Job ID = 3785783 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-11-01T05:28:16 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:28:16 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:31:28 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:31:28 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:31:28 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:31:28 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:33:01 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:33:01 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:33:01 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:33:01 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:34:05 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:34:05 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:34:05 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:35:38 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:36:13 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:36:13 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '130.14.250.26' from '172.19.7.18' 2019-11-01T05:36:13 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (130.14.250.26) from '172.19.7.18' 2019-11-01T05:36:28 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:36:28 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:36:28 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:36:40 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:36:40 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:37:48 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:37:56 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:38:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:39:46 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:39:51 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:40:06 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:40:42 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:40:42 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:41:16 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:41:16 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '130.14.250.25' from '172.19.7.18' 2019-11-01T05:41:16 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (130.14.250.25) from '172.19.7.18' 2019-11-01T05:41:17 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:41:27 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:41:53 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:42:20 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:43:28 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:44:06 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:44:44 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:44:54 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:45:33 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:46:13 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:46:16 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:46:23 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:46:33 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:47:13 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:47:58 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:48:25 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:49:32 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:49:57 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:50:18 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:50:19 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:51:52 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:52:33 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:52:33 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:52:50 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:53:07 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:53:07 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '130.14.250.24' from '172.19.7.18' 2019-11-01T05:53:07 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (130.14.250.24) from '172.19.7.18' 2019-11-01T05:53:15 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:53:42 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:54:51 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:55:22 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:55:25 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:56:00 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:56:22 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:57:20 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:57:44 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:57:44 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:57:47 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:58:12 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:58:54 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:59:54 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 83,662,833 reads read : 167,325,666 reads written : 83,662,833 reads 0-length : 83,662,833 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:19:46 83662833 reads; of these: 83662833 (100.00%) were unpaired; of these: 5964555 (7.13%) aligned 0 times 65205119 (77.94%) aligned exactly 1 time 12493159 (14.93%) aligned >1 times 92.87% overall alignment rate Time searching: 00:19:46 Overall time: 00:19:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 28007799 / 77698278 = 0.3605 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Fri, 01 Nov 2019 15:53:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5545245/SRX5545245.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5545245/SRX5545245.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5545245/SRX5545245.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5545245/SRX5545245.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 01 Nov 2019 15:53:16: #1 read tag files... INFO @ Fri, 01 Nov 2019 15:53:16: #1 read treatment tags... INFO @ Fri, 01 Nov 2019 15:53:22: 1000000 INFO @ Fri, 01 Nov 2019 15:53:29: 2000000 INFO @ Fri, 01 Nov 2019 15:53:36: 3000000 INFO @ Fri, 01 Nov 2019 15:53:43: 4000000 INFO @ Fri, 01 Nov 2019 15:53:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5545245/SRX5545245.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5545245/SRX5545245.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5545245/SRX5545245.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5545245/SRX5545245.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 01 Nov 2019 15:53:45: #1 read tag files... INFO @ Fri, 01 Nov 2019 15:53:45: #1 read treatment tags... INFO @ Fri, 01 Nov 2019 15:53:49: 5000000 INFO @ Fri, 01 Nov 2019 15:53:52: 1000000 INFO @ Fri, 01 Nov 2019 15:53:56: 6000000 INFO @ Fri, 01 Nov 2019 15:53:58: 2000000 INFO @ Fri, 01 Nov 2019 15:54:02: 7000000 INFO @ Fri, 01 Nov 2019 15:54:04: 3000000 INFO @ Fri, 01 Nov 2019 15:54:09: 8000000 INFO @ Fri, 01 Nov 2019 15:54:10: 4000000 BedGraph に変換中... INFO @ Fri, 01 Nov 2019 15:54:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5545245/SRX5545245.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5545245/SRX5545245.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5545245/SRX5545245.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5545245/SRX5545245.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 01 Nov 2019 15:54:15: #1 read tag files... INFO @ Fri, 01 Nov 2019 15:54:15: #1 read treatment tags... INFO @ Fri, 01 Nov 2019 15:54:15: 9000000 INFO @ Fri, 01 Nov 2019 15:54:16: 5000000 INFO @ Fri, 01 Nov 2019 15:54:22: 1000000 INFO @ Fri, 01 Nov 2019 15:54:22: 10000000 INFO @ Fri, 01 Nov 2019 15:54:22: 6000000 INFO @ Fri, 01 Nov 2019 15:54:28: 2000000 INFO @ Fri, 01 Nov 2019 15:54:29: 7000000 INFO @ Fri, 01 Nov 2019 15:54:29: 11000000 INFO @ Fri, 01 Nov 2019 15:54:34: 3000000 INFO @ Fri, 01 Nov 2019 15:54:35: 8000000 INFO @ Fri, 01 Nov 2019 15:54:35: 12000000 INFO @ Fri, 01 Nov 2019 15:54:40: 4000000 INFO @ Fri, 01 Nov 2019 15:54:41: 9000000 INFO @ Fri, 01 Nov 2019 15:54:42: 13000000 INFO @ Fri, 01 Nov 2019 15:54:46: 5000000 INFO @ Fri, 01 Nov 2019 15:54:47: 10000000 INFO @ Fri, 01 Nov 2019 15:54:48: 14000000 INFO @ Fri, 01 Nov 2019 15:54:52: 6000000 INFO @ Fri, 01 Nov 2019 15:54:53: 11000000 INFO @ Fri, 01 Nov 2019 15:54:55: 15000000 INFO @ Fri, 01 Nov 2019 15:54:59: 7000000 INFO @ Fri, 01 Nov 2019 15:54:59: 12000000 INFO @ Fri, 01 Nov 2019 15:55:02: 16000000 INFO @ Fri, 01 Nov 2019 15:55:05: 8000000 INFO @ Fri, 01 Nov 2019 15:55:05: 13000000 INFO @ Fri, 01 Nov 2019 15:55:08: 17000000 INFO @ Fri, 01 Nov 2019 15:55:11: 9000000 INFO @ Fri, 01 Nov 2019 15:55:11: 14000000 INFO @ Fri, 01 Nov 2019 15:55:15: 18000000 INFO @ Fri, 01 Nov 2019 15:55:17: 10000000 INFO @ Fri, 01 Nov 2019 15:55:18: 15000000 INFO @ Fri, 01 Nov 2019 15:55:21: 19000000 INFO @ Fri, 01 Nov 2019 15:55:23: 11000000 INFO @ Fri, 01 Nov 2019 15:55:24: 16000000 INFO @ Fri, 01 Nov 2019 15:55:28: 20000000 INFO @ Fri, 01 Nov 2019 15:55:29: 12000000 INFO @ Fri, 01 Nov 2019 15:55:30: 17000000 INFO @ Fri, 01 Nov 2019 15:55:34: 21000000 INFO @ Fri, 01 Nov 2019 15:55:35: 13000000 INFO @ Fri, 01 Nov 2019 15:55:36: 18000000 INFO @ Fri, 01 Nov 2019 15:55:41: 22000000 INFO @ Fri, 01 Nov 2019 15:55:41: 14000000 INFO @ Fri, 01 Nov 2019 15:55:42: 19000000 INFO @ Fri, 01 Nov 2019 15:55:47: 23000000 INFO @ Fri, 01 Nov 2019 15:55:48: 15000000 INFO @ Fri, 01 Nov 2019 15:55:48: 20000000 INFO @ Fri, 01 Nov 2019 15:55:54: 16000000 INFO @ Fri, 01 Nov 2019 15:55:54: 24000000 INFO @ Fri, 01 Nov 2019 15:55:54: 21000000 INFO @ Fri, 01 Nov 2019 15:56:00: 17000000 INFO @ Fri, 01 Nov 2019 15:56:00: 25000000 INFO @ Fri, 01 Nov 2019 15:56:00: 22000000 INFO @ Fri, 01 Nov 2019 15:56:06: 18000000 INFO @ Fri, 01 Nov 2019 15:56:07: 23000000 INFO @ Fri, 01 Nov 2019 15:56:07: 26000000 INFO @ Fri, 01 Nov 2019 15:56:12: 19000000 INFO @ Fri, 01 Nov 2019 15:56:13: 24000000 INFO @ Fri, 01 Nov 2019 15:56:13: 27000000 INFO @ Fri, 01 Nov 2019 15:56:18: 20000000 INFO @ Fri, 01 Nov 2019 15:56:19: 25000000 INFO @ Fri, 01 Nov 2019 15:56:20: 28000000 INFO @ Fri, 01 Nov 2019 15:56:24: 21000000 INFO @ Fri, 01 Nov 2019 15:56:25: 26000000 INFO @ Fri, 01 Nov 2019 15:56:26: 29000000 INFO @ Fri, 01 Nov 2019 15:56:30: 22000000 INFO @ Fri, 01 Nov 2019 15:56:31: 27000000 INFO @ Fri, 01 Nov 2019 15:56:33: 30000000 INFO @ Fri, 01 Nov 2019 15:56:36: 23000000 INFO @ Fri, 01 Nov 2019 15:56:37: 28000000 INFO @ Fri, 01 Nov 2019 15:56:39: 31000000 INFO @ Fri, 01 Nov 2019 15:56:43: 24000000 INFO @ Fri, 01 Nov 2019 15:56:43: 29000000 INFO @ Fri, 01 Nov 2019 15:56:46: 32000000 INFO @ Fri, 01 Nov 2019 15:56:49: 25000000 INFO @ Fri, 01 Nov 2019 15:56:49: 30000000 INFO @ Fri, 01 Nov 2019 15:56:52: 33000000 INFO @ Fri, 01 Nov 2019 15:56:55: 26000000 INFO @ Fri, 01 Nov 2019 15:56:55: 31000000 INFO @ Fri, 01 Nov 2019 15:56:59: 34000000 INFO @ Fri, 01 Nov 2019 15:57:01: 27000000 INFO @ Fri, 01 Nov 2019 15:57:01: 32000000 INFO @ Fri, 01 Nov 2019 15:57:05: 35000000 INFO @ Fri, 01 Nov 2019 15:57:07: 28000000 INFO @ Fri, 01 Nov 2019 15:57:07: 33000000 INFO @ Fri, 01 Nov 2019 15:57:12: 36000000 INFO @ Fri, 01 Nov 2019 15:57:13: 29000000 INFO @ Fri, 01 Nov 2019 15:57:13: 34000000 INFO @ Fri, 01 Nov 2019 15:57:18: 37000000 INFO @ Fri, 01 Nov 2019 15:57:19: 30000000 INFO @ Fri, 01 Nov 2019 15:57:19: 35000000 INFO @ Fri, 01 Nov 2019 15:57:25: 38000000 INFO @ Fri, 01 Nov 2019 15:57:25: 31000000 INFO @ Fri, 01 Nov 2019 15:57:26: 36000000 INFO @ Fri, 01 Nov 2019 15:57:31: 32000000 INFO @ Fri, 01 Nov 2019 15:57:31: 39000000 INFO @ Fri, 01 Nov 2019 15:57:32: 37000000 INFO @ Fri, 01 Nov 2019 15:57:37: 33000000 INFO @ Fri, 01 Nov 2019 15:57:38: 38000000 INFO @ Fri, 01 Nov 2019 15:57:38: 40000000 INFO @ Fri, 01 Nov 2019 15:57:43: 34000000 INFO @ Fri, 01 Nov 2019 15:57:44: 39000000 INFO @ Fri, 01 Nov 2019 15:57:44: 41000000 INFO @ Fri, 01 Nov 2019 15:57:49: 35000000 INFO @ Fri, 01 Nov 2019 15:57:50: 40000000 INFO @ Fri, 01 Nov 2019 15:57:51: 42000000 INFO @ Fri, 01 Nov 2019 15:57:55: 36000000 INFO @ Fri, 01 Nov 2019 15:57:56: 41000000 INFO @ Fri, 01 Nov 2019 15:57:57: 43000000 INFO @ Fri, 01 Nov 2019 15:58:02: 37000000 INFO @ Fri, 01 Nov 2019 15:58:02: 42000000 INFO @ Fri, 01 Nov 2019 15:58:04: 44000000 INFO @ Fri, 01 Nov 2019 15:58:08: 38000000 INFO @ Fri, 01 Nov 2019 15:58:08: 43000000 INFO @ Fri, 01 Nov 2019 15:58:11: 45000000 INFO @ Fri, 01 Nov 2019 15:58:14: 39000000 INFO @ Fri, 01 Nov 2019 15:58:14: 44000000 INFO @ Fri, 01 Nov 2019 15:58:18: 46000000 INFO @ Fri, 01 Nov 2019 15:58:20: 40000000 INFO @ Fri, 01 Nov 2019 15:58:21: 45000000 INFO @ Fri, 01 Nov 2019 15:58:24: 47000000 INFO @ Fri, 01 Nov 2019 15:58:26: 41000000 INFO @ Fri, 01 Nov 2019 15:58:27: 46000000 INFO @ Fri, 01 Nov 2019 15:58:31: 48000000 INFO @ Fri, 01 Nov 2019 15:58:32: 42000000 INFO @ Fri, 01 Nov 2019 15:58:33: 47000000 INFO @ Fri, 01 Nov 2019 15:58:37: 49000000 INFO @ Fri, 01 Nov 2019 15:58:38: 43000000 INFO @ Fri, 01 Nov 2019 15:58:39: 48000000 INFO @ Fri, 01 Nov 2019 15:58:43: #1 tag size is determined as 51 bps INFO @ Fri, 01 Nov 2019 15:58:43: #1 tag size = 51 INFO @ Fri, 01 Nov 2019 15:58:43: #1 total tags in treatment: 49690479 INFO @ Fri, 01 Nov 2019 15:58:43: #1 user defined the maximum tags... INFO @ Fri, 01 Nov 2019 15:58:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 01 Nov 2019 15:58:44: #1 tags after filtering in treatment: 49690479 INFO @ Fri, 01 Nov 2019 15:58:44: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 01 Nov 2019 15:58:44: #1 finished! INFO @ Fri, 01 Nov 2019 15:58:44: #2 Build Peak Model... INFO @ Fri, 01 Nov 2019 15:58:44: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 01 Nov 2019 15:58:44: 44000000 INFO @ Fri, 01 Nov 2019 15:58:45: 49000000 INFO @ Fri, 01 Nov 2019 15:58:48: #2 number of paired peaks: 0 WARNING @ Fri, 01 Nov 2019 15:58:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 01 Nov 2019 15:58:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX5545245/SRX5545245.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5545245/SRX5545245.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5545245/SRX5545245.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5545245/SRX5545245.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 01 Nov 2019 15:58:50: #1 tag size is determined as 51 bps INFO @ Fri, 01 Nov 2019 15:58:50: #1 tag size = 51 INFO @ Fri, 01 Nov 2019 15:58:50: #1 total tags in treatment: 49690479 INFO @ Fri, 01 Nov 2019 15:58:50: #1 user defined the maximum tags... INFO @ Fri, 01 Nov 2019 15:58:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 01 Nov 2019 15:58:51: 45000000 INFO @ Fri, 01 Nov 2019 15:58:51: #1 tags after filtering in treatment: 49690479 INFO @ Fri, 01 Nov 2019 15:58:51: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 01 Nov 2019 15:58:51: #1 finished! INFO @ Fri, 01 Nov 2019 15:58:51: #2 Build Peak Model... INFO @ Fri, 01 Nov 2019 15:58:51: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 01 Nov 2019 15:58:55: #2 number of paired peaks: 0 WARNING @ Fri, 01 Nov 2019 15:58:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 01 Nov 2019 15:58:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX5545245/SRX5545245.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5545245/SRX5545245.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5545245/SRX5545245.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5545245/SRX5545245.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 01 Nov 2019 15:58:57: 46000000 INFO @ Fri, 01 Nov 2019 15:59:03: 47000000 INFO @ Fri, 01 Nov 2019 15:59:09: 48000000 INFO @ Fri, 01 Nov 2019 15:59:15: 49000000 INFO @ Fri, 01 Nov 2019 15:59:20: #1 tag size is determined as 51 bps INFO @ Fri, 01 Nov 2019 15:59:20: #1 tag size = 51 INFO @ Fri, 01 Nov 2019 15:59:20: #1 total tags in treatment: 49690479 INFO @ Fri, 01 Nov 2019 15:59:20: #1 user defined the maximum tags... INFO @ Fri, 01 Nov 2019 15:59:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 01 Nov 2019 15:59:21: #1 tags after filtering in treatment: 49690479 INFO @ Fri, 01 Nov 2019 15:59:21: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 01 Nov 2019 15:59:21: #1 finished! INFO @ Fri, 01 Nov 2019 15:59:21: #2 Build Peak Model... INFO @ Fri, 01 Nov 2019 15:59:21: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 01 Nov 2019 15:59:25: #2 number of paired peaks: 0 WARNING @ Fri, 01 Nov 2019 15:59:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 01 Nov 2019 15:59:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX5545245/SRX5545245.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5545245/SRX5545245.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5545245/SRX5545245.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5545245/SRX5545245.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。