Job ID = 1293206 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 45,786,124 reads read : 45,786,124 reads written : 45,786,124 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:33 45786124 reads; of these: 45786124 (100.00%) were unpaired; of these: 6629994 (14.48%) aligned 0 times 32102156 (70.11%) aligned exactly 1 time 7053974 (15.41%) aligned >1 times 85.52% overall alignment rate Time searching: 00:10:33 Overall time: 00:10:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6672455 / 39156130 = 0.1704 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 22:58:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5402758/SRX5402758.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5402758/SRX5402758.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5402758/SRX5402758.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5402758/SRX5402758.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 22:58:51: #1 read tag files... INFO @ Sun, 02 Jun 2019 22:58:51: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 22:58:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5402758/SRX5402758.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5402758/SRX5402758.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5402758/SRX5402758.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5402758/SRX5402758.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 22:58:51: #1 read tag files... INFO @ Sun, 02 Jun 2019 22:58:51: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 22:58:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5402758/SRX5402758.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5402758/SRX5402758.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5402758/SRX5402758.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5402758/SRX5402758.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 22:58:51: #1 read tag files... INFO @ Sun, 02 Jun 2019 22:58:51: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 22:58:59: 1000000 INFO @ Sun, 02 Jun 2019 22:59:00: 1000000 INFO @ Sun, 02 Jun 2019 22:59:01: 1000000 INFO @ Sun, 02 Jun 2019 22:59:06: 2000000 INFO @ Sun, 02 Jun 2019 22:59:08: 2000000 INFO @ Sun, 02 Jun 2019 22:59:10: 2000000 INFO @ Sun, 02 Jun 2019 22:59:14: 3000000 INFO @ Sun, 02 Jun 2019 22:59:17: 3000000 INFO @ Sun, 02 Jun 2019 22:59:19: 3000000 INFO @ Sun, 02 Jun 2019 22:59:21: 4000000 INFO @ Sun, 02 Jun 2019 22:59:25: 4000000 INFO @ Sun, 02 Jun 2019 22:59:28: 4000000 INFO @ Sun, 02 Jun 2019 22:59:29: 5000000 INFO @ Sun, 02 Jun 2019 22:59:33: 5000000 INFO @ Sun, 02 Jun 2019 22:59:36: 6000000 INFO @ Sun, 02 Jun 2019 22:59:37: 5000000 INFO @ Sun, 02 Jun 2019 22:59:41: 6000000 INFO @ Sun, 02 Jun 2019 22:59:43: 7000000 INFO @ Sun, 02 Jun 2019 22:59:46: 6000000 INFO @ Sun, 02 Jun 2019 22:59:49: 7000000 INFO @ Sun, 02 Jun 2019 22:59:50: 8000000 INFO @ Sun, 02 Jun 2019 22:59:55: 7000000 INFO @ Sun, 02 Jun 2019 22:59:57: 8000000 INFO @ Sun, 02 Jun 2019 22:59:57: 9000000 INFO @ Sun, 02 Jun 2019 23:00:04: 8000000 INFO @ Sun, 02 Jun 2019 23:00:04: 10000000 INFO @ Sun, 02 Jun 2019 23:00:05: 9000000 INFO @ Sun, 02 Jun 2019 23:00:12: 11000000 INFO @ Sun, 02 Jun 2019 23:00:13: 10000000 INFO @ Sun, 02 Jun 2019 23:00:13: 9000000 INFO @ Sun, 02 Jun 2019 23:00:19: 12000000 INFO @ Sun, 02 Jun 2019 23:00:22: 11000000 INFO @ Sun, 02 Jun 2019 23:00:22: 10000000 INFO @ Sun, 02 Jun 2019 23:00:26: 13000000 INFO @ Sun, 02 Jun 2019 23:00:29: 12000000 INFO @ Sun, 02 Jun 2019 23:00:32: 11000000 INFO @ Sun, 02 Jun 2019 23:00:34: 14000000 INFO @ Sun, 02 Jun 2019 23:00:38: 13000000 INFO @ Sun, 02 Jun 2019 23:00:41: 12000000 INFO @ Sun, 02 Jun 2019 23:00:41: 15000000 INFO @ Sun, 02 Jun 2019 23:00:46: 14000000 INFO @ Sun, 02 Jun 2019 23:00:48: 16000000 INFO @ Sun, 02 Jun 2019 23:00:50: 13000000 INFO @ Sun, 02 Jun 2019 23:00:54: 15000000 INFO @ Sun, 02 Jun 2019 23:00:55: 17000000 INFO @ Sun, 02 Jun 2019 23:00:59: 14000000 INFO @ Sun, 02 Jun 2019 23:01:02: 16000000 INFO @ Sun, 02 Jun 2019 23:01:02: 18000000 INFO @ Sun, 02 Jun 2019 23:01:08: 15000000 INFO @ Sun, 02 Jun 2019 23:01:09: 19000000 INFO @ Sun, 02 Jun 2019 23:01:10: 17000000 INFO @ Sun, 02 Jun 2019 23:01:16: 20000000 INFO @ Sun, 02 Jun 2019 23:01:17: 16000000 INFO @ Sun, 02 Jun 2019 23:01:18: 18000000 INFO @ Sun, 02 Jun 2019 23:01:24: 21000000 INFO @ Sun, 02 Jun 2019 23:01:26: 19000000 INFO @ Sun, 02 Jun 2019 23:01:26: 17000000 INFO @ Sun, 02 Jun 2019 23:01:31: 22000000 INFO @ Sun, 02 Jun 2019 23:01:34: 20000000 INFO @ Sun, 02 Jun 2019 23:01:35: 18000000 INFO @ Sun, 02 Jun 2019 23:01:38: 23000000 INFO @ Sun, 02 Jun 2019 23:01:42: 21000000 INFO @ Sun, 02 Jun 2019 23:01:44: 19000000 INFO @ Sun, 02 Jun 2019 23:01:46: 24000000 INFO @ Sun, 02 Jun 2019 23:01:50: 22000000 INFO @ Sun, 02 Jun 2019 23:01:53: 20000000 INFO @ Sun, 02 Jun 2019 23:01:54: 25000000 INFO @ Sun, 02 Jun 2019 23:01:59: 23000000 INFO @ Sun, 02 Jun 2019 23:02:01: 26000000 INFO @ Sun, 02 Jun 2019 23:02:02: 21000000 INFO @ Sun, 02 Jun 2019 23:02:06: 24000000 INFO @ Sun, 02 Jun 2019 23:02:08: 27000000 INFO @ Sun, 02 Jun 2019 23:02:11: 22000000 INFO @ Sun, 02 Jun 2019 23:02:13: 25000000 INFO @ Sun, 02 Jun 2019 23:02:16: 28000000 INFO @ Sun, 02 Jun 2019 23:02:20: 23000000 INFO @ Sun, 02 Jun 2019 23:02:21: 26000000 INFO @ Sun, 02 Jun 2019 23:02:23: 29000000 INFO @ Sun, 02 Jun 2019 23:02:28: 24000000 INFO @ Sun, 02 Jun 2019 23:02:29: 27000000 INFO @ Sun, 02 Jun 2019 23:02:31: 30000000 INFO @ Sun, 02 Jun 2019 23:02:36: 25000000 INFO @ Sun, 02 Jun 2019 23:02:38: 28000000 INFO @ Sun, 02 Jun 2019 23:02:38: 31000000 INFO @ Sun, 02 Jun 2019 23:02:45: 32000000 INFO @ Sun, 02 Jun 2019 23:02:45: 26000000 INFO @ Sun, 02 Jun 2019 23:02:46: 29000000 INFO @ Sun, 02 Jun 2019 23:02:48: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 23:02:48: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 23:02:48: #1 total tags in treatment: 32483675 INFO @ Sun, 02 Jun 2019 23:02:48: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 23:02:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 23:02:49: #1 tags after filtering in treatment: 32483675 INFO @ Sun, 02 Jun 2019 23:02:49: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 23:02:49: #1 finished! INFO @ Sun, 02 Jun 2019 23:02:49: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 23:02:49: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 23:02:52: #2 number of paired peaks: 50 WARNING @ Sun, 02 Jun 2019 23:02:52: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 23:02:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX5402758/SRX5402758.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402758/SRX5402758.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402758/SRX5402758.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402758/SRX5402758.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 23:02:54: 27000000 INFO @ Sun, 02 Jun 2019 23:02:54: 30000000 INFO @ Sun, 02 Jun 2019 23:03:02: 31000000 INFO @ Sun, 02 Jun 2019 23:03:04: 28000000 INFO @ Sun, 02 Jun 2019 23:03:10: 32000000 INFO @ Sun, 02 Jun 2019 23:03:13: 29000000 INFO @ Sun, 02 Jun 2019 23:03:14: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 23:03:14: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 23:03:14: #1 total tags in treatment: 32483675 INFO @ Sun, 02 Jun 2019 23:03:14: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 23:03:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 23:03:15: #1 tags after filtering in treatment: 32483675 INFO @ Sun, 02 Jun 2019 23:03:15: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 23:03:15: #1 finished! INFO @ Sun, 02 Jun 2019 23:03:15: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 23:03:15: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 23:03:18: #2 number of paired peaks: 50 WARNING @ Sun, 02 Jun 2019 23:03:18: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 23:03:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX5402758/SRX5402758.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402758/SRX5402758.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402758/SRX5402758.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402758/SRX5402758.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 23:03:21: 30000000 INFO @ Sun, 02 Jun 2019 23:03:29: 31000000 INFO @ Sun, 02 Jun 2019 23:03:37: 32000000 INFO @ Sun, 02 Jun 2019 23:03:42: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 23:03:42: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 23:03:42: #1 total tags in treatment: 32483675 INFO @ Sun, 02 Jun 2019 23:03:42: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 23:03:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 23:03:42: #1 tags after filtering in treatment: 32483675 INFO @ Sun, 02 Jun 2019 23:03:42: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 23:03:42: #1 finished! INFO @ Sun, 02 Jun 2019 23:03:42: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 23:03:42: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 23:03:45: #2 number of paired peaks: 50 WARNING @ Sun, 02 Jun 2019 23:03:45: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 23:03:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX5402758/SRX5402758.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402758/SRX5402758.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402758/SRX5402758.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402758/SRX5402758.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。