Job ID = 5720178 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-04-15T12:23:17 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:28:14 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:30:00 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:07:14 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 29,499,160 reads read : 58,998,320 reads written : 58,998,320 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:14:28 29499160 reads; of these: 29499160 (100.00%) were paired; of these: 12655112 (42.90%) aligned concordantly 0 times 14260247 (48.34%) aligned concordantly exactly 1 time 2583801 (8.76%) aligned concordantly >1 times ---- 12655112 pairs aligned concordantly 0 times; of these: 7641411 (60.38%) aligned discordantly 1 time ---- 5013701 pairs aligned 0 times concordantly or discordantly; of these: 10027402 mates make up the pairs; of these: 7288955 (72.69%) aligned 0 times 1311732 (13.08%) aligned exactly 1 time 1426715 (14.23%) aligned >1 times 87.65% overall alignment rate Time searching: 01:14:28 Overall time: 01:14:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 2627399 / 23811884 = 0.1103 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 15 Apr 2020 23:59:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619549/SRX6619549.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619549/SRX6619549.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619549/SRX6619549.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619549/SRX6619549.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 15 Apr 2020 23:59:58: #1 read tag files... INFO @ Wed, 15 Apr 2020 23:59:58: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:00:06: 1000000 INFO @ Thu, 16 Apr 2020 00:00:16: 2000000 INFO @ Thu, 16 Apr 2020 00:00:24: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:00:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619549/SRX6619549.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619549/SRX6619549.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619549/SRX6619549.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619549/SRX6619549.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:00:28: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:00:28: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:00:33: 4000000 INFO @ Thu, 16 Apr 2020 00:00:37: 1000000 INFO @ Thu, 16 Apr 2020 00:00:42: 5000000 INFO @ Thu, 16 Apr 2020 00:00:47: 2000000 INFO @ Thu, 16 Apr 2020 00:00:51: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:00:56: 3000000 INFO @ Thu, 16 Apr 2020 00:00:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619549/SRX6619549.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619549/SRX6619549.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619549/SRX6619549.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619549/SRX6619549.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:00:58: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:00:58: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:01:01: 7000000 INFO @ Thu, 16 Apr 2020 00:01:06: 4000000 INFO @ Thu, 16 Apr 2020 00:01:08: 1000000 INFO @ Thu, 16 Apr 2020 00:01:10: 8000000 INFO @ Thu, 16 Apr 2020 00:01:16: 5000000 INFO @ Thu, 16 Apr 2020 00:01:19: 2000000 INFO @ Thu, 16 Apr 2020 00:01:19: 9000000 INFO @ Thu, 16 Apr 2020 00:01:27: 6000000 INFO @ Thu, 16 Apr 2020 00:01:29: 10000000 INFO @ Thu, 16 Apr 2020 00:01:29: 3000000 INFO @ Thu, 16 Apr 2020 00:01:37: 7000000 INFO @ Thu, 16 Apr 2020 00:01:38: 11000000 INFO @ Thu, 16 Apr 2020 00:01:40: 4000000 INFO @ Thu, 16 Apr 2020 00:01:47: 12000000 INFO @ Thu, 16 Apr 2020 00:01:48: 8000000 INFO @ Thu, 16 Apr 2020 00:01:51: 5000000 INFO @ Thu, 16 Apr 2020 00:01:57: 13000000 INFO @ Thu, 16 Apr 2020 00:01:58: 9000000 INFO @ Thu, 16 Apr 2020 00:02:01: 6000000 INFO @ Thu, 16 Apr 2020 00:02:07: 14000000 INFO @ Thu, 16 Apr 2020 00:02:08: 10000000 INFO @ Thu, 16 Apr 2020 00:02:11: 7000000 INFO @ Thu, 16 Apr 2020 00:02:16: 15000000 INFO @ Thu, 16 Apr 2020 00:02:18: 11000000 INFO @ Thu, 16 Apr 2020 00:02:21: 8000000 INFO @ Thu, 16 Apr 2020 00:02:25: 16000000 INFO @ Thu, 16 Apr 2020 00:02:29: 12000000 INFO @ Thu, 16 Apr 2020 00:02:32: 9000000 INFO @ Thu, 16 Apr 2020 00:02:35: 17000000 INFO @ Thu, 16 Apr 2020 00:02:39: 13000000 INFO @ Thu, 16 Apr 2020 00:02:43: 10000000 INFO @ Thu, 16 Apr 2020 00:02:44: 18000000 INFO @ Thu, 16 Apr 2020 00:02:50: 14000000 INFO @ Thu, 16 Apr 2020 00:02:53: 19000000 INFO @ Thu, 16 Apr 2020 00:02:53: 11000000 INFO @ Thu, 16 Apr 2020 00:03:01: 15000000 INFO @ Thu, 16 Apr 2020 00:03:03: 20000000 INFO @ Thu, 16 Apr 2020 00:03:04: 12000000 INFO @ Thu, 16 Apr 2020 00:03:11: 16000000 INFO @ Thu, 16 Apr 2020 00:03:12: 21000000 INFO @ Thu, 16 Apr 2020 00:03:15: 13000000 INFO @ Thu, 16 Apr 2020 00:03:21: 17000000 INFO @ Thu, 16 Apr 2020 00:03:22: 22000000 INFO @ Thu, 16 Apr 2020 00:03:25: 14000000 INFO @ Thu, 16 Apr 2020 00:03:31: 23000000 INFO @ Thu, 16 Apr 2020 00:03:32: 18000000 INFO @ Thu, 16 Apr 2020 00:03:36: 15000000 INFO @ Thu, 16 Apr 2020 00:03:40: 24000000 INFO @ Thu, 16 Apr 2020 00:03:42: 19000000 INFO @ Thu, 16 Apr 2020 00:03:46: 16000000 INFO @ Thu, 16 Apr 2020 00:03:50: 25000000 INFO @ Thu, 16 Apr 2020 00:03:52: 20000000 INFO @ Thu, 16 Apr 2020 00:03:56: 17000000 INFO @ Thu, 16 Apr 2020 00:03:59: 26000000 INFO @ Thu, 16 Apr 2020 00:04:03: 21000000 INFO @ Thu, 16 Apr 2020 00:04:07: 18000000 INFO @ Thu, 16 Apr 2020 00:04:08: 27000000 INFO @ Thu, 16 Apr 2020 00:04:13: 22000000 INFO @ Thu, 16 Apr 2020 00:04:18: 19000000 INFO @ Thu, 16 Apr 2020 00:04:19: 28000000 INFO @ Thu, 16 Apr 2020 00:04:23: 23000000 INFO @ Thu, 16 Apr 2020 00:04:28: 29000000 INFO @ Thu, 16 Apr 2020 00:04:28: 20000000 INFO @ Thu, 16 Apr 2020 00:04:33: 24000000 INFO @ Thu, 16 Apr 2020 00:04:37: 30000000 INFO @ Thu, 16 Apr 2020 00:04:38: 21000000 INFO @ Thu, 16 Apr 2020 00:04:43: 25000000 INFO @ Thu, 16 Apr 2020 00:04:47: 31000000 INFO @ Thu, 16 Apr 2020 00:04:49: 22000000 INFO @ Thu, 16 Apr 2020 00:04:55: 26000000 INFO @ Thu, 16 Apr 2020 00:04:57: 32000000 INFO @ Thu, 16 Apr 2020 00:05:00: 23000000 INFO @ Thu, 16 Apr 2020 00:05:05: 27000000 INFO @ Thu, 16 Apr 2020 00:05:06: 33000000 INFO @ Thu, 16 Apr 2020 00:05:11: 24000000 INFO @ Thu, 16 Apr 2020 00:05:15: 34000000 INFO @ Thu, 16 Apr 2020 00:05:16: 28000000 INFO @ Thu, 16 Apr 2020 00:05:22: 25000000 INFO @ Thu, 16 Apr 2020 00:05:24: 35000000 INFO @ Thu, 16 Apr 2020 00:05:27: 29000000 INFO @ Thu, 16 Apr 2020 00:05:32: 26000000 INFO @ Thu, 16 Apr 2020 00:05:33: 36000000 INFO @ Thu, 16 Apr 2020 00:05:38: 30000000 INFO @ Thu, 16 Apr 2020 00:05:43: 37000000 INFO @ Thu, 16 Apr 2020 00:05:44: 27000000 INFO @ Thu, 16 Apr 2020 00:05:49: 31000000 INFO @ Thu, 16 Apr 2020 00:05:52: 38000000 INFO @ Thu, 16 Apr 2020 00:05:54: 28000000 INFO @ Thu, 16 Apr 2020 00:05:59: 32000000 INFO @ Thu, 16 Apr 2020 00:06:01: 39000000 INFO @ Thu, 16 Apr 2020 00:06:05: 29000000 INFO @ Thu, 16 Apr 2020 00:06:11: 33000000 INFO @ Thu, 16 Apr 2020 00:06:11: 40000000 INFO @ Thu, 16 Apr 2020 00:06:16: 30000000 INFO @ Thu, 16 Apr 2020 00:06:20: 41000000 INFO @ Thu, 16 Apr 2020 00:06:22: 34000000 INFO @ Thu, 16 Apr 2020 00:06:27: 31000000 INFO @ Thu, 16 Apr 2020 00:06:29: 42000000 INFO @ Thu, 16 Apr 2020 00:06:32: 35000000 INFO @ Thu, 16 Apr 2020 00:06:38: 43000000 INFO @ Thu, 16 Apr 2020 00:06:39: 32000000 INFO @ Thu, 16 Apr 2020 00:06:43: 36000000 INFO @ Thu, 16 Apr 2020 00:06:47: 44000000 INFO @ Thu, 16 Apr 2020 00:06:50: 33000000 INFO @ Thu, 16 Apr 2020 00:06:54: 37000000 INFO @ Thu, 16 Apr 2020 00:06:56: 45000000 INFO @ Thu, 16 Apr 2020 00:07:00: 34000000 INFO @ Thu, 16 Apr 2020 00:07:06: 38000000 INFO @ Thu, 16 Apr 2020 00:07:06: 46000000 INFO @ Thu, 16 Apr 2020 00:07:10: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:07:10: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:07:10: #1 total tags in treatment: 14933150 INFO @ Thu, 16 Apr 2020 00:07:10: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:07:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:07:10: #1 tags after filtering in treatment: 12895784 INFO @ Thu, 16 Apr 2020 00:07:10: #1 Redundant rate of treatment: 0.14 INFO @ Thu, 16 Apr 2020 00:07:10: #1 finished! INFO @ Thu, 16 Apr 2020 00:07:10: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:07:10: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:07:11: #2 number of paired peaks: 259 WARNING @ Thu, 16 Apr 2020 00:07:11: Fewer paired peaks (259) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 259 pairs to build model! INFO @ Thu, 16 Apr 2020 00:07:11: start model_add_line... INFO @ Thu, 16 Apr 2020 00:07:11: start X-correlation... INFO @ Thu, 16 Apr 2020 00:07:11: end of X-cor INFO @ Thu, 16 Apr 2020 00:07:11: #2 finished! INFO @ Thu, 16 Apr 2020 00:07:11: #2 predicted fragment length is 201 bps INFO @ Thu, 16 Apr 2020 00:07:12: #2 alternative fragment length(s) may be 201 bps INFO @ Thu, 16 Apr 2020 00:07:12: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619549/SRX6619549.05_model.r WARNING @ Thu, 16 Apr 2020 00:07:12: #2 Since the d (201) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:07:12: #2 You may need to consider one of the other alternative d(s): 201 WARNING @ Thu, 16 Apr 2020 00:07:12: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:07:12: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:07:12: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:07:12: 35000000 INFO @ Thu, 16 Apr 2020 00:07:16: 39000000 INFO @ Thu, 16 Apr 2020 00:07:22: 36000000 INFO @ Thu, 16 Apr 2020 00:07:26: 40000000 INFO @ Thu, 16 Apr 2020 00:07:32: 37000000 INFO @ Thu, 16 Apr 2020 00:07:35: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:07:37: 41000000 INFO @ Thu, 16 Apr 2020 00:07:43: 38000000 INFO @ Thu, 16 Apr 2020 00:07:47: 42000000 INFO @ Thu, 16 Apr 2020 00:07:48: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619549/SRX6619549.05_peaks.xls INFO @ Thu, 16 Apr 2020 00:07:48: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619549/SRX6619549.05_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:07:48: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619549/SRX6619549.05_summits.bed INFO @ Thu, 16 Apr 2020 00:07:48: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (414 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:07:53: 39000000 INFO @ Thu, 16 Apr 2020 00:07:57: 43000000 INFO @ Thu, 16 Apr 2020 00:08:04: 40000000 INFO @ Thu, 16 Apr 2020 00:08:08: 44000000 INFO @ Thu, 16 Apr 2020 00:08:13: 41000000 INFO @ Thu, 16 Apr 2020 00:08:18: 45000000 INFO @ Thu, 16 Apr 2020 00:08:24: 42000000 INFO @ Thu, 16 Apr 2020 00:08:28: 46000000 INFO @ Thu, 16 Apr 2020 00:08:32: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:08:32: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:08:32: #1 total tags in treatment: 14933150 INFO @ Thu, 16 Apr 2020 00:08:32: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:08:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:08:33: #1 tags after filtering in treatment: 12895784 INFO @ Thu, 16 Apr 2020 00:08:33: #1 Redundant rate of treatment: 0.14 INFO @ Thu, 16 Apr 2020 00:08:33: #1 finished! INFO @ Thu, 16 Apr 2020 00:08:33: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:08:33: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:08:33: #2 number of paired peaks: 259 WARNING @ Thu, 16 Apr 2020 00:08:33: Fewer paired peaks (259) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 259 pairs to build model! INFO @ Thu, 16 Apr 2020 00:08:33: start model_add_line... INFO @ Thu, 16 Apr 2020 00:08:33: 43000000 INFO @ Thu, 16 Apr 2020 00:08:34: start X-correlation... INFO @ Thu, 16 Apr 2020 00:08:34: end of X-cor INFO @ Thu, 16 Apr 2020 00:08:34: #2 finished! INFO @ Thu, 16 Apr 2020 00:08:34: #2 predicted fragment length is 201 bps INFO @ Thu, 16 Apr 2020 00:08:34: #2 alternative fragment length(s) may be 201 bps INFO @ Thu, 16 Apr 2020 00:08:34: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619549/SRX6619549.10_model.r WARNING @ Thu, 16 Apr 2020 00:08:34: #2 Since the d (201) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:08:34: #2 You may need to consider one of the other alternative d(s): 201 WARNING @ Thu, 16 Apr 2020 00:08:34: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:08:34: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:08:34: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:08:42: 44000000 INFO @ Thu, 16 Apr 2020 00:08:51: 45000000 INFO @ Thu, 16 Apr 2020 00:08:57: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:09:00: 46000000 INFO @ Thu, 16 Apr 2020 00:09:04: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:09:04: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:09:04: #1 total tags in treatment: 14933150 INFO @ Thu, 16 Apr 2020 00:09:04: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:09:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:09:04: #1 tags after filtering in treatment: 12895784 INFO @ Thu, 16 Apr 2020 00:09:04: #1 Redundant rate of treatment: 0.14 INFO @ Thu, 16 Apr 2020 00:09:04: #1 finished! INFO @ Thu, 16 Apr 2020 00:09:04: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:09:04: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:09:05: #2 number of paired peaks: 259 WARNING @ Thu, 16 Apr 2020 00:09:05: Fewer paired peaks (259) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 259 pairs to build model! INFO @ Thu, 16 Apr 2020 00:09:05: start model_add_line... INFO @ Thu, 16 Apr 2020 00:09:05: start X-correlation... INFO @ Thu, 16 Apr 2020 00:09:05: end of X-cor INFO @ Thu, 16 Apr 2020 00:09:05: #2 finished! INFO @ Thu, 16 Apr 2020 00:09:05: #2 predicted fragment length is 201 bps INFO @ Thu, 16 Apr 2020 00:09:05: #2 alternative fragment length(s) may be 201 bps INFO @ Thu, 16 Apr 2020 00:09:05: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619549/SRX6619549.20_model.r WARNING @ Thu, 16 Apr 2020 00:09:05: #2 Since the d (201) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:09:05: #2 You may need to consider one of the other alternative d(s): 201 WARNING @ Thu, 16 Apr 2020 00:09:05: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:09:05: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:09:05: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:09:10: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619549/SRX6619549.10_peaks.xls INFO @ Thu, 16 Apr 2020 00:09:10: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619549/SRX6619549.10_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:09:10: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619549/SRX6619549.10_summits.bed INFO @ Thu, 16 Apr 2020 00:09:10: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (307 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:09:29: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:09:41: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619549/SRX6619549.20_peaks.xls INFO @ Thu, 16 Apr 2020 00:09:41: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619549/SRX6619549.20_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:09:41: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619549/SRX6619549.20_summits.bed INFO @ Thu, 16 Apr 2020 00:09:41: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (214 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。