Job ID = 2590241 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 40,114,650 reads read : 40,114,650 reads written : 40,114,650 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:44 40114650 reads; of these: 40114650 (100.00%) were unpaired; of these: 330565 (0.82%) aligned 0 times 33389758 (83.24%) aligned exactly 1 time 6394327 (15.94%) aligned >1 times 99.18% overall alignment rate Time searching: 00:14:44 Overall time: 00:14:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 5536131 / 39784085 = 0.1392 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 21:03:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5985673/SRX5985673.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5985673/SRX5985673.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5985673/SRX5985673.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5985673/SRX5985673.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 21:03:10: #1 read tag files... INFO @ Mon, 12 Aug 2019 21:03:10: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 21:03:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5985673/SRX5985673.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5985673/SRX5985673.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5985673/SRX5985673.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5985673/SRX5985673.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 21:03:11: #1 read tag files... INFO @ Mon, 12 Aug 2019 21:03:11: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 21:03:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5985673/SRX5985673.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5985673/SRX5985673.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5985673/SRX5985673.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5985673/SRX5985673.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 21:03:12: #1 read tag files... INFO @ Mon, 12 Aug 2019 21:03:12: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 21:03:19: 1000000 INFO @ Mon, 12 Aug 2019 21:03:21: 1000000 INFO @ Mon, 12 Aug 2019 21:03:21: 1000000 INFO @ Mon, 12 Aug 2019 21:03:26: 2000000 INFO @ Mon, 12 Aug 2019 21:03:29: 2000000 INFO @ Mon, 12 Aug 2019 21:03:31: 2000000 INFO @ Mon, 12 Aug 2019 21:03:34: 3000000 INFO @ Mon, 12 Aug 2019 21:03:37: 3000000 INFO @ Mon, 12 Aug 2019 21:03:41: 3000000 INFO @ Mon, 12 Aug 2019 21:03:41: 4000000 INFO @ Mon, 12 Aug 2019 21:03:45: 4000000 INFO @ Mon, 12 Aug 2019 21:03:48: 5000000 INFO @ Mon, 12 Aug 2019 21:03:50: 4000000 INFO @ Mon, 12 Aug 2019 21:03:53: 5000000 INFO @ Mon, 12 Aug 2019 21:03:55: 6000000 INFO @ Mon, 12 Aug 2019 21:03:59: 5000000 INFO @ Mon, 12 Aug 2019 21:04:01: 6000000 INFO @ Mon, 12 Aug 2019 21:04:03: 7000000 INFO @ Mon, 12 Aug 2019 21:04:09: 6000000 INFO @ Mon, 12 Aug 2019 21:04:09: 7000000 INFO @ Mon, 12 Aug 2019 21:04:10: 8000000 INFO @ Mon, 12 Aug 2019 21:04:17: 9000000 INFO @ Mon, 12 Aug 2019 21:04:17: 8000000 INFO @ Mon, 12 Aug 2019 21:04:18: 7000000 INFO @ Mon, 12 Aug 2019 21:04:24: 10000000 INFO @ Mon, 12 Aug 2019 21:04:25: 9000000 INFO @ Mon, 12 Aug 2019 21:04:27: 8000000 INFO @ Mon, 12 Aug 2019 21:04:31: 11000000 INFO @ Mon, 12 Aug 2019 21:04:33: 10000000 INFO @ Mon, 12 Aug 2019 21:04:37: 9000000 INFO @ Mon, 12 Aug 2019 21:04:38: 12000000 INFO @ Mon, 12 Aug 2019 21:04:40: 11000000 INFO @ Mon, 12 Aug 2019 21:04:46: 13000000 INFO @ Mon, 12 Aug 2019 21:04:46: 10000000 INFO @ Mon, 12 Aug 2019 21:04:48: 12000000 INFO @ Mon, 12 Aug 2019 21:04:53: 14000000 INFO @ Mon, 12 Aug 2019 21:04:55: 11000000 INFO @ Mon, 12 Aug 2019 21:04:56: 13000000 INFO @ Mon, 12 Aug 2019 21:05:00: 15000000 INFO @ Mon, 12 Aug 2019 21:05:04: 12000000 INFO @ Mon, 12 Aug 2019 21:05:04: 14000000 INFO @ Mon, 12 Aug 2019 21:05:07: 16000000 INFO @ Mon, 12 Aug 2019 21:05:12: 15000000 INFO @ Mon, 12 Aug 2019 21:05:13: 13000000 INFO @ Mon, 12 Aug 2019 21:05:14: 17000000 INFO @ Mon, 12 Aug 2019 21:05:20: 16000000 INFO @ Mon, 12 Aug 2019 21:05:21: 18000000 INFO @ Mon, 12 Aug 2019 21:05:23: 14000000 INFO @ Mon, 12 Aug 2019 21:05:28: 17000000 INFO @ Mon, 12 Aug 2019 21:05:29: 19000000 INFO @ Mon, 12 Aug 2019 21:05:32: 15000000 INFO @ Mon, 12 Aug 2019 21:05:36: 18000000 INFO @ Mon, 12 Aug 2019 21:05:36: 20000000 INFO @ Mon, 12 Aug 2019 21:05:41: 16000000 INFO @ Mon, 12 Aug 2019 21:05:43: 21000000 INFO @ Mon, 12 Aug 2019 21:05:44: 19000000 INFO @ Mon, 12 Aug 2019 21:05:50: 22000000 INFO @ Mon, 12 Aug 2019 21:05:50: 17000000 INFO @ Mon, 12 Aug 2019 21:05:51: 20000000 INFO @ Mon, 12 Aug 2019 21:05:57: 23000000 INFO @ Mon, 12 Aug 2019 21:05:59: 21000000 INFO @ Mon, 12 Aug 2019 21:06:00: 18000000 INFO @ Mon, 12 Aug 2019 21:06:06: 24000000 INFO @ Mon, 12 Aug 2019 21:06:07: 22000000 INFO @ Mon, 12 Aug 2019 21:06:09: 19000000 INFO @ Mon, 12 Aug 2019 21:06:13: 25000000 INFO @ Mon, 12 Aug 2019 21:06:15: 23000000 INFO @ Mon, 12 Aug 2019 21:06:18: 20000000 INFO @ Mon, 12 Aug 2019 21:06:20: 26000000 INFO @ Mon, 12 Aug 2019 21:06:24: 24000000 INFO @ Mon, 12 Aug 2019 21:06:27: 21000000 INFO @ Mon, 12 Aug 2019 21:06:27: 27000000 INFO @ Mon, 12 Aug 2019 21:06:32: 25000000 INFO @ Mon, 12 Aug 2019 21:06:35: 28000000 INFO @ Mon, 12 Aug 2019 21:06:37: 22000000 INFO @ Mon, 12 Aug 2019 21:06:40: 26000000 INFO @ Mon, 12 Aug 2019 21:06:42: 29000000 INFO @ Mon, 12 Aug 2019 21:06:46: 23000000 INFO @ Mon, 12 Aug 2019 21:06:48: 27000000 INFO @ Mon, 12 Aug 2019 21:06:49: 30000000 INFO @ Mon, 12 Aug 2019 21:06:55: 24000000 INFO @ Mon, 12 Aug 2019 21:06:56: 28000000 INFO @ Mon, 12 Aug 2019 21:06:56: 31000000 INFO @ Mon, 12 Aug 2019 21:07:03: 32000000 INFO @ Mon, 12 Aug 2019 21:07:04: 29000000 INFO @ Mon, 12 Aug 2019 21:07:04: 25000000 INFO @ Mon, 12 Aug 2019 21:07:10: 33000000 INFO @ Mon, 12 Aug 2019 21:07:11: 30000000 INFO @ Mon, 12 Aug 2019 21:07:13: 26000000 INFO @ Mon, 12 Aug 2019 21:07:18: 34000000 INFO @ Mon, 12 Aug 2019 21:07:19: 31000000 INFO @ Mon, 12 Aug 2019 21:07:20: #1 tag size is determined as 76 bps INFO @ Mon, 12 Aug 2019 21:07:20: #1 tag size = 76 INFO @ Mon, 12 Aug 2019 21:07:20: #1 total tags in treatment: 34247954 INFO @ Mon, 12 Aug 2019 21:07:20: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 21:07:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 21:07:20: #1 tags after filtering in treatment: 34247954 INFO @ Mon, 12 Aug 2019 21:07:20: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 21:07:20: #1 finished! INFO @ Mon, 12 Aug 2019 21:07:20: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 21:07:20: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 21:07:22: 27000000 INFO @ Mon, 12 Aug 2019 21:07:23: #2 number of paired peaks: 40 WARNING @ Mon, 12 Aug 2019 21:07:23: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 21:07:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX5985673/SRX5985673.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5985673/SRX5985673.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5985673/SRX5985673.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5985673/SRX5985673.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 21:07:27: 32000000 INFO @ Mon, 12 Aug 2019 21:07:32: 28000000 INFO @ Mon, 12 Aug 2019 21:07:35: 33000000 INFO @ Mon, 12 Aug 2019 21:07:41: 29000000 INFO @ Mon, 12 Aug 2019 21:07:43: 34000000 INFO @ Mon, 12 Aug 2019 21:07:45: #1 tag size is determined as 76 bps INFO @ Mon, 12 Aug 2019 21:07:45: #1 tag size = 76 INFO @ Mon, 12 Aug 2019 21:07:45: #1 total tags in treatment: 34247954 INFO @ Mon, 12 Aug 2019 21:07:45: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 21:07:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 21:07:46: #1 tags after filtering in treatment: 34247954 INFO @ Mon, 12 Aug 2019 21:07:46: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 21:07:46: #1 finished! INFO @ Mon, 12 Aug 2019 21:07:46: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 21:07:46: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 21:07:48: #2 number of paired peaks: 40 WARNING @ Mon, 12 Aug 2019 21:07:48: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 21:07:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX5985673/SRX5985673.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5985673/SRX5985673.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5985673/SRX5985673.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5985673/SRX5985673.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 21:07:50: 30000000 INFO @ Mon, 12 Aug 2019 21:07:59: 31000000 INFO @ Mon, 12 Aug 2019 21:08:08: 32000000 INFO @ Mon, 12 Aug 2019 21:08:17: 33000000 INFO @ Mon, 12 Aug 2019 21:08:26: 34000000 INFO @ Mon, 12 Aug 2019 21:08:29: #1 tag size is determined as 76 bps INFO @ Mon, 12 Aug 2019 21:08:29: #1 tag size = 76 INFO @ Mon, 12 Aug 2019 21:08:29: #1 total tags in treatment: 34247954 INFO @ Mon, 12 Aug 2019 21:08:29: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 21:08:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 21:08:30: #1 tags after filtering in treatment: 34247954 INFO @ Mon, 12 Aug 2019 21:08:30: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 21:08:30: #1 finished! INFO @ Mon, 12 Aug 2019 21:08:30: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 21:08:30: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 21:08:32: #2 number of paired peaks: 40 WARNING @ Mon, 12 Aug 2019 21:08:32: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 21:08:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX5985673/SRX5985673.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5985673/SRX5985673.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5985673/SRX5985673.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5985673/SRX5985673.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。