Job ID = 1292969 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 5,568,148 reads read : 5,568,148 reads written : 5,568,148 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:19 5568148 reads; of these: 5568148 (100.00%) were unpaired; of these: 100839 (1.81%) aligned 0 times 4595089 (82.52%) aligned exactly 1 time 872220 (15.66%) aligned >1 times 98.19% overall alignment rate Time searching: 00:01:19 Overall time: 00:01:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 268020 / 5467309 = 0.0490 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 21:15:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5020749/SRX5020749.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5020749/SRX5020749.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5020749/SRX5020749.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5020749/SRX5020749.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 21:15:57: #1 read tag files... INFO @ Sun, 02 Jun 2019 21:15:57: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 21:15:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5020749/SRX5020749.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5020749/SRX5020749.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5020749/SRX5020749.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5020749/SRX5020749.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 21:15:57: #1 read tag files... INFO @ Sun, 02 Jun 2019 21:15:57: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 21:15:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5020749/SRX5020749.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5020749/SRX5020749.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5020749/SRX5020749.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5020749/SRX5020749.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 21:15:57: #1 read tag files... INFO @ Sun, 02 Jun 2019 21:15:57: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 21:16:06: 1000000 INFO @ Sun, 02 Jun 2019 21:16:06: 1000000 INFO @ Sun, 02 Jun 2019 21:16:07: 1000000 INFO @ Sun, 02 Jun 2019 21:16:15: 2000000 INFO @ Sun, 02 Jun 2019 21:16:15: 2000000 INFO @ Sun, 02 Jun 2019 21:16:17: 2000000 INFO @ Sun, 02 Jun 2019 21:16:23: 3000000 INFO @ Sun, 02 Jun 2019 21:16:23: 3000000 INFO @ Sun, 02 Jun 2019 21:16:27: 3000000 INFO @ Sun, 02 Jun 2019 21:16:31: 4000000 INFO @ Sun, 02 Jun 2019 21:16:31: 4000000 INFO @ Sun, 02 Jun 2019 21:16:36: 4000000 INFO @ Sun, 02 Jun 2019 21:16:39: 5000000 INFO @ Sun, 02 Jun 2019 21:16:39: 5000000 INFO @ Sun, 02 Jun 2019 21:16:41: #1 tag size is determined as 51 bps INFO @ Sun, 02 Jun 2019 21:16:41: #1 tag size = 51 INFO @ Sun, 02 Jun 2019 21:16:41: #1 total tags in treatment: 5199289 INFO @ Sun, 02 Jun 2019 21:16:41: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 21:16:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 21:16:41: #1 tags after filtering in treatment: 5199289 INFO @ Sun, 02 Jun 2019 21:16:41: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 21:16:41: #1 finished! INFO @ Sun, 02 Jun 2019 21:16:41: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 21:16:41: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 21:16:41: #1 tag size is determined as 51 bps INFO @ Sun, 02 Jun 2019 21:16:41: #1 tag size = 51 INFO @ Sun, 02 Jun 2019 21:16:41: #1 total tags in treatment: 5199289 INFO @ Sun, 02 Jun 2019 21:16:41: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 21:16:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 21:16:41: #1 tags after filtering in treatment: 5199289 INFO @ Sun, 02 Jun 2019 21:16:41: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 21:16:41: #1 finished! INFO @ Sun, 02 Jun 2019 21:16:41: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 21:16:41: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 21:16:41: #2 number of paired peaks: 335 WARNING @ Sun, 02 Jun 2019 21:16:41: Fewer paired peaks (335) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 335 pairs to build model! INFO @ Sun, 02 Jun 2019 21:16:41: start model_add_line... INFO @ Sun, 02 Jun 2019 21:16:41: start X-correlation... INFO @ Sun, 02 Jun 2019 21:16:41: end of X-cor INFO @ Sun, 02 Jun 2019 21:16:41: #2 finished! INFO @ Sun, 02 Jun 2019 21:16:41: #2 predicted fragment length is 54 bps INFO @ Sun, 02 Jun 2019 21:16:41: #2 alternative fragment length(s) may be 54,571 bps INFO @ Sun, 02 Jun 2019 21:16:41: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX5020749/SRX5020749.10_model.r WARNING @ Sun, 02 Jun 2019 21:16:41: #2 Since the d (54) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 21:16:41: #2 You may need to consider one of the other alternative d(s): 54,571 WARNING @ Sun, 02 Jun 2019 21:16:41: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 21:16:41: #3 Call peaks... INFO @ Sun, 02 Jun 2019 21:16:41: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 21:16:41: #2 number of paired peaks: 335 WARNING @ Sun, 02 Jun 2019 21:16:41: Fewer paired peaks (335) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 335 pairs to build model! INFO @ Sun, 02 Jun 2019 21:16:41: start model_add_line... INFO @ Sun, 02 Jun 2019 21:16:42: start X-correlation... INFO @ Sun, 02 Jun 2019 21:16:42: end of X-cor INFO @ Sun, 02 Jun 2019 21:16:42: #2 finished! INFO @ Sun, 02 Jun 2019 21:16:42: #2 predicted fragment length is 54 bps INFO @ Sun, 02 Jun 2019 21:16:42: #2 alternative fragment length(s) may be 54,571 bps INFO @ Sun, 02 Jun 2019 21:16:42: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX5020749/SRX5020749.05_model.r WARNING @ Sun, 02 Jun 2019 21:16:42: #2 Since the d (54) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 21:16:42: #2 You may need to consider one of the other alternative d(s): 54,571 WARNING @ Sun, 02 Jun 2019 21:16:42: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 21:16:42: #3 Call peaks... INFO @ Sun, 02 Jun 2019 21:16:42: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 21:16:46: 5000000 INFO @ Sun, 02 Jun 2019 21:16:47: #1 tag size is determined as 51 bps INFO @ Sun, 02 Jun 2019 21:16:47: #1 tag size = 51 INFO @ Sun, 02 Jun 2019 21:16:47: #1 total tags in treatment: 5199289 INFO @ Sun, 02 Jun 2019 21:16:47: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 21:16:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 21:16:48: #1 tags after filtering in treatment: 5199289 INFO @ Sun, 02 Jun 2019 21:16:48: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 21:16:48: #1 finished! INFO @ Sun, 02 Jun 2019 21:16:48: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 21:16:48: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 21:16:48: #2 number of paired peaks: 335 WARNING @ Sun, 02 Jun 2019 21:16:48: Fewer paired peaks (335) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 335 pairs to build model! INFO @ Sun, 02 Jun 2019 21:16:48: start model_add_line... INFO @ Sun, 02 Jun 2019 21:16:48: start X-correlation... INFO @ Sun, 02 Jun 2019 21:16:48: end of X-cor INFO @ Sun, 02 Jun 2019 21:16:48: #2 finished! INFO @ Sun, 02 Jun 2019 21:16:48: #2 predicted fragment length is 54 bps INFO @ Sun, 02 Jun 2019 21:16:48: #2 alternative fragment length(s) may be 54,571 bps INFO @ Sun, 02 Jun 2019 21:16:48: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX5020749/SRX5020749.20_model.r WARNING @ Sun, 02 Jun 2019 21:16:48: #2 Since the d (54) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 21:16:48: #2 You may need to consider one of the other alternative d(s): 54,571 WARNING @ Sun, 02 Jun 2019 21:16:48: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 21:16:48: #3 Call peaks... INFO @ Sun, 02 Jun 2019 21:16:48: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 21:16:57: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 21:16:57: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 21:17:03: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 21:17:04: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX5020749/SRX5020749.05_peaks.xls INFO @ Sun, 02 Jun 2019 21:17:04: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX5020749/SRX5020749.05_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 21:17:04: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX5020749/SRX5020749.05_summits.bed INFO @ Sun, 02 Jun 2019 21:17:04: Done! INFO @ Sun, 02 Jun 2019 21:17:04: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX5020749/SRX5020749.10_peaks.xls INFO @ Sun, 02 Jun 2019 21:17:04: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX5020749/SRX5020749.10_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 21:17:04: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX5020749/SRX5020749.10_summits.bed INFO @ Sun, 02 Jun 2019 21:17:04: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (403 records, 4 fields): 3 millis CompletedMACS2peakCalling pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (233 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 21:17:11: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX5020749/SRX5020749.20_peaks.xls INFO @ Sun, 02 Jun 2019 21:17:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX5020749/SRX5020749.20_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 21:17:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX5020749/SRX5020749.20_summits.bed INFO @ Sun, 02 Jun 2019 21:17:11: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (92 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。