Job ID = 6497540 SRX = SRX514728 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T22:37:20 prefetch.2.10.7: 1) Downloading 'SRR1231449'... 2020-06-25T22:37:20 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T22:43:55 prefetch.2.10.7: HTTPS download succeed 2020-06-25T22:43:55 prefetch.2.10.7: 1) 'SRR1231449' was downloaded successfully Read 37371616 spots for SRR1231449/SRR1231449.sra Written 37371616 spots for SRR1231449/SRR1231449.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:33 37371616 reads; of these: 37371616 (100.00%) were unpaired; of these: 195805 (0.52%) aligned 0 times 30716387 (82.19%) aligned exactly 1 time 6459424 (17.28%) aligned >1 times 99.48% overall alignment rate Time searching: 00:08:34 Overall time: 00:08:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7090600 / 37175811 = 0.1907 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:02:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX514728/SRX514728.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX514728/SRX514728.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX514728/SRX514728.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX514728/SRX514728.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:02:59: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:02:59: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:03:06: 1000000 INFO @ Fri, 26 Jun 2020 08:03:12: 2000000 INFO @ Fri, 26 Jun 2020 08:03:18: 3000000 INFO @ Fri, 26 Jun 2020 08:03:25: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:03:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX514728/SRX514728.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX514728/SRX514728.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX514728/SRX514728.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX514728/SRX514728.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:03:29: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:03:29: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:03:31: 5000000 INFO @ Fri, 26 Jun 2020 08:03:36: 1000000 INFO @ Fri, 26 Jun 2020 08:03:37: 6000000 INFO @ Fri, 26 Jun 2020 08:03:43: 2000000 INFO @ Fri, 26 Jun 2020 08:03:44: 7000000 INFO @ Fri, 26 Jun 2020 08:03:49: 3000000 INFO @ Fri, 26 Jun 2020 08:03:51: 8000000 INFO @ Fri, 26 Jun 2020 08:03:56: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:03:57: 9000000 INFO @ Fri, 26 Jun 2020 08:03:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX514728/SRX514728.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX514728/SRX514728.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX514728/SRX514728.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX514728/SRX514728.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:03:59: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:03:59: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:04:03: 5000000 INFO @ Fri, 26 Jun 2020 08:04:04: 10000000 INFO @ Fri, 26 Jun 2020 08:04:07: 1000000 INFO @ Fri, 26 Jun 2020 08:04:09: 6000000 INFO @ Fri, 26 Jun 2020 08:04:11: 11000000 INFO @ Fri, 26 Jun 2020 08:04:14: 2000000 INFO @ Fri, 26 Jun 2020 08:04:16: 7000000 INFO @ Fri, 26 Jun 2020 08:04:18: 12000000 INFO @ Fri, 26 Jun 2020 08:04:21: 3000000 INFO @ Fri, 26 Jun 2020 08:04:23: 8000000 INFO @ Fri, 26 Jun 2020 08:04:25: 13000000 INFO @ Fri, 26 Jun 2020 08:04:28: 4000000 INFO @ Fri, 26 Jun 2020 08:04:29: 9000000 INFO @ Fri, 26 Jun 2020 08:04:32: 14000000 INFO @ Fri, 26 Jun 2020 08:04:35: 5000000 INFO @ Fri, 26 Jun 2020 08:04:36: 10000000 INFO @ Fri, 26 Jun 2020 08:04:39: 15000000 INFO @ Fri, 26 Jun 2020 08:04:42: 6000000 INFO @ Fri, 26 Jun 2020 08:04:43: 11000000 INFO @ Fri, 26 Jun 2020 08:04:46: 16000000 INFO @ Fri, 26 Jun 2020 08:04:49: 7000000 INFO @ Fri, 26 Jun 2020 08:04:50: 12000000 INFO @ Fri, 26 Jun 2020 08:04:52: 17000000 INFO @ Fri, 26 Jun 2020 08:04:55: 8000000 INFO @ Fri, 26 Jun 2020 08:04:57: 13000000 INFO @ Fri, 26 Jun 2020 08:04:59: 18000000 INFO @ Fri, 26 Jun 2020 08:05:02: 9000000 INFO @ Fri, 26 Jun 2020 08:05:03: 14000000 INFO @ Fri, 26 Jun 2020 08:05:06: 19000000 INFO @ Fri, 26 Jun 2020 08:05:09: 10000000 INFO @ Fri, 26 Jun 2020 08:05:10: 15000000 INFO @ Fri, 26 Jun 2020 08:05:13: 20000000 INFO @ Fri, 26 Jun 2020 08:05:16: 11000000 INFO @ Fri, 26 Jun 2020 08:05:16: 16000000 INFO @ Fri, 26 Jun 2020 08:05:19: 21000000 INFO @ Fri, 26 Jun 2020 08:05:22: 12000000 INFO @ Fri, 26 Jun 2020 08:05:23: 17000000 INFO @ Fri, 26 Jun 2020 08:05:26: 22000000 INFO @ Fri, 26 Jun 2020 08:05:29: 18000000 INFO @ Fri, 26 Jun 2020 08:05:29: 13000000 INFO @ Fri, 26 Jun 2020 08:05:33: 23000000 INFO @ Fri, 26 Jun 2020 08:05:36: 19000000 INFO @ Fri, 26 Jun 2020 08:05:36: 14000000 INFO @ Fri, 26 Jun 2020 08:05:40: 24000000 INFO @ Fri, 26 Jun 2020 08:05:43: 20000000 INFO @ Fri, 26 Jun 2020 08:05:43: 15000000 INFO @ Fri, 26 Jun 2020 08:05:47: 25000000 INFO @ Fri, 26 Jun 2020 08:05:50: 21000000 INFO @ Fri, 26 Jun 2020 08:05:50: 16000000 INFO @ Fri, 26 Jun 2020 08:05:54: 26000000 INFO @ Fri, 26 Jun 2020 08:05:57: 22000000 INFO @ Fri, 26 Jun 2020 08:05:57: 17000000 INFO @ Fri, 26 Jun 2020 08:06:01: 27000000 INFO @ Fri, 26 Jun 2020 08:06:03: 23000000 INFO @ Fri, 26 Jun 2020 08:06:04: 18000000 INFO @ Fri, 26 Jun 2020 08:06:08: 28000000 INFO @ Fri, 26 Jun 2020 08:06:10: 24000000 INFO @ Fri, 26 Jun 2020 08:06:11: 19000000 INFO @ Fri, 26 Jun 2020 08:06:15: 29000000 INFO @ Fri, 26 Jun 2020 08:06:17: 25000000 INFO @ Fri, 26 Jun 2020 08:06:18: 20000000 INFO @ Fri, 26 Jun 2020 08:06:22: 30000000 INFO @ Fri, 26 Jun 2020 08:06:23: #1 tag size is determined as 42 bps INFO @ Fri, 26 Jun 2020 08:06:23: #1 tag size = 42 INFO @ Fri, 26 Jun 2020 08:06:23: #1 total tags in treatment: 30085211 INFO @ Fri, 26 Jun 2020 08:06:23: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:06:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:06:23: #1 tags after filtering in treatment: 30085211 INFO @ Fri, 26 Jun 2020 08:06:23: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:06:23: #1 finished! INFO @ Fri, 26 Jun 2020 08:06:23: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:06:23: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:06:24: 26000000 INFO @ Fri, 26 Jun 2020 08:06:24: 21000000 INFO @ Fri, 26 Jun 2020 08:06:25: #2 number of paired peaks: 83 WARNING @ Fri, 26 Jun 2020 08:06:25: Too few paired peaks (83) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:06:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX514728/SRX514728.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX514728/SRX514728.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX514728/SRX514728.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX514728/SRX514728.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 08:06:30: 27000000 INFO @ Fri, 26 Jun 2020 08:06:31: 22000000 INFO @ Fri, 26 Jun 2020 08:06:37: 28000000 INFO @ Fri, 26 Jun 2020 08:06:38: 23000000 INFO @ Fri, 26 Jun 2020 08:06:43: 29000000 INFO @ Fri, 26 Jun 2020 08:06:44: 24000000 INFO @ Fri, 26 Jun 2020 08:06:50: 30000000 INFO @ Fri, 26 Jun 2020 08:06:51: #1 tag size is determined as 42 bps INFO @ Fri, 26 Jun 2020 08:06:51: #1 tag size = 42 INFO @ Fri, 26 Jun 2020 08:06:51: #1 total tags in treatment: 30085211 INFO @ Fri, 26 Jun 2020 08:06:51: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:06:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:06:51: 25000000 INFO @ Fri, 26 Jun 2020 08:06:51: #1 tags after filtering in treatment: 30085211 INFO @ Fri, 26 Jun 2020 08:06:51: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:06:51: #1 finished! INFO @ Fri, 26 Jun 2020 08:06:51: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:06:51: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:06:53: #2 number of paired peaks: 83 WARNING @ Fri, 26 Jun 2020 08:06:53: Too few paired peaks (83) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:06:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX514728/SRX514728.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX514728/SRX514728.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX514728/SRX514728.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX514728/SRX514728.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:06:57: 26000000 INFO @ Fri, 26 Jun 2020 08:07:04: 27000000 INFO @ Fri, 26 Jun 2020 08:07:10: 28000000 INFO @ Fri, 26 Jun 2020 08:07:16: 29000000 INFO @ Fri, 26 Jun 2020 08:07:22: 30000000 INFO @ Fri, 26 Jun 2020 08:07:23: #1 tag size is determined as 42 bps INFO @ Fri, 26 Jun 2020 08:07:23: #1 tag size = 42 INFO @ Fri, 26 Jun 2020 08:07:23: #1 total tags in treatment: 30085211 INFO @ Fri, 26 Jun 2020 08:07:23: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:07:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:07:24: #1 tags after filtering in treatment: 30085211 INFO @ Fri, 26 Jun 2020 08:07:24: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:07:24: #1 finished! INFO @ Fri, 26 Jun 2020 08:07:24: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:07:24: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:07:26: #2 number of paired peaks: 83 WARNING @ Fri, 26 Jun 2020 08:07:26: Too few paired peaks (83) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:07:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX514728/SRX514728.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX514728/SRX514728.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX514728/SRX514728.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX514728/SRX514728.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。