Job ID = 14157922 SRX = SRX6717211 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 14177019 spots for SRR9969883/SRR9969883.sra Written 14177019 spots for SRR9969883/SRR9969883.sra fastq に変換しました。 bowtie でマッピング中... Your job 14158127 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:40 14177019 reads; of these: 14177019 (100.00%) were unpaired; of these: 13062050 (92.14%) aligned 0 times 965950 (6.81%) aligned exactly 1 time 149019 (1.05%) aligned >1 times 7.86% overall alignment rate Time searching: 00:01:40 Overall time: 00:01:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 262595 / 1114969 = 0.2355 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 12:50:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6717211/SRX6717211.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6717211/SRX6717211.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6717211/SRX6717211.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6717211/SRX6717211.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 12:50:34: #1 read tag files... INFO @ Wed, 08 Dec 2021 12:50:34: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 12:50:39: #1 tag size is determined as 51 bps INFO @ Wed, 08 Dec 2021 12:50:39: #1 tag size = 51 INFO @ Wed, 08 Dec 2021 12:50:39: #1 total tags in treatment: 852374 INFO @ Wed, 08 Dec 2021 12:50:39: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 12:50:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 12:50:39: #1 tags after filtering in treatment: 852374 INFO @ Wed, 08 Dec 2021 12:50:39: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 12:50:39: #1 finished! INFO @ Wed, 08 Dec 2021 12:50:39: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 12:50:39: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 12:50:39: #2 number of paired peaks: 3314 INFO @ Wed, 08 Dec 2021 12:50:39: start model_add_line... INFO @ Wed, 08 Dec 2021 12:50:39: start X-correlation... INFO @ Wed, 08 Dec 2021 12:50:39: end of X-cor INFO @ Wed, 08 Dec 2021 12:50:39: #2 finished! INFO @ Wed, 08 Dec 2021 12:50:39: #2 predicted fragment length is 122 bps INFO @ Wed, 08 Dec 2021 12:50:39: #2 alternative fragment length(s) may be 122 bps INFO @ Wed, 08 Dec 2021 12:50:39: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6717211/SRX6717211.05_model.r INFO @ Wed, 08 Dec 2021 12:50:39: #3 Call peaks... INFO @ Wed, 08 Dec 2021 12:50:39: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 12:50:41: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 12:50:42: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6717211/SRX6717211.05_peaks.xls INFO @ Wed, 08 Dec 2021 12:50:42: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6717211/SRX6717211.05_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 12:50:42: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6717211/SRX6717211.05_summits.bed INFO @ Wed, 08 Dec 2021 12:50:42: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (4198 records, 4 fields): 5 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 12:51:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6717211/SRX6717211.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6717211/SRX6717211.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6717211/SRX6717211.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6717211/SRX6717211.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 12:51:03: #1 read tag files... INFO @ Wed, 08 Dec 2021 12:51:03: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 12:51:08: #1 tag size is determined as 51 bps INFO @ Wed, 08 Dec 2021 12:51:08: #1 tag size = 51 INFO @ Wed, 08 Dec 2021 12:51:08: #1 total tags in treatment: 852374 INFO @ Wed, 08 Dec 2021 12:51:08: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 12:51:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 12:51:08: #1 tags after filtering in treatment: 852374 INFO @ Wed, 08 Dec 2021 12:51:08: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 12:51:08: #1 finished! INFO @ Wed, 08 Dec 2021 12:51:08: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 12:51:08: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 12:51:08: #2 number of paired peaks: 3314 INFO @ Wed, 08 Dec 2021 12:51:08: start model_add_line... INFO @ Wed, 08 Dec 2021 12:51:08: start X-correlation... INFO @ Wed, 08 Dec 2021 12:51:08: end of X-cor INFO @ Wed, 08 Dec 2021 12:51:08: #2 finished! INFO @ Wed, 08 Dec 2021 12:51:08: #2 predicted fragment length is 122 bps INFO @ Wed, 08 Dec 2021 12:51:08: #2 alternative fragment length(s) may be 122 bps INFO @ Wed, 08 Dec 2021 12:51:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6717211/SRX6717211.10_model.r INFO @ Wed, 08 Dec 2021 12:51:08: #3 Call peaks... INFO @ Wed, 08 Dec 2021 12:51:08: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 12:51:10: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 12:51:11: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6717211/SRX6717211.10_peaks.xls INFO @ Wed, 08 Dec 2021 12:51:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6717211/SRX6717211.10_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 12:51:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6717211/SRX6717211.10_summits.bed INFO @ Wed, 08 Dec 2021 12:51:11: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (2028 records, 4 fields): 4 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 12:51:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6717211/SRX6717211.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6717211/SRX6717211.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6717211/SRX6717211.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6717211/SRX6717211.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 12:51:33: #1 read tag files... INFO @ Wed, 08 Dec 2021 12:51:33: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 12:51:38: #1 tag size is determined as 51 bps INFO @ Wed, 08 Dec 2021 12:51:38: #1 tag size = 51 INFO @ Wed, 08 Dec 2021 12:51:38: #1 total tags in treatment: 852374 INFO @ Wed, 08 Dec 2021 12:51:38: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 12:51:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 12:51:38: #1 tags after filtering in treatment: 852374 INFO @ Wed, 08 Dec 2021 12:51:38: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 12:51:38: #1 finished! INFO @ Wed, 08 Dec 2021 12:51:38: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 12:51:38: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 12:51:38: #2 number of paired peaks: 3314 INFO @ Wed, 08 Dec 2021 12:51:38: start model_add_line... INFO @ Wed, 08 Dec 2021 12:51:38: start X-correlation... INFO @ Wed, 08 Dec 2021 12:51:38: end of X-cor INFO @ Wed, 08 Dec 2021 12:51:38: #2 finished! INFO @ Wed, 08 Dec 2021 12:51:38: #2 predicted fragment length is 122 bps INFO @ Wed, 08 Dec 2021 12:51:38: #2 alternative fragment length(s) may be 122 bps INFO @ Wed, 08 Dec 2021 12:51:38: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6717211/SRX6717211.20_model.r INFO @ Wed, 08 Dec 2021 12:51:38: #3 Call peaks... INFO @ Wed, 08 Dec 2021 12:51:38: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 12:51:40: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 12:51:41: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6717211/SRX6717211.20_peaks.xls INFO @ Wed, 08 Dec 2021 12:51:41: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6717211/SRX6717211.20_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 12:51:41: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6717211/SRX6717211.20_summits.bed INFO @ Wed, 08 Dec 2021 12:51:41: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (802 records, 4 fields): 3 millis CompletedMACS2peakCalling