Job ID = 5720161 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-04-15T12:53:19 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:05:33 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:15:27 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 36,263,918 reads read : 72,527,836 reads written : 72,527,836 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:33:56 36263918 reads; of these: 36263918 (100.00%) were paired; of these: 15959185 (44.01%) aligned concordantly 0 times 16929802 (46.68%) aligned concordantly exactly 1 time 3374931 (9.31%) aligned concordantly >1 times ---- 15959185 pairs aligned concordantly 0 times; of these: 9027148 (56.56%) aligned discordantly 1 time ---- 6932037 pairs aligned 0 times concordantly or discordantly; of these: 13864074 mates make up the pairs; of these: 10948670 (78.97%) aligned 0 times 1110362 (8.01%) aligned exactly 1 time 1805042 (13.02%) aligned >1 times 84.90% overall alignment rate Time searching: 01:33:56 Overall time: 01:33:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 48 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 3929552 / 28811810 = 0.1364 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:31:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619532/SRX6619532.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619532/SRX6619532.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619532/SRX6619532.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619532/SRX6619532.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:31:30: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:31:30: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:31:39: 1000000 INFO @ Thu, 16 Apr 2020 00:31:49: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:31:58: 3000000 INFO @ Thu, 16 Apr 2020 00:31:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619532/SRX6619532.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619532/SRX6619532.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619532/SRX6619532.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619532/SRX6619532.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:31:59: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:31:59: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:32:07: 4000000 INFO @ Thu, 16 Apr 2020 00:32:11: 1000000 INFO @ Thu, 16 Apr 2020 00:32:17: 5000000 INFO @ Thu, 16 Apr 2020 00:32:23: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:32:27: 6000000 INFO @ Thu, 16 Apr 2020 00:32:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619532/SRX6619532.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619532/SRX6619532.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619532/SRX6619532.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619532/SRX6619532.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:32:29: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:32:29: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:32:36: 3000000 INFO @ Thu, 16 Apr 2020 00:32:38: 7000000 INFO @ Thu, 16 Apr 2020 00:32:42: 1000000 INFO @ Thu, 16 Apr 2020 00:32:48: 8000000 INFO @ Thu, 16 Apr 2020 00:32:50: 4000000 INFO @ Thu, 16 Apr 2020 00:32:55: 2000000 INFO @ Thu, 16 Apr 2020 00:33:00: 9000000 INFO @ Thu, 16 Apr 2020 00:33:03: 5000000 INFO @ Thu, 16 Apr 2020 00:33:09: 3000000 INFO @ Thu, 16 Apr 2020 00:33:12: 10000000 INFO @ Thu, 16 Apr 2020 00:33:17: 6000000 INFO @ Thu, 16 Apr 2020 00:33:22: 4000000 INFO @ Thu, 16 Apr 2020 00:33:24: 11000000 INFO @ Thu, 16 Apr 2020 00:33:30: 7000000 INFO @ Thu, 16 Apr 2020 00:33:36: 5000000 INFO @ Thu, 16 Apr 2020 00:33:36: 12000000 INFO @ Thu, 16 Apr 2020 00:33:44: 8000000 INFO @ Thu, 16 Apr 2020 00:33:49: 13000000 INFO @ Thu, 16 Apr 2020 00:33:49: 6000000 INFO @ Thu, 16 Apr 2020 00:33:57: 9000000 INFO @ Thu, 16 Apr 2020 00:34:02: 14000000 INFO @ Thu, 16 Apr 2020 00:34:03: 7000000 INFO @ Thu, 16 Apr 2020 00:34:11: 10000000 INFO @ Thu, 16 Apr 2020 00:34:15: 15000000 INFO @ Thu, 16 Apr 2020 00:34:16: 8000000 INFO @ Thu, 16 Apr 2020 00:34:24: 11000000 INFO @ Thu, 16 Apr 2020 00:34:28: 16000000 INFO @ Thu, 16 Apr 2020 00:34:30: 9000000 INFO @ Thu, 16 Apr 2020 00:34:38: 12000000 INFO @ Thu, 16 Apr 2020 00:34:42: 17000000 INFO @ Thu, 16 Apr 2020 00:34:43: 10000000 INFO @ Thu, 16 Apr 2020 00:34:51: 13000000 INFO @ Thu, 16 Apr 2020 00:34:55: 18000000 INFO @ Thu, 16 Apr 2020 00:34:56: 11000000 INFO @ Thu, 16 Apr 2020 00:35:05: 14000000 INFO @ Thu, 16 Apr 2020 00:35:08: 19000000 INFO @ Thu, 16 Apr 2020 00:35:09: 12000000 INFO @ Thu, 16 Apr 2020 00:35:18: 15000000 INFO @ Thu, 16 Apr 2020 00:35:22: 20000000 INFO @ Thu, 16 Apr 2020 00:35:22: 13000000 INFO @ Thu, 16 Apr 2020 00:35:32: 16000000 INFO @ Thu, 16 Apr 2020 00:35:35: 21000000 INFO @ Thu, 16 Apr 2020 00:35:36: 14000000 INFO @ Thu, 16 Apr 2020 00:35:47: 17000000 INFO @ Thu, 16 Apr 2020 00:35:49: 22000000 INFO @ Thu, 16 Apr 2020 00:35:51: 15000000 INFO @ Thu, 16 Apr 2020 00:36:01: 18000000 INFO @ Thu, 16 Apr 2020 00:36:02: 23000000 INFO @ Thu, 16 Apr 2020 00:36:05: 16000000 INFO @ Thu, 16 Apr 2020 00:36:15: 24000000 INFO @ Thu, 16 Apr 2020 00:36:16: 19000000 INFO @ Thu, 16 Apr 2020 00:36:20: 17000000 INFO @ Thu, 16 Apr 2020 00:36:28: 25000000 INFO @ Thu, 16 Apr 2020 00:36:31: 20000000 INFO @ Thu, 16 Apr 2020 00:36:34: 18000000 INFO @ Thu, 16 Apr 2020 00:36:42: 26000000 INFO @ Thu, 16 Apr 2020 00:36:46: 21000000 INFO @ Thu, 16 Apr 2020 00:36:48: 19000000 INFO @ Thu, 16 Apr 2020 00:36:55: 27000000 INFO @ Thu, 16 Apr 2020 00:37:00: 22000000 INFO @ Thu, 16 Apr 2020 00:37:04: 20000000 INFO @ Thu, 16 Apr 2020 00:37:08: 28000000 INFO @ Thu, 16 Apr 2020 00:37:14: 23000000 INFO @ Thu, 16 Apr 2020 00:37:18: 21000000 INFO @ Thu, 16 Apr 2020 00:37:21: 29000000 INFO @ Thu, 16 Apr 2020 00:37:28: 24000000 INFO @ Thu, 16 Apr 2020 00:37:32: 22000000 INFO @ Thu, 16 Apr 2020 00:37:35: 30000000 INFO @ Thu, 16 Apr 2020 00:37:43: 25000000 INFO @ Thu, 16 Apr 2020 00:37:46: 23000000 INFO @ Thu, 16 Apr 2020 00:37:48: 31000000 INFO @ Thu, 16 Apr 2020 00:37:57: 26000000 INFO @ Thu, 16 Apr 2020 00:38:00: 24000000 INFO @ Thu, 16 Apr 2020 00:38:02: 32000000 INFO @ Thu, 16 Apr 2020 00:38:11: 27000000 INFO @ Thu, 16 Apr 2020 00:38:14: 25000000 INFO @ Thu, 16 Apr 2020 00:38:15: 33000000 INFO @ Thu, 16 Apr 2020 00:38:25: 28000000 INFO @ Thu, 16 Apr 2020 00:38:28: 34000000 INFO @ Thu, 16 Apr 2020 00:38:28: 26000000 INFO @ Thu, 16 Apr 2020 00:38:39: 29000000 INFO @ Thu, 16 Apr 2020 00:38:42: 35000000 INFO @ Thu, 16 Apr 2020 00:38:42: 27000000 INFO @ Thu, 16 Apr 2020 00:38:54: 30000000 INFO @ Thu, 16 Apr 2020 00:38:55: 36000000 INFO @ Thu, 16 Apr 2020 00:38:56: 28000000 INFO @ Thu, 16 Apr 2020 00:39:07: 31000000 INFO @ Thu, 16 Apr 2020 00:39:08: 37000000 INFO @ Thu, 16 Apr 2020 00:39:10: 29000000 INFO @ Thu, 16 Apr 2020 00:39:21: 38000000 INFO @ Thu, 16 Apr 2020 00:39:21: 32000000 INFO @ Thu, 16 Apr 2020 00:39:25: 30000000 INFO @ Thu, 16 Apr 2020 00:39:35: 39000000 INFO @ Thu, 16 Apr 2020 00:39:35: 33000000 INFO @ Thu, 16 Apr 2020 00:39:39: 31000000 INFO @ Thu, 16 Apr 2020 00:39:47: 40000000 INFO @ Thu, 16 Apr 2020 00:39:49: 34000000 INFO @ Thu, 16 Apr 2020 00:39:52: 32000000 INFO @ Thu, 16 Apr 2020 00:40:00: 41000000 INFO @ Thu, 16 Apr 2020 00:40:04: 35000000 INFO @ Thu, 16 Apr 2020 00:40:07: 33000000 INFO @ Thu, 16 Apr 2020 00:40:13: 42000000 INFO @ Thu, 16 Apr 2020 00:40:18: 36000000 INFO @ Thu, 16 Apr 2020 00:40:21: 34000000 INFO @ Thu, 16 Apr 2020 00:40:24: 43000000 INFO @ Thu, 16 Apr 2020 00:40:32: 37000000 INFO @ Thu, 16 Apr 2020 00:40:35: 35000000 INFO @ Thu, 16 Apr 2020 00:40:36: 44000000 INFO @ Thu, 16 Apr 2020 00:40:45: 38000000 INFO @ Thu, 16 Apr 2020 00:40:46: 45000000 INFO @ Thu, 16 Apr 2020 00:40:49: 36000000 INFO @ Thu, 16 Apr 2020 00:40:56: 46000000 INFO @ Thu, 16 Apr 2020 00:40:59: 39000000 INFO @ Thu, 16 Apr 2020 00:41:02: 37000000 INFO @ Thu, 16 Apr 2020 00:41:06: 47000000 INFO @ Thu, 16 Apr 2020 00:41:12: 40000000 INFO @ Thu, 16 Apr 2020 00:41:16: 48000000 INFO @ Thu, 16 Apr 2020 00:41:16: 38000000 INFO @ Thu, 16 Apr 2020 00:41:26: 41000000 INFO @ Thu, 16 Apr 2020 00:41:27: 49000000 INFO @ Thu, 16 Apr 2020 00:41:29: 39000000 INFO @ Thu, 16 Apr 2020 00:41:37: 50000000 INFO @ Thu, 16 Apr 2020 00:41:40: 42000000 INFO @ Thu, 16 Apr 2020 00:41:43: 40000000 INFO @ Thu, 16 Apr 2020 00:41:47: 51000000 INFO @ Thu, 16 Apr 2020 00:41:54: 43000000 INFO @ Thu, 16 Apr 2020 00:41:57: 52000000 INFO @ Thu, 16 Apr 2020 00:41:57: 41000000 INFO @ Thu, 16 Apr 2020 00:42:08: 53000000 INFO @ Thu, 16 Apr 2020 00:42:08: 44000000 INFO @ Thu, 16 Apr 2020 00:42:11: 42000000 INFO @ Thu, 16 Apr 2020 00:42:15: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:42:15: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:42:15: #1 total tags in treatment: 17433481 INFO @ Thu, 16 Apr 2020 00:42:15: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:42:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:42:15: #1 tags after filtering in treatment: 14375555 INFO @ Thu, 16 Apr 2020 00:42:15: #1 Redundant rate of treatment: 0.18 INFO @ Thu, 16 Apr 2020 00:42:15: #1 finished! INFO @ Thu, 16 Apr 2020 00:42:15: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:42:15: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:42:16: #2 number of paired peaks: 201 WARNING @ Thu, 16 Apr 2020 00:42:16: Fewer paired peaks (201) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 201 pairs to build model! INFO @ Thu, 16 Apr 2020 00:42:16: start model_add_line... INFO @ Thu, 16 Apr 2020 00:42:16: start X-correlation... INFO @ Thu, 16 Apr 2020 00:42:16: end of X-cor INFO @ Thu, 16 Apr 2020 00:42:16: #2 finished! INFO @ Thu, 16 Apr 2020 00:42:16: #2 predicted fragment length is 199 bps INFO @ Thu, 16 Apr 2020 00:42:16: #2 alternative fragment length(s) may be 176,199 bps INFO @ Thu, 16 Apr 2020 00:42:16: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619532/SRX6619532.05_model.r WARNING @ Thu, 16 Apr 2020 00:42:16: #2 Since the d (199) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:42:16: #2 You may need to consider one of the other alternative d(s): 176,199 WARNING @ Thu, 16 Apr 2020 00:42:16: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:42:16: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:42:16: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:42:22: 45000000 INFO @ Thu, 16 Apr 2020 00:42:25: 43000000 INFO @ Thu, 16 Apr 2020 00:42:36: 46000000 INFO @ Thu, 16 Apr 2020 00:42:39: 44000000 INFO @ Thu, 16 Apr 2020 00:42:43: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:42:49: 47000000 INFO @ Thu, 16 Apr 2020 00:42:52: 45000000 INFO @ Thu, 16 Apr 2020 00:42:57: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619532/SRX6619532.05_peaks.xls INFO @ Thu, 16 Apr 2020 00:42:57: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619532/SRX6619532.05_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:42:57: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619532/SRX6619532.05_summits.bed INFO @ Thu, 16 Apr 2020 00:42:57: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (444 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:43:02: 48000000 INFO @ Thu, 16 Apr 2020 00:43:05: 46000000 INFO @ Thu, 16 Apr 2020 00:43:16: 49000000 INFO @ Thu, 16 Apr 2020 00:43:19: 47000000 INFO @ Thu, 16 Apr 2020 00:43:29: 50000000 INFO @ Thu, 16 Apr 2020 00:43:32: 48000000 INFO @ Thu, 16 Apr 2020 00:43:42: 51000000 INFO @ Thu, 16 Apr 2020 00:43:45: 49000000 INFO @ Thu, 16 Apr 2020 00:43:55: 52000000 INFO @ Thu, 16 Apr 2020 00:43:58: 50000000 INFO @ Thu, 16 Apr 2020 00:44:08: 53000000 INFO @ Thu, 16 Apr 2020 00:44:11: 51000000 INFO @ Thu, 16 Apr 2020 00:44:16: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:44:16: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:44:16: #1 total tags in treatment: 17433481 INFO @ Thu, 16 Apr 2020 00:44:16: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:44:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:44:17: #1 tags after filtering in treatment: 14375555 INFO @ Thu, 16 Apr 2020 00:44:17: #1 Redundant rate of treatment: 0.18 INFO @ Thu, 16 Apr 2020 00:44:17: #1 finished! INFO @ Thu, 16 Apr 2020 00:44:17: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:44:17: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:44:18: #2 number of paired peaks: 201 WARNING @ Thu, 16 Apr 2020 00:44:18: Fewer paired peaks (201) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 201 pairs to build model! INFO @ Thu, 16 Apr 2020 00:44:18: start model_add_line... INFO @ Thu, 16 Apr 2020 00:44:18: start X-correlation... INFO @ Thu, 16 Apr 2020 00:44:18: end of X-cor INFO @ Thu, 16 Apr 2020 00:44:18: #2 finished! INFO @ Thu, 16 Apr 2020 00:44:18: #2 predicted fragment length is 199 bps INFO @ Thu, 16 Apr 2020 00:44:18: #2 alternative fragment length(s) may be 176,199 bps INFO @ Thu, 16 Apr 2020 00:44:18: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619532/SRX6619532.10_model.r WARNING @ Thu, 16 Apr 2020 00:44:18: #2 Since the d (199) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:44:18: #2 You may need to consider one of the other alternative d(s): 176,199 WARNING @ Thu, 16 Apr 2020 00:44:18: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:44:18: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:44:18: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:44:22: 52000000 INFO @ Thu, 16 Apr 2020 00:44:31: 53000000 INFO @ Thu, 16 Apr 2020 00:44:38: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:44:38: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:44:38: #1 total tags in treatment: 17433481 INFO @ Thu, 16 Apr 2020 00:44:38: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:44:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:44:38: #1 tags after filtering in treatment: 14375555 INFO @ Thu, 16 Apr 2020 00:44:38: #1 Redundant rate of treatment: 0.18 INFO @ Thu, 16 Apr 2020 00:44:38: #1 finished! INFO @ Thu, 16 Apr 2020 00:44:38: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:44:38: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:44:39: #2 number of paired peaks: 201 WARNING @ Thu, 16 Apr 2020 00:44:39: Fewer paired peaks (201) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 201 pairs to build model! INFO @ Thu, 16 Apr 2020 00:44:39: start model_add_line... INFO @ Thu, 16 Apr 2020 00:44:39: start X-correlation... INFO @ Thu, 16 Apr 2020 00:44:39: end of X-cor INFO @ Thu, 16 Apr 2020 00:44:39: #2 finished! INFO @ Thu, 16 Apr 2020 00:44:39: #2 predicted fragment length is 199 bps INFO @ Thu, 16 Apr 2020 00:44:39: #2 alternative fragment length(s) may be 176,199 bps INFO @ Thu, 16 Apr 2020 00:44:39: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619532/SRX6619532.20_model.r WARNING @ Thu, 16 Apr 2020 00:44:39: #2 Since the d (199) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:44:39: #2 You may need to consider one of the other alternative d(s): 176,199 WARNING @ Thu, 16 Apr 2020 00:44:39: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:44:39: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:44:39: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:44:44: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:44:59: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619532/SRX6619532.10_peaks.xls INFO @ Thu, 16 Apr 2020 00:44:59: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619532/SRX6619532.10_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:44:59: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619532/SRX6619532.10_summits.bed INFO @ Thu, 16 Apr 2020 00:44:59: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (315 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:45:06: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:45:20: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619532/SRX6619532.20_peaks.xls INFO @ Thu, 16 Apr 2020 00:45:20: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619532/SRX6619532.20_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:45:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619532/SRX6619532.20_summits.bed INFO @ Thu, 16 Apr 2020 00:45:20: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (209 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。