Job ID = 5720204 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 43,788,202 reads read : 87,576,404 reads written : 87,576,404 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 02:05:25 43788202 reads; of these: 43788202 (100.00%) were paired; of these: 23576886 (53.84%) aligned concordantly 0 times 15132731 (34.56%) aligned concordantly exactly 1 time 5078585 (11.60%) aligned concordantly >1 times ---- 23576886 pairs aligned concordantly 0 times; of these: 10851327 (46.03%) aligned discordantly 1 time ---- 12725559 pairs aligned 0 times concordantly or discordantly; of these: 25451118 mates make up the pairs; of these: 21078572 (82.82%) aligned 0 times 1228550 (4.83%) aligned exactly 1 time 3143996 (12.35%) aligned >1 times 75.93% overall alignment rate Time searching: 02:05:25 Overall time: 02:05:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 52 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 5335561 / 30542322 = 0.1747 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 01:20:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619573/SRX6619573.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619573/SRX6619573.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619573/SRX6619573.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619573/SRX6619573.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 01:20:45: #1 read tag files... INFO @ Thu, 16 Apr 2020 01:20:45: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 01:20:55: 1000000 INFO @ Thu, 16 Apr 2020 01:21:05: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 01:21:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619573/SRX6619573.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619573/SRX6619573.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619573/SRX6619573.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619573/SRX6619573.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 01:21:15: #1 read tag files... INFO @ Thu, 16 Apr 2020 01:21:15: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 01:21:15: 3000000 INFO @ Thu, 16 Apr 2020 01:21:25: 1000000 INFO @ Thu, 16 Apr 2020 01:21:25: 4000000 INFO @ Thu, 16 Apr 2020 01:21:34: 2000000 INFO @ Thu, 16 Apr 2020 01:21:35: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 01:21:44: 3000000 INFO @ Thu, 16 Apr 2020 01:21:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619573/SRX6619573.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619573/SRX6619573.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619573/SRX6619573.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619573/SRX6619573.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 01:21:45: #1 read tag files... INFO @ Thu, 16 Apr 2020 01:21:45: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 01:21:46: 6000000 INFO @ Thu, 16 Apr 2020 01:21:54: 4000000 INFO @ Thu, 16 Apr 2020 01:21:55: 1000000 INFO @ Thu, 16 Apr 2020 01:21:57: 7000000 INFO @ Thu, 16 Apr 2020 01:22:04: 5000000 INFO @ Thu, 16 Apr 2020 01:22:07: 2000000 INFO @ Thu, 16 Apr 2020 01:22:08: 8000000 INFO @ Thu, 16 Apr 2020 01:22:14: 6000000 INFO @ Thu, 16 Apr 2020 01:22:18: 3000000 INFO @ Thu, 16 Apr 2020 01:22:19: 9000000 INFO @ Thu, 16 Apr 2020 01:22:23: 7000000 INFO @ Thu, 16 Apr 2020 01:22:30: 4000000 INFO @ Thu, 16 Apr 2020 01:22:31: 10000000 INFO @ Thu, 16 Apr 2020 01:22:33: 8000000 INFO @ Thu, 16 Apr 2020 01:22:42: 5000000 INFO @ Thu, 16 Apr 2020 01:22:43: 11000000 INFO @ Thu, 16 Apr 2020 01:22:43: 9000000 INFO @ Thu, 16 Apr 2020 01:22:53: 10000000 INFO @ Thu, 16 Apr 2020 01:22:54: 6000000 INFO @ Thu, 16 Apr 2020 01:22:54: 12000000 INFO @ Thu, 16 Apr 2020 01:23:03: 11000000 INFO @ Thu, 16 Apr 2020 01:23:05: 13000000 INFO @ Thu, 16 Apr 2020 01:23:06: 7000000 INFO @ Thu, 16 Apr 2020 01:23:13: 12000000 INFO @ Thu, 16 Apr 2020 01:23:16: 14000000 INFO @ Thu, 16 Apr 2020 01:23:17: 8000000 INFO @ Thu, 16 Apr 2020 01:23:22: 13000000 INFO @ Thu, 16 Apr 2020 01:23:27: 15000000 INFO @ Thu, 16 Apr 2020 01:23:28: 9000000 INFO @ Thu, 16 Apr 2020 01:23:32: 14000000 INFO @ Thu, 16 Apr 2020 01:23:38: 16000000 INFO @ Thu, 16 Apr 2020 01:23:40: 10000000 INFO @ Thu, 16 Apr 2020 01:23:41: 15000000 INFO @ Thu, 16 Apr 2020 01:23:48: 17000000 INFO @ Thu, 16 Apr 2020 01:23:51: 16000000 INFO @ Thu, 16 Apr 2020 01:23:52: 11000000 INFO @ Thu, 16 Apr 2020 01:24:00: 18000000 INFO @ Thu, 16 Apr 2020 01:24:00: 17000000 INFO @ Thu, 16 Apr 2020 01:24:04: 12000000 INFO @ Thu, 16 Apr 2020 01:24:10: 18000000 INFO @ Thu, 16 Apr 2020 01:24:11: 19000000 INFO @ Thu, 16 Apr 2020 01:24:15: 13000000 INFO @ Thu, 16 Apr 2020 01:24:20: 19000000 INFO @ Thu, 16 Apr 2020 01:24:22: 20000000 INFO @ Thu, 16 Apr 2020 01:24:27: 14000000 INFO @ Thu, 16 Apr 2020 01:24:30: 20000000 INFO @ Thu, 16 Apr 2020 01:24:34: 21000000 INFO @ Thu, 16 Apr 2020 01:24:37: 15000000 INFO @ Thu, 16 Apr 2020 01:24:40: 21000000 INFO @ Thu, 16 Apr 2020 01:24:45: 22000000 INFO @ Thu, 16 Apr 2020 01:24:48: 16000000 INFO @ Thu, 16 Apr 2020 01:24:49: 22000000 INFO @ Thu, 16 Apr 2020 01:24:56: 23000000 INFO @ Thu, 16 Apr 2020 01:24:59: 23000000 INFO @ Thu, 16 Apr 2020 01:24:59: 17000000 INFO @ Thu, 16 Apr 2020 01:25:07: 24000000 INFO @ Thu, 16 Apr 2020 01:25:08: 24000000 INFO @ Thu, 16 Apr 2020 01:25:10: 18000000 INFO @ Thu, 16 Apr 2020 01:25:19: 25000000 INFO @ Thu, 16 Apr 2020 01:25:19: 25000000 INFO @ Thu, 16 Apr 2020 01:25:22: 19000000 INFO @ Thu, 16 Apr 2020 01:25:28: 26000000 INFO @ Thu, 16 Apr 2020 01:25:30: 26000000 INFO @ Thu, 16 Apr 2020 01:25:33: 20000000 INFO @ Thu, 16 Apr 2020 01:25:38: 27000000 INFO @ Thu, 16 Apr 2020 01:25:42: 27000000 INFO @ Thu, 16 Apr 2020 01:25:44: 21000000 INFO @ Thu, 16 Apr 2020 01:25:48: 28000000 INFO @ Thu, 16 Apr 2020 01:25:53: 28000000 INFO @ Thu, 16 Apr 2020 01:25:55: 22000000 INFO @ Thu, 16 Apr 2020 01:25:57: 29000000 INFO @ Thu, 16 Apr 2020 01:26:04: 29000000 INFO @ Thu, 16 Apr 2020 01:26:05: 23000000 INFO @ Thu, 16 Apr 2020 01:26:06: 30000000 INFO @ Thu, 16 Apr 2020 01:26:15: 30000000 INFO @ Thu, 16 Apr 2020 01:26:16: 31000000 INFO @ Thu, 16 Apr 2020 01:26:17: 24000000 INFO @ Thu, 16 Apr 2020 01:26:25: 32000000 INFO @ Thu, 16 Apr 2020 01:26:26: 31000000 INFO @ Thu, 16 Apr 2020 01:26:28: 25000000 INFO @ Thu, 16 Apr 2020 01:26:35: 33000000 INFO @ Thu, 16 Apr 2020 01:26:38: 32000000 INFO @ Thu, 16 Apr 2020 01:26:39: 26000000 INFO @ Thu, 16 Apr 2020 01:26:45: 34000000 INFO @ Thu, 16 Apr 2020 01:26:49: 33000000 INFO @ Thu, 16 Apr 2020 01:26:51: 27000000 INFO @ Thu, 16 Apr 2020 01:26:54: 35000000 INFO @ Thu, 16 Apr 2020 01:27:01: 34000000 INFO @ Thu, 16 Apr 2020 01:27:02: 28000000 INFO @ Thu, 16 Apr 2020 01:27:04: 36000000 INFO @ Thu, 16 Apr 2020 01:27:12: 35000000 INFO @ Thu, 16 Apr 2020 01:27:13: 29000000 INFO @ Thu, 16 Apr 2020 01:27:14: 37000000 INFO @ Thu, 16 Apr 2020 01:27:23: 36000000 INFO @ Thu, 16 Apr 2020 01:27:24: 38000000 INFO @ Thu, 16 Apr 2020 01:27:24: 30000000 INFO @ Thu, 16 Apr 2020 01:27:33: 39000000 INFO @ Thu, 16 Apr 2020 01:27:34: 37000000 INFO @ Thu, 16 Apr 2020 01:27:35: 31000000 INFO @ Thu, 16 Apr 2020 01:27:43: 40000000 INFO @ Thu, 16 Apr 2020 01:27:45: 38000000 INFO @ Thu, 16 Apr 2020 01:27:46: 32000000 INFO @ Thu, 16 Apr 2020 01:27:52: 41000000 INFO @ Thu, 16 Apr 2020 01:27:56: 39000000 INFO @ Thu, 16 Apr 2020 01:27:58: 33000000 INFO @ Thu, 16 Apr 2020 01:28:01: 42000000 INFO @ Thu, 16 Apr 2020 01:28:07: 40000000 INFO @ Thu, 16 Apr 2020 01:28:10: 34000000 INFO @ Thu, 16 Apr 2020 01:28:11: 43000000 INFO @ Thu, 16 Apr 2020 01:28:18: 41000000 INFO @ Thu, 16 Apr 2020 01:28:20: 44000000 INFO @ Thu, 16 Apr 2020 01:28:21: 35000000 INFO @ Thu, 16 Apr 2020 01:28:28: 42000000 INFO @ Thu, 16 Apr 2020 01:28:29: 45000000 INFO @ Thu, 16 Apr 2020 01:28:32: 36000000 INFO @ Thu, 16 Apr 2020 01:28:39: 46000000 INFO @ Thu, 16 Apr 2020 01:28:39: 43000000 INFO @ Thu, 16 Apr 2020 01:28:43: 37000000 INFO @ Thu, 16 Apr 2020 01:28:49: 47000000 INFO @ Thu, 16 Apr 2020 01:28:49: 44000000 INFO @ Thu, 16 Apr 2020 01:28:55: 38000000 INFO @ Thu, 16 Apr 2020 01:28:58: 48000000 INFO @ Thu, 16 Apr 2020 01:29:00: 45000000 INFO @ Thu, 16 Apr 2020 01:29:07: 39000000 INFO @ Thu, 16 Apr 2020 01:29:07: 49000000 INFO @ Thu, 16 Apr 2020 01:29:11: 46000000 INFO @ Thu, 16 Apr 2020 01:29:17: 50000000 INFO @ Thu, 16 Apr 2020 01:29:18: 40000000 INFO @ Thu, 16 Apr 2020 01:29:23: 47000000 INFO @ Thu, 16 Apr 2020 01:29:27: 51000000 INFO @ Thu, 16 Apr 2020 01:29:29: 41000000 INFO @ Thu, 16 Apr 2020 01:29:33: 48000000 INFO @ Thu, 16 Apr 2020 01:29:38: 52000000 INFO @ Thu, 16 Apr 2020 01:29:40: 42000000 INFO @ Thu, 16 Apr 2020 01:29:44: 49000000 INFO @ Thu, 16 Apr 2020 01:29:48: 53000000 INFO @ Thu, 16 Apr 2020 01:29:51: 43000000 INFO @ Thu, 16 Apr 2020 01:29:54: 50000000 INFO @ Thu, 16 Apr 2020 01:29:57: 54000000 INFO @ Thu, 16 Apr 2020 01:30:01: 44000000 INFO @ Thu, 16 Apr 2020 01:30:05: 51000000 INFO @ Thu, 16 Apr 2020 01:30:07: 55000000 INFO @ Thu, 16 Apr 2020 01:30:12: 45000000 INFO @ Thu, 16 Apr 2020 01:30:15: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 01:30:15: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 01:30:15: #1 total tags in treatment: 16357293 INFO @ Thu, 16 Apr 2020 01:30:15: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 01:30:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 01:30:15: #1 tags after filtering in treatment: 12481761 INFO @ Thu, 16 Apr 2020 01:30:15: #1 Redundant rate of treatment: 0.24 INFO @ Thu, 16 Apr 2020 01:30:15: #1 finished! INFO @ Thu, 16 Apr 2020 01:30:15: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 01:30:15: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 01:30:15: 52000000 INFO @ Thu, 16 Apr 2020 01:30:16: #2 number of paired peaks: 332 WARNING @ Thu, 16 Apr 2020 01:30:16: Fewer paired peaks (332) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 332 pairs to build model! INFO @ Thu, 16 Apr 2020 01:30:16: start model_add_line... INFO @ Thu, 16 Apr 2020 01:30:16: start X-correlation... INFO @ Thu, 16 Apr 2020 01:30:16: end of X-cor INFO @ Thu, 16 Apr 2020 01:30:16: #2 finished! INFO @ Thu, 16 Apr 2020 01:30:16: #2 predicted fragment length is 201 bps INFO @ Thu, 16 Apr 2020 01:30:16: #2 alternative fragment length(s) may be 201 bps INFO @ Thu, 16 Apr 2020 01:30:16: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619573/SRX6619573.10_model.r WARNING @ Thu, 16 Apr 2020 01:30:16: #2 Since the d (201) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 01:30:16: #2 You may need to consider one of the other alternative d(s): 201 WARNING @ Thu, 16 Apr 2020 01:30:16: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 01:30:16: #3 Call peaks... INFO @ Thu, 16 Apr 2020 01:30:16: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 01:30:23: 46000000 INFO @ Thu, 16 Apr 2020 01:30:26: 53000000 INFO @ Thu, 16 Apr 2020 01:30:34: 47000000 INFO @ Thu, 16 Apr 2020 01:30:36: 54000000 INFO @ Thu, 16 Apr 2020 01:30:43: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 01:30:45: 48000000 INFO @ Thu, 16 Apr 2020 01:30:48: 55000000 INFO @ Thu, 16 Apr 2020 01:30:56: 49000000 INFO @ Thu, 16 Apr 2020 01:30:57: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 01:30:57: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 01:30:57: #1 total tags in treatment: 16357293 INFO @ Thu, 16 Apr 2020 01:30:57: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 01:30:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 01:30:57: #1 tags after filtering in treatment: 12481761 INFO @ Thu, 16 Apr 2020 01:30:57: #1 Redundant rate of treatment: 0.24 INFO @ Thu, 16 Apr 2020 01:30:57: #1 finished! INFO @ Thu, 16 Apr 2020 01:30:57: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 01:30:57: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 01:30:58: #2 number of paired peaks: 332 WARNING @ Thu, 16 Apr 2020 01:30:58: Fewer paired peaks (332) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 332 pairs to build model! INFO @ Thu, 16 Apr 2020 01:30:58: start model_add_line... INFO @ Thu, 16 Apr 2020 01:30:58: start X-correlation... INFO @ Thu, 16 Apr 2020 01:30:58: end of X-cor INFO @ Thu, 16 Apr 2020 01:30:58: #2 finished! INFO @ Thu, 16 Apr 2020 01:30:58: #2 predicted fragment length is 201 bps INFO @ Thu, 16 Apr 2020 01:30:58: #2 alternative fragment length(s) may be 201 bps INFO @ Thu, 16 Apr 2020 01:30:58: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619573/SRX6619573.05_model.r WARNING @ Thu, 16 Apr 2020 01:30:58: #2 Since the d (201) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 01:30:58: #2 You may need to consider one of the other alternative d(s): 201 WARNING @ Thu, 16 Apr 2020 01:30:58: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 01:30:58: #3 Call peaks... INFO @ Thu, 16 Apr 2020 01:30:58: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 01:30:58: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619573/SRX6619573.10_peaks.xls INFO @ Thu, 16 Apr 2020 01:30:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619573/SRX6619573.10_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 01:30:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619573/SRX6619573.10_summits.bed INFO @ Thu, 16 Apr 2020 01:30:58: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (412 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 01:31:05: 50000000 INFO @ Thu, 16 Apr 2020 01:31:14: 51000000 INFO @ Thu, 16 Apr 2020 01:31:24: 52000000 INFO @ Thu, 16 Apr 2020 01:31:26: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 01:31:33: 53000000 INFO @ Thu, 16 Apr 2020 01:31:42: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619573/SRX6619573.05_peaks.xls INFO @ Thu, 16 Apr 2020 01:31:42: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619573/SRX6619573.05_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 01:31:42: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619573/SRX6619573.05_summits.bed INFO @ Thu, 16 Apr 2020 01:31:42: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (850 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 01:31:42: 54000000 INFO @ Thu, 16 Apr 2020 01:31:52: 55000000 INFO @ Thu, 16 Apr 2020 01:32:00: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 01:32:00: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 01:32:00: #1 total tags in treatment: 16357293 INFO @ Thu, 16 Apr 2020 01:32:00: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 01:32:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 01:32:00: #1 tags after filtering in treatment: 12481761 INFO @ Thu, 16 Apr 2020 01:32:00: #1 Redundant rate of treatment: 0.24 INFO @ Thu, 16 Apr 2020 01:32:00: #1 finished! INFO @ Thu, 16 Apr 2020 01:32:00: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 01:32:00: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 01:32:01: #2 number of paired peaks: 332 WARNING @ Thu, 16 Apr 2020 01:32:01: Fewer paired peaks (332) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 332 pairs to build model! INFO @ Thu, 16 Apr 2020 01:32:01: start model_add_line... INFO @ Thu, 16 Apr 2020 01:32:01: start X-correlation... INFO @ Thu, 16 Apr 2020 01:32:01: end of X-cor INFO @ Thu, 16 Apr 2020 01:32:01: #2 finished! INFO @ Thu, 16 Apr 2020 01:32:01: #2 predicted fragment length is 201 bps INFO @ Thu, 16 Apr 2020 01:32:01: #2 alternative fragment length(s) may be 201 bps INFO @ Thu, 16 Apr 2020 01:32:01: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619573/SRX6619573.20_model.r WARNING @ Thu, 16 Apr 2020 01:32:01: #2 Since the d (201) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 01:32:01: #2 You may need to consider one of the other alternative d(s): 201 WARNING @ Thu, 16 Apr 2020 01:32:01: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 01:32:01: #3 Call peaks... INFO @ Thu, 16 Apr 2020 01:32:01: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 01:32:28: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 01:32:43: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619573/SRX6619573.20_peaks.xls INFO @ Thu, 16 Apr 2020 01:32:43: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619573/SRX6619573.20_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 01:32:43: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619573/SRX6619573.20_summits.bed INFO @ Thu, 16 Apr 2020 01:32:43: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (220 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。