, 08 Oct 2020 19:53:53: 9000000 INFO @ Thu, 08 Oct 2020 19:53:56: 14000000 INFO @ Thu, 08 Oct 2020 19:53:57: 18000000 INFO @ Thu, 08 Oct 2020 19:54:01: 10000000 INFO @ Thu, 08 Oct 2020 19:54:04: 15000000 INFO @ Thu, 08 Oct 2020 19:54:05: 19000000 INFO @ Thu, 08 Oct 2020 19:54:08: 11000000 INFO @ Thu, 08 Oct 2020 19:54:12: 16000000 INFO @ Thu, 08 Oct 2020 19:54:13: 20000000 INFO @ Thu, 08 Oct 2020 19:54:16: 12000000 INFO @ Thu, 08 Oct 2020 19:54:19: 17000000 INFO @ Thu, 08 Oct 2020 19:54:20: 21000000 INFO @ Thu, 08 Oct 2020 19:54:24: 13000000 INFO @ Thu, 08 Oct 2020 19:54:27: 18000000 INFO @ Thu, 08 Oct 2020 19:54:28: 22000000 INFO @ Thu, 08 Oct 2020 19:54:31: 14000000 INFO @ Thu, 08 Oct 2020 19:54:34: 19000000 INFO @ Thu, 08 Oct 2020 19:54:36: 23000000 INFO @ Thu, 08 Oct 2020 19:54:39: 15000000 INFO @ Thu, 08 Oct 2020 19:54:42: 20000000 INFO @ Thu, 08 Oct 2020 19:54:43: 24000000 INFO @ Thu, 08 Oct 2020 19:54:47: 16000000 INFO @ Thu, 08 Oct 2020 19:54:50: 21000000 INFO @ Thu, 08 Oct 2020 19:54:51: 25000000 INFO @ Thu, 08 Oct 2020 19:54:54: 17000000 INFO @ Thu, 08 Oct 2020 19:54:57: 22000000 INFO @ Thu, 08 Oct 2020 19:54:59: 26000000 INFO @ Thu, 08 Oct 2020 19:55:02: 18000000 INFO @ Thu, 08 Oct 2020 19:55:05: 23000000 INFO @ Thu, 08 Oct 2020 19:55:06: 27000000 INFO @ Thu, 08 Oct 2020 19:55:09: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 19:55:12: 24000000 INFO @ Thu, 08 Oct 2020 19:55:14: 28000000 INFO @ Thu, 08 Oct 2020 19:55:17: 20000000 INFO @ Thu, 08 Oct 2020 19:55:20: 25000000 INFO @ Thu, 08 Oct 2020 19:55:22: 29000000 INFO @ Thu, 08 Oct 2020 19:55:25: 21000000 INFO @ Thu, 08 Oct 2020 19:55:28: 26000000 INFO @ Thu, 08 Oct 2020 19:55:30: 30000000 INFO @ Thu, 08 Oct 2020 19:55:33: 22000000 INFO @ Thu, 08 Oct 2020 19:55:35: 27000000 INFO @ Thu, 08 Oct 2020 19:55:37: 31000000 INFO @ Thu, 08 Oct 2020 19:55:41: 23000000 INFO @ Thu, 08 Oct 2020 19:55:43: 28000000 INFO @ Thu, 08 Oct 2020 19:55:45: 32000000 INFO @ Thu, 08 Oct 2020 19:55:49: 24000000 INFO @ Thu, 08 Oct 2020 19:55:50: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:55:50: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:55:50: #1 total tags in treatment: 32595094 INFO @ Thu, 08 Oct 2020 19:55:50: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:55:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:55:50: 29000000 INFO @ Thu, 08 Oct 2020 19:55:50: #1 tags after filtering in treatment: 32595094 INFO @ Thu, 08 Oct 2020 19:55:50: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:55:50: #1 finished! INFO @ Thu, 08 Oct 2020 19:55:50: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:55:50: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:55:52: #2 number of paired peaks: 73 WARNING @ Thu, 08 Oct 2020 19:55:52: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:55:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 19:55:56: 25000000 INFO @ Thu, 08 Oct 2020 19:55:58: 30000000 INFO @ Thu, 08 Oct 2020 19:56:04: 26000000 INFO @ Thu, 08 Oct 2020 19:56:05: 31000000 INFO @ Thu, 08 Oct 2020 19:56:12: 27000000 BigWig に変換しました。 INFO @ Thu, 08 Oct 2020 19:56:12: 32000000 INFO @ Thu, 08 Oct 2020 19:56:17: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:56:17: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:56:17: #1 total tags in treatment: 32595094 INFO @ Thu, 08 Oct 2020 19:56:17: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:56:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:56:17: #1 tags after filtering in treatment: 32595094 INFO @ Thu, 08 Oct 2020 19:56:17: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:56:17: #1 finished! INFO @ Thu, 08 Oct 2020 19:56:17: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:56:17: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:56:19: #2 number of paired peaks: 73 WARNING @ Thu, 08 Oct 2020 19:56:19: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:56:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 19:56:20: 28000000 INFO @ Thu, 08 Oct 2020 19:56:27: 29000000 INFO @ Thu, 08 Oct 2020 19:56:34: 30000000 INFO @ Thu, 08 Oct 2020 19:56:41: 31000000 INFO @ Thu, 08 Oct 2020 19:56:48: 32000000 INFO @ Thu, 08 Oct 2020 19:56:52: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:56:52: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:56:52: #1 total tags in treatment: 32595094 INFO @ Thu, 08 Oct 2020 19:56:52: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:56:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:56:53: #1 tags after filtering in treatment: 32595094 INFO @ Thu, 08 Oct 2020 19:56:53: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:56:53: #1 finished! INFO @ Thu, 08 Oct 2020 19:56:53: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:56:53: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:56:55: #2 number of paired peaks: 73 WARNING @ Thu, 08 Oct 2020 19:56:55: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:56:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling