Job ID = 2590244 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 52,868,835 reads read : 52,868,835 reads written : 52,868,835 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:31 52868835 reads; of these: 52868835 (100.00%) were unpaired; of these: 5363206 (10.14%) aligned 0 times 42981399 (81.30%) aligned exactly 1 time 4524230 (8.56%) aligned >1 times 89.86% overall alignment rate Time searching: 00:16:31 Overall time: 00:16:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 14009421 / 47505629 = 0.2949 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 21:27:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5985676/SRX5985676.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5985676/SRX5985676.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5985676/SRX5985676.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5985676/SRX5985676.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 21:27:35: #1 read tag files... INFO @ Mon, 12 Aug 2019 21:27:35: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 21:27:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5985676/SRX5985676.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5985676/SRX5985676.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5985676/SRX5985676.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5985676/SRX5985676.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 21:27:36: #1 read tag files... INFO @ Mon, 12 Aug 2019 21:27:36: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 21:27:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5985676/SRX5985676.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5985676/SRX5985676.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5985676/SRX5985676.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5985676/SRX5985676.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 21:27:37: #1 read tag files... INFO @ Mon, 12 Aug 2019 21:27:37: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 21:27:45: 1000000 INFO @ Mon, 12 Aug 2019 21:27:46: 1000000 INFO @ Mon, 12 Aug 2019 21:27:46: 1000000 INFO @ Mon, 12 Aug 2019 21:27:54: 2000000 INFO @ Mon, 12 Aug 2019 21:27:54: 2000000 INFO @ Mon, 12 Aug 2019 21:27:55: 2000000 INFO @ Mon, 12 Aug 2019 21:28:01: 3000000 INFO @ Mon, 12 Aug 2019 21:28:03: 3000000 INFO @ Mon, 12 Aug 2019 21:28:04: 3000000 INFO @ Mon, 12 Aug 2019 21:28:09: 4000000 INFO @ Mon, 12 Aug 2019 21:28:12: 4000000 INFO @ Mon, 12 Aug 2019 21:28:13: 4000000 INFO @ Mon, 12 Aug 2019 21:28:17: 5000000 INFO @ Mon, 12 Aug 2019 21:28:21: 5000000 INFO @ Mon, 12 Aug 2019 21:28:21: 5000000 INFO @ Mon, 12 Aug 2019 21:28:24: 6000000 INFO @ Mon, 12 Aug 2019 21:28:30: 6000000 INFO @ Mon, 12 Aug 2019 21:28:30: 6000000 INFO @ Mon, 12 Aug 2019 21:28:32: 7000000 INFO @ Mon, 12 Aug 2019 21:28:39: 7000000 INFO @ Mon, 12 Aug 2019 21:28:40: 7000000 INFO @ Mon, 12 Aug 2019 21:28:40: 8000000 INFO @ Mon, 12 Aug 2019 21:28:48: 9000000 INFO @ Mon, 12 Aug 2019 21:28:49: 8000000 INFO @ Mon, 12 Aug 2019 21:28:49: 8000000 INFO @ Mon, 12 Aug 2019 21:28:56: 10000000 INFO @ Mon, 12 Aug 2019 21:28:58: 9000000 INFO @ Mon, 12 Aug 2019 21:28:59: 9000000 INFO @ Mon, 12 Aug 2019 21:29:04: 11000000 INFO @ Mon, 12 Aug 2019 21:29:07: 10000000 INFO @ Mon, 12 Aug 2019 21:29:08: 10000000 INFO @ Mon, 12 Aug 2019 21:29:11: 12000000 INFO @ Mon, 12 Aug 2019 21:29:17: 11000000 INFO @ Mon, 12 Aug 2019 21:29:17: 11000000 INFO @ Mon, 12 Aug 2019 21:29:19: 13000000 INFO @ Mon, 12 Aug 2019 21:29:26: 12000000 INFO @ Mon, 12 Aug 2019 21:29:26: 12000000 INFO @ Mon, 12 Aug 2019 21:29:27: 14000000 INFO @ Mon, 12 Aug 2019 21:29:35: 15000000 INFO @ Mon, 12 Aug 2019 21:29:36: 13000000 INFO @ Mon, 12 Aug 2019 21:29:36: 13000000 INFO @ Mon, 12 Aug 2019 21:29:42: 16000000 INFO @ Mon, 12 Aug 2019 21:29:45: 14000000 INFO @ Mon, 12 Aug 2019 21:29:45: 14000000 INFO @ Mon, 12 Aug 2019 21:29:50: 17000000 INFO @ Mon, 12 Aug 2019 21:29:55: 15000000 INFO @ Mon, 12 Aug 2019 21:29:55: 15000000 INFO @ Mon, 12 Aug 2019 21:29:58: 18000000 INFO @ Mon, 12 Aug 2019 21:30:04: 16000000 INFO @ Mon, 12 Aug 2019 21:30:04: 16000000 INFO @ Mon, 12 Aug 2019 21:30:06: 19000000 INFO @ Mon, 12 Aug 2019 21:30:13: 20000000 INFO @ Mon, 12 Aug 2019 21:30:13: 17000000 INFO @ Mon, 12 Aug 2019 21:30:14: 17000000 INFO @ Mon, 12 Aug 2019 21:30:21: 21000000 INFO @ Mon, 12 Aug 2019 21:30:23: 18000000 INFO @ Mon, 12 Aug 2019 21:30:23: 18000000 INFO @ Mon, 12 Aug 2019 21:30:29: 22000000 INFO @ Mon, 12 Aug 2019 21:30:32: 19000000 INFO @ Mon, 12 Aug 2019 21:30:32: 19000000 INFO @ Mon, 12 Aug 2019 21:30:37: 23000000 INFO @ Mon, 12 Aug 2019 21:30:41: 20000000 INFO @ Mon, 12 Aug 2019 21:30:42: 20000000 INFO @ Mon, 12 Aug 2019 21:30:44: 24000000 INFO @ Mon, 12 Aug 2019 21:30:51: 21000000 INFO @ Mon, 12 Aug 2019 21:30:51: 21000000 INFO @ Mon, 12 Aug 2019 21:30:52: 25000000 INFO @ Mon, 12 Aug 2019 21:31:00: 22000000 INFO @ Mon, 12 Aug 2019 21:31:00: 26000000 INFO @ Mon, 12 Aug 2019 21:31:00: 22000000 INFO @ Mon, 12 Aug 2019 21:31:07: 27000000 INFO @ Mon, 12 Aug 2019 21:31:09: 23000000 INFO @ Mon, 12 Aug 2019 21:31:10: 23000000 INFO @ Mon, 12 Aug 2019 21:31:15: 28000000 INFO @ Mon, 12 Aug 2019 21:31:18: 24000000 INFO @ Mon, 12 Aug 2019 21:31:19: 24000000 INFO @ Mon, 12 Aug 2019 21:31:23: 29000000 INFO @ Mon, 12 Aug 2019 21:31:28: 25000000 INFO @ Mon, 12 Aug 2019 21:31:28: 25000000 INFO @ Mon, 12 Aug 2019 21:31:31: 30000000 INFO @ Mon, 12 Aug 2019 21:31:38: 26000000 INFO @ Mon, 12 Aug 2019 21:31:38: 31000000 INFO @ Mon, 12 Aug 2019 21:31:39: 26000000 INFO @ Mon, 12 Aug 2019 21:31:46: 32000000 INFO @ Mon, 12 Aug 2019 21:31:47: 27000000 INFO @ Mon, 12 Aug 2019 21:31:49: 27000000 INFO @ Mon, 12 Aug 2019 21:31:54: 33000000 INFO @ Mon, 12 Aug 2019 21:31:56: 28000000 INFO @ Mon, 12 Aug 2019 21:31:58: #1 tag size is determined as 76 bps INFO @ Mon, 12 Aug 2019 21:31:58: #1 tag size = 76 INFO @ Mon, 12 Aug 2019 21:31:58: #1 total tags in treatment: 33496208 INFO @ Mon, 12 Aug 2019 21:31:58: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 21:31:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 21:31:59: #1 tags after filtering in treatment: 33496208 INFO @ Mon, 12 Aug 2019 21:31:59: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 21:31:59: #1 finished! INFO @ Mon, 12 Aug 2019 21:31:59: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 21:31:59: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 21:31:59: 28000000 INFO @ Mon, 12 Aug 2019 21:32:02: #2 number of paired peaks: 21 WARNING @ Mon, 12 Aug 2019 21:32:02: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 21:32:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX5985676/SRX5985676.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5985676/SRX5985676.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5985676/SRX5985676.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5985676/SRX5985676.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 21:32:04: 29000000 INFO @ Mon, 12 Aug 2019 21:32:09: 29000000 INFO @ Mon, 12 Aug 2019 21:32:13: 30000000 INFO @ Mon, 12 Aug 2019 21:32:19: 30000000 INFO @ Mon, 12 Aug 2019 21:32:22: 31000000 INFO @ Mon, 12 Aug 2019 21:32:28: 31000000 INFO @ Mon, 12 Aug 2019 21:32:31: 32000000 INFO @ Mon, 12 Aug 2019 21:32:38: 32000000 INFO @ Mon, 12 Aug 2019 21:32:40: 33000000 INFO @ Mon, 12 Aug 2019 21:32:44: #1 tag size is determined as 76 bps INFO @ Mon, 12 Aug 2019 21:32:44: #1 tag size = 76 INFO @ Mon, 12 Aug 2019 21:32:44: #1 total tags in treatment: 33496208 INFO @ Mon, 12 Aug 2019 21:32:44: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 21:32:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 21:32:45: #1 tags after filtering in treatment: 33496208 INFO @ Mon, 12 Aug 2019 21:32:45: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 21:32:45: #1 finished! INFO @ Mon, 12 Aug 2019 21:32:45: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 21:32:45: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 21:32:47: 33000000 INFO @ Mon, 12 Aug 2019 21:32:48: #2 number of paired peaks: 21 WARNING @ Mon, 12 Aug 2019 21:32:48: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 21:32:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX5985676/SRX5985676.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5985676/SRX5985676.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5985676/SRX5985676.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5985676/SRX5985676.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 21:32:52: #1 tag size is determined as 76 bps INFO @ Mon, 12 Aug 2019 21:32:52: #1 tag size = 76 INFO @ Mon, 12 Aug 2019 21:32:52: #1 total tags in treatment: 33496208 INFO @ Mon, 12 Aug 2019 21:32:52: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 21:32:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 21:32:53: #1 tags after filtering in treatment: 33496208 INFO @ Mon, 12 Aug 2019 21:32:53: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 21:32:53: #1 finished! INFO @ Mon, 12 Aug 2019 21:32:53: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 21:32:53: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 21:32:56: #2 number of paired peaks: 21 WARNING @ Mon, 12 Aug 2019 21:32:56: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 21:32:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX5985676/SRX5985676.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5985676/SRX5985676.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5985676/SRX5985676.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5985676/SRX5985676.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。