Job ID = 5720210 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-04-15T13:01:54 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:05:25 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:07:05 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:09:27 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:15:39 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:23:09 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:33:14 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:35:33 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:43:24 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:44:37 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 41,642,076 reads read : 83,284,152 reads written : 83,284,152 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:10:09 41642076 reads; of these: 41642076 (100.00%) were paired; of these: 25442308 (61.10%) aligned concordantly 0 times 13340754 (32.04%) aligned concordantly exactly 1 time 2859014 (6.87%) aligned concordantly >1 times ---- 25442308 pairs aligned concordantly 0 times; of these: 13068881 (51.37%) aligned discordantly 1 time ---- 12373427 pairs aligned 0 times concordantly or discordantly; of these: 24746854 mates make up the pairs; of these: 21313638 (86.13%) aligned 0 times 1142313 (4.62%) aligned exactly 1 time 2290903 (9.26%) aligned >1 times 74.41% overall alignment rate Time searching: 01:10:09 Overall time: 01:10:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 48 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 4266655 / 28689315 = 0.1487 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:26:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619582/SRX6619582.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619582/SRX6619582.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619582/SRX6619582.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619582/SRX6619582.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:26:19: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:26:19: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:26:27: 1000000 INFO @ Thu, 16 Apr 2020 00:26:34: 2000000 INFO @ Thu, 16 Apr 2020 00:26:41: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:26:48: 4000000 INFO @ Thu, 16 Apr 2020 00:26:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619582/SRX6619582.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619582/SRX6619582.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619582/SRX6619582.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619582/SRX6619582.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:26:49: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:26:49: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:26:56: 5000000 INFO @ Thu, 16 Apr 2020 00:26:57: 1000000 INFO @ Thu, 16 Apr 2020 00:27:03: 6000000 INFO @ Thu, 16 Apr 2020 00:27:04: 2000000 INFO @ Thu, 16 Apr 2020 00:27:11: 7000000 INFO @ Thu, 16 Apr 2020 00:27:12: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:27:18: 8000000 INFO @ Thu, 16 Apr 2020 00:27:19: 4000000 INFO @ Thu, 16 Apr 2020 00:27:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619582/SRX6619582.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619582/SRX6619582.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619582/SRX6619582.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619582/SRX6619582.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:27:19: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:27:19: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:27:27: 9000000 INFO @ Thu, 16 Apr 2020 00:27:27: 5000000 INFO @ Thu, 16 Apr 2020 00:27:28: 1000000 INFO @ Thu, 16 Apr 2020 00:27:36: 10000000 INFO @ Thu, 16 Apr 2020 00:27:36: 6000000 INFO @ Thu, 16 Apr 2020 00:27:36: 2000000 INFO @ Thu, 16 Apr 2020 00:27:44: 11000000 INFO @ Thu, 16 Apr 2020 00:27:45: 3000000 INFO @ Thu, 16 Apr 2020 00:27:45: 7000000 INFO @ Thu, 16 Apr 2020 00:27:53: 4000000 INFO @ Thu, 16 Apr 2020 00:27:53: 12000000 INFO @ Thu, 16 Apr 2020 00:27:53: 8000000 INFO @ Thu, 16 Apr 2020 00:28:01: 5000000 INFO @ Thu, 16 Apr 2020 00:28:01: 13000000 INFO @ Thu, 16 Apr 2020 00:28:02: 9000000 INFO @ Thu, 16 Apr 2020 00:28:09: 6000000 INFO @ Thu, 16 Apr 2020 00:28:10: 14000000 INFO @ Thu, 16 Apr 2020 00:28:10: 10000000 INFO @ Thu, 16 Apr 2020 00:28:18: 7000000 INFO @ Thu, 16 Apr 2020 00:28:18: 15000000 INFO @ Thu, 16 Apr 2020 00:28:19: 11000000 INFO @ Thu, 16 Apr 2020 00:28:26: 8000000 INFO @ Thu, 16 Apr 2020 00:28:27: 16000000 INFO @ Thu, 16 Apr 2020 00:28:27: 12000000 INFO @ Thu, 16 Apr 2020 00:28:34: 9000000 INFO @ Thu, 16 Apr 2020 00:28:35: 17000000 INFO @ Thu, 16 Apr 2020 00:28:35: 13000000 INFO @ Thu, 16 Apr 2020 00:28:42: 10000000 INFO @ Thu, 16 Apr 2020 00:28:43: 18000000 INFO @ Thu, 16 Apr 2020 00:28:44: 14000000 INFO @ Thu, 16 Apr 2020 00:28:50: 11000000 INFO @ Thu, 16 Apr 2020 00:28:52: 19000000 INFO @ Thu, 16 Apr 2020 00:28:52: 15000000 INFO @ Thu, 16 Apr 2020 00:28:59: 12000000 INFO @ Thu, 16 Apr 2020 00:29:00: 20000000 INFO @ Thu, 16 Apr 2020 00:29:00: 16000000 INFO @ Thu, 16 Apr 2020 00:29:07: 13000000 INFO @ Thu, 16 Apr 2020 00:29:08: 21000000 INFO @ Thu, 16 Apr 2020 00:29:08: 17000000 INFO @ Thu, 16 Apr 2020 00:29:15: 14000000 INFO @ Thu, 16 Apr 2020 00:29:16: 22000000 INFO @ Thu, 16 Apr 2020 00:29:17: 18000000 INFO @ Thu, 16 Apr 2020 00:29:23: 15000000 INFO @ Thu, 16 Apr 2020 00:29:25: 23000000 INFO @ Thu, 16 Apr 2020 00:29:25: 19000000 INFO @ Thu, 16 Apr 2020 00:29:31: 16000000 INFO @ Thu, 16 Apr 2020 00:29:33: 24000000 INFO @ Thu, 16 Apr 2020 00:29:33: 20000000 INFO @ Thu, 16 Apr 2020 00:29:39: 17000000 INFO @ Thu, 16 Apr 2020 00:29:41: 25000000 INFO @ Thu, 16 Apr 2020 00:29:42: 21000000 INFO @ Thu, 16 Apr 2020 00:29:47: 18000000 INFO @ Thu, 16 Apr 2020 00:29:49: 26000000 INFO @ Thu, 16 Apr 2020 00:29:50: 22000000 INFO @ Thu, 16 Apr 2020 00:29:55: 19000000 INFO @ Thu, 16 Apr 2020 00:29:57: 27000000 INFO @ Thu, 16 Apr 2020 00:29:58: 23000000 INFO @ Thu, 16 Apr 2020 00:30:03: 20000000 INFO @ Thu, 16 Apr 2020 00:30:06: 28000000 INFO @ Thu, 16 Apr 2020 00:30:06: 24000000 INFO @ Thu, 16 Apr 2020 00:30:11: 21000000 INFO @ Thu, 16 Apr 2020 00:30:14: 29000000 INFO @ Thu, 16 Apr 2020 00:30:14: 25000000 INFO @ Thu, 16 Apr 2020 00:30:20: 22000000 INFO @ Thu, 16 Apr 2020 00:30:21: 30000000 INFO @ Thu, 16 Apr 2020 00:30:22: 26000000 INFO @ Thu, 16 Apr 2020 00:30:28: 23000000 INFO @ Thu, 16 Apr 2020 00:30:29: 31000000 INFO @ Thu, 16 Apr 2020 00:30:30: 27000000 INFO @ Thu, 16 Apr 2020 00:30:36: 24000000 INFO @ Thu, 16 Apr 2020 00:30:38: 32000000 INFO @ Thu, 16 Apr 2020 00:30:38: 28000000 INFO @ Thu, 16 Apr 2020 00:30:44: 25000000 INFO @ Thu, 16 Apr 2020 00:30:46: 33000000 INFO @ Thu, 16 Apr 2020 00:30:46: 29000000 INFO @ Thu, 16 Apr 2020 00:30:52: 26000000 INFO @ Thu, 16 Apr 2020 00:30:54: 30000000 INFO @ Thu, 16 Apr 2020 00:30:54: 34000000 INFO @ Thu, 16 Apr 2020 00:31:00: 27000000 INFO @ Thu, 16 Apr 2020 00:31:02: 31000000 INFO @ Thu, 16 Apr 2020 00:31:02: 35000000 INFO @ Thu, 16 Apr 2020 00:31:08: 28000000 INFO @ Thu, 16 Apr 2020 00:31:10: 32000000 INFO @ Thu, 16 Apr 2020 00:31:10: 36000000 INFO @ Thu, 16 Apr 2020 00:31:16: 29000000 INFO @ Thu, 16 Apr 2020 00:31:18: 33000000 INFO @ Thu, 16 Apr 2020 00:31:18: 37000000 INFO @ Thu, 16 Apr 2020 00:31:24: 30000000 INFO @ Thu, 16 Apr 2020 00:31:26: 34000000 INFO @ Thu, 16 Apr 2020 00:31:26: 38000000 INFO @ Thu, 16 Apr 2020 00:31:32: 31000000 INFO @ Thu, 16 Apr 2020 00:31:34: 39000000 INFO @ Thu, 16 Apr 2020 00:31:35: 35000000 INFO @ Thu, 16 Apr 2020 00:31:41: 32000000 INFO @ Thu, 16 Apr 2020 00:31:43: 40000000 INFO @ Thu, 16 Apr 2020 00:31:43: 36000000 INFO @ Thu, 16 Apr 2020 00:31:50: 33000000 INFO @ Thu, 16 Apr 2020 00:31:52: 37000000 INFO @ Thu, 16 Apr 2020 00:31:52: 41000000 INFO @ Thu, 16 Apr 2020 00:31:59: 34000000 INFO @ Thu, 16 Apr 2020 00:32:01: 42000000 INFO @ Thu, 16 Apr 2020 00:32:01: 38000000 INFO @ Thu, 16 Apr 2020 00:32:08: 35000000 INFO @ Thu, 16 Apr 2020 00:32:09: 43000000 INFO @ Thu, 16 Apr 2020 00:32:10: 39000000 INFO @ Thu, 16 Apr 2020 00:32:17: 36000000 INFO @ Thu, 16 Apr 2020 00:32:17: 44000000 INFO @ Thu, 16 Apr 2020 00:32:18: 40000000 INFO @ Thu, 16 Apr 2020 00:32:25: 37000000 INFO @ Thu, 16 Apr 2020 00:32:25: 45000000 INFO @ Thu, 16 Apr 2020 00:32:27: 41000000 INFO @ Thu, 16 Apr 2020 00:32:34: 38000000 INFO @ Thu, 16 Apr 2020 00:32:34: 46000000 INFO @ Thu, 16 Apr 2020 00:32:35: 42000000 INFO @ Thu, 16 Apr 2020 00:32:42: 47000000 INFO @ Thu, 16 Apr 2020 00:32:42: 39000000 INFO @ Thu, 16 Apr 2020 00:32:44: 43000000 INFO @ Thu, 16 Apr 2020 00:32:50: 48000000 INFO @ Thu, 16 Apr 2020 00:32:50: 40000000 INFO @ Thu, 16 Apr 2020 00:32:52: 44000000 INFO @ Thu, 16 Apr 2020 00:32:58: 49000000 INFO @ Thu, 16 Apr 2020 00:32:58: 41000000 INFO @ Thu, 16 Apr 2020 00:33:00: 45000000 INFO @ Thu, 16 Apr 2020 00:33:06: 50000000 INFO @ Thu, 16 Apr 2020 00:33:07: 42000000 INFO @ Thu, 16 Apr 2020 00:33:08: 46000000 INFO @ Thu, 16 Apr 2020 00:33:14: 51000000 INFO @ Thu, 16 Apr 2020 00:33:15: 43000000 INFO @ Thu, 16 Apr 2020 00:33:16: 47000000 INFO @ Thu, 16 Apr 2020 00:33:22: 52000000 INFO @ Thu, 16 Apr 2020 00:33:23: 44000000 INFO @ Thu, 16 Apr 2020 00:33:25: 48000000 INFO @ Thu, 16 Apr 2020 00:33:30: 53000000 INFO @ Thu, 16 Apr 2020 00:33:31: 45000000 INFO @ Thu, 16 Apr 2020 00:33:33: 49000000 INFO @ Thu, 16 Apr 2020 00:33:33: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:33:33: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:33:33: #1 total tags in treatment: 13579575 INFO @ Thu, 16 Apr 2020 00:33:33: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:33:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:33:33: #1 tags after filtering in treatment: 11370108 INFO @ Thu, 16 Apr 2020 00:33:33: #1 Redundant rate of treatment: 0.16 INFO @ Thu, 16 Apr 2020 00:33:33: #1 finished! INFO @ Thu, 16 Apr 2020 00:33:33: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:33:33: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:33:34: #2 number of paired peaks: 279 WARNING @ Thu, 16 Apr 2020 00:33:34: Fewer paired peaks (279) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 279 pairs to build model! INFO @ Thu, 16 Apr 2020 00:33:34: start model_add_line... INFO @ Thu, 16 Apr 2020 00:33:34: start X-correlation... INFO @ Thu, 16 Apr 2020 00:33:34: end of X-cor INFO @ Thu, 16 Apr 2020 00:33:34: #2 finished! INFO @ Thu, 16 Apr 2020 00:33:34: #2 predicted fragment length is 213 bps INFO @ Thu, 16 Apr 2020 00:33:34: #2 alternative fragment length(s) may be 213 bps INFO @ Thu, 16 Apr 2020 00:33:34: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619582/SRX6619582.05_model.r WARNING @ Thu, 16 Apr 2020 00:33:34: #2 Since the d (213) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:33:34: #2 You may need to consider one of the other alternative d(s): 213 WARNING @ Thu, 16 Apr 2020 00:33:34: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:33:34: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:33:34: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:33:40: 46000000 INFO @ Thu, 16 Apr 2020 00:33:41: 50000000 INFO @ Thu, 16 Apr 2020 00:33:48: 47000000 INFO @ Thu, 16 Apr 2020 00:33:49: 51000000 INFO @ Thu, 16 Apr 2020 00:33:56: 48000000 INFO @ Thu, 16 Apr 2020 00:33:57: 52000000 INFO @ Thu, 16 Apr 2020 00:33:57: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:34:04: 49000000 INFO @ Thu, 16 Apr 2020 00:34:05: 53000000 INFO @ Thu, 16 Apr 2020 00:34:07: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619582/SRX6619582.05_peaks.xls INFO @ Thu, 16 Apr 2020 00:34:07: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619582/SRX6619582.05_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:34:07: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619582/SRX6619582.05_summits.bed INFO @ Thu, 16 Apr 2020 00:34:07: Done! INFO @ Thu, 16 Apr 2020 00:34:08: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:34:08: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:34:08: #1 total tags in treatment: 13579575 INFO @ Thu, 16 Apr 2020 00:34:08: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:34:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:34:09: #1 tags after filtering in treatment: 11370108 INFO @ Thu, 16 Apr 2020 00:34:09: #1 Redundant rate of treatment: 0.16 INFO @ Thu, 16 Apr 2020 00:34:09: #1 finished! INFO @ Thu, 16 Apr 2020 00:34:09: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:34:09: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:34:09: #2 number of paired peaks: 279 WARNING @ Thu, 16 Apr 2020 00:34:09: Fewer paired peaks (279) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 279 pairs to build model! INFO @ Thu, 16 Apr 2020 00:34:09: start model_add_line... INFO @ Thu, 16 Apr 2020 00:34:09: start X-correlation... INFO @ Thu, 16 Apr 2020 00:34:09: end of X-cor INFO @ Thu, 16 Apr 2020 00:34:09: #2 finished! INFO @ Thu, 16 Apr 2020 00:34:09: #2 predicted fragment length is 213 bps INFO @ Thu, 16 Apr 2020 00:34:09: #2 alternative fragment length(s) may be 213 bps INFO @ Thu, 16 Apr 2020 00:34:09: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619582/SRX6619582.10_model.r WARNING @ Thu, 16 Apr 2020 00:34:09: #2 Since the d (213) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:34:09: #2 You may need to consider one of the other alternative d(s): 213 WARNING @ Thu, 16 Apr 2020 00:34:09: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:34:09: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:34:09: #3 Pre-compute pvalue-qvalue table... pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (523 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:34:11: 50000000 INFO @ Thu, 16 Apr 2020 00:34:19: 51000000 INFO @ Thu, 16 Apr 2020 00:34:27: 52000000 INFO @ Thu, 16 Apr 2020 00:34:33: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:34:34: 53000000 INFO @ Thu, 16 Apr 2020 00:34:37: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:34:37: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:34:37: #1 total tags in treatment: 13579575 INFO @ Thu, 16 Apr 2020 00:34:37: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:34:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:34:37: #1 tags after filtering in treatment: 11370108 INFO @ Thu, 16 Apr 2020 00:34:37: #1 Redundant rate of treatment: 0.16 INFO @ Thu, 16 Apr 2020 00:34:37: #1 finished! INFO @ Thu, 16 Apr 2020 00:34:37: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:34:37: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:34:38: #2 number of paired peaks: 279 WARNING @ Thu, 16 Apr 2020 00:34:38: Fewer paired peaks (279) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 279 pairs to build model! INFO @ Thu, 16 Apr 2020 00:34:38: start model_add_line... INFO @ Thu, 16 Apr 2020 00:34:38: start X-correlation... INFO @ Thu, 16 Apr 2020 00:34:38: end of X-cor INFO @ Thu, 16 Apr 2020 00:34:38: #2 finished! INFO @ Thu, 16 Apr 2020 00:34:38: #2 predicted fragment length is 213 bps INFO @ Thu, 16 Apr 2020 00:34:38: #2 alternative fragment length(s) may be 213 bps INFO @ Thu, 16 Apr 2020 00:34:38: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619582/SRX6619582.20_model.r WARNING @ Thu, 16 Apr 2020 00:34:38: #2 Since the d (213) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:34:38: #2 You may need to consider one of the other alternative d(s): 213 WARNING @ Thu, 16 Apr 2020 00:34:38: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:34:38: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:34:38: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:34:43: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619582/SRX6619582.10_peaks.xls INFO @ Thu, 16 Apr 2020 00:34:43: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619582/SRX6619582.10_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:34:43: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619582/SRX6619582.10_summits.bed INFO @ Thu, 16 Apr 2020 00:34:43: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (357 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:35:02: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:35:12: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619582/SRX6619582.20_peaks.xls INFO @ Thu, 16 Apr 2020 00:35:12: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619582/SRX6619582.20_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:35:12: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619582/SRX6619582.20_summits.bed INFO @ Thu, 16 Apr 2020 00:35:12: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (221 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。