Job ID = 6626363 SRX = SRX7262203 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 2735292 spots for SRR10581826/SRR10581826.sra Written 2735292 spots for SRR10581826/SRR10581826.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626386 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:42 2735292 reads; of these: 2735292 (100.00%) were unpaired; of these: 199593 (7.30%) aligned 0 times 2113391 (77.26%) aligned exactly 1 time 422308 (15.44%) aligned >1 times 92.70% overall alignment rate Time searching: 00:00:42 Overall time: 00:00:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 86807 / 2535699 = 0.0342 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 06:57:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7262203/SRX7262203.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7262203/SRX7262203.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7262203/SRX7262203.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7262203/SRX7262203.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 06:57:55: #1 read tag files... INFO @ Tue, 14 Jul 2020 06:57:55: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 06:58:02: 1000000 INFO @ Tue, 14 Jul 2020 06:58:08: 2000000 INFO @ Tue, 14 Jul 2020 06:58:11: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 06:58:11: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 06:58:11: #1 total tags in treatment: 2448892 INFO @ Tue, 14 Jul 2020 06:58:11: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 06:58:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 06:58:11: #1 tags after filtering in treatment: 2448892 INFO @ Tue, 14 Jul 2020 06:58:11: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 06:58:11: #1 finished! INFO @ Tue, 14 Jul 2020 06:58:11: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 06:58:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 06:58:11: #2 number of paired peaks: 388 WARNING @ Tue, 14 Jul 2020 06:58:11: Fewer paired peaks (388) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 388 pairs to build model! INFO @ Tue, 14 Jul 2020 06:58:11: start model_add_line... INFO @ Tue, 14 Jul 2020 06:58:11: start X-correlation... INFO @ Tue, 14 Jul 2020 06:58:11: end of X-cor INFO @ Tue, 14 Jul 2020 06:58:11: #2 finished! INFO @ Tue, 14 Jul 2020 06:58:11: #2 predicted fragment length is 48 bps INFO @ Tue, 14 Jul 2020 06:58:11: #2 alternative fragment length(s) may be 48,466 bps INFO @ Tue, 14 Jul 2020 06:58:11: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX7262203/SRX7262203.05_model.r WARNING @ Tue, 14 Jul 2020 06:58:11: #2 Since the d (48) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 06:58:11: #2 You may need to consider one of the other alternative d(s): 48,466 WARNING @ Tue, 14 Jul 2020 06:58:11: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 06:58:11: #3 Call peaks... INFO @ Tue, 14 Jul 2020 06:58:11: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 06:58:17: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 06:58:20: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX7262203/SRX7262203.05_peaks.xls INFO @ Tue, 14 Jul 2020 06:58:20: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX7262203/SRX7262203.05_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 06:58:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX7262203/SRX7262203.05_summits.bed INFO @ Tue, 14 Jul 2020 06:58:20: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (327 records, 4 fields): 22 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 06:58:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7262203/SRX7262203.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7262203/SRX7262203.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7262203/SRX7262203.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7262203/SRX7262203.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 06:58:24: #1 read tag files... INFO @ Tue, 14 Jul 2020 06:58:24: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 06:58:31: 1000000 INFO @ Tue, 14 Jul 2020 06:58:37: 2000000 INFO @ Tue, 14 Jul 2020 06:58:41: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 06:58:41: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 06:58:41: #1 total tags in treatment: 2448892 INFO @ Tue, 14 Jul 2020 06:58:41: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 06:58:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 06:58:41: #1 tags after filtering in treatment: 2448892 INFO @ Tue, 14 Jul 2020 06:58:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 06:58:41: #1 finished! INFO @ Tue, 14 Jul 2020 06:58:41: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 06:58:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 06:58:41: #2 number of paired peaks: 388 WARNING @ Tue, 14 Jul 2020 06:58:41: Fewer paired peaks (388) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 388 pairs to build model! INFO @ Tue, 14 Jul 2020 06:58:41: start model_add_line... INFO @ Tue, 14 Jul 2020 06:58:41: start X-correlation... INFO @ Tue, 14 Jul 2020 06:58:41: end of X-cor INFO @ Tue, 14 Jul 2020 06:58:41: #2 finished! INFO @ Tue, 14 Jul 2020 06:58:41: #2 predicted fragment length is 48 bps INFO @ Tue, 14 Jul 2020 06:58:41: #2 alternative fragment length(s) may be 48,466 bps INFO @ Tue, 14 Jul 2020 06:58:41: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX7262203/SRX7262203.10_model.r WARNING @ Tue, 14 Jul 2020 06:58:41: #2 Since the d (48) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 06:58:41: #2 You may need to consider one of the other alternative d(s): 48,466 WARNING @ Tue, 14 Jul 2020 06:58:41: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 06:58:41: #3 Call peaks... INFO @ Tue, 14 Jul 2020 06:58:41: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 06:58:47: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 06:58:49: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX7262203/SRX7262203.10_peaks.xls INFO @ Tue, 14 Jul 2020 06:58:49: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX7262203/SRX7262203.10_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 06:58:49: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX7262203/SRX7262203.10_summits.bed INFO @ Tue, 14 Jul 2020 06:58:49: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (178 records, 4 fields): 19 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 06:58:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7262203/SRX7262203.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7262203/SRX7262203.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7262203/SRX7262203.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7262203/SRX7262203.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 06:58:55: #1 read tag files... INFO @ Tue, 14 Jul 2020 06:58:55: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 06:59:02: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 06:59:09: 2000000 INFO @ Tue, 14 Jul 2020 06:59:12: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 06:59:12: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 06:59:12: #1 total tags in treatment: 2448892 INFO @ Tue, 14 Jul 2020 06:59:12: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 06:59:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 06:59:12: #1 tags after filtering in treatment: 2448892 INFO @ Tue, 14 Jul 2020 06:59:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 06:59:12: #1 finished! INFO @ Tue, 14 Jul 2020 06:59:12: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 06:59:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 06:59:12: #2 number of paired peaks: 388 WARNING @ Tue, 14 Jul 2020 06:59:12: Fewer paired peaks (388) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 388 pairs to build model! INFO @ Tue, 14 Jul 2020 06:59:12: start model_add_line... INFO @ Tue, 14 Jul 2020 06:59:12: start X-correlation... INFO @ Tue, 14 Jul 2020 06:59:12: end of X-cor INFO @ Tue, 14 Jul 2020 06:59:12: #2 finished! INFO @ Tue, 14 Jul 2020 06:59:12: #2 predicted fragment length is 48 bps INFO @ Tue, 14 Jul 2020 06:59:12: #2 alternative fragment length(s) may be 48,466 bps INFO @ Tue, 14 Jul 2020 06:59:12: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX7262203/SRX7262203.20_model.r WARNING @ Tue, 14 Jul 2020 06:59:12: #2 Since the d (48) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 06:59:12: #2 You may need to consider one of the other alternative d(s): 48,466 WARNING @ Tue, 14 Jul 2020 06:59:12: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 06:59:12: #3 Call peaks... INFO @ Tue, 14 Jul 2020 06:59:12: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 06:59:18: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 06:59:21: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX7262203/SRX7262203.20_peaks.xls INFO @ Tue, 14 Jul 2020 06:59:21: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX7262203/SRX7262203.20_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 06:59:21: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX7262203/SRX7262203.20_summits.bed INFO @ Tue, 14 Jul 2020 06:59:21: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (62 records, 4 fields): 10 millis CompletedMACS2peakCalling