Job ID = 5720163 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-04-15T12:50:31 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:52:13 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:53:19 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:01:54 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:02:28 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:02:28 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '165.112.9.238' from '172.19.7.199' 2020-04-15T13:02:28 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (165.112.9.238) from '172.19.7.199' 2020-04-15T13:02:55 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:05:33 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:15:39 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 36,791,139 reads read : 73,582,278 reads written : 73,582,278 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:25:49 36791139 reads; of these: 36791139 (100.00%) were paired; of these: 17951494 (48.79%) aligned concordantly 0 times 13662235 (37.13%) aligned concordantly exactly 1 time 5177410 (14.07%) aligned concordantly >1 times ---- 17951494 pairs aligned concordantly 0 times; of these: 7441676 (41.45%) aligned discordantly 1 time ---- 10509818 pairs aligned 0 times concordantly or discordantly; of these: 21019636 mates make up the pairs; of these: 17142206 (81.55%) aligned 0 times 1307714 (6.22%) aligned exactly 1 time 2569716 (12.23%) aligned >1 times 76.70% overall alignment rate Time searching: 01:25:49 Overall time: 01:25:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 44 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 4475196 / 25915580 = 0.1727 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:19:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619534/SRX6619534.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619534/SRX6619534.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619534/SRX6619534.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619534/SRX6619534.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:19:45: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:19:45: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:19:53: 1000000 INFO @ Thu, 16 Apr 2020 00:20:02: 2000000 INFO @ Thu, 16 Apr 2020 00:20:10: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:20:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619534/SRX6619534.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619534/SRX6619534.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619534/SRX6619534.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619534/SRX6619534.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:20:15: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:20:15: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:20:19: 4000000 INFO @ Thu, 16 Apr 2020 00:20:24: 1000000 INFO @ Thu, 16 Apr 2020 00:20:29: 5000000 INFO @ Thu, 16 Apr 2020 00:20:33: 2000000 INFO @ Thu, 16 Apr 2020 00:20:38: 6000000 INFO @ Thu, 16 Apr 2020 00:20:43: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:20:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619534/SRX6619534.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619534/SRX6619534.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619534/SRX6619534.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619534/SRX6619534.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:20:45: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:20:45: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:20:47: 7000000 INFO @ Thu, 16 Apr 2020 00:20:52: 4000000 INFO @ Thu, 16 Apr 2020 00:20:55: 1000000 INFO @ Thu, 16 Apr 2020 00:20:57: 8000000 INFO @ Thu, 16 Apr 2020 00:21:01: 5000000 INFO @ Thu, 16 Apr 2020 00:21:05: 2000000 INFO @ Thu, 16 Apr 2020 00:21:06: 9000000 INFO @ Thu, 16 Apr 2020 00:21:11: 6000000 INFO @ Thu, 16 Apr 2020 00:21:14: 3000000 INFO @ Thu, 16 Apr 2020 00:21:16: 10000000 INFO @ Thu, 16 Apr 2020 00:21:20: 7000000 INFO @ Thu, 16 Apr 2020 00:21:24: 4000000 INFO @ Thu, 16 Apr 2020 00:21:26: 11000000 INFO @ Thu, 16 Apr 2020 00:21:29: 8000000 INFO @ Thu, 16 Apr 2020 00:21:34: 5000000 INFO @ Thu, 16 Apr 2020 00:21:36: 12000000 INFO @ Thu, 16 Apr 2020 00:21:38: 9000000 INFO @ Thu, 16 Apr 2020 00:21:44: 6000000 INFO @ Thu, 16 Apr 2020 00:21:46: 13000000 INFO @ Thu, 16 Apr 2020 00:21:48: 10000000 INFO @ Thu, 16 Apr 2020 00:21:54: 7000000 INFO @ Thu, 16 Apr 2020 00:21:56: 14000000 INFO @ Thu, 16 Apr 2020 00:21:57: 11000000 INFO @ Thu, 16 Apr 2020 00:22:04: 8000000 INFO @ Thu, 16 Apr 2020 00:22:06: 15000000 INFO @ Thu, 16 Apr 2020 00:22:06: 12000000 INFO @ Thu, 16 Apr 2020 00:22:14: 9000000 INFO @ Thu, 16 Apr 2020 00:22:16: 13000000 INFO @ Thu, 16 Apr 2020 00:22:16: 16000000 INFO @ Thu, 16 Apr 2020 00:22:24: 10000000 INFO @ Thu, 16 Apr 2020 00:22:25: 14000000 INFO @ Thu, 16 Apr 2020 00:22:26: 17000000 INFO @ Thu, 16 Apr 2020 00:22:34: 11000000 INFO @ Thu, 16 Apr 2020 00:22:35: 15000000 INFO @ Thu, 16 Apr 2020 00:22:36: 18000000 INFO @ Thu, 16 Apr 2020 00:22:44: 12000000 INFO @ Thu, 16 Apr 2020 00:22:45: 16000000 INFO @ Thu, 16 Apr 2020 00:22:46: 19000000 INFO @ Thu, 16 Apr 2020 00:22:54: 17000000 INFO @ Thu, 16 Apr 2020 00:22:54: 13000000 INFO @ Thu, 16 Apr 2020 00:22:56: 20000000 INFO @ Thu, 16 Apr 2020 00:23:03: 18000000 INFO @ Thu, 16 Apr 2020 00:23:04: 14000000 INFO @ Thu, 16 Apr 2020 00:23:06: 21000000 INFO @ Thu, 16 Apr 2020 00:23:13: 19000000 INFO @ Thu, 16 Apr 2020 00:23:15: 15000000 INFO @ Thu, 16 Apr 2020 00:23:16: 22000000 INFO @ Thu, 16 Apr 2020 00:23:22: 20000000 INFO @ Thu, 16 Apr 2020 00:23:25: 16000000 INFO @ Thu, 16 Apr 2020 00:23:26: 23000000 INFO @ Thu, 16 Apr 2020 00:23:33: 21000000 INFO @ Thu, 16 Apr 2020 00:23:35: 17000000 INFO @ Thu, 16 Apr 2020 00:23:35: 24000000 INFO @ Thu, 16 Apr 2020 00:23:42: 22000000 INFO @ Thu, 16 Apr 2020 00:23:45: 18000000 INFO @ Thu, 16 Apr 2020 00:23:45: 25000000 INFO @ Thu, 16 Apr 2020 00:23:52: 23000000 INFO @ Thu, 16 Apr 2020 00:23:54: 26000000 INFO @ Thu, 16 Apr 2020 00:23:54: 19000000 INFO @ Thu, 16 Apr 2020 00:24:02: 24000000 INFO @ Thu, 16 Apr 2020 00:24:03: 27000000 INFO @ Thu, 16 Apr 2020 00:24:04: 20000000 INFO @ Thu, 16 Apr 2020 00:24:11: 25000000 INFO @ Thu, 16 Apr 2020 00:24:13: 28000000 INFO @ Thu, 16 Apr 2020 00:24:14: 21000000 INFO @ Thu, 16 Apr 2020 00:24:20: 26000000 INFO @ Thu, 16 Apr 2020 00:24:22: 29000000 INFO @ Thu, 16 Apr 2020 00:24:24: 22000000 INFO @ Thu, 16 Apr 2020 00:24:30: 27000000 INFO @ Thu, 16 Apr 2020 00:24:31: 30000000 INFO @ Thu, 16 Apr 2020 00:24:33: 23000000 INFO @ Thu, 16 Apr 2020 00:24:39: 28000000 INFO @ Thu, 16 Apr 2020 00:24:40: 31000000 INFO @ Thu, 16 Apr 2020 00:24:43: 24000000 INFO @ Thu, 16 Apr 2020 00:24:48: 29000000 INFO @ Thu, 16 Apr 2020 00:24:50: 32000000 INFO @ Thu, 16 Apr 2020 00:24:52: 25000000 INFO @ Thu, 16 Apr 2020 00:24:58: 30000000 INFO @ Thu, 16 Apr 2020 00:24:59: 33000000 INFO @ Thu, 16 Apr 2020 00:25:01: 26000000 INFO @ Thu, 16 Apr 2020 00:25:07: 31000000 INFO @ Thu, 16 Apr 2020 00:25:08: 34000000 INFO @ Thu, 16 Apr 2020 00:25:10: 27000000 INFO @ Thu, 16 Apr 2020 00:25:16: 32000000 INFO @ Thu, 16 Apr 2020 00:25:17: 35000000 INFO @ Thu, 16 Apr 2020 00:25:19: 28000000 INFO @ Thu, 16 Apr 2020 00:25:25: 33000000 INFO @ Thu, 16 Apr 2020 00:25:26: 36000000 INFO @ Thu, 16 Apr 2020 00:25:28: 29000000 INFO @ Thu, 16 Apr 2020 00:25:35: 34000000 INFO @ Thu, 16 Apr 2020 00:25:36: 37000000 INFO @ Thu, 16 Apr 2020 00:25:37: 30000000 INFO @ Thu, 16 Apr 2020 00:25:44: 35000000 INFO @ Thu, 16 Apr 2020 00:25:45: 38000000 INFO @ Thu, 16 Apr 2020 00:25:46: 31000000 INFO @ Thu, 16 Apr 2020 00:25:53: 36000000 INFO @ Thu, 16 Apr 2020 00:25:54: 39000000 INFO @ Thu, 16 Apr 2020 00:25:55: 32000000 INFO @ Thu, 16 Apr 2020 00:26:02: 37000000 INFO @ Thu, 16 Apr 2020 00:26:03: 40000000 INFO @ Thu, 16 Apr 2020 00:26:04: 33000000 INFO @ Thu, 16 Apr 2020 00:26:11: 38000000 INFO @ Thu, 16 Apr 2020 00:26:12: 41000000 INFO @ Thu, 16 Apr 2020 00:26:13: 34000000 INFO @ Thu, 16 Apr 2020 00:26:20: 39000000 INFO @ Thu, 16 Apr 2020 00:26:22: 35000000 INFO @ Thu, 16 Apr 2020 00:26:22: 42000000 INFO @ Thu, 16 Apr 2020 00:26:30: 40000000 INFO @ Thu, 16 Apr 2020 00:26:31: 36000000 INFO @ Thu, 16 Apr 2020 00:26:32: 43000000 INFO @ Thu, 16 Apr 2020 00:26:39: 41000000 INFO @ Thu, 16 Apr 2020 00:26:40: 37000000 INFO @ Thu, 16 Apr 2020 00:26:41: 44000000 INFO @ Thu, 16 Apr 2020 00:26:49: 42000000 INFO @ Thu, 16 Apr 2020 00:26:49: 38000000 INFO @ Thu, 16 Apr 2020 00:26:51: 45000000 INFO @ Thu, 16 Apr 2020 00:26:58: 39000000 INFO @ Thu, 16 Apr 2020 00:26:58: 43000000 INFO @ Thu, 16 Apr 2020 00:27:01: 46000000 INFO @ Thu, 16 Apr 2020 00:27:07: 40000000 INFO @ Thu, 16 Apr 2020 00:27:08: 44000000 INFO @ Thu, 16 Apr 2020 00:27:10: 47000000 INFO @ Thu, 16 Apr 2020 00:27:15: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:27:15: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:27:15: #1 total tags in treatment: 15247673 INFO @ Thu, 16 Apr 2020 00:27:15: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:27:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:27:15: #1 tags after filtering in treatment: 11397753 INFO @ Thu, 16 Apr 2020 00:27:15: #1 Redundant rate of treatment: 0.25 INFO @ Thu, 16 Apr 2020 00:27:15: #1 finished! INFO @ Thu, 16 Apr 2020 00:27:15: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:27:15: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:27:16: #2 number of paired peaks: 466 WARNING @ Thu, 16 Apr 2020 00:27:16: Fewer paired peaks (466) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 466 pairs to build model! INFO @ Thu, 16 Apr 2020 00:27:16: start model_add_line... INFO @ Thu, 16 Apr 2020 00:27:16: start X-correlation... INFO @ Thu, 16 Apr 2020 00:27:16: end of X-cor INFO @ Thu, 16 Apr 2020 00:27:16: #2 finished! INFO @ Thu, 16 Apr 2020 00:27:16: #2 predicted fragment length is 221 bps INFO @ Thu, 16 Apr 2020 00:27:16: #2 alternative fragment length(s) may be 221 bps INFO @ Thu, 16 Apr 2020 00:27:16: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619534/SRX6619534.05_model.r WARNING @ Thu, 16 Apr 2020 00:27:16: #2 Since the d (221) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:27:16: #2 You may need to consider one of the other alternative d(s): 221 WARNING @ Thu, 16 Apr 2020 00:27:16: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:27:16: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:27:16: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:27:16: 41000000 INFO @ Thu, 16 Apr 2020 00:27:18: 45000000 INFO @ Thu, 16 Apr 2020 00:27:26: 42000000 INFO @ Thu, 16 Apr 2020 00:27:27: 46000000 INFO @ Thu, 16 Apr 2020 00:27:36: 43000000 INFO @ Thu, 16 Apr 2020 00:27:37: 47000000 INFO @ Thu, 16 Apr 2020 00:27:42: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:27:42: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:27:42: #1 total tags in treatment: 15247673 INFO @ Thu, 16 Apr 2020 00:27:42: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:27:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:27:42: #1 tags after filtering in treatment: 11397753 INFO @ Thu, 16 Apr 2020 00:27:42: #1 Redundant rate of treatment: 0.25 INFO @ Thu, 16 Apr 2020 00:27:42: #1 finished! INFO @ Thu, 16 Apr 2020 00:27:42: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:27:42: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:27:43: #2 number of paired peaks: 466 WARNING @ Thu, 16 Apr 2020 00:27:43: Fewer paired peaks (466) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 466 pairs to build model! INFO @ Thu, 16 Apr 2020 00:27:43: start model_add_line... INFO @ Thu, 16 Apr 2020 00:27:43: start X-correlation... INFO @ Thu, 16 Apr 2020 00:27:43: end of X-cor INFO @ Thu, 16 Apr 2020 00:27:43: #2 finished! INFO @ Thu, 16 Apr 2020 00:27:43: #2 predicted fragment length is 221 bps INFO @ Thu, 16 Apr 2020 00:27:43: #2 alternative fragment length(s) may be 221 bps INFO @ Thu, 16 Apr 2020 00:27:43: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619534/SRX6619534.10_model.r WARNING @ Thu, 16 Apr 2020 00:27:43: #2 Since the d (221) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:27:43: #2 You may need to consider one of the other alternative d(s): 221 WARNING @ Thu, 16 Apr 2020 00:27:43: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:27:43: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:27:43: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:27:45: 44000000 INFO @ Thu, 16 Apr 2020 00:27:46: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:27:54: 45000000 INFO @ Thu, 16 Apr 2020 00:28:00: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619534/SRX6619534.05_peaks.xls INFO @ Thu, 16 Apr 2020 00:28:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619534/SRX6619534.05_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:28:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619534/SRX6619534.05_summits.bed INFO @ Thu, 16 Apr 2020 00:28:00: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (1284 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:28:03: 46000000 INFO @ Thu, 16 Apr 2020 00:28:12: 47000000 INFO @ Thu, 16 Apr 2020 00:28:13: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:28:16: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:28:16: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:28:16: #1 total tags in treatment: 15247673 INFO @ Thu, 16 Apr 2020 00:28:16: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:28:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:28:17: #1 tags after filtering in treatment: 11397753 INFO @ Thu, 16 Apr 2020 00:28:17: #1 Redundant rate of treatment: 0.25 INFO @ Thu, 16 Apr 2020 00:28:17: #1 finished! INFO @ Thu, 16 Apr 2020 00:28:17: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:28:17: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:28:18: #2 number of paired peaks: 466 WARNING @ Thu, 16 Apr 2020 00:28:18: Fewer paired peaks (466) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 466 pairs to build model! INFO @ Thu, 16 Apr 2020 00:28:18: start model_add_line... INFO @ Thu, 16 Apr 2020 00:28:18: start X-correlation... INFO @ Thu, 16 Apr 2020 00:28:18: end of X-cor INFO @ Thu, 16 Apr 2020 00:28:18: #2 finished! INFO @ Thu, 16 Apr 2020 00:28:18: #2 predicted fragment length is 221 bps INFO @ Thu, 16 Apr 2020 00:28:18: #2 alternative fragment length(s) may be 221 bps INFO @ Thu, 16 Apr 2020 00:28:18: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619534/SRX6619534.20_model.r WARNING @ Thu, 16 Apr 2020 00:28:18: #2 Since the d (221) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:28:18: #2 You may need to consider one of the other alternative d(s): 221 WARNING @ Thu, 16 Apr 2020 00:28:18: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:28:18: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:28:18: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:28:26: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619534/SRX6619534.10_peaks.xls INFO @ Thu, 16 Apr 2020 00:28:26: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619534/SRX6619534.10_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:28:26: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619534/SRX6619534.10_summits.bed INFO @ Thu, 16 Apr 2020 00:28:26: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (634 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 16 Apr 2020 00:28:47: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:28:59: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619534/SRX6619534.20_peaks.xls INFO @ Thu, 16 Apr 2020 00:28:59: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619534/SRX6619534.20_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:28:59: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619534/SRX6619534.20_summits.bed INFO @ Thu, 16 Apr 2020 00:28:59: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (318 records, 4 fields): 1 millis CompletedMACS2peakCalling BigWig に変換しました。