Job ID = 5720182 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-04-15T12:48:47 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:52:13 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:53:45 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:12:48 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:17:49 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 33,886,652 reads read : 67,773,304 reads written : 67,773,304 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:19:45 33886652 reads; of these: 33886652 (100.00%) were paired; of these: 18906388 (55.79%) aligned concordantly 0 times 12341640 (36.42%) aligned concordantly exactly 1 time 2638624 (7.79%) aligned concordantly >1 times ---- 18906388 pairs aligned concordantly 0 times; of these: 9162250 (48.46%) aligned discordantly 1 time ---- 9744138 pairs aligned 0 times concordantly or discordantly; of these: 19488276 mates make up the pairs; of these: 16265076 (83.46%) aligned 0 times 1339859 (6.88%) aligned exactly 1 time 1883341 (9.66%) aligned >1 times 76.00% overall alignment rate Time searching: 01:19:45 Overall time: 01:19:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 2865472 / 23571129 = 0.1216 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:13:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619553/SRX6619553.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619553/SRX6619553.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619553/SRX6619553.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619553/SRX6619553.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:13:53: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:13:53: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:14:00: 1000000 INFO @ Thu, 16 Apr 2020 00:14:07: 2000000 INFO @ Thu, 16 Apr 2020 00:14:15: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:14:22: 4000000 INFO @ Thu, 16 Apr 2020 00:14:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619553/SRX6619553.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619553/SRX6619553.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619553/SRX6619553.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619553/SRX6619553.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:14:22: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:14:22: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:14:30: 5000000 INFO @ Thu, 16 Apr 2020 00:14:30: 1000000 INFO @ Thu, 16 Apr 2020 00:14:38: 6000000 INFO @ Thu, 16 Apr 2020 00:14:39: 2000000 INFO @ Thu, 16 Apr 2020 00:14:46: 7000000 INFO @ Thu, 16 Apr 2020 00:14:47: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:14:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619553/SRX6619553.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619553/SRX6619553.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619553/SRX6619553.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619553/SRX6619553.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:14:52: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:14:52: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:14:54: 8000000 INFO @ Thu, 16 Apr 2020 00:14:56: 4000000 INFO @ Thu, 16 Apr 2020 00:15:01: 1000000 INFO @ Thu, 16 Apr 2020 00:15:03: 9000000 INFO @ Thu, 16 Apr 2020 00:15:05: 5000000 INFO @ Thu, 16 Apr 2020 00:15:11: 2000000 INFO @ Thu, 16 Apr 2020 00:15:13: 10000000 INFO @ Thu, 16 Apr 2020 00:15:15: 6000000 INFO @ Thu, 16 Apr 2020 00:15:21: 3000000 INFO @ Thu, 16 Apr 2020 00:15:22: 11000000 INFO @ Thu, 16 Apr 2020 00:15:24: 7000000 INFO @ Thu, 16 Apr 2020 00:15:30: 4000000 INFO @ Thu, 16 Apr 2020 00:15:31: 12000000 INFO @ Thu, 16 Apr 2020 00:15:34: 8000000 INFO @ Thu, 16 Apr 2020 00:15:39: 5000000 INFO @ Thu, 16 Apr 2020 00:15:39: 13000000 INFO @ Thu, 16 Apr 2020 00:15:43: 9000000 INFO @ Thu, 16 Apr 2020 00:15:48: 14000000 INFO @ Thu, 16 Apr 2020 00:15:48: 6000000 INFO @ Thu, 16 Apr 2020 00:15:51: 10000000 INFO @ Thu, 16 Apr 2020 00:15:57: 15000000 INFO @ Thu, 16 Apr 2020 00:15:58: 7000000 INFO @ Thu, 16 Apr 2020 00:16:01: 11000000 INFO @ Thu, 16 Apr 2020 00:16:07: 16000000 INFO @ Thu, 16 Apr 2020 00:16:07: 8000000 INFO @ Thu, 16 Apr 2020 00:16:10: 12000000 INFO @ Thu, 16 Apr 2020 00:16:16: 17000000 INFO @ Thu, 16 Apr 2020 00:16:17: 9000000 INFO @ Thu, 16 Apr 2020 00:16:19: 13000000 INFO @ Thu, 16 Apr 2020 00:16:25: 18000000 INFO @ Thu, 16 Apr 2020 00:16:26: 10000000 INFO @ Thu, 16 Apr 2020 00:16:29: 14000000 INFO @ Thu, 16 Apr 2020 00:16:34: 19000000 INFO @ Thu, 16 Apr 2020 00:16:35: 11000000 INFO @ Thu, 16 Apr 2020 00:16:38: 15000000 INFO @ Thu, 16 Apr 2020 00:16:43: 20000000 INFO @ Thu, 16 Apr 2020 00:16:45: 12000000 INFO @ Thu, 16 Apr 2020 00:16:48: 16000000 INFO @ Thu, 16 Apr 2020 00:16:52: 21000000 INFO @ Thu, 16 Apr 2020 00:16:54: 13000000 INFO @ Thu, 16 Apr 2020 00:16:57: 17000000 INFO @ Thu, 16 Apr 2020 00:17:02: 22000000 INFO @ Thu, 16 Apr 2020 00:17:03: 14000000 INFO @ Thu, 16 Apr 2020 00:17:06: 18000000 INFO @ Thu, 16 Apr 2020 00:17:10: 23000000 INFO @ Thu, 16 Apr 2020 00:17:12: 15000000 INFO @ Thu, 16 Apr 2020 00:17:15: 19000000 INFO @ Thu, 16 Apr 2020 00:17:19: 24000000 INFO @ Thu, 16 Apr 2020 00:17:21: 16000000 INFO @ Thu, 16 Apr 2020 00:17:24: 20000000 INFO @ Thu, 16 Apr 2020 00:17:28: 25000000 INFO @ Thu, 16 Apr 2020 00:17:30: 17000000 INFO @ Thu, 16 Apr 2020 00:17:33: 21000000 INFO @ Thu, 16 Apr 2020 00:17:37: 26000000 INFO @ Thu, 16 Apr 2020 00:17:40: 18000000 INFO @ Thu, 16 Apr 2020 00:17:43: 22000000 INFO @ Thu, 16 Apr 2020 00:17:46: 27000000 INFO @ Thu, 16 Apr 2020 00:17:49: 19000000 INFO @ Thu, 16 Apr 2020 00:17:52: 23000000 INFO @ Thu, 16 Apr 2020 00:17:56: 28000000 INFO @ Thu, 16 Apr 2020 00:17:58: 20000000 INFO @ Thu, 16 Apr 2020 00:18:01: 24000000 INFO @ Thu, 16 Apr 2020 00:18:05: 29000000 INFO @ Thu, 16 Apr 2020 00:18:07: 21000000 INFO @ Thu, 16 Apr 2020 00:18:10: 25000000 INFO @ Thu, 16 Apr 2020 00:18:13: 30000000 INFO @ Thu, 16 Apr 2020 00:18:17: 22000000 INFO @ Thu, 16 Apr 2020 00:18:19: 26000000 INFO @ Thu, 16 Apr 2020 00:18:22: 31000000 INFO @ Thu, 16 Apr 2020 00:18:26: 23000000 INFO @ Thu, 16 Apr 2020 00:18:28: 27000000 INFO @ Thu, 16 Apr 2020 00:18:31: 32000000 INFO @ Thu, 16 Apr 2020 00:18:35: 24000000 INFO @ Thu, 16 Apr 2020 00:18:38: 28000000 INFO @ Thu, 16 Apr 2020 00:18:40: 33000000 INFO @ Thu, 16 Apr 2020 00:18:45: 25000000 INFO @ Thu, 16 Apr 2020 00:18:47: 29000000 INFO @ Thu, 16 Apr 2020 00:18:49: 34000000 INFO @ Thu, 16 Apr 2020 00:18:54: 26000000 INFO @ Thu, 16 Apr 2020 00:18:56: 30000000 INFO @ Thu, 16 Apr 2020 00:18:58: 35000000 INFO @ Thu, 16 Apr 2020 00:19:04: 27000000 INFO @ Thu, 16 Apr 2020 00:19:05: 31000000 INFO @ Thu, 16 Apr 2020 00:19:07: 36000000 INFO @ Thu, 16 Apr 2020 00:19:13: 28000000 INFO @ Thu, 16 Apr 2020 00:19:15: 32000000 INFO @ Thu, 16 Apr 2020 00:19:16: 37000000 INFO @ Thu, 16 Apr 2020 00:19:22: 29000000 INFO @ Thu, 16 Apr 2020 00:19:24: 33000000 INFO @ Thu, 16 Apr 2020 00:19:25: 38000000 INFO @ Thu, 16 Apr 2020 00:19:31: 30000000 INFO @ Thu, 16 Apr 2020 00:19:32: 34000000 INFO @ Thu, 16 Apr 2020 00:19:33: 39000000 INFO @ Thu, 16 Apr 2020 00:19:40: 31000000 INFO @ Thu, 16 Apr 2020 00:19:41: 35000000 INFO @ Thu, 16 Apr 2020 00:19:42: 40000000 INFO @ Thu, 16 Apr 2020 00:19:49: 32000000 INFO @ Thu, 16 Apr 2020 00:19:50: 36000000 INFO @ Thu, 16 Apr 2020 00:19:51: 41000000 INFO @ Thu, 16 Apr 2020 00:19:58: 33000000 INFO @ Thu, 16 Apr 2020 00:19:59: 37000000 INFO @ Thu, 16 Apr 2020 00:19:59: 42000000 INFO @ Thu, 16 Apr 2020 00:20:08: 34000000 INFO @ Thu, 16 Apr 2020 00:20:08: 38000000 INFO @ Thu, 16 Apr 2020 00:20:09: 43000000 INFO @ Thu, 16 Apr 2020 00:20:17: 35000000 INFO @ Thu, 16 Apr 2020 00:20:18: 39000000 INFO @ Thu, 16 Apr 2020 00:20:18: 44000000 INFO @ Thu, 16 Apr 2020 00:20:26: 36000000 INFO @ Thu, 16 Apr 2020 00:20:27: 40000000 INFO @ Thu, 16 Apr 2020 00:20:27: 45000000 INFO @ Thu, 16 Apr 2020 00:20:34: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:20:34: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:20:34: #1 total tags in treatment: 13010477 INFO @ Thu, 16 Apr 2020 00:20:34: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:20:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:20:34: #1 tags after filtering in treatment: 11131570 INFO @ Thu, 16 Apr 2020 00:20:34: #1 Redundant rate of treatment: 0.14 INFO @ Thu, 16 Apr 2020 00:20:34: #1 finished! INFO @ Thu, 16 Apr 2020 00:20:34: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:20:34: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:20:35: #2 number of paired peaks: 319 WARNING @ Thu, 16 Apr 2020 00:20:35: Fewer paired peaks (319) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 319 pairs to build model! INFO @ Thu, 16 Apr 2020 00:20:35: start model_add_line... INFO @ Thu, 16 Apr 2020 00:20:35: 37000000 INFO @ Thu, 16 Apr 2020 00:20:35: start X-correlation... INFO @ Thu, 16 Apr 2020 00:20:35: end of X-cor INFO @ Thu, 16 Apr 2020 00:20:35: #2 finished! INFO @ Thu, 16 Apr 2020 00:20:35: #2 predicted fragment length is 204 bps INFO @ Thu, 16 Apr 2020 00:20:35: #2 alternative fragment length(s) may be 204 bps INFO @ Thu, 16 Apr 2020 00:20:35: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619553/SRX6619553.05_model.r WARNING @ Thu, 16 Apr 2020 00:20:35: #2 Since the d (204) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:20:35: #2 You may need to consider one of the other alternative d(s): 204 WARNING @ Thu, 16 Apr 2020 00:20:35: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:20:35: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:20:35: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:20:36: 41000000 INFO @ Thu, 16 Apr 2020 00:20:43: 38000000 INFO @ Thu, 16 Apr 2020 00:20:43: 42000000 INFO @ Thu, 16 Apr 2020 00:20:51: 43000000 INFO @ Thu, 16 Apr 2020 00:20:51: 39000000 INFO @ Thu, 16 Apr 2020 00:20:57: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:21:00: 44000000 INFO @ Thu, 16 Apr 2020 00:21:01: 40000000 INFO @ Thu, 16 Apr 2020 00:21:08: 45000000 INFO @ Thu, 16 Apr 2020 00:21:08: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619553/SRX6619553.05_peaks.xls INFO @ Thu, 16 Apr 2020 00:21:09: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619553/SRX6619553.05_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:21:09: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619553/SRX6619553.05_summits.bed INFO @ Thu, 16 Apr 2020 00:21:09: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (521 records, 4 fields): 47 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:21:09: 41000000 INFO @ Thu, 16 Apr 2020 00:21:15: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:21:15: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:21:15: #1 total tags in treatment: 13010477 INFO @ Thu, 16 Apr 2020 00:21:15: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:21:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:21:15: #1 tags after filtering in treatment: 11131570 INFO @ Thu, 16 Apr 2020 00:21:15: #1 Redundant rate of treatment: 0.14 INFO @ Thu, 16 Apr 2020 00:21:15: #1 finished! INFO @ Thu, 16 Apr 2020 00:21:15: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:21:15: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:21:16: #2 number of paired peaks: 319 WARNING @ Thu, 16 Apr 2020 00:21:16: Fewer paired peaks (319) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 319 pairs to build model! INFO @ Thu, 16 Apr 2020 00:21:16: start model_add_line... INFO @ Thu, 16 Apr 2020 00:21:16: start X-correlation... INFO @ Thu, 16 Apr 2020 00:21:16: end of X-cor INFO @ Thu, 16 Apr 2020 00:21:16: #2 finished! INFO @ Thu, 16 Apr 2020 00:21:16: #2 predicted fragment length is 204 bps INFO @ Thu, 16 Apr 2020 00:21:16: #2 alternative fragment length(s) may be 204 bps INFO @ Thu, 16 Apr 2020 00:21:16: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619553/SRX6619553.10_model.r WARNING @ Thu, 16 Apr 2020 00:21:16: #2 Since the d (204) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:21:16: #2 You may need to consider one of the other alternative d(s): 204 WARNING @ Thu, 16 Apr 2020 00:21:16: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:21:16: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:21:16: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:21:17: 42000000 INFO @ Thu, 16 Apr 2020 00:21:24: 43000000 INFO @ Thu, 16 Apr 2020 00:21:32: 44000000 INFO @ Thu, 16 Apr 2020 00:21:38: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:21:39: 45000000 INFO @ Thu, 16 Apr 2020 00:21:45: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:21:45: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:21:45: #1 total tags in treatment: 13010477 INFO @ Thu, 16 Apr 2020 00:21:45: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:21:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:21:45: #1 tags after filtering in treatment: 11131570 INFO @ Thu, 16 Apr 2020 00:21:45: #1 Redundant rate of treatment: 0.14 INFO @ Thu, 16 Apr 2020 00:21:45: #1 finished! INFO @ Thu, 16 Apr 2020 00:21:45: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:21:45: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:21:46: #2 number of paired peaks: 319 WARNING @ Thu, 16 Apr 2020 00:21:46: Fewer paired peaks (319) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 319 pairs to build model! INFO @ Thu, 16 Apr 2020 00:21:46: start model_add_line... INFO @ Thu, 16 Apr 2020 00:21:46: start X-correlation... INFO @ Thu, 16 Apr 2020 00:21:46: end of X-cor INFO @ Thu, 16 Apr 2020 00:21:46: #2 finished! INFO @ Thu, 16 Apr 2020 00:21:46: #2 predicted fragment length is 204 bps INFO @ Thu, 16 Apr 2020 00:21:46: #2 alternative fragment length(s) may be 204 bps INFO @ Thu, 16 Apr 2020 00:21:46: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619553/SRX6619553.20_model.r WARNING @ Thu, 16 Apr 2020 00:21:46: #2 Since the d (204) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:21:46: #2 You may need to consider one of the other alternative d(s): 204 WARNING @ Thu, 16 Apr 2020 00:21:46: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:21:46: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:21:46: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:21:49: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619553/SRX6619553.10_peaks.xls INFO @ Thu, 16 Apr 2020 00:21:49: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619553/SRX6619553.10_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:21:49: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619553/SRX6619553.10_summits.bed INFO @ Thu, 16 Apr 2020 00:21:49: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (369 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:22:08: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:22:19: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619553/SRX6619553.20_peaks.xls INFO @ Thu, 16 Apr 2020 00:22:19: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619553/SRX6619553.20_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:22:19: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619553/SRX6619553.20_summits.bed INFO @ Thu, 16 Apr 2020 00:22:19: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (225 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。