Job ID = 5720166 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-04-15T12:34:45 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:34:45 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:34:45 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:34:45 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:34:45 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:34:45 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:52:13 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:52:13 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:09:28 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 39,751,115 reads read : 79,502,230 reads written : 79,502,230 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:09:42 39751115 reads; of these: 39751115 (100.00%) were paired; of these: 24879585 (62.59%) aligned concordantly 0 times 12264385 (30.85%) aligned concordantly exactly 1 time 2607145 (6.56%) aligned concordantly >1 times ---- 24879585 pairs aligned concordantly 0 times; of these: 12310378 (49.48%) aligned discordantly 1 time ---- 12569207 pairs aligned 0 times concordantly or discordantly; of these: 25138414 mates make up the pairs; of these: 21858403 (86.95%) aligned 0 times 1138499 (4.53%) aligned exactly 1 time 2141512 (8.52%) aligned >1 times 72.51% overall alignment rate Time searching: 01:09:42 Overall time: 01:09:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 44 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 4154003 / 26637523 = 0.1559 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:04:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619537/SRX6619537.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619537/SRX6619537.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619537/SRX6619537.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619537/SRX6619537.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:04:13: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:04:13: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:04:24: 1000000 INFO @ Thu, 16 Apr 2020 00:04:35: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:04:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619537/SRX6619537.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619537/SRX6619537.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619537/SRX6619537.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619537/SRX6619537.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:04:44: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:04:44: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:04:46: 3000000 INFO @ Thu, 16 Apr 2020 00:04:56: 1000000 INFO @ Thu, 16 Apr 2020 00:04:58: 4000000 INFO @ Thu, 16 Apr 2020 00:05:07: 2000000 INFO @ Thu, 16 Apr 2020 00:05:10: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:05:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619537/SRX6619537.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619537/SRX6619537.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619537/SRX6619537.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619537/SRX6619537.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:05:13: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:05:13: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:05:20: 3000000 INFO @ Thu, 16 Apr 2020 00:05:22: 6000000 INFO @ Thu, 16 Apr 2020 00:05:26: 1000000 INFO @ Thu, 16 Apr 2020 00:05:32: 4000000 INFO @ Thu, 16 Apr 2020 00:05:35: 7000000 INFO @ Thu, 16 Apr 2020 00:05:38: 2000000 INFO @ Thu, 16 Apr 2020 00:05:44: 5000000 INFO @ Thu, 16 Apr 2020 00:05:47: 8000000 INFO @ Thu, 16 Apr 2020 00:05:50: 3000000 INFO @ Thu, 16 Apr 2020 00:05:57: 6000000 INFO @ Thu, 16 Apr 2020 00:05:59: 9000000 INFO @ Thu, 16 Apr 2020 00:06:02: 4000000 INFO @ Thu, 16 Apr 2020 00:06:09: 7000000 INFO @ Thu, 16 Apr 2020 00:06:12: 10000000 INFO @ Thu, 16 Apr 2020 00:06:14: 5000000 INFO @ Thu, 16 Apr 2020 00:06:22: 8000000 INFO @ Thu, 16 Apr 2020 00:06:23: 11000000 INFO @ Thu, 16 Apr 2020 00:06:27: 6000000 INFO @ Thu, 16 Apr 2020 00:06:35: 9000000 INFO @ Thu, 16 Apr 2020 00:06:35: 12000000 INFO @ Thu, 16 Apr 2020 00:06:39: 7000000 INFO @ Thu, 16 Apr 2020 00:06:47: 10000000 INFO @ Thu, 16 Apr 2020 00:06:48: 13000000 INFO @ Thu, 16 Apr 2020 00:06:51: 8000000 INFO @ Thu, 16 Apr 2020 00:07:00: 11000000 INFO @ Thu, 16 Apr 2020 00:07:00: 14000000 INFO @ Thu, 16 Apr 2020 00:07:03: 9000000 INFO @ Thu, 16 Apr 2020 00:07:12: 12000000 INFO @ Thu, 16 Apr 2020 00:07:12: 15000000 INFO @ Thu, 16 Apr 2020 00:07:15: 10000000 INFO @ Thu, 16 Apr 2020 00:07:24: 13000000 INFO @ Thu, 16 Apr 2020 00:07:25: 16000000 INFO @ Thu, 16 Apr 2020 00:07:27: 11000000 INFO @ Thu, 16 Apr 2020 00:07:37: 14000000 INFO @ Thu, 16 Apr 2020 00:07:37: 17000000 INFO @ Thu, 16 Apr 2020 00:07:39: 12000000 INFO @ Thu, 16 Apr 2020 00:07:49: 18000000 INFO @ Thu, 16 Apr 2020 00:07:49: 15000000 INFO @ Thu, 16 Apr 2020 00:07:52: 13000000 INFO @ Thu, 16 Apr 2020 00:08:02: 19000000 INFO @ Thu, 16 Apr 2020 00:08:02: 16000000 INFO @ Thu, 16 Apr 2020 00:08:04: 14000000 INFO @ Thu, 16 Apr 2020 00:08:14: 20000000 INFO @ Thu, 16 Apr 2020 00:08:14: 17000000 INFO @ Thu, 16 Apr 2020 00:08:16: 15000000 INFO @ Thu, 16 Apr 2020 00:08:26: 21000000 INFO @ Thu, 16 Apr 2020 00:08:27: 18000000 INFO @ Thu, 16 Apr 2020 00:08:29: 16000000 INFO @ Thu, 16 Apr 2020 00:08:39: 22000000 INFO @ Thu, 16 Apr 2020 00:08:39: 19000000 INFO @ Thu, 16 Apr 2020 00:08:41: 17000000 INFO @ Thu, 16 Apr 2020 00:08:51: 23000000 INFO @ Thu, 16 Apr 2020 00:08:52: 20000000 INFO @ Thu, 16 Apr 2020 00:08:53: 18000000 INFO @ Thu, 16 Apr 2020 00:09:04: 24000000 INFO @ Thu, 16 Apr 2020 00:09:04: 21000000 INFO @ Thu, 16 Apr 2020 00:09:05: 19000000 INFO @ Thu, 16 Apr 2020 00:09:17: 20000000 INFO @ Thu, 16 Apr 2020 00:09:17: 22000000 INFO @ Thu, 16 Apr 2020 00:09:17: 25000000 INFO @ Thu, 16 Apr 2020 00:09:28: 21000000 INFO @ Thu, 16 Apr 2020 00:09:29: 23000000 INFO @ Thu, 16 Apr 2020 00:09:30: 26000000 INFO @ Thu, 16 Apr 2020 00:09:40: 22000000 INFO @ Thu, 16 Apr 2020 00:09:42: 27000000 INFO @ Thu, 16 Apr 2020 00:09:42: 24000000 INFO @ Thu, 16 Apr 2020 00:09:52: 23000000 INFO @ Thu, 16 Apr 2020 00:09:54: 28000000 INFO @ Thu, 16 Apr 2020 00:09:55: 25000000 INFO @ Thu, 16 Apr 2020 00:10:05: 24000000 INFO @ Thu, 16 Apr 2020 00:10:06: 29000000 INFO @ Thu, 16 Apr 2020 00:10:07: 26000000 INFO @ Thu, 16 Apr 2020 00:10:17: 25000000 INFO @ Thu, 16 Apr 2020 00:10:17: 30000000 INFO @ Thu, 16 Apr 2020 00:10:20: 27000000 INFO @ Thu, 16 Apr 2020 00:10:29: 31000000 INFO @ Thu, 16 Apr 2020 00:10:29: 26000000 INFO @ Thu, 16 Apr 2020 00:10:32: 28000000 INFO @ Thu, 16 Apr 2020 00:10:41: 32000000 INFO @ Thu, 16 Apr 2020 00:10:41: 27000000 INFO @ Thu, 16 Apr 2020 00:10:45: 29000000 INFO @ Thu, 16 Apr 2020 00:10:52: 33000000 INFO @ Thu, 16 Apr 2020 00:10:53: 28000000 INFO @ Thu, 16 Apr 2020 00:10:57: 30000000 INFO @ Thu, 16 Apr 2020 00:11:04: 34000000 INFO @ Thu, 16 Apr 2020 00:11:05: 29000000 INFO @ Thu, 16 Apr 2020 00:11:09: 31000000 INFO @ Thu, 16 Apr 2020 00:11:16: 35000000 INFO @ Thu, 16 Apr 2020 00:11:17: 30000000 INFO @ Thu, 16 Apr 2020 00:11:22: 32000000 INFO @ Thu, 16 Apr 2020 00:11:28: 36000000 INFO @ Thu, 16 Apr 2020 00:11:29: 31000000 INFO @ Thu, 16 Apr 2020 00:11:34: 33000000 INFO @ Thu, 16 Apr 2020 00:11:40: 37000000 INFO @ Thu, 16 Apr 2020 00:11:41: 32000000 INFO @ Thu, 16 Apr 2020 00:11:46: 34000000 INFO @ Thu, 16 Apr 2020 00:11:51: 38000000 INFO @ Thu, 16 Apr 2020 00:11:52: 33000000 INFO @ Thu, 16 Apr 2020 00:11:59: 35000000 INFO @ Thu, 16 Apr 2020 00:12:03: 39000000 INFO @ Thu, 16 Apr 2020 00:12:04: 34000000 INFO @ Thu, 16 Apr 2020 00:12:11: 36000000 INFO @ Thu, 16 Apr 2020 00:12:14: 40000000 INFO @ Thu, 16 Apr 2020 00:12:17: 35000000 INFO @ Thu, 16 Apr 2020 00:12:23: 37000000 INFO @ Thu, 16 Apr 2020 00:12:26: 41000000 INFO @ Thu, 16 Apr 2020 00:12:29: 36000000 INFO @ Thu, 16 Apr 2020 00:12:35: 38000000 INFO @ Thu, 16 Apr 2020 00:12:37: 42000000 INFO @ Thu, 16 Apr 2020 00:12:40: 37000000 INFO @ Thu, 16 Apr 2020 00:12:47: 39000000 INFO @ Thu, 16 Apr 2020 00:12:49: 43000000 INFO @ Thu, 16 Apr 2020 00:12:52: 38000000 INFO @ Thu, 16 Apr 2020 00:12:59: 40000000 INFO @ Thu, 16 Apr 2020 00:13:00: 44000000 INFO @ Thu, 16 Apr 2020 00:13:04: 39000000 INFO @ Thu, 16 Apr 2020 00:13:11: 41000000 INFO @ Thu, 16 Apr 2020 00:13:12: 45000000 INFO @ Thu, 16 Apr 2020 00:13:16: 40000000 INFO @ Thu, 16 Apr 2020 00:13:22: 42000000 INFO @ Thu, 16 Apr 2020 00:13:23: 46000000 INFO @ Thu, 16 Apr 2020 00:13:27: 41000000 INFO @ Thu, 16 Apr 2020 00:13:34: 43000000 INFO @ Thu, 16 Apr 2020 00:13:35: 47000000 INFO @ Thu, 16 Apr 2020 00:13:39: 42000000 INFO @ Thu, 16 Apr 2020 00:13:45: 44000000 INFO @ Thu, 16 Apr 2020 00:13:46: 48000000 INFO @ Thu, 16 Apr 2020 00:13:51: 43000000 INFO @ Thu, 16 Apr 2020 00:13:57: 45000000 INFO @ Thu, 16 Apr 2020 00:13:58: 49000000 INFO @ Thu, 16 Apr 2020 00:14:02: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:14:02: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:14:02: #1 total tags in treatment: 12357873 INFO @ Thu, 16 Apr 2020 00:14:02: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:14:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:14:02: #1 tags after filtering in treatment: 10443135 INFO @ Thu, 16 Apr 2020 00:14:02: #1 Redundant rate of treatment: 0.15 INFO @ Thu, 16 Apr 2020 00:14:02: #1 finished! INFO @ Thu, 16 Apr 2020 00:14:02: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:14:02: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:14:03: 44000000 INFO @ Thu, 16 Apr 2020 00:14:03: #2 number of paired peaks: 308 WARNING @ Thu, 16 Apr 2020 00:14:03: Fewer paired peaks (308) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 308 pairs to build model! INFO @ Thu, 16 Apr 2020 00:14:03: start model_add_line... INFO @ Thu, 16 Apr 2020 00:14:03: start X-correlation... INFO @ Thu, 16 Apr 2020 00:14:03: end of X-cor INFO @ Thu, 16 Apr 2020 00:14:03: #2 finished! INFO @ Thu, 16 Apr 2020 00:14:03: #2 predicted fragment length is 218 bps INFO @ Thu, 16 Apr 2020 00:14:03: #2 alternative fragment length(s) may be 218 bps INFO @ Thu, 16 Apr 2020 00:14:03: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619537/SRX6619537.05_model.r WARNING @ Thu, 16 Apr 2020 00:14:03: #2 Since the d (218) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:14:03: #2 You may need to consider one of the other alternative d(s): 218 WARNING @ Thu, 16 Apr 2020 00:14:03: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:14:03: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:14:03: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:14:08: 46000000 INFO @ Thu, 16 Apr 2020 00:14:14: 45000000 INFO @ Thu, 16 Apr 2020 00:14:19: 47000000 INFO @ Thu, 16 Apr 2020 00:14:25: 46000000 INFO @ Thu, 16 Apr 2020 00:14:26: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:14:31: 48000000 INFO @ Thu, 16 Apr 2020 00:14:37: 47000000 INFO @ Thu, 16 Apr 2020 00:14:37: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619537/SRX6619537.05_peaks.xls INFO @ Thu, 16 Apr 2020 00:14:37: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619537/SRX6619537.05_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:14:37: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619537/SRX6619537.05_summits.bed INFO @ Thu, 16 Apr 2020 00:14:37: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (513 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 16 Apr 2020 00:14:42: 49000000 INFO @ Thu, 16 Apr 2020 00:14:45: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:14:45: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:14:45: #1 total tags in treatment: 12357873 INFO @ Thu, 16 Apr 2020 00:14:45: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:14:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:14:46: #1 tags after filtering in treatment: 10443135 INFO @ Thu, 16 Apr 2020 00:14:46: #1 Redundant rate of treatment: 0.15 INFO @ Thu, 16 Apr 2020 00:14:46: #1 finished! INFO @ Thu, 16 Apr 2020 00:14:46: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:14:46: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:14:46: #2 number of paired peaks: 308 WARNING @ Thu, 16 Apr 2020 00:14:46: Fewer paired peaks (308) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 308 pairs to build model! INFO @ Thu, 16 Apr 2020 00:14:46: start model_add_line... INFO @ Thu, 16 Apr 2020 00:14:46: start X-correlation... INFO @ Thu, 16 Apr 2020 00:14:46: end of X-cor INFO @ Thu, 16 Apr 2020 00:14:46: #2 finished! INFO @ Thu, 16 Apr 2020 00:14:46: #2 predicted fragment length is 218 bps INFO @ Thu, 16 Apr 2020 00:14:46: #2 alternative fragment length(s) may be 218 bps INFO @ Thu, 16 Apr 2020 00:14:46: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619537/SRX6619537.10_model.r WARNING @ Thu, 16 Apr 2020 00:14:46: #2 Since the d (218) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:14:46: #2 You may need to consider one of the other alternative d(s): 218 WARNING @ Thu, 16 Apr 2020 00:14:46: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:14:46: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:14:46: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:14:48: 48000000 INFO @ Thu, 16 Apr 2020 00:14:58: 49000000 INFO @ Thu, 16 Apr 2020 00:15:02: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:15:02: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:15:02: #1 total tags in treatment: 12357873 INFO @ Thu, 16 Apr 2020 00:15:02: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:15:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:15:02: #1 tags after filtering in treatment: 10443135 INFO @ Thu, 16 Apr 2020 00:15:02: #1 Redundant rate of treatment: 0.15 INFO @ Thu, 16 Apr 2020 00:15:02: #1 finished! INFO @ Thu, 16 Apr 2020 00:15:02: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:15:02: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:15:03: #2 number of paired peaks: 308 WARNING @ Thu, 16 Apr 2020 00:15:03: Fewer paired peaks (308) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 308 pairs to build model! INFO @ Thu, 16 Apr 2020 00:15:03: start model_add_line... INFO @ Thu, 16 Apr 2020 00:15:03: start X-correlation... INFO @ Thu, 16 Apr 2020 00:15:03: end of X-cor INFO @ Thu, 16 Apr 2020 00:15:03: #2 finished! INFO @ Thu, 16 Apr 2020 00:15:03: #2 predicted fragment length is 218 bps INFO @ Thu, 16 Apr 2020 00:15:03: #2 alternative fragment length(s) may be 218 bps INFO @ Thu, 16 Apr 2020 00:15:03: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619537/SRX6619537.20_model.r WARNING @ Thu, 16 Apr 2020 00:15:03: #2 Since the d (218) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:15:03: #2 You may need to consider one of the other alternative d(s): 218 WARNING @ Thu, 16 Apr 2020 00:15:03: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:15:03: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:15:03: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:15:09: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:15:19: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619537/SRX6619537.10_peaks.xls INFO @ Thu, 16 Apr 2020 00:15:19: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619537/SRX6619537.10_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:15:19: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619537/SRX6619537.10_summits.bed INFO @ Thu, 16 Apr 2020 00:15:19: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (343 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:15:25: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:15:35: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619537/SRX6619537.20_peaks.xls INFO @ Thu, 16 Apr 2020 00:15:35: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619537/SRX6619537.20_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:15:35: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619537/SRX6619537.20_summits.bed INFO @ Thu, 16 Apr 2020 00:15:35: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (214 records, 4 fields): 2 millis CompletedMACS2peakCalling BigWig に変換しました。