Job ID = 1292985 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 15,276,123 reads read : 30,552,246 reads written : 15,276,123 reads 0-length : 15,276,123 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:39 15276123 reads; of these: 15276123 (100.00%) were unpaired; of these: 3564546 (23.33%) aligned 0 times 9763568 (63.91%) aligned exactly 1 time 1948009 (12.75%) aligned >1 times 76.67% overall alignment rate Time searching: 00:05:39 Overall time: 00:05:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1520521 / 11711577 = 0.1298 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 21:29:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5020761/SRX5020761.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5020761/SRX5020761.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5020761/SRX5020761.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5020761/SRX5020761.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 21:29:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5020761/SRX5020761.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5020761/SRX5020761.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5020761/SRX5020761.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5020761/SRX5020761.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 21:29:48: #1 read tag files... INFO @ Sun, 02 Jun 2019 21:29:48: #1 read tag files... INFO @ Sun, 02 Jun 2019 21:29:48: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 21:29:48: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 21:29:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5020761/SRX5020761.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5020761/SRX5020761.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5020761/SRX5020761.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5020761/SRX5020761.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 21:29:48: #1 read tag files... INFO @ Sun, 02 Jun 2019 21:29:48: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 21:29:56: 1000000 INFO @ Sun, 02 Jun 2019 21:29:57: 1000000 INFO @ Sun, 02 Jun 2019 21:29:57: 1000000 INFO @ Sun, 02 Jun 2019 21:30:04: 2000000 INFO @ Sun, 02 Jun 2019 21:30:06: 2000000 INFO @ Sun, 02 Jun 2019 21:30:06: 2000000 INFO @ Sun, 02 Jun 2019 21:30:11: 3000000 INFO @ Sun, 02 Jun 2019 21:30:15: 3000000 INFO @ Sun, 02 Jun 2019 21:30:15: 3000000 INFO @ Sun, 02 Jun 2019 21:30:19: 4000000 INFO @ Sun, 02 Jun 2019 21:30:24: 4000000 INFO @ Sun, 02 Jun 2019 21:30:24: 4000000 INFO @ Sun, 02 Jun 2019 21:30:30: 5000000 INFO @ Sun, 02 Jun 2019 21:30:35: 5000000 INFO @ Sun, 02 Jun 2019 21:30:35: 5000000 INFO @ Sun, 02 Jun 2019 21:30:41: 6000000 INFO @ Sun, 02 Jun 2019 21:30:46: 6000000 INFO @ Sun, 02 Jun 2019 21:30:46: 6000000 INFO @ Sun, 02 Jun 2019 21:30:51: 7000000 INFO @ Sun, 02 Jun 2019 21:30:57: 7000000 INFO @ Sun, 02 Jun 2019 21:30:57: 7000000 INFO @ Sun, 02 Jun 2019 21:31:02: 8000000 INFO @ Sun, 02 Jun 2019 21:31:07: 8000000 INFO @ Sun, 02 Jun 2019 21:31:07: 8000000 INFO @ Sun, 02 Jun 2019 21:31:13: 9000000 INFO @ Sun, 02 Jun 2019 21:31:18: 9000000 INFO @ Sun, 02 Jun 2019 21:31:18: 9000000 INFO @ Sun, 02 Jun 2019 21:31:23: 10000000 INFO @ Sun, 02 Jun 2019 21:31:25: #1 tag size is determined as 75 bps INFO @ Sun, 02 Jun 2019 21:31:25: #1 tag size = 75 INFO @ Sun, 02 Jun 2019 21:31:25: #1 total tags in treatment: 10191056 INFO @ Sun, 02 Jun 2019 21:31:25: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 21:31:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 21:31:25: #1 tags after filtering in treatment: 10191056 INFO @ Sun, 02 Jun 2019 21:31:25: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 21:31:25: #1 finished! INFO @ Sun, 02 Jun 2019 21:31:25: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 21:31:25: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 21:31:26: #2 number of paired peaks: 462 WARNING @ Sun, 02 Jun 2019 21:31:26: Fewer paired peaks (462) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 462 pairs to build model! INFO @ Sun, 02 Jun 2019 21:31:26: start model_add_line... INFO @ Sun, 02 Jun 2019 21:31:27: start X-correlation... INFO @ Sun, 02 Jun 2019 21:31:27: end of X-cor INFO @ Sun, 02 Jun 2019 21:31:27: #2 finished! INFO @ Sun, 02 Jun 2019 21:31:27: #2 predicted fragment length is 94 bps INFO @ Sun, 02 Jun 2019 21:31:27: #2 alternative fragment length(s) may be 94 bps INFO @ Sun, 02 Jun 2019 21:31:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX5020761/SRX5020761.05_model.r WARNING @ Sun, 02 Jun 2019 21:31:27: #2 Since the d (94) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 21:31:27: #2 You may need to consider one of the other alternative d(s): 94 WARNING @ Sun, 02 Jun 2019 21:31:27: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 21:31:27: #3 Call peaks... INFO @ Sun, 02 Jun 2019 21:31:27: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 21:31:28: 10000000 INFO @ Sun, 02 Jun 2019 21:31:28: 10000000 INFO @ Sun, 02 Jun 2019 21:31:30: #1 tag size is determined as 75 bps INFO @ Sun, 02 Jun 2019 21:31:30: #1 tag size = 75 INFO @ Sun, 02 Jun 2019 21:31:30: #1 total tags in treatment: 10191056 INFO @ Sun, 02 Jun 2019 21:31:30: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 21:31:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 21:31:30: #1 tag size is determined as 75 bps INFO @ Sun, 02 Jun 2019 21:31:30: #1 tag size = 75 INFO @ Sun, 02 Jun 2019 21:31:30: #1 total tags in treatment: 10191056 INFO @ Sun, 02 Jun 2019 21:31:30: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 21:31:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 21:31:30: #1 tags after filtering in treatment: 10191056 INFO @ Sun, 02 Jun 2019 21:31:30: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 21:31:30: #1 finished! INFO @ Sun, 02 Jun 2019 21:31:30: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 21:31:30: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 21:31:30: #1 tags after filtering in treatment: 10191056 INFO @ Sun, 02 Jun 2019 21:31:30: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 21:31:30: #1 finished! INFO @ Sun, 02 Jun 2019 21:31:30: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 21:31:30: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 21:31:31: #2 number of paired peaks: 462 WARNING @ Sun, 02 Jun 2019 21:31:31: Fewer paired peaks (462) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 462 pairs to build model! INFO @ Sun, 02 Jun 2019 21:31:31: start model_add_line... INFO @ Sun, 02 Jun 2019 21:31:31: #2 number of paired peaks: 462 WARNING @ Sun, 02 Jun 2019 21:31:31: Fewer paired peaks (462) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 462 pairs to build model! INFO @ Sun, 02 Jun 2019 21:31:31: start model_add_line... INFO @ Sun, 02 Jun 2019 21:31:31: start X-correlation... INFO @ Sun, 02 Jun 2019 21:31:31: start X-correlation... INFO @ Sun, 02 Jun 2019 21:31:31: end of X-cor INFO @ Sun, 02 Jun 2019 21:31:31: #2 finished! INFO @ Sun, 02 Jun 2019 21:31:31: #2 predicted fragment length is 94 bps INFO @ Sun, 02 Jun 2019 21:31:31: #2 alternative fragment length(s) may be 94 bps INFO @ Sun, 02 Jun 2019 21:31:31: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX5020761/SRX5020761.20_model.r INFO @ Sun, 02 Jun 2019 21:31:31: end of X-cor INFO @ Sun, 02 Jun 2019 21:31:31: #2 finished! INFO @ Sun, 02 Jun 2019 21:31:31: #2 predicted fragment length is 94 bps INFO @ Sun, 02 Jun 2019 21:31:31: #2 alternative fragment length(s) may be 94 bps INFO @ Sun, 02 Jun 2019 21:31:31: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX5020761/SRX5020761.10_model.r WARNING @ Sun, 02 Jun 2019 21:31:31: #2 Since the d (94) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 21:31:31: #2 You may need to consider one of the other alternative d(s): 94 WARNING @ Sun, 02 Jun 2019 21:31:31: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 21:31:31: #3 Call peaks... INFO @ Sun, 02 Jun 2019 21:31:31: #3 Pre-compute pvalue-qvalue table... WARNING @ Sun, 02 Jun 2019 21:31:31: #2 Since the d (94) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 21:31:31: #2 You may need to consider one of the other alternative d(s): 94 WARNING @ Sun, 02 Jun 2019 21:31:31: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 21:31:31: #3 Call peaks... INFO @ Sun, 02 Jun 2019 21:31:31: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 21:31:55: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 21:31:59: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 21:31:59: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 21:32:09: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX5020761/SRX5020761.05_peaks.xls INFO @ Sun, 02 Jun 2019 21:32:09: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX5020761/SRX5020761.05_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 21:32:09: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX5020761/SRX5020761.05_summits.bed INFO @ Sun, 02 Jun 2019 21:32:09: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (1679 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 21:32:13: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX5020761/SRX5020761.20_peaks.xls INFO @ Sun, 02 Jun 2019 21:32:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX5020761/SRX5020761.20_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 21:32:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX5020761/SRX5020761.20_summits.bed INFO @ Sun, 02 Jun 2019 21:32:13: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (584 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 21:32:13: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX5020761/SRX5020761.10_peaks.xls INFO @ Sun, 02 Jun 2019 21:32:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX5020761/SRX5020761.10_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 21:32:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX5020761/SRX5020761.10_summits.bed INFO @ Sun, 02 Jun 2019 21:32:13: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (1016 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。