Job ID = 1293049 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 11,689,760 reads read : 11,689,760 reads written : 11,689,760 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:58 11689760 reads; of these: 11689760 (100.00%) were unpaired; of these: 79909 (0.68%) aligned 0 times 9633772 (82.41%) aligned exactly 1 time 1976079 (16.90%) aligned >1 times 99.32% overall alignment rate Time searching: 00:02:58 Overall time: 00:02:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 879472 / 11609851 = 0.0758 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 21:37:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5020813/SRX5020813.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5020813/SRX5020813.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5020813/SRX5020813.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5020813/SRX5020813.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 21:37:25: #1 read tag files... INFO @ Sun, 02 Jun 2019 21:37:25: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 21:37:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5020813/SRX5020813.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5020813/SRX5020813.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5020813/SRX5020813.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5020813/SRX5020813.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 21:37:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5020813/SRX5020813.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5020813/SRX5020813.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5020813/SRX5020813.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5020813/SRX5020813.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 21:37:25: #1 read tag files... INFO @ Sun, 02 Jun 2019 21:37:25: #1 read tag files... INFO @ Sun, 02 Jun 2019 21:37:25: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 21:37:25: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 21:37:34: 1000000 INFO @ Sun, 02 Jun 2019 21:37:34: 1000000 INFO @ Sun, 02 Jun 2019 21:37:35: 1000000 INFO @ Sun, 02 Jun 2019 21:37:42: 2000000 INFO @ Sun, 02 Jun 2019 21:37:43: 2000000 INFO @ Sun, 02 Jun 2019 21:37:44: 2000000 INFO @ Sun, 02 Jun 2019 21:37:50: 3000000 INFO @ Sun, 02 Jun 2019 21:37:52: 3000000 INFO @ Sun, 02 Jun 2019 21:37:53: 3000000 INFO @ Sun, 02 Jun 2019 21:37:58: 4000000 INFO @ Sun, 02 Jun 2019 21:38:01: 4000000 INFO @ Sun, 02 Jun 2019 21:38:02: 4000000 INFO @ Sun, 02 Jun 2019 21:38:06: 5000000 INFO @ Sun, 02 Jun 2019 21:38:09: 5000000 INFO @ Sun, 02 Jun 2019 21:38:11: 5000000 INFO @ Sun, 02 Jun 2019 21:38:14: 6000000 INFO @ Sun, 02 Jun 2019 21:38:18: 6000000 INFO @ Sun, 02 Jun 2019 21:38:20: 6000000 INFO @ Sun, 02 Jun 2019 21:38:22: 7000000 INFO @ Sun, 02 Jun 2019 21:38:27: 7000000 INFO @ Sun, 02 Jun 2019 21:38:30: 7000000 INFO @ Sun, 02 Jun 2019 21:38:31: 8000000 INFO @ Sun, 02 Jun 2019 21:38:36: 8000000 INFO @ Sun, 02 Jun 2019 21:38:39: 8000000 INFO @ Sun, 02 Jun 2019 21:38:40: 9000000 INFO @ Sun, 02 Jun 2019 21:38:45: 9000000 INFO @ Sun, 02 Jun 2019 21:38:48: 10000000 INFO @ Sun, 02 Jun 2019 21:38:48: 9000000 INFO @ Sun, 02 Jun 2019 21:38:54: #1 tag size is determined as 51 bps INFO @ Sun, 02 Jun 2019 21:38:54: #1 tag size = 51 INFO @ Sun, 02 Jun 2019 21:38:54: #1 total tags in treatment: 10730379 INFO @ Sun, 02 Jun 2019 21:38:54: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 21:38:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 21:38:54: #1 tags after filtering in treatment: 10730379 INFO @ Sun, 02 Jun 2019 21:38:54: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 21:38:54: #1 finished! INFO @ Sun, 02 Jun 2019 21:38:54: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 21:38:54: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 21:38:54: 10000000 INFO @ Sun, 02 Jun 2019 21:38:55: #2 number of paired peaks: 325 WARNING @ Sun, 02 Jun 2019 21:38:55: Fewer paired peaks (325) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 325 pairs to build model! INFO @ Sun, 02 Jun 2019 21:38:55: start model_add_line... INFO @ Sun, 02 Jun 2019 21:38:55: start X-correlation... INFO @ Sun, 02 Jun 2019 21:38:55: end of X-cor INFO @ Sun, 02 Jun 2019 21:38:55: #2 finished! INFO @ Sun, 02 Jun 2019 21:38:55: #2 predicted fragment length is 50 bps INFO @ Sun, 02 Jun 2019 21:38:55: #2 alternative fragment length(s) may be 3,50,581 bps INFO @ Sun, 02 Jun 2019 21:38:55: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX5020813/SRX5020813.05_model.r WARNING @ Sun, 02 Jun 2019 21:38:55: #2 Since the d (50) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 21:38:55: #2 You may need to consider one of the other alternative d(s): 3,50,581 WARNING @ Sun, 02 Jun 2019 21:38:55: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 21:38:55: #3 Call peaks... INFO @ Sun, 02 Jun 2019 21:38:55: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 21:38:57: 10000000 INFO @ Sun, 02 Jun 2019 21:39:00: #1 tag size is determined as 51 bps INFO @ Sun, 02 Jun 2019 21:39:00: #1 tag size = 51 INFO @ Sun, 02 Jun 2019 21:39:00: #1 total tags in treatment: 10730379 INFO @ Sun, 02 Jun 2019 21:39:00: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 21:39:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 21:39:00: #1 tags after filtering in treatment: 10730379 INFO @ Sun, 02 Jun 2019 21:39:00: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 21:39:00: #1 finished! INFO @ Sun, 02 Jun 2019 21:39:00: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 21:39:00: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 21:39:01: #2 number of paired peaks: 325 WARNING @ Sun, 02 Jun 2019 21:39:01: Fewer paired peaks (325) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 325 pairs to build model! INFO @ Sun, 02 Jun 2019 21:39:01: start model_add_line... INFO @ Sun, 02 Jun 2019 21:39:01: start X-correlation... INFO @ Sun, 02 Jun 2019 21:39:01: end of X-cor INFO @ Sun, 02 Jun 2019 21:39:01: #2 finished! INFO @ Sun, 02 Jun 2019 21:39:01: #2 predicted fragment length is 50 bps INFO @ Sun, 02 Jun 2019 21:39:01: #2 alternative fragment length(s) may be 3,50,581 bps INFO @ Sun, 02 Jun 2019 21:39:01: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX5020813/SRX5020813.10_model.r WARNING @ Sun, 02 Jun 2019 21:39:01: #2 Since the d (50) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 21:39:01: #2 You may need to consider one of the other alternative d(s): 3,50,581 WARNING @ Sun, 02 Jun 2019 21:39:01: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 21:39:01: #3 Call peaks... INFO @ Sun, 02 Jun 2019 21:39:01: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 21:39:04: #1 tag size is determined as 51 bps INFO @ Sun, 02 Jun 2019 21:39:04: #1 tag size = 51 INFO @ Sun, 02 Jun 2019 21:39:04: #1 total tags in treatment: 10730379 INFO @ Sun, 02 Jun 2019 21:39:04: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 21:39:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 21:39:04: #1 tags after filtering in treatment: 10730379 INFO @ Sun, 02 Jun 2019 21:39:04: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 21:39:04: #1 finished! INFO @ Sun, 02 Jun 2019 21:39:04: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 21:39:04: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 21:39:05: #2 number of paired peaks: 325 WARNING @ Sun, 02 Jun 2019 21:39:05: Fewer paired peaks (325) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 325 pairs to build model! INFO @ Sun, 02 Jun 2019 21:39:05: start model_add_line... INFO @ Sun, 02 Jun 2019 21:39:05: start X-correlation... INFO @ Sun, 02 Jun 2019 21:39:05: end of X-cor INFO @ Sun, 02 Jun 2019 21:39:05: #2 finished! INFO @ Sun, 02 Jun 2019 21:39:05: #2 predicted fragment length is 50 bps INFO @ Sun, 02 Jun 2019 21:39:05: #2 alternative fragment length(s) may be 3,50,581 bps INFO @ Sun, 02 Jun 2019 21:39:05: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX5020813/SRX5020813.20_model.r WARNING @ Sun, 02 Jun 2019 21:39:05: #2 Since the d (50) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 21:39:05: #2 You may need to consider one of the other alternative d(s): 3,50,581 WARNING @ Sun, 02 Jun 2019 21:39:05: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 21:39:05: #3 Call peaks... INFO @ Sun, 02 Jun 2019 21:39:05: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 21:39:23: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 21:39:30: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 21:39:34: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 21:39:37: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX5020813/SRX5020813.05_peaks.xls INFO @ Sun, 02 Jun 2019 21:39:37: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX5020813/SRX5020813.05_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 21:39:37: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX5020813/SRX5020813.05_summits.bed INFO @ Sun, 02 Jun 2019 21:39:37: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (628 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 21:39:45: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX5020813/SRX5020813.10_peaks.xls INFO @ Sun, 02 Jun 2019 21:39:45: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX5020813/SRX5020813.10_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 21:39:45: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX5020813/SRX5020813.10_summits.bed INFO @ Sun, 02 Jun 2019 21:39:45: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (413 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 21:39:48: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX5020813/SRX5020813.20_peaks.xls INFO @ Sun, 02 Jun 2019 21:39:48: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX5020813/SRX5020813.20_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 21:39:48: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX5020813/SRX5020813.20_summits.bed INFO @ Sun, 02 Jun 2019 21:39:48: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (165 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。