Job ID = 5720152 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 19,594,831 reads read : 39,189,662 reads written : 19,594,831 reads 0-length : 19,594,831 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:51 19594831 reads; of these: 19594831 (100.00%) were unpaired; of these: 2952220 (15.07%) aligned 0 times 13725205 (70.05%) aligned exactly 1 time 2917406 (14.89%) aligned >1 times 84.93% overall alignment rate Time searching: 00:03:51 Overall time: 00:03:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 12842203 / 16642611 = 0.7716 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 15 Apr 2020 21:23:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5702595/SRX5702595.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5702595/SRX5702595.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5702595/SRX5702595.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5702595/SRX5702595.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 15 Apr 2020 21:23:18: #1 read tag files... INFO @ Wed, 15 Apr 2020 21:23:18: #1 read treatment tags... INFO @ Wed, 15 Apr 2020 21:23:23: 1000000 INFO @ Wed, 15 Apr 2020 21:23:28: 2000000 INFO @ Wed, 15 Apr 2020 21:23:33: 3000000 INFO @ Wed, 15 Apr 2020 21:23:37: #1 tag size is determined as 50 bps INFO @ Wed, 15 Apr 2020 21:23:37: #1 tag size = 50 INFO @ Wed, 15 Apr 2020 21:23:37: #1 total tags in treatment: 3800408 INFO @ Wed, 15 Apr 2020 21:23:37: #1 user defined the maximum tags... INFO @ Wed, 15 Apr 2020 21:23:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 15 Apr 2020 21:23:37: #1 tags after filtering in treatment: 3800408 INFO @ Wed, 15 Apr 2020 21:23:37: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 15 Apr 2020 21:23:37: #1 finished! INFO @ Wed, 15 Apr 2020 21:23:37: #2 Build Peak Model... INFO @ Wed, 15 Apr 2020 21:23:37: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 15 Apr 2020 21:23:38: #2 number of paired peaks: 659 WARNING @ Wed, 15 Apr 2020 21:23:38: Fewer paired peaks (659) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 659 pairs to build model! INFO @ Wed, 15 Apr 2020 21:23:38: start model_add_line... INFO @ Wed, 15 Apr 2020 21:23:38: start X-correlation... INFO @ Wed, 15 Apr 2020 21:23:38: end of X-cor INFO @ Wed, 15 Apr 2020 21:23:38: #2 finished! INFO @ Wed, 15 Apr 2020 21:23:38: #2 predicted fragment length is 50 bps INFO @ Wed, 15 Apr 2020 21:23:38: #2 alternative fragment length(s) may be 4,50,544 bps INFO @ Wed, 15 Apr 2020 21:23:38: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX5702595/SRX5702595.05_model.r WARNING @ Wed, 15 Apr 2020 21:23:38: #2 Since the d (50) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 15 Apr 2020 21:23:38: #2 You may need to consider one of the other alternative d(s): 4,50,544 WARNING @ Wed, 15 Apr 2020 21:23:38: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 15 Apr 2020 21:23:38: #3 Call peaks... INFO @ Wed, 15 Apr 2020 21:23:38: #3 Pre-compute pvalue-qvalue table... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 15 Apr 2020 21:23:46: #3 Call peaks for each chromosome... INFO @ Wed, 15 Apr 2020 21:23:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5702595/SRX5702595.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5702595/SRX5702595.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5702595/SRX5702595.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5702595/SRX5702595.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 15 Apr 2020 21:23:46: #1 read tag files... INFO @ Wed, 15 Apr 2020 21:23:46: #1 read treatment tags... INFO @ Wed, 15 Apr 2020 21:23:50: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX5702595/SRX5702595.05_peaks.xls INFO @ Wed, 15 Apr 2020 21:23:50: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX5702595/SRX5702595.05_peaks.narrowPeak INFO @ Wed, 15 Apr 2020 21:23:50: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX5702595/SRX5702595.05_summits.bed INFO @ Wed, 15 Apr 2020 21:23:50: Done! INFO @ Wed, 15 Apr 2020 21:23:52: 1000000 pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (746 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Wed, 15 Apr 2020 21:23:57: 2000000 INFO @ Wed, 15 Apr 2020 21:24:02: 3000000 INFO @ Wed, 15 Apr 2020 21:24:06: #1 tag size is determined as 50 bps INFO @ Wed, 15 Apr 2020 21:24:06: #1 tag size = 50 INFO @ Wed, 15 Apr 2020 21:24:06: #1 total tags in treatment: 3800408 INFO @ Wed, 15 Apr 2020 21:24:06: #1 user defined the maximum tags... INFO @ Wed, 15 Apr 2020 21:24:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 15 Apr 2020 21:24:06: #1 tags after filtering in treatment: 3800408 INFO @ Wed, 15 Apr 2020 21:24:06: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 15 Apr 2020 21:24:06: #1 finished! INFO @ Wed, 15 Apr 2020 21:24:06: #2 Build Peak Model... INFO @ Wed, 15 Apr 2020 21:24:06: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 15 Apr 2020 21:24:07: #2 number of paired peaks: 659 WARNING @ Wed, 15 Apr 2020 21:24:07: Fewer paired peaks (659) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 659 pairs to build model! INFO @ Wed, 15 Apr 2020 21:24:07: start model_add_line... INFO @ Wed, 15 Apr 2020 21:24:07: start X-correlation... INFO @ Wed, 15 Apr 2020 21:24:07: end of X-cor INFO @ Wed, 15 Apr 2020 21:24:07: #2 finished! INFO @ Wed, 15 Apr 2020 21:24:07: #2 predicted fragment length is 50 bps INFO @ Wed, 15 Apr 2020 21:24:07: #2 alternative fragment length(s) may be 4,50,544 bps INFO @ Wed, 15 Apr 2020 21:24:07: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX5702595/SRX5702595.10_model.r WARNING @ Wed, 15 Apr 2020 21:24:07: #2 Since the d (50) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 15 Apr 2020 21:24:07: #2 You may need to consider one of the other alternative d(s): 4,50,544 WARNING @ Wed, 15 Apr 2020 21:24:07: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 15 Apr 2020 21:24:07: #3 Call peaks... INFO @ Wed, 15 Apr 2020 21:24:07: #3 Pre-compute pvalue-qvalue table... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 15 Apr 2020 21:24:15: #3 Call peaks for each chromosome... INFO @ Wed, 15 Apr 2020 21:24:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5702595/SRX5702595.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5702595/SRX5702595.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5702595/SRX5702595.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5702595/SRX5702595.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 15 Apr 2020 21:24:16: #1 read tag files... INFO @ Wed, 15 Apr 2020 21:24:16: #1 read treatment tags... INFO @ Wed, 15 Apr 2020 21:24:19: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX5702595/SRX5702595.10_peaks.xls INFO @ Wed, 15 Apr 2020 21:24:19: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX5702595/SRX5702595.10_peaks.narrowPeak INFO @ Wed, 15 Apr 2020 21:24:19: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX5702595/SRX5702595.10_summits.bed INFO @ Wed, 15 Apr 2020 21:24:19: Done! INFO @ Wed, 15 Apr 2020 21:24:21: 1000000 pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (482 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Wed, 15 Apr 2020 21:24:27: 2000000 INFO @ Wed, 15 Apr 2020 21:24:32: 3000000 INFO @ Wed, 15 Apr 2020 21:24:36: #1 tag size is determined as 50 bps INFO @ Wed, 15 Apr 2020 21:24:36: #1 tag size = 50 INFO @ Wed, 15 Apr 2020 21:24:36: #1 total tags in treatment: 3800408 INFO @ Wed, 15 Apr 2020 21:24:36: #1 user defined the maximum tags... INFO @ Wed, 15 Apr 2020 21:24:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 15 Apr 2020 21:24:36: #1 tags after filtering in treatment: 3800408 INFO @ Wed, 15 Apr 2020 21:24:36: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 15 Apr 2020 21:24:36: #1 finished! INFO @ Wed, 15 Apr 2020 21:24:36: #2 Build Peak Model... INFO @ Wed, 15 Apr 2020 21:24:36: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 15 Apr 2020 21:24:36: #2 number of paired peaks: 659 WARNING @ Wed, 15 Apr 2020 21:24:36: Fewer paired peaks (659) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 659 pairs to build model! INFO @ Wed, 15 Apr 2020 21:24:36: start model_add_line... INFO @ Wed, 15 Apr 2020 21:24:36: start X-correlation... INFO @ Wed, 15 Apr 2020 21:24:36: end of X-cor INFO @ Wed, 15 Apr 2020 21:24:36: #2 finished! INFO @ Wed, 15 Apr 2020 21:24:36: #2 predicted fragment length is 50 bps INFO @ Wed, 15 Apr 2020 21:24:36: #2 alternative fragment length(s) may be 4,50,544 bps INFO @ Wed, 15 Apr 2020 21:24:36: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX5702595/SRX5702595.20_model.r WARNING @ Wed, 15 Apr 2020 21:24:36: #2 Since the d (50) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 15 Apr 2020 21:24:36: #2 You may need to consider one of the other alternative d(s): 4,50,544 WARNING @ Wed, 15 Apr 2020 21:24:36: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 15 Apr 2020 21:24:36: #3 Call peaks... INFO @ Wed, 15 Apr 2020 21:24:36: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 15 Apr 2020 21:24:44: #3 Call peaks for each chromosome... INFO @ Wed, 15 Apr 2020 21:24:48: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX5702595/SRX5702595.20_peaks.xls INFO @ Wed, 15 Apr 2020 21:24:48: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX5702595/SRX5702595.20_peaks.narrowPeak INFO @ Wed, 15 Apr 2020 21:24:48: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX5702595/SRX5702595.20_summits.bed INFO @ Wed, 15 Apr 2020 21:24:48: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (206 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。