Job ID = 14157908 SRX = SRX6717201 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 8115321 spots for SRR9969873/SRR9969873.sra Written 8115321 spots for SRR9969873/SRR9969873.sra fastq に変換しました。 bowtie でマッピング中... Your job 14158110 ("srTce11") has been submitted Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:18 8115321 reads; of these: 8115321 (100.00%) were unpaired; of these: 7165490 (88.30%) aligned 0 times 876175 (10.80%) aligned exactly 1 time 73656 (0.91%) aligned >1 times 11.70% overall alignment rate Time searching: 00:01:19 Overall time: 00:01:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 201878 / 949831 = 0.2125 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 12:41:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6717201/SRX6717201.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6717201/SRX6717201.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6717201/SRX6717201.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6717201/SRX6717201.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 12:41:22: #1 read tag files... INFO @ Wed, 08 Dec 2021 12:41:22: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 12:41:27: #1 tag size is determined as 76 bps INFO @ Wed, 08 Dec 2021 12:41:27: #1 tag size = 76 INFO @ Wed, 08 Dec 2021 12:41:27: #1 total tags in treatment: 747953 INFO @ Wed, 08 Dec 2021 12:41:27: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 12:41:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 12:41:27: #1 tags after filtering in treatment: 747953 INFO @ Wed, 08 Dec 2021 12:41:27: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 12:41:27: #1 finished! INFO @ Wed, 08 Dec 2021 12:41:27: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 12:41:27: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 12:41:27: #2 number of paired peaks: 4337 INFO @ Wed, 08 Dec 2021 12:41:27: start model_add_line... INFO @ Wed, 08 Dec 2021 12:41:27: start X-correlation... INFO @ Wed, 08 Dec 2021 12:41:27: end of X-cor INFO @ Wed, 08 Dec 2021 12:41:27: #2 finished! INFO @ Wed, 08 Dec 2021 12:41:27: #2 predicted fragment length is 191 bps INFO @ Wed, 08 Dec 2021 12:41:27: #2 alternative fragment length(s) may be 191 bps INFO @ Wed, 08 Dec 2021 12:41:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6717201/SRX6717201.05_model.r INFO @ Wed, 08 Dec 2021 12:41:27: #3 Call peaks... INFO @ Wed, 08 Dec 2021 12:41:27: #3 Pre-compute pvalue-qvalue table... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 12:41:29: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 12:41:30: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6717201/SRX6717201.05_peaks.xls INFO @ Wed, 08 Dec 2021 12:41:30: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6717201/SRX6717201.05_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 12:41:30: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6717201/SRX6717201.05_summits.bed INFO @ Wed, 08 Dec 2021 12:41:30: Done! INFO @ Wed, 08 Dec 2021 12:41:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6717201/SRX6717201.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6717201/SRX6717201.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6717201/SRX6717201.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6717201/SRX6717201.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 12:41:30: #1 read tag files... INFO @ Wed, 08 Dec 2021 12:41:30: #1 read treatment tags... pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (3903 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 12:41:36: #1 tag size is determined as 76 bps INFO @ Wed, 08 Dec 2021 12:41:36: #1 tag size = 76 INFO @ Wed, 08 Dec 2021 12:41:36: #1 total tags in treatment: 747953 INFO @ Wed, 08 Dec 2021 12:41:36: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 12:41:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 12:41:36: #1 tags after filtering in treatment: 747953 INFO @ Wed, 08 Dec 2021 12:41:36: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 12:41:36: #1 finished! INFO @ Wed, 08 Dec 2021 12:41:36: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 12:41:36: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 12:41:36: #2 number of paired peaks: 4337 INFO @ Wed, 08 Dec 2021 12:41:36: start model_add_line... INFO @ Wed, 08 Dec 2021 12:41:36: start X-correlation... INFO @ Wed, 08 Dec 2021 12:41:36: end of X-cor INFO @ Wed, 08 Dec 2021 12:41:36: #2 finished! INFO @ Wed, 08 Dec 2021 12:41:36: #2 predicted fragment length is 191 bps INFO @ Wed, 08 Dec 2021 12:41:36: #2 alternative fragment length(s) may be 191 bps INFO @ Wed, 08 Dec 2021 12:41:36: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6717201/SRX6717201.10_model.r INFO @ Wed, 08 Dec 2021 12:41:36: #3 Call peaks... INFO @ Wed, 08 Dec 2021 12:41:36: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 12:41:38: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 12:41:39: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6717201/SRX6717201.10_peaks.xls INFO @ Wed, 08 Dec 2021 12:41:39: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6717201/SRX6717201.10_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 12:41:39: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6717201/SRX6717201.10_summits.bed INFO @ Wed, 08 Dec 2021 12:41:39: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (1898 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 12:42:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6717201/SRX6717201.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6717201/SRX6717201.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6717201/SRX6717201.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6717201/SRX6717201.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 12:42:00: #1 read tag files... INFO @ Wed, 08 Dec 2021 12:42:00: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 12:42:06: #1 tag size is determined as 76 bps INFO @ Wed, 08 Dec 2021 12:42:06: #1 tag size = 76 INFO @ Wed, 08 Dec 2021 12:42:06: #1 total tags in treatment: 747953 INFO @ Wed, 08 Dec 2021 12:42:06: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 12:42:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 12:42:06: #1 tags after filtering in treatment: 747953 INFO @ Wed, 08 Dec 2021 12:42:06: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 12:42:06: #1 finished! INFO @ Wed, 08 Dec 2021 12:42:06: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 12:42:06: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 12:42:06: #2 number of paired peaks: 4337 INFO @ Wed, 08 Dec 2021 12:42:06: start model_add_line... INFO @ Wed, 08 Dec 2021 12:42:06: start X-correlation... INFO @ Wed, 08 Dec 2021 12:42:06: end of X-cor INFO @ Wed, 08 Dec 2021 12:42:06: #2 finished! INFO @ Wed, 08 Dec 2021 12:42:06: #2 predicted fragment length is 191 bps INFO @ Wed, 08 Dec 2021 12:42:06: #2 alternative fragment length(s) may be 191 bps INFO @ Wed, 08 Dec 2021 12:42:06: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6717201/SRX6717201.20_model.r INFO @ Wed, 08 Dec 2021 12:42:06: #3 Call peaks... INFO @ Wed, 08 Dec 2021 12:42:06: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 12:42:08: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 12:42:09: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6717201/SRX6717201.20_peaks.xls INFO @ Wed, 08 Dec 2021 12:42:09: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6717201/SRX6717201.20_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 12:42:09: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6717201/SRX6717201.20_summits.bed INFO @ Wed, 08 Dec 2021 12:42:09: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (769 records, 4 fields): 2 millis CompletedMACS2peakCalling BigWig に変換しました。