Job ID = 2590186 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 47,950,615 reads read : 47,950,615 reads written : 47,950,615 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1198610.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:46 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3139817 (6.55%) aligned 0 times 37484929 (78.17%) aligned exactly 1 time 7325869 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:11:46 Overall time: 00:11:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8188484 / 44810798 = 0.1827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 20:23:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX495078/SRX495078.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX495078/SRX495078.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX495078/SRX495078.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX495078/SRX495078.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 20:23:26: #1 read tag files... INFO @ Mon, 12 Aug 2019 20:23:26: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 20:23:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX495078/SRX495078.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX495078/SRX495078.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX495078/SRX495078.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX495078/SRX495078.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 20:23:27: #1 read tag files... INFO @ Mon, 12 Aug 2019 20:23:27: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 20:23:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX495078/SRX495078.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX495078/SRX495078.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX495078/SRX495078.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX495078/SRX495078.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 20:23:28: #1 read tag files... INFO @ Mon, 12 Aug 2019 20:23:28: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 20:23:36: 1000000 INFO @ Mon, 12 Aug 2019 20:23:37: 1000000 INFO @ Mon, 12 Aug 2019 20:23:37: 1000000 INFO @ Mon, 12 Aug 2019 20:23:45: 2000000 INFO @ Mon, 12 Aug 2019 20:23:46: 2000000 INFO @ Mon, 12 Aug 2019 20:23:47: 2000000 INFO @ Mon, 12 Aug 2019 20:23:53: 3000000 INFO @ Mon, 12 Aug 2019 20:23:54: 3000000 INFO @ Mon, 12 Aug 2019 20:23:56: 3000000 INFO @ Mon, 12 Aug 2019 20:24:01: 4000000 INFO @ Mon, 12 Aug 2019 20:24:02: 4000000 INFO @ Mon, 12 Aug 2019 20:24:04: 4000000 INFO @ Mon, 12 Aug 2019 20:24:09: 5000000 INFO @ Mon, 12 Aug 2019 20:24:10: 5000000 INFO @ Mon, 12 Aug 2019 20:24:13: 5000000 INFO @ Mon, 12 Aug 2019 20:24:16: 6000000 INFO @ Mon, 12 Aug 2019 20:24:18: 6000000 INFO @ Mon, 12 Aug 2019 20:24:22: 6000000 INFO @ Mon, 12 Aug 2019 20:24:24: 7000000 INFO @ Mon, 12 Aug 2019 20:24:26: 7000000 INFO @ Mon, 12 Aug 2019 20:24:30: 7000000 INFO @ Mon, 12 Aug 2019 20:24:32: 8000000 INFO @ Mon, 12 Aug 2019 20:24:34: 8000000 INFO @ Mon, 12 Aug 2019 20:24:39: 8000000 INFO @ Mon, 12 Aug 2019 20:24:40: 9000000 INFO @ Mon, 12 Aug 2019 20:24:42: 9000000 INFO @ Mon, 12 Aug 2019 20:24:48: 9000000 INFO @ Mon, 12 Aug 2019 20:24:48: 10000000 INFO @ Mon, 12 Aug 2019 20:24:50: 10000000 INFO @ Mon, 12 Aug 2019 20:24:56: 11000000 INFO @ Mon, 12 Aug 2019 20:24:57: 10000000 INFO @ Mon, 12 Aug 2019 20:24:58: 11000000 INFO @ Mon, 12 Aug 2019 20:25:04: 12000000 INFO @ Mon, 12 Aug 2019 20:25:05: 11000000 INFO @ Mon, 12 Aug 2019 20:25:06: 12000000 INFO @ Mon, 12 Aug 2019 20:25:12: 13000000 INFO @ Mon, 12 Aug 2019 20:25:13: 13000000 INFO @ Mon, 12 Aug 2019 20:25:14: 12000000 INFO @ Mon, 12 Aug 2019 20:25:20: 14000000 INFO @ Mon, 12 Aug 2019 20:25:21: 14000000 INFO @ Mon, 12 Aug 2019 20:25:23: 13000000 INFO @ Mon, 12 Aug 2019 20:25:28: 15000000 INFO @ Mon, 12 Aug 2019 20:25:29: 15000000 INFO @ Mon, 12 Aug 2019 20:25:31: 14000000 INFO @ Mon, 12 Aug 2019 20:25:36: 16000000 INFO @ Mon, 12 Aug 2019 20:25:37: 16000000 INFO @ Mon, 12 Aug 2019 20:25:40: 15000000 INFO @ Mon, 12 Aug 2019 20:25:44: 17000000 INFO @ Mon, 12 Aug 2019 20:25:45: 17000000 INFO @ Mon, 12 Aug 2019 20:25:49: 16000000 INFO @ Mon, 12 Aug 2019 20:25:52: 18000000 INFO @ Mon, 12 Aug 2019 20:25:53: 18000000 INFO @ Mon, 12 Aug 2019 20:25:58: 17000000 INFO @ Mon, 12 Aug 2019 20:25:59: 19000000 INFO @ Mon, 12 Aug 2019 20:26:01: 19000000 INFO @ Mon, 12 Aug 2019 20:26:06: 18000000 INFO @ Mon, 12 Aug 2019 20:26:07: 20000000 INFO @ Mon, 12 Aug 2019 20:26:09: 20000000 INFO @ Mon, 12 Aug 2019 20:26:15: 19000000 INFO @ Mon, 12 Aug 2019 20:26:15: 21000000 INFO @ Mon, 12 Aug 2019 20:26:17: 21000000 INFO @ Mon, 12 Aug 2019 20:26:23: 22000000 INFO @ Mon, 12 Aug 2019 20:26:24: 20000000 INFO @ Mon, 12 Aug 2019 20:26:25: 22000000 INFO @ Mon, 12 Aug 2019 20:26:31: 23000000 INFO @ Mon, 12 Aug 2019 20:26:32: 21000000 INFO @ Mon, 12 Aug 2019 20:26:33: 23000000 INFO @ Mon, 12 Aug 2019 20:26:39: 24000000 INFO @ Mon, 12 Aug 2019 20:26:41: 24000000 INFO @ Mon, 12 Aug 2019 20:26:41: 22000000 INFO @ Mon, 12 Aug 2019 20:26:47: 25000000 INFO @ Mon, 12 Aug 2019 20:26:49: 25000000 INFO @ Mon, 12 Aug 2019 20:26:50: 23000000 INFO @ Mon, 12 Aug 2019 20:26:55: 26000000 INFO @ Mon, 12 Aug 2019 20:26:57: 26000000 INFO @ Mon, 12 Aug 2019 20:26:59: 24000000 INFO @ Mon, 12 Aug 2019 20:27:02: 27000000 INFO @ Mon, 12 Aug 2019 20:27:04: 27000000 INFO @ Mon, 12 Aug 2019 20:27:07: 25000000 INFO @ Mon, 12 Aug 2019 20:27:09: 28000000 INFO @ Mon, 12 Aug 2019 20:27:11: 28000000 INFO @ Mon, 12 Aug 2019 20:27:16: 29000000 INFO @ Mon, 12 Aug 2019 20:27:16: 26000000 INFO @ Mon, 12 Aug 2019 20:27:18: 29000000 INFO @ Mon, 12 Aug 2019 20:27:23: 30000000 INFO @ Mon, 12 Aug 2019 20:27:24: 27000000 INFO @ Mon, 12 Aug 2019 20:27:25: 30000000 INFO @ Mon, 12 Aug 2019 20:27:30: 31000000 INFO @ Mon, 12 Aug 2019 20:27:32: 31000000 INFO @ Mon, 12 Aug 2019 20:27:33: 28000000 INFO @ Mon, 12 Aug 2019 20:27:37: 32000000 INFO @ Mon, 12 Aug 2019 20:27:39: 32000000 INFO @ Mon, 12 Aug 2019 20:27:42: 29000000 INFO @ Mon, 12 Aug 2019 20:27:43: 33000000 INFO @ Mon, 12 Aug 2019 20:27:46: 33000000 INFO @ Mon, 12 Aug 2019 20:27:51: 34000000 INFO @ Mon, 12 Aug 2019 20:27:51: 30000000 INFO @ Mon, 12 Aug 2019 20:27:53: 34000000 INFO @ Mon, 12 Aug 2019 20:27:58: 35000000 INFO @ Mon, 12 Aug 2019 20:28:00: 35000000 INFO @ Mon, 12 Aug 2019 20:28:00: 31000000 INFO @ Mon, 12 Aug 2019 20:28:05: 36000000 INFO @ Mon, 12 Aug 2019 20:28:07: 36000000 INFO @ Mon, 12 Aug 2019 20:28:09: 32000000 INFO @ Mon, 12 Aug 2019 20:28:10: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 20:28:10: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 20:28:10: #1 total tags in treatment: 36622314 INFO @ Mon, 12 Aug 2019 20:28:10: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 20:28:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 20:28:11: #1 tags after filtering in treatment: 36622314 INFO @ Mon, 12 Aug 2019 20:28:11: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 20:28:11: #1 finished! INFO @ Mon, 12 Aug 2019 20:28:11: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 20:28:11: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 20:28:12: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 20:28:12: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 20:28:12: #1 total tags in treatment: 36622314 INFO @ Mon, 12 Aug 2019 20:28:12: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 20:28:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 20:28:13: #1 tags after filtering in treatment: 36622314 INFO @ Mon, 12 Aug 2019 20:28:13: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 20:28:13: #1 finished! INFO @ Mon, 12 Aug 2019 20:28:13: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 20:28:13: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 20:28:14: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 20:28:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 20:28:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX495078/SRX495078.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX495078/SRX495078.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX495078/SRX495078.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX495078/SRX495078.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 20:28:16: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 20:28:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 20:28:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX495078/SRX495078.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX495078/SRX495078.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX495078/SRX495078.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX495078/SRX495078.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 20:28:19: 33000000 INFO @ Mon, 12 Aug 2019 20:28:28: 34000000 INFO @ Mon, 12 Aug 2019 20:28:37: 35000000 INFO @ Mon, 12 Aug 2019 20:28:46: 36000000 INFO @ Mon, 12 Aug 2019 20:28:52: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 20:28:52: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 20:28:52: #1 total tags in treatment: 36622314 INFO @ Mon, 12 Aug 2019 20:28:52: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 20:28:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 20:28:53: #1 tags after filtering in treatment: 36622314 INFO @ Mon, 12 Aug 2019 20:28:53: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 20:28:53: #1 finished! INFO @ Mon, 12 Aug 2019 20:28:53: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 20:28:53: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 20:28:56: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 20:28:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 20:28:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX495078/SRX495078.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX495078/SRX495078.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX495078/SRX495078.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX495078/SRX495078.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。