Job ID = 1293113 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 8,711,649 reads read : 8,711,649 reads written : 8,711,649 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:29 8711649 reads; of these: 8711649 (100.00%) were unpaired; of these: 197473 (2.27%) aligned 0 times 6910087 (79.32%) aligned exactly 1 time 1604089 (18.41%) aligned >1 times 97.73% overall alignment rate Time searching: 00:02:29 Overall time: 00:02:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 1241897 / 8514176 = 0.1459 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 21:57:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX529224/SRX529224.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX529224/SRX529224.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX529224/SRX529224.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX529224/SRX529224.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 21:57:39: #1 read tag files... INFO @ Sun, 02 Jun 2019 21:57:39: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 21:57:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX529224/SRX529224.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX529224/SRX529224.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX529224/SRX529224.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX529224/SRX529224.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 21:57:39: #1 read tag files... INFO @ Sun, 02 Jun 2019 21:57:39: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 21:57:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX529224/SRX529224.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX529224/SRX529224.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX529224/SRX529224.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX529224/SRX529224.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 21:57:39: #1 read tag files... INFO @ Sun, 02 Jun 2019 21:57:39: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 21:57:48: 1000000 INFO @ Sun, 02 Jun 2019 21:57:48: 1000000 INFO @ Sun, 02 Jun 2019 21:57:48: 1000000 INFO @ Sun, 02 Jun 2019 21:57:56: 2000000 INFO @ Sun, 02 Jun 2019 21:57:56: 2000000 INFO @ Sun, 02 Jun 2019 21:57:57: 2000000 INFO @ Sun, 02 Jun 2019 21:58:04: 3000000 INFO @ Sun, 02 Jun 2019 21:58:04: 3000000 INFO @ Sun, 02 Jun 2019 21:58:05: 3000000 INFO @ Sun, 02 Jun 2019 21:58:12: 4000000 INFO @ Sun, 02 Jun 2019 21:58:12: 4000000 INFO @ Sun, 02 Jun 2019 21:58:13: 4000000 INFO @ Sun, 02 Jun 2019 21:58:19: 5000000 INFO @ Sun, 02 Jun 2019 21:58:20: 5000000 INFO @ Sun, 02 Jun 2019 21:58:22: 5000000 INFO @ Sun, 02 Jun 2019 21:58:27: 6000000 INFO @ Sun, 02 Jun 2019 21:58:28: 6000000 INFO @ Sun, 02 Jun 2019 21:58:30: 6000000 INFO @ Sun, 02 Jun 2019 21:58:34: 7000000 INFO @ Sun, 02 Jun 2019 21:58:36: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 21:58:36: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 21:58:36: #1 total tags in treatment: 7272279 INFO @ Sun, 02 Jun 2019 21:58:36: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 21:58:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 21:58:36: #1 tags after filtering in treatment: 7272279 INFO @ Sun, 02 Jun 2019 21:58:36: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 21:58:36: #1 finished! INFO @ Sun, 02 Jun 2019 21:58:36: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 21:58:36: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 21:58:36: 7000000 INFO @ Sun, 02 Jun 2019 21:58:37: #2 number of paired peaks: 356 WARNING @ Sun, 02 Jun 2019 21:58:37: Fewer paired peaks (356) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 356 pairs to build model! INFO @ Sun, 02 Jun 2019 21:58:37: start model_add_line... INFO @ Sun, 02 Jun 2019 21:58:37: start X-correlation... INFO @ Sun, 02 Jun 2019 21:58:37: end of X-cor INFO @ Sun, 02 Jun 2019 21:58:37: #2 finished! INFO @ Sun, 02 Jun 2019 21:58:37: #2 predicted fragment length is 48 bps INFO @ Sun, 02 Jun 2019 21:58:37: #2 alternative fragment length(s) may be 4,48,567 bps INFO @ Sun, 02 Jun 2019 21:58:37: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX529224/SRX529224.20_model.r WARNING @ Sun, 02 Jun 2019 21:58:37: #2 Since the d (48) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 21:58:37: #2 You may need to consider one of the other alternative d(s): 4,48,567 WARNING @ Sun, 02 Jun 2019 21:58:37: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 21:58:37: #3 Call peaks... INFO @ Sun, 02 Jun 2019 21:58:37: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 21:58:38: 7000000 INFO @ Sun, 02 Jun 2019 21:58:38: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 21:58:39: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 21:58:39: #1 total tags in treatment: 7272279 INFO @ Sun, 02 Jun 2019 21:58:39: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 21:58:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 21:58:39: #1 tags after filtering in treatment: 7272279 INFO @ Sun, 02 Jun 2019 21:58:39: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 21:58:39: #1 finished! INFO @ Sun, 02 Jun 2019 21:58:39: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 21:58:39: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 21:58:39: #2 number of paired peaks: 356 WARNING @ Sun, 02 Jun 2019 21:58:39: Fewer paired peaks (356) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 356 pairs to build model! INFO @ Sun, 02 Jun 2019 21:58:39: start model_add_line... INFO @ Sun, 02 Jun 2019 21:58:39: start X-correlation... INFO @ Sun, 02 Jun 2019 21:58:39: end of X-cor INFO @ Sun, 02 Jun 2019 21:58:39: #2 finished! INFO @ Sun, 02 Jun 2019 21:58:39: #2 predicted fragment length is 48 bps INFO @ Sun, 02 Jun 2019 21:58:39: #2 alternative fragment length(s) may be 4,48,567 bps INFO @ Sun, 02 Jun 2019 21:58:39: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX529224/SRX529224.05_model.r WARNING @ Sun, 02 Jun 2019 21:58:39: #2 Since the d (48) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 21:58:39: #2 You may need to consider one of the other alternative d(s): 4,48,567 WARNING @ Sun, 02 Jun 2019 21:58:39: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 21:58:39: #3 Call peaks... INFO @ Sun, 02 Jun 2019 21:58:39: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 21:58:40: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 21:58:40: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 21:58:40: #1 total tags in treatment: 7272279 INFO @ Sun, 02 Jun 2019 21:58:40: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 21:58:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 21:58:40: #1 tags after filtering in treatment: 7272279 INFO @ Sun, 02 Jun 2019 21:58:40: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 21:58:40: #1 finished! INFO @ Sun, 02 Jun 2019 21:58:40: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 21:58:40: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 21:58:41: #2 number of paired peaks: 356 WARNING @ Sun, 02 Jun 2019 21:58:41: Fewer paired peaks (356) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 356 pairs to build model! INFO @ Sun, 02 Jun 2019 21:58:41: start model_add_line... INFO @ Sun, 02 Jun 2019 21:58:41: start X-correlation... INFO @ Sun, 02 Jun 2019 21:58:41: end of X-cor INFO @ Sun, 02 Jun 2019 21:58:41: #2 finished! INFO @ Sun, 02 Jun 2019 21:58:41: #2 predicted fragment length is 48 bps INFO @ Sun, 02 Jun 2019 21:58:41: #2 alternative fragment length(s) may be 4,48,567 bps INFO @ Sun, 02 Jun 2019 21:58:41: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX529224/SRX529224.10_model.r WARNING @ Sun, 02 Jun 2019 21:58:41: #2 Since the d (48) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 21:58:41: #2 You may need to consider one of the other alternative d(s): 4,48,567 WARNING @ Sun, 02 Jun 2019 21:58:41: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 21:58:41: #3 Call peaks... INFO @ Sun, 02 Jun 2019 21:58:41: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 21:58:58: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 21:59:00: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 21:59:02: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 21:59:08: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX529224/SRX529224.20_peaks.xls INFO @ Sun, 02 Jun 2019 21:59:08: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX529224/SRX529224.20_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 21:59:08: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX529224/SRX529224.20_summits.bed INFO @ Sun, 02 Jun 2019 21:59:08: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (171 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 21:59:10: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX529224/SRX529224.05_peaks.xls INFO @ Sun, 02 Jun 2019 21:59:10: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX529224/SRX529224.05_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 21:59:10: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX529224/SRX529224.05_summits.bed INFO @ Sun, 02 Jun 2019 21:59:10: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (633 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 21:59:12: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX529224/SRX529224.10_peaks.xls INFO @ Sun, 02 Jun 2019 21:59:12: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX529224/SRX529224.10_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 21:59:12: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX529224/SRX529224.10_summits.bed INFO @ Sun, 02 Jun 2019 21:59:12: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (410 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。