Job ID = 6626354 SRX = SRX7262195 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 6518497 spots for SRR10581818/SRR10581818.sra Written 6518497 spots for SRR10581818/SRR10581818.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626412 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:34 6518497 reads; of these: 6518497 (100.00%) were unpaired; of these: 679384 (10.42%) aligned 0 times 4543667 (69.70%) aligned exactly 1 time 1295446 (19.87%) aligned >1 times 89.58% overall alignment rate Time searching: 00:01:34 Overall time: 00:01:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 417314 / 5839113 = 0.0715 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 06:59:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7262195/SRX7262195.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7262195/SRX7262195.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7262195/SRX7262195.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7262195/SRX7262195.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 06:59:25: #1 read tag files... INFO @ Tue, 14 Jul 2020 06:59:25: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 06:59:30: 1000000 INFO @ Tue, 14 Jul 2020 06:59:36: 2000000 INFO @ Tue, 14 Jul 2020 06:59:42: 3000000 INFO @ Tue, 14 Jul 2020 06:59:48: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 06:59:54: 5000000 INFO @ Tue, 14 Jul 2020 06:59:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7262195/SRX7262195.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7262195/SRX7262195.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7262195/SRX7262195.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7262195/SRX7262195.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 06:59:55: #1 read tag files... INFO @ Tue, 14 Jul 2020 06:59:55: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 06:59:56: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 06:59:56: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 06:59:56: #1 total tags in treatment: 5421799 INFO @ Tue, 14 Jul 2020 06:59:56: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 06:59:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 06:59:56: #1 tags after filtering in treatment: 5421799 INFO @ Tue, 14 Jul 2020 06:59:56: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 06:59:56: #1 finished! INFO @ Tue, 14 Jul 2020 06:59:56: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 06:59:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 06:59:57: #2 number of paired peaks: 486 WARNING @ Tue, 14 Jul 2020 06:59:57: Fewer paired peaks (486) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 486 pairs to build model! INFO @ Tue, 14 Jul 2020 06:59:57: start model_add_line... INFO @ Tue, 14 Jul 2020 06:59:57: start X-correlation... INFO @ Tue, 14 Jul 2020 06:59:57: end of X-cor INFO @ Tue, 14 Jul 2020 06:59:57: #2 finished! INFO @ Tue, 14 Jul 2020 06:59:57: #2 predicted fragment length is 48 bps INFO @ Tue, 14 Jul 2020 06:59:57: #2 alternative fragment length(s) may be 4,48,499 bps INFO @ Tue, 14 Jul 2020 06:59:57: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX7262195/SRX7262195.05_model.r WARNING @ Tue, 14 Jul 2020 06:59:57: #2 Since the d (48) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 06:59:57: #2 You may need to consider one of the other alternative d(s): 4,48,499 WARNING @ Tue, 14 Jul 2020 06:59:57: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 06:59:57: #3 Call peaks... INFO @ Tue, 14 Jul 2020 06:59:57: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 07:00:00: 1000000 INFO @ Tue, 14 Jul 2020 07:00:06: 2000000 INFO @ Tue, 14 Jul 2020 07:00:09: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 07:00:11: 3000000 INFO @ Tue, 14 Jul 2020 07:00:15: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX7262195/SRX7262195.05_peaks.xls INFO @ Tue, 14 Jul 2020 07:00:15: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX7262195/SRX7262195.05_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 07:00:15: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX7262195/SRX7262195.05_summits.bed INFO @ Tue, 14 Jul 2020 07:00:15: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (569 records, 4 fields): 18 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 07:00:17: 4000000 INFO @ Tue, 14 Jul 2020 07:00:22: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:00:24: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:00:24: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:00:24: #1 total tags in treatment: 5421799 INFO @ Tue, 14 Jul 2020 07:00:24: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:00:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:00:24: #1 tags after filtering in treatment: 5421799 INFO @ Tue, 14 Jul 2020 07:00:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:00:24: #1 finished! INFO @ Tue, 14 Jul 2020 07:00:24: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:00:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:00:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7262195/SRX7262195.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7262195/SRX7262195.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7262195/SRX7262195.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7262195/SRX7262195.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:00:24: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:00:24: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:00:25: #2 number of paired peaks: 486 WARNING @ Tue, 14 Jul 2020 07:00:25: Fewer paired peaks (486) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 486 pairs to build model! INFO @ Tue, 14 Jul 2020 07:00:25: start model_add_line... INFO @ Tue, 14 Jul 2020 07:00:25: start X-correlation... INFO @ Tue, 14 Jul 2020 07:00:25: end of X-cor INFO @ Tue, 14 Jul 2020 07:00:25: #2 finished! INFO @ Tue, 14 Jul 2020 07:00:25: #2 predicted fragment length is 48 bps INFO @ Tue, 14 Jul 2020 07:00:25: #2 alternative fragment length(s) may be 4,48,499 bps INFO @ Tue, 14 Jul 2020 07:00:25: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX7262195/SRX7262195.10_model.r WARNING @ Tue, 14 Jul 2020 07:00:25: #2 Since the d (48) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 07:00:25: #2 You may need to consider one of the other alternative d(s): 4,48,499 WARNING @ Tue, 14 Jul 2020 07:00:25: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 07:00:25: #3 Call peaks... INFO @ Tue, 14 Jul 2020 07:00:25: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 07:00:31: 1000000 INFO @ Tue, 14 Jul 2020 07:00:37: 2000000 INFO @ Tue, 14 Jul 2020 07:00:37: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 07:00:43: 3000000 INFO @ Tue, 14 Jul 2020 07:00:43: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX7262195/SRX7262195.10_peaks.xls INFO @ Tue, 14 Jul 2020 07:00:43: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX7262195/SRX7262195.10_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 07:00:43: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX7262195/SRX7262195.10_summits.bed INFO @ Tue, 14 Jul 2020 07:00:43: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (359 records, 4 fields): 16 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 07:00:50: 4000000 INFO @ Tue, 14 Jul 2020 07:00:56: 5000000 INFO @ Tue, 14 Jul 2020 07:00:58: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:00:58: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:00:58: #1 total tags in treatment: 5421799 INFO @ Tue, 14 Jul 2020 07:00:58: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:00:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:00:58: #1 tags after filtering in treatment: 5421799 INFO @ Tue, 14 Jul 2020 07:00:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:00:58: #1 finished! INFO @ Tue, 14 Jul 2020 07:00:58: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:00:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:00:59: #2 number of paired peaks: 486 WARNING @ Tue, 14 Jul 2020 07:00:59: Fewer paired peaks (486) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 486 pairs to build model! INFO @ Tue, 14 Jul 2020 07:00:59: start model_add_line... INFO @ Tue, 14 Jul 2020 07:00:59: start X-correlation... INFO @ Tue, 14 Jul 2020 07:00:59: end of X-cor INFO @ Tue, 14 Jul 2020 07:00:59: #2 finished! INFO @ Tue, 14 Jul 2020 07:00:59: #2 predicted fragment length is 48 bps INFO @ Tue, 14 Jul 2020 07:00:59: #2 alternative fragment length(s) may be 4,48,499 bps INFO @ Tue, 14 Jul 2020 07:00:59: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX7262195/SRX7262195.20_model.r WARNING @ Tue, 14 Jul 2020 07:00:59: #2 Since the d (48) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 07:00:59: #2 You may need to consider one of the other alternative d(s): 4,48,499 WARNING @ Tue, 14 Jul 2020 07:00:59: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 07:00:59: #3 Call peaks... INFO @ Tue, 14 Jul 2020 07:00:59: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 07:01:10: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 07:01:16: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX7262195/SRX7262195.20_peaks.xls INFO @ Tue, 14 Jul 2020 07:01:16: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX7262195/SRX7262195.20_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 07:01:16: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX7262195/SRX7262195.20_summits.bed INFO @ Tue, 14 Jul 2020 07:01:16: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (160 records, 4 fields): 18 millis CompletedMACS2peakCalling