Job ID = 5720202 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-04-15T12:25:31 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:38:33 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:39:34 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:48:39 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:54:35 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:56:28 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:12:03 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:16:03 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 33,979,047 reads read : 67,958,094 reads written : 67,958,094 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:12:54 33979047 reads; of these: 33979047 (100.00%) were paired; of these: 16235776 (47.78%) aligned concordantly 0 times 13149784 (38.70%) aligned concordantly exactly 1 time 4593487 (13.52%) aligned concordantly >1 times ---- 16235776 pairs aligned concordantly 0 times; of these: 7145479 (44.01%) aligned discordantly 1 time ---- 9090297 pairs aligned 0 times concordantly or discordantly; of these: 18180594 mates make up the pairs; of these: 14839753 (81.62%) aligned 0 times 1171094 (6.44%) aligned exactly 1 time 2169747 (11.93%) aligned >1 times 78.16% overall alignment rate Time searching: 01:12:54 Overall time: 01:12:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 3805838 / 24534173 = 0.1551 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:09:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619571/SRX6619571.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619571/SRX6619571.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619571/SRX6619571.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619571/SRX6619571.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:09:20: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:09:20: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:09:27: 1000000 INFO @ Thu, 16 Apr 2020 00:09:33: 2000000 INFO @ Thu, 16 Apr 2020 00:09:39: 3000000 INFO @ Thu, 16 Apr 2020 00:09:45: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:09:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619571/SRX6619571.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619571/SRX6619571.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619571/SRX6619571.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619571/SRX6619571.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:09:50: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:09:50: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:09:52: 5000000 INFO @ Thu, 16 Apr 2020 00:09:57: 1000000 INFO @ Thu, 16 Apr 2020 00:09:59: 6000000 INFO @ Thu, 16 Apr 2020 00:10:03: 2000000 INFO @ Thu, 16 Apr 2020 00:10:06: 7000000 INFO @ Thu, 16 Apr 2020 00:10:09: 3000000 INFO @ Thu, 16 Apr 2020 00:10:12: 8000000 INFO @ Thu, 16 Apr 2020 00:10:16: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:10:19: 9000000 INFO @ Thu, 16 Apr 2020 00:10:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619571/SRX6619571.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619571/SRX6619571.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619571/SRX6619571.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619571/SRX6619571.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:10:20: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:10:20: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:10:22: 5000000 INFO @ Thu, 16 Apr 2020 00:10:27: 10000000 INFO @ Thu, 16 Apr 2020 00:10:28: 1000000 INFO @ Thu, 16 Apr 2020 00:10:29: 6000000 INFO @ Thu, 16 Apr 2020 00:10:35: 11000000 INFO @ Thu, 16 Apr 2020 00:10:36: 7000000 INFO @ Thu, 16 Apr 2020 00:10:36: 2000000 INFO @ Thu, 16 Apr 2020 00:10:42: 8000000 INFO @ Thu, 16 Apr 2020 00:10:43: 12000000 INFO @ Thu, 16 Apr 2020 00:10:44: 3000000 INFO @ Thu, 16 Apr 2020 00:10:49: 9000000 INFO @ Thu, 16 Apr 2020 00:10:51: 13000000 INFO @ Thu, 16 Apr 2020 00:10:52: 4000000 INFO @ Thu, 16 Apr 2020 00:10:55: 10000000 INFO @ Thu, 16 Apr 2020 00:10:59: 14000000 INFO @ Thu, 16 Apr 2020 00:11:00: 5000000 INFO @ Thu, 16 Apr 2020 00:11:02: 11000000 INFO @ Thu, 16 Apr 2020 00:11:08: 15000000 INFO @ Thu, 16 Apr 2020 00:11:08: 6000000 INFO @ Thu, 16 Apr 2020 00:11:09: 12000000 INFO @ Thu, 16 Apr 2020 00:11:15: 13000000 INFO @ Thu, 16 Apr 2020 00:11:16: 16000000 INFO @ Thu, 16 Apr 2020 00:11:16: 7000000 INFO @ Thu, 16 Apr 2020 00:11:22: 14000000 INFO @ Thu, 16 Apr 2020 00:11:24: 17000000 INFO @ Thu, 16 Apr 2020 00:11:24: 8000000 INFO @ Thu, 16 Apr 2020 00:11:28: 15000000 INFO @ Thu, 16 Apr 2020 00:11:32: 18000000 INFO @ Thu, 16 Apr 2020 00:11:32: 9000000 INFO @ Thu, 16 Apr 2020 00:11:35: 16000000 INFO @ Thu, 16 Apr 2020 00:11:40: 19000000 INFO @ Thu, 16 Apr 2020 00:11:40: 10000000 INFO @ Thu, 16 Apr 2020 00:11:41: 17000000 INFO @ Thu, 16 Apr 2020 00:11:47: 20000000 INFO @ Thu, 16 Apr 2020 00:11:48: 18000000 INFO @ Thu, 16 Apr 2020 00:11:48: 11000000 INFO @ Thu, 16 Apr 2020 00:11:54: 19000000 INFO @ Thu, 16 Apr 2020 00:11:55: 21000000 INFO @ Thu, 16 Apr 2020 00:11:56: 12000000 INFO @ Thu, 16 Apr 2020 00:12:01: 20000000 INFO @ Thu, 16 Apr 2020 00:12:02: 22000000 INFO @ Thu, 16 Apr 2020 00:12:04: 13000000 INFO @ Thu, 16 Apr 2020 00:12:07: 21000000 INFO @ Thu, 16 Apr 2020 00:12:10: 23000000 INFO @ Thu, 16 Apr 2020 00:12:12: 14000000 INFO @ Thu, 16 Apr 2020 00:12:14: 22000000 INFO @ Thu, 16 Apr 2020 00:12:17: 24000000 INFO @ Thu, 16 Apr 2020 00:12:20: 15000000 INFO @ Thu, 16 Apr 2020 00:12:20: 23000000 INFO @ Thu, 16 Apr 2020 00:12:25: 25000000 INFO @ Thu, 16 Apr 2020 00:12:27: 24000000 INFO @ Thu, 16 Apr 2020 00:12:28: 16000000 INFO @ Thu, 16 Apr 2020 00:12:32: 26000000 INFO @ Thu, 16 Apr 2020 00:12:33: 25000000 INFO @ Thu, 16 Apr 2020 00:12:36: 17000000 INFO @ Thu, 16 Apr 2020 00:12:40: 26000000 INFO @ Thu, 16 Apr 2020 00:12:40: 27000000 INFO @ Thu, 16 Apr 2020 00:12:44: 18000000 INFO @ Thu, 16 Apr 2020 00:12:46: 27000000 INFO @ Thu, 16 Apr 2020 00:12:48: 28000000 INFO @ Thu, 16 Apr 2020 00:12:52: 19000000 INFO @ Thu, 16 Apr 2020 00:12:53: 28000000 INFO @ Thu, 16 Apr 2020 00:12:55: 29000000 INFO @ Thu, 16 Apr 2020 00:12:59: 29000000 INFO @ Thu, 16 Apr 2020 00:13:00: 20000000 INFO @ Thu, 16 Apr 2020 00:13:02: 30000000 INFO @ Thu, 16 Apr 2020 00:13:06: 30000000 INFO @ Thu, 16 Apr 2020 00:13:08: 21000000 INFO @ Thu, 16 Apr 2020 00:13:10: 31000000 INFO @ Thu, 16 Apr 2020 00:13:12: 31000000 INFO @ Thu, 16 Apr 2020 00:13:16: 22000000 INFO @ Thu, 16 Apr 2020 00:13:17: 32000000 INFO @ Thu, 16 Apr 2020 00:13:18: 32000000 INFO @ Thu, 16 Apr 2020 00:13:23: 23000000 INFO @ Thu, 16 Apr 2020 00:13:25: 33000000 INFO @ Thu, 16 Apr 2020 00:13:25: 33000000 INFO @ Thu, 16 Apr 2020 00:13:31: 24000000 INFO @ Thu, 16 Apr 2020 00:13:32: 34000000 INFO @ Thu, 16 Apr 2020 00:13:32: 34000000 INFO @ Thu, 16 Apr 2020 00:13:39: 25000000 INFO @ Thu, 16 Apr 2020 00:13:39: 35000000 INFO @ Thu, 16 Apr 2020 00:13:40: 35000000 INFO @ Thu, 16 Apr 2020 00:13:47: 36000000 INFO @ Thu, 16 Apr 2020 00:13:47: 26000000 INFO @ Thu, 16 Apr 2020 00:13:47: 36000000 INFO @ Thu, 16 Apr 2020 00:13:54: 37000000 INFO @ Thu, 16 Apr 2020 00:13:55: 37000000 INFO @ Thu, 16 Apr 2020 00:13:55: 27000000 INFO @ Thu, 16 Apr 2020 00:14:02: 38000000 INFO @ Thu, 16 Apr 2020 00:14:02: 38000000 INFO @ Thu, 16 Apr 2020 00:14:03: 28000000 INFO @ Thu, 16 Apr 2020 00:14:09: 39000000 INFO @ Thu, 16 Apr 2020 00:14:10: 39000000 INFO @ Thu, 16 Apr 2020 00:14:11: 29000000 INFO @ Thu, 16 Apr 2020 00:14:17: 40000000 INFO @ Thu, 16 Apr 2020 00:14:18: 40000000 INFO @ Thu, 16 Apr 2020 00:14:18: 30000000 INFO @ Thu, 16 Apr 2020 00:14:25: 41000000 INFO @ Thu, 16 Apr 2020 00:14:25: 41000000 INFO @ Thu, 16 Apr 2020 00:14:26: 31000000 INFO @ Thu, 16 Apr 2020 00:14:32: 42000000 INFO @ Thu, 16 Apr 2020 00:14:33: 42000000 INFO @ Thu, 16 Apr 2020 00:14:34: 32000000 INFO @ Thu, 16 Apr 2020 00:14:40: 43000000 INFO @ Thu, 16 Apr 2020 00:14:40: 43000000 INFO @ Thu, 16 Apr 2020 00:14:41: 33000000 INFO @ Thu, 16 Apr 2020 00:14:48: 44000000 INFO @ Thu, 16 Apr 2020 00:14:48: 44000000 INFO @ Thu, 16 Apr 2020 00:14:49: 34000000 INFO @ Thu, 16 Apr 2020 00:14:55: 45000000 INFO @ Thu, 16 Apr 2020 00:14:56: 45000000 INFO @ Thu, 16 Apr 2020 00:14:56: 35000000 INFO @ Thu, 16 Apr 2020 00:14:59: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:14:59: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:14:59: #1 total tags in treatment: 14700013 INFO @ Thu, 16 Apr 2020 00:14:59: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:14:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:14:59: #1 tags after filtering in treatment: 11339184 INFO @ Thu, 16 Apr 2020 00:14:59: #1 Redundant rate of treatment: 0.23 INFO @ Thu, 16 Apr 2020 00:14:59: #1 finished! INFO @ Thu, 16 Apr 2020 00:14:59: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:14:59: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:14:59: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:14:59: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:14:59: #1 total tags in treatment: 14700013 INFO @ Thu, 16 Apr 2020 00:14:59: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:14:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:15:00: #1 tags after filtering in treatment: 11339184 INFO @ Thu, 16 Apr 2020 00:15:00: #1 Redundant rate of treatment: 0.23 INFO @ Thu, 16 Apr 2020 00:15:00: #1 finished! INFO @ Thu, 16 Apr 2020 00:15:00: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:15:00: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:15:00: #2 number of paired peaks: 450 WARNING @ Thu, 16 Apr 2020 00:15:00: Fewer paired peaks (450) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 450 pairs to build model! INFO @ Thu, 16 Apr 2020 00:15:00: start model_add_line... INFO @ Thu, 16 Apr 2020 00:15:00: start X-correlation... INFO @ Thu, 16 Apr 2020 00:15:00: end of X-cor INFO @ Thu, 16 Apr 2020 00:15:00: #2 finished! INFO @ Thu, 16 Apr 2020 00:15:00: #2 predicted fragment length is 212 bps INFO @ Thu, 16 Apr 2020 00:15:00: #2 alternative fragment length(s) may be 212,225 bps INFO @ Thu, 16 Apr 2020 00:15:00: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619571/SRX6619571.05_model.r WARNING @ Thu, 16 Apr 2020 00:15:00: #2 Since the d (212) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:15:00: #2 You may need to consider one of the other alternative d(s): 212,225 WARNING @ Thu, 16 Apr 2020 00:15:00: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:15:00: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:15:00: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:15:00: #2 number of paired peaks: 450 WARNING @ Thu, 16 Apr 2020 00:15:00: Fewer paired peaks (450) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 450 pairs to build model! INFO @ Thu, 16 Apr 2020 00:15:00: start model_add_line... INFO @ Thu, 16 Apr 2020 00:15:00: start X-correlation... INFO @ Thu, 16 Apr 2020 00:15:00: end of X-cor INFO @ Thu, 16 Apr 2020 00:15:00: #2 finished! INFO @ Thu, 16 Apr 2020 00:15:00: #2 predicted fragment length is 212 bps INFO @ Thu, 16 Apr 2020 00:15:00: #2 alternative fragment length(s) may be 212,225 bps INFO @ Thu, 16 Apr 2020 00:15:01: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619571/SRX6619571.10_model.r WARNING @ Thu, 16 Apr 2020 00:15:01: #2 Since the d (212) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:15:01: #2 You may need to consider one of the other alternative d(s): 212,225 WARNING @ Thu, 16 Apr 2020 00:15:01: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:15:01: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:15:01: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:15:04: 36000000 INFO @ Thu, 16 Apr 2020 00:15:12: 37000000 INFO @ Thu, 16 Apr 2020 00:15:19: 38000000 INFO @ Thu, 16 Apr 2020 00:15:25: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:15:26: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:15:27: 39000000 INFO @ Thu, 16 Apr 2020 00:15:35: 40000000 INFO @ Thu, 16 Apr 2020 00:15:36: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619571/SRX6619571.10_peaks.xls INFO @ Thu, 16 Apr 2020 00:15:36: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619571/SRX6619571.10_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:15:36: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619571/SRX6619571.10_summits.bed INFO @ Thu, 16 Apr 2020 00:15:36: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (540 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:15:38: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619571/SRX6619571.05_peaks.xls INFO @ Thu, 16 Apr 2020 00:15:38: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619571/SRX6619571.05_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:15:38: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619571/SRX6619571.05_summits.bed INFO @ Thu, 16 Apr 2020 00:15:38: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (1095 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:15:42: 41000000 INFO @ Thu, 16 Apr 2020 00:15:50: 42000000 INFO @ Thu, 16 Apr 2020 00:15:58: 43000000 INFO @ Thu, 16 Apr 2020 00:16:05: 44000000 INFO @ Thu, 16 Apr 2020 00:16:13: 45000000 INFO @ Thu, 16 Apr 2020 00:16:17: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:16:17: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:16:17: #1 total tags in treatment: 14700013 INFO @ Thu, 16 Apr 2020 00:16:17: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:16:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:16:17: #1 tags after filtering in treatment: 11339184 INFO @ Thu, 16 Apr 2020 00:16:17: #1 Redundant rate of treatment: 0.23 INFO @ Thu, 16 Apr 2020 00:16:17: #1 finished! INFO @ Thu, 16 Apr 2020 00:16:17: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:16:17: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:16:18: #2 number of paired peaks: 450 WARNING @ Thu, 16 Apr 2020 00:16:18: Fewer paired peaks (450) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 450 pairs to build model! INFO @ Thu, 16 Apr 2020 00:16:18: start model_add_line... INFO @ Thu, 16 Apr 2020 00:16:18: start X-correlation... INFO @ Thu, 16 Apr 2020 00:16:18: end of X-cor INFO @ Thu, 16 Apr 2020 00:16:18: #2 finished! INFO @ Thu, 16 Apr 2020 00:16:18: #2 predicted fragment length is 212 bps INFO @ Thu, 16 Apr 2020 00:16:18: #2 alternative fragment length(s) may be 212,225 bps INFO @ Thu, 16 Apr 2020 00:16:18: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619571/SRX6619571.20_model.r WARNING @ Thu, 16 Apr 2020 00:16:18: #2 Since the d (212) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:16:18: #2 You may need to consider one of the other alternative d(s): 212,225 WARNING @ Thu, 16 Apr 2020 00:16:18: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:16:18: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:16:18: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:16:43: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:16:54: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619571/SRX6619571.20_peaks.xls INFO @ Thu, 16 Apr 2020 00:16:54: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619571/SRX6619571.20_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:16:54: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619571/SRX6619571.20_summits.bed INFO @ Thu, 16 Apr 2020 00:16:54: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (305 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。