Job ID = 14157837 SRX = SRX7879247 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 46495373 spots for SRR11272862/SRR11272862.sra Written 46495373 spots for SRR11272862/SRR11272862.sra fastq に変換しました。 bowtie でマッピング中... Your job 14157964 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:10 46495373 reads; of these: 46495373 (100.00%) were unpaired; of these: 7329200 (15.76%) aligned 0 times 32700258 (70.33%) aligned exactly 1 time 6465915 (13.91%) aligned >1 times 84.24% overall alignment rate Time searching: 00:09:10 Overall time: 00:09:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6571079 / 39166173 = 0.1678 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 11:29:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 11:29:44: #1 read tag files... INFO @ Wed, 08 Dec 2021 11:29:44: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 11:29:51: 1000000 INFO @ Wed, 08 Dec 2021 11:29:58: 2000000 INFO @ Wed, 08 Dec 2021 11:30:05: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 11:30:12: 4000000 INFO @ Wed, 08 Dec 2021 11:30:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 11:30:13: #1 read tag files... INFO @ Wed, 08 Dec 2021 11:30:13: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 11:30:19: 5000000 INFO @ Wed, 08 Dec 2021 11:30:21: 1000000 INFO @ Wed, 08 Dec 2021 11:30:27: 6000000 INFO @ Wed, 08 Dec 2021 11:30:30: 2000000 INFO @ Wed, 08 Dec 2021 11:30:34: 7000000 INFO @ Wed, 08 Dec 2021 11:30:37: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 11:30:42: 8000000 INFO @ Wed, 08 Dec 2021 11:30:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 11:30:43: #1 read tag files... INFO @ Wed, 08 Dec 2021 11:30:43: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 11:30:46: 4000000 INFO @ Wed, 08 Dec 2021 11:30:50: 9000000 INFO @ Wed, 08 Dec 2021 11:30:51: 1000000 INFO @ Wed, 08 Dec 2021 11:30:53: 5000000 INFO @ Wed, 08 Dec 2021 11:30:58: 2000000 INFO @ Wed, 08 Dec 2021 11:30:58: 10000000 INFO @ Wed, 08 Dec 2021 11:31:01: 6000000 INFO @ Wed, 08 Dec 2021 11:31:05: 3000000 INFO @ Wed, 08 Dec 2021 11:31:06: 11000000 INFO @ Wed, 08 Dec 2021 11:31:09: 7000000 INFO @ Wed, 08 Dec 2021 11:31:13: 4000000 INFO @ Wed, 08 Dec 2021 11:31:14: 12000000 INFO @ Wed, 08 Dec 2021 11:31:17: 8000000 INFO @ Wed, 08 Dec 2021 11:31:21: 5000000 INFO @ Wed, 08 Dec 2021 11:31:22: 13000000 INFO @ Wed, 08 Dec 2021 11:31:25: 9000000 INFO @ Wed, 08 Dec 2021 11:31:28: 6000000 INFO @ Wed, 08 Dec 2021 11:31:30: 14000000 INFO @ Wed, 08 Dec 2021 11:31:33: 10000000 INFO @ Wed, 08 Dec 2021 11:31:36: 7000000 INFO @ Wed, 08 Dec 2021 11:31:38: 15000000 INFO @ Wed, 08 Dec 2021 11:31:41: 11000000 INFO @ Wed, 08 Dec 2021 11:31:43: 8000000 INFO @ Wed, 08 Dec 2021 11:31:46: 16000000 INFO @ Wed, 08 Dec 2021 11:31:49: 12000000 INFO @ Wed, 08 Dec 2021 11:31:50: 9000000 INFO @ Wed, 08 Dec 2021 11:31:54: 17000000 INFO @ Wed, 08 Dec 2021 11:31:57: 13000000 INFO @ Wed, 08 Dec 2021 11:31:58: 10000000 INFO @ Wed, 08 Dec 2021 11:32:02: 18000000 INFO @ Wed, 08 Dec 2021 11:32:05: 14000000 INFO @ Wed, 08 Dec 2021 11:32:05: 11000000 INFO @ Wed, 08 Dec 2021 11:32:10: 19000000 INFO @ Wed, 08 Dec 2021 11:32:12: 15000000 INFO @ Wed, 08 Dec 2021 11:32:13: 12000000 INFO @ Wed, 08 Dec 2021 11:32:19: 20000000 INFO @ Wed, 08 Dec 2021 11:32:20: 13000000 INFO @ Wed, 08 Dec 2021 11:32:20: 16000000 INFO @ Wed, 08 Dec 2021 11:32:27: 21000000 INFO @ Wed, 08 Dec 2021 11:32:28: 14000000 INFO @ Wed, 08 Dec 2021 11:32:28: 17000000 INFO @ Wed, 08 Dec 2021 11:32:35: 15000000 INFO @ Wed, 08 Dec 2021 11:32:36: 22000000 INFO @ Wed, 08 Dec 2021 11:32:36: 18000000 INFO @ Wed, 08 Dec 2021 11:32:42: 16000000 INFO @ Wed, 08 Dec 2021 11:32:44: 23000000 INFO @ Wed, 08 Dec 2021 11:32:44: 19000000 INFO @ Wed, 08 Dec 2021 11:32:50: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 11:32:52: 24000000 INFO @ Wed, 08 Dec 2021 11:32:53: 20000000 INFO @ Wed, 08 Dec 2021 11:32:57: 18000000 INFO @ Wed, 08 Dec 2021 11:33:01: 25000000 INFO @ Wed, 08 Dec 2021 11:33:01: 21000000 INFO @ Wed, 08 Dec 2021 11:33:05: 19000000 INFO @ Wed, 08 Dec 2021 11:33:09: 26000000 INFO @ Wed, 08 Dec 2021 11:33:09: 22000000 INFO @ Wed, 08 Dec 2021 11:33:12: 20000000 INFO @ Wed, 08 Dec 2021 11:33:17: 27000000 INFO @ Wed, 08 Dec 2021 11:33:17: 23000000 INFO @ Wed, 08 Dec 2021 11:33:19: 21000000 INFO @ Wed, 08 Dec 2021 11:33:25: 28000000 INFO @ Wed, 08 Dec 2021 11:33:26: 24000000 INFO @ Wed, 08 Dec 2021 11:33:27: 22000000 INFO @ Wed, 08 Dec 2021 11:33:33: 29000000 INFO @ Wed, 08 Dec 2021 11:33:34: 25000000 INFO @ Wed, 08 Dec 2021 11:33:34: 23000000 INFO @ Wed, 08 Dec 2021 11:33:42: 30000000 INFO @ Wed, 08 Dec 2021 11:33:42: 24000000 INFO @ Wed, 08 Dec 2021 11:33:42: 26000000 INFO @ Wed, 08 Dec 2021 11:33:49: 25000000 INFO @ Wed, 08 Dec 2021 11:33:50: 31000000 INFO @ Wed, 08 Dec 2021 11:33:50: 27000000 BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 11:33:56: 26000000 INFO @ Wed, 08 Dec 2021 11:33:58: 32000000 INFO @ Wed, 08 Dec 2021 11:33:59: 28000000 INFO @ Wed, 08 Dec 2021 11:34:03: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 11:34:03: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 11:34:03: #1 total tags in treatment: 32595094 INFO @ Wed, 08 Dec 2021 11:34:03: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 11:34:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 11:34:03: #1 tags after filtering in treatment: 32595094 INFO @ Wed, 08 Dec 2021 11:34:03: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 11:34:03: #1 finished! INFO @ Wed, 08 Dec 2021 11:34:03: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 11:34:03: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 11:34:04: 27000000 INFO @ Wed, 08 Dec 2021 11:34:05: #2 number of paired peaks: 73 WARNING @ Wed, 08 Dec 2021 11:34:05: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 11:34:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 11:34:07: 29000000 INFO @ Wed, 08 Dec 2021 11:34:11: 28000000 INFO @ Wed, 08 Dec 2021 11:34:15: 30000000 INFO @ Wed, 08 Dec 2021 11:34:18: 29000000 INFO @ Wed, 08 Dec 2021 11:34:23: 31000000 INFO @ Wed, 08 Dec 2021 11:34:26: 30000000 INFO @ Wed, 08 Dec 2021 11:34:31: 32000000 INFO @ Wed, 08 Dec 2021 11:34:33: 31000000 INFO @ Wed, 08 Dec 2021 11:34:36: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 11:34:36: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 11:34:36: #1 total tags in treatment: 32595094 INFO @ Wed, 08 Dec 2021 11:34:36: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 11:34:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 11:34:37: #1 tags after filtering in treatment: 32595094 INFO @ Wed, 08 Dec 2021 11:34:37: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 11:34:37: #1 finished! INFO @ Wed, 08 Dec 2021 11:34:37: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 11:34:37: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 11:34:39: #2 number of paired peaks: 73 WARNING @ Wed, 08 Dec 2021 11:34:39: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 11:34:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 11:34:40: 32000000 INFO @ Wed, 08 Dec 2021 11:34:44: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 11:34:44: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 11:34:44: #1 total tags in treatment: 32595094 INFO @ Wed, 08 Dec 2021 11:34:44: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 11:34:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 11:34:44: #1 tags after filtering in treatment: 32595094 INFO @ Wed, 08 Dec 2021 11:34:44: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 11:34:44: #1 finished! INFO @ Wed, 08 Dec 2021 11:34:44: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 11:34:44: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 11:34:46: #2 number of paired peaks: 73 WARNING @ Wed, 08 Dec 2021 11:34:46: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 11:34:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879247/SRX7879247.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling