Job ID = 5720218 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-04-15T12:32:21 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:32:21 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:32:21 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:32:21 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:32:21 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:39:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:02:28 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:02:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:05:25 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:05:33 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:16:03 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:17:49 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 30,335,115 reads read : 60,670,230 reads written : 60,670,230 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:04:45 30335115 reads; of these: 30335115 (100.00%) were paired; of these: 10741663 (35.41%) aligned concordantly 0 times 14900026 (49.12%) aligned concordantly exactly 1 time 4693426 (15.47%) aligned concordantly >1 times ---- 10741663 pairs aligned concordantly 0 times; of these: 6209910 (57.81%) aligned discordantly 1 time ---- 4531753 pairs aligned 0 times concordantly or discordantly; of these: 9063506 mates make up the pairs; of these: 4528128 (49.96%) aligned 0 times 1408226 (15.54%) aligned exactly 1 time 3127152 (34.50%) aligned >1 times 92.54% overall alignment rate Time searching: 01:04:45 Overall time: 01:04:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 44 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 4508167 / 25698612 = 0.1754 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 15 Apr 2020 23:47:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619590/SRX6619590.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619590/SRX6619590.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619590/SRX6619590.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619590/SRX6619590.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 15 Apr 2020 23:47:53: #1 read tag files... INFO @ Wed, 15 Apr 2020 23:47:53: #1 read treatment tags... INFO @ Wed, 15 Apr 2020 23:48:00: 1000000 INFO @ Wed, 15 Apr 2020 23:48:08: 2000000 INFO @ Wed, 15 Apr 2020 23:48:15: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 15 Apr 2020 23:48:22: 4000000 INFO @ Wed, 15 Apr 2020 23:48:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619590/SRX6619590.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619590/SRX6619590.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619590/SRX6619590.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619590/SRX6619590.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 15 Apr 2020 23:48:23: #1 read tag files... INFO @ Wed, 15 Apr 2020 23:48:23: #1 read treatment tags... INFO @ Wed, 15 Apr 2020 23:48:29: 5000000 INFO @ Wed, 15 Apr 2020 23:48:30: 1000000 INFO @ Wed, 15 Apr 2020 23:48:35: 6000000 INFO @ Wed, 15 Apr 2020 23:48:37: 2000000 INFO @ Wed, 15 Apr 2020 23:48:42: 7000000 INFO @ Wed, 15 Apr 2020 23:48:43: 3000000 INFO @ Wed, 15 Apr 2020 23:48:48: 8000000 INFO @ Wed, 15 Apr 2020 23:48:50: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 15 Apr 2020 23:48:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619590/SRX6619590.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619590/SRX6619590.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619590/SRX6619590.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619590/SRX6619590.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 15 Apr 2020 23:48:53: #1 read tag files... INFO @ Wed, 15 Apr 2020 23:48:53: #1 read treatment tags... INFO @ Wed, 15 Apr 2020 23:48:55: 9000000 INFO @ Wed, 15 Apr 2020 23:48:57: 5000000 INFO @ Wed, 15 Apr 2020 23:49:01: 1000000 INFO @ Wed, 15 Apr 2020 23:49:02: 10000000 INFO @ Wed, 15 Apr 2020 23:49:04: 6000000 INFO @ Wed, 15 Apr 2020 23:49:09: 11000000 INFO @ Wed, 15 Apr 2020 23:49:10: 2000000 INFO @ Wed, 15 Apr 2020 23:49:12: 7000000 INFO @ Wed, 15 Apr 2020 23:49:16: 12000000 INFO @ Wed, 15 Apr 2020 23:49:18: 3000000 INFO @ Wed, 15 Apr 2020 23:49:19: 8000000 INFO @ Wed, 15 Apr 2020 23:49:24: 13000000 INFO @ Wed, 15 Apr 2020 23:49:26: 9000000 INFO @ Wed, 15 Apr 2020 23:49:27: 4000000 INFO @ Wed, 15 Apr 2020 23:49:31: 14000000 INFO @ Wed, 15 Apr 2020 23:49:33: 10000000 INFO @ Wed, 15 Apr 2020 23:49:35: 5000000 INFO @ Wed, 15 Apr 2020 23:49:38: 15000000 INFO @ Wed, 15 Apr 2020 23:49:40: 11000000 INFO @ Wed, 15 Apr 2020 23:49:43: 6000000 INFO @ Wed, 15 Apr 2020 23:49:46: 16000000 INFO @ Wed, 15 Apr 2020 23:49:48: 12000000 INFO @ Wed, 15 Apr 2020 23:49:52: 7000000 INFO @ Wed, 15 Apr 2020 23:49:53: 17000000 INFO @ Wed, 15 Apr 2020 23:49:55: 13000000 INFO @ Wed, 15 Apr 2020 23:50:00: 8000000 INFO @ Wed, 15 Apr 2020 23:50:01: 18000000 INFO @ Wed, 15 Apr 2020 23:50:02: 14000000 INFO @ Wed, 15 Apr 2020 23:50:08: 19000000 INFO @ Wed, 15 Apr 2020 23:50:09: 9000000 INFO @ Wed, 15 Apr 2020 23:50:10: 15000000 INFO @ Wed, 15 Apr 2020 23:50:15: 20000000 INFO @ Wed, 15 Apr 2020 23:50:17: 16000000 INFO @ Wed, 15 Apr 2020 23:50:17: 10000000 INFO @ Wed, 15 Apr 2020 23:50:23: 21000000 INFO @ Wed, 15 Apr 2020 23:50:24: 17000000 INFO @ Wed, 15 Apr 2020 23:50:26: 11000000 INFO @ Wed, 15 Apr 2020 23:50:30: 22000000 INFO @ Wed, 15 Apr 2020 23:50:32: 18000000 INFO @ Wed, 15 Apr 2020 23:50:35: 12000000 INFO @ Wed, 15 Apr 2020 23:50:38: 23000000 INFO @ Wed, 15 Apr 2020 23:50:39: 19000000 INFO @ Wed, 15 Apr 2020 23:50:43: 13000000 INFO @ Wed, 15 Apr 2020 23:50:45: 24000000 INFO @ Wed, 15 Apr 2020 23:50:46: 20000000 INFO @ Wed, 15 Apr 2020 23:50:52: 14000000 INFO @ Wed, 15 Apr 2020 23:50:52: 25000000 INFO @ Wed, 15 Apr 2020 23:50:54: 21000000 INFO @ Wed, 15 Apr 2020 23:51:00: 26000000 INFO @ Wed, 15 Apr 2020 23:51:01: 22000000 INFO @ Wed, 15 Apr 2020 23:51:01: 15000000 INFO @ Wed, 15 Apr 2020 23:51:07: 27000000 INFO @ Wed, 15 Apr 2020 23:51:09: 23000000 INFO @ Wed, 15 Apr 2020 23:51:10: 16000000 INFO @ Wed, 15 Apr 2020 23:51:15: 28000000 INFO @ Wed, 15 Apr 2020 23:51:16: 24000000 INFO @ Wed, 15 Apr 2020 23:51:19: 17000000 INFO @ Wed, 15 Apr 2020 23:51:23: 29000000 INFO @ Wed, 15 Apr 2020 23:51:23: 25000000 INFO @ Wed, 15 Apr 2020 23:51:27: 18000000 INFO @ Wed, 15 Apr 2020 23:51:30: 30000000 INFO @ Wed, 15 Apr 2020 23:51:31: 26000000 INFO @ Wed, 15 Apr 2020 23:51:36: 19000000 INFO @ Wed, 15 Apr 2020 23:51:37: 31000000 INFO @ Wed, 15 Apr 2020 23:51:38: 27000000 INFO @ Wed, 15 Apr 2020 23:51:44: 20000000 INFO @ Wed, 15 Apr 2020 23:51:45: 32000000 INFO @ Wed, 15 Apr 2020 23:51:46: 28000000 INFO @ Wed, 15 Apr 2020 23:51:52: 33000000 INFO @ Wed, 15 Apr 2020 23:51:52: 21000000 INFO @ Wed, 15 Apr 2020 23:51:53: 29000000 INFO @ Wed, 15 Apr 2020 23:52:00: 34000000 INFO @ Wed, 15 Apr 2020 23:52:00: 30000000 INFO @ Wed, 15 Apr 2020 23:52:01: 22000000 INFO @ Wed, 15 Apr 2020 23:52:07: 35000000 INFO @ Wed, 15 Apr 2020 23:52:08: 31000000 INFO @ Wed, 15 Apr 2020 23:52:09: 23000000 INFO @ Wed, 15 Apr 2020 23:52:14: 36000000 INFO @ Wed, 15 Apr 2020 23:52:15: 32000000 INFO @ Wed, 15 Apr 2020 23:52:18: 24000000 INFO @ Wed, 15 Apr 2020 23:52:21: 37000000 INFO @ Wed, 15 Apr 2020 23:52:22: 33000000 INFO @ Wed, 15 Apr 2020 23:52:26: 25000000 INFO @ Wed, 15 Apr 2020 23:52:29: 38000000 INFO @ Wed, 15 Apr 2020 23:52:30: 34000000 INFO @ Wed, 15 Apr 2020 23:52:35: 26000000 INFO @ Wed, 15 Apr 2020 23:52:36: 39000000 INFO @ Wed, 15 Apr 2020 23:52:37: 35000000 INFO @ Wed, 15 Apr 2020 23:52:43: 27000000 INFO @ Wed, 15 Apr 2020 23:52:43: 40000000 INFO @ Wed, 15 Apr 2020 23:52:44: 36000000 INFO @ Wed, 15 Apr 2020 23:52:51: 41000000 INFO @ Wed, 15 Apr 2020 23:52:52: 28000000 INFO @ Wed, 15 Apr 2020 23:52:52: 37000000 INFO @ Wed, 15 Apr 2020 23:52:58: 42000000 INFO @ Wed, 15 Apr 2020 23:52:59: 38000000 INFO @ Wed, 15 Apr 2020 23:53:00: 29000000 INFO @ Wed, 15 Apr 2020 23:53:05: 43000000 INFO @ Wed, 15 Apr 2020 23:53:06: 39000000 INFO @ Wed, 15 Apr 2020 23:53:09: 30000000 INFO @ Wed, 15 Apr 2020 23:53:13: 44000000 INFO @ Wed, 15 Apr 2020 23:53:14: 40000000 INFO @ Wed, 15 Apr 2020 23:53:17: 31000000 INFO @ Wed, 15 Apr 2020 23:53:20: 45000000 INFO @ Wed, 15 Apr 2020 23:53:21: 41000000 INFO @ Wed, 15 Apr 2020 23:53:25: 32000000 INFO @ Wed, 15 Apr 2020 23:53:28: 46000000 INFO @ Wed, 15 Apr 2020 23:53:28: 42000000 INFO @ Wed, 15 Apr 2020 23:53:34: 33000000 INFO @ Wed, 15 Apr 2020 23:53:35: 47000000 INFO @ Wed, 15 Apr 2020 23:53:36: 43000000 INFO @ Wed, 15 Apr 2020 23:53:36: #1 tag size is determined as 150 bps INFO @ Wed, 15 Apr 2020 23:53:36: #1 tag size = 150 INFO @ Wed, 15 Apr 2020 23:53:36: #1 total tags in treatment: 16169632 INFO @ Wed, 15 Apr 2020 23:53:36: #1 user defined the maximum tags... INFO @ Wed, 15 Apr 2020 23:53:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 15 Apr 2020 23:53:37: #1 tags after filtering in treatment: 12783879 INFO @ Wed, 15 Apr 2020 23:53:37: #1 Redundant rate of treatment: 0.21 INFO @ Wed, 15 Apr 2020 23:53:37: #1 finished! INFO @ Wed, 15 Apr 2020 23:53:37: #2 Build Peak Model... INFO @ Wed, 15 Apr 2020 23:53:37: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 15 Apr 2020 23:53:37: #2 number of paired peaks: 1682 INFO @ Wed, 15 Apr 2020 23:53:37: start model_add_line... INFO @ Wed, 15 Apr 2020 23:53:38: start X-correlation... INFO @ Wed, 15 Apr 2020 23:53:38: end of X-cor INFO @ Wed, 15 Apr 2020 23:53:38: #2 finished! INFO @ Wed, 15 Apr 2020 23:53:38: #2 predicted fragment length is 240 bps INFO @ Wed, 15 Apr 2020 23:53:38: #2 alternative fragment length(s) may be 4,240 bps INFO @ Wed, 15 Apr 2020 23:53:38: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619590/SRX6619590.05_model.r WARNING @ Wed, 15 Apr 2020 23:53:38: #2 Since the d (240) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 15 Apr 2020 23:53:38: #2 You may need to consider one of the other alternative d(s): 4,240 WARNING @ Wed, 15 Apr 2020 23:53:38: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 15 Apr 2020 23:53:38: #3 Call peaks... INFO @ Wed, 15 Apr 2020 23:53:38: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 15 Apr 2020 23:53:42: 34000000 INFO @ Wed, 15 Apr 2020 23:53:43: 44000000 INFO @ Wed, 15 Apr 2020 23:53:50: 45000000 INFO @ Wed, 15 Apr 2020 23:53:51: 35000000 INFO @ Wed, 15 Apr 2020 23:53:58: 46000000 INFO @ Wed, 15 Apr 2020 23:53:59: 36000000 INFO @ Wed, 15 Apr 2020 23:54:05: 47000000 INFO @ Wed, 15 Apr 2020 23:54:06: #1 tag size is determined as 150 bps INFO @ Wed, 15 Apr 2020 23:54:06: #1 tag size = 150 INFO @ Wed, 15 Apr 2020 23:54:06: #1 total tags in treatment: 16169632 INFO @ Wed, 15 Apr 2020 23:54:06: #1 user defined the maximum tags... INFO @ Wed, 15 Apr 2020 23:54:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 15 Apr 2020 23:54:07: #1 tags after filtering in treatment: 12783879 INFO @ Wed, 15 Apr 2020 23:54:07: #1 Redundant rate of treatment: 0.21 INFO @ Wed, 15 Apr 2020 23:54:07: #1 finished! INFO @ Wed, 15 Apr 2020 23:54:07: #2 Build Peak Model... INFO @ Wed, 15 Apr 2020 23:54:07: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 15 Apr 2020 23:54:07: 37000000 INFO @ Wed, 15 Apr 2020 23:54:08: #2 number of paired peaks: 1682 INFO @ Wed, 15 Apr 2020 23:54:08: start model_add_line... INFO @ Wed, 15 Apr 2020 23:54:08: start X-correlation... INFO @ Wed, 15 Apr 2020 23:54:08: end of X-cor INFO @ Wed, 15 Apr 2020 23:54:08: #2 finished! INFO @ Wed, 15 Apr 2020 23:54:08: #2 predicted fragment length is 240 bps INFO @ Wed, 15 Apr 2020 23:54:08: #2 alternative fragment length(s) may be 4,240 bps INFO @ Wed, 15 Apr 2020 23:54:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619590/SRX6619590.10_model.r WARNING @ Wed, 15 Apr 2020 23:54:08: #2 Since the d (240) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 15 Apr 2020 23:54:08: #2 You may need to consider one of the other alternative d(s): 4,240 WARNING @ Wed, 15 Apr 2020 23:54:08: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 15 Apr 2020 23:54:08: #3 Call peaks... INFO @ Wed, 15 Apr 2020 23:54:08: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 15 Apr 2020 23:54:12: #3 Call peaks for each chromosome... INFO @ Wed, 15 Apr 2020 23:54:15: 38000000 INFO @ Wed, 15 Apr 2020 23:54:23: 39000000 INFO @ Wed, 15 Apr 2020 23:54:26: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619590/SRX6619590.05_peaks.xls INFO @ Wed, 15 Apr 2020 23:54:26: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619590/SRX6619590.05_peaks.narrowPeak INFO @ Wed, 15 Apr 2020 23:54:26: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619590/SRX6619590.05_summits.bed INFO @ Wed, 15 Apr 2020 23:54:26: Done! pass1 - making usageList (6 chroms): 2 millis pass2 - checking and writing primary data (9196 records, 4 fields): 9 millis CompletedMACS2peakCalling INFO @ Wed, 15 Apr 2020 23:54:31: 40000000 INFO @ Wed, 15 Apr 2020 23:54:39: 41000000 INFO @ Wed, 15 Apr 2020 23:54:42: #3 Call peaks for each chromosome... INFO @ Wed, 15 Apr 2020 23:54:47: 42000000 INFO @ Wed, 15 Apr 2020 23:54:55: 43000000 INFO @ Wed, 15 Apr 2020 23:54:56: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619590/SRX6619590.10_peaks.xls INFO @ Wed, 15 Apr 2020 23:54:56: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619590/SRX6619590.10_peaks.narrowPeak INFO @ Wed, 15 Apr 2020 23:54:56: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619590/SRX6619590.10_summits.bed INFO @ Wed, 15 Apr 2020 23:54:56: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (4363 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Wed, 15 Apr 2020 23:55:03: 44000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 15 Apr 2020 23:55:11: 45000000 INFO @ Wed, 15 Apr 2020 23:55:19: 46000000 INFO @ Wed, 15 Apr 2020 23:55:27: 47000000 INFO @ Wed, 15 Apr 2020 23:55:28: #1 tag size is determined as 150 bps INFO @ Wed, 15 Apr 2020 23:55:28: #1 tag size = 150 INFO @ Wed, 15 Apr 2020 23:55:28: #1 total tags in treatment: 16169632 INFO @ Wed, 15 Apr 2020 23:55:28: #1 user defined the maximum tags... INFO @ Wed, 15 Apr 2020 23:55:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 15 Apr 2020 23:55:28: #1 tags after filtering in treatment: 12783879 INFO @ Wed, 15 Apr 2020 23:55:28: #1 Redundant rate of treatment: 0.21 INFO @ Wed, 15 Apr 2020 23:55:28: #1 finished! INFO @ Wed, 15 Apr 2020 23:55:28: #2 Build Peak Model... INFO @ Wed, 15 Apr 2020 23:55:28: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 15 Apr 2020 23:55:29: #2 number of paired peaks: 1682 INFO @ Wed, 15 Apr 2020 23:55:29: start model_add_line... INFO @ Wed, 15 Apr 2020 23:55:29: start X-correlation... INFO @ Wed, 15 Apr 2020 23:55:29: end of X-cor INFO @ Wed, 15 Apr 2020 23:55:29: #2 finished! INFO @ Wed, 15 Apr 2020 23:55:29: #2 predicted fragment length is 240 bps INFO @ Wed, 15 Apr 2020 23:55:29: #2 alternative fragment length(s) may be 4,240 bps INFO @ Wed, 15 Apr 2020 23:55:29: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619590/SRX6619590.20_model.r WARNING @ Wed, 15 Apr 2020 23:55:29: #2 Since the d (240) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 15 Apr 2020 23:55:29: #2 You may need to consider one of the other alternative d(s): 4,240 WARNING @ Wed, 15 Apr 2020 23:55:29: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 15 Apr 2020 23:55:29: #3 Call peaks... INFO @ Wed, 15 Apr 2020 23:55:29: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Wed, 15 Apr 2020 23:56:04: #3 Call peaks for each chromosome... INFO @ Wed, 15 Apr 2020 23:56:17: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619590/SRX6619590.20_peaks.xls INFO @ Wed, 15 Apr 2020 23:56:17: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619590/SRX6619590.20_peaks.narrowPeak INFO @ Wed, 15 Apr 2020 23:56:17: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619590/SRX6619590.20_summits.bed INFO @ Wed, 15 Apr 2020 23:56:17: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (1547 records, 4 fields): 2 millis CompletedMACS2peakCalling