Job ID = 1292804 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-06-02T11:34:53 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T11:34:53 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 13,338,445 reads read : 13,338,445 reads written : 13,338,445 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:55 13338445 reads; of these: 13338445 (100.00%) were unpaired; of these: 89000 (0.67%) aligned 0 times 10992290 (82.41%) aligned exactly 1 time 2257155 (16.92%) aligned >1 times 99.33% overall alignment rate Time searching: 00:02:55 Overall time: 00:02:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 2114233 / 13249445 = 0.1596 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 20:45:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX495050/SRX495050.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX495050/SRX495050.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX495050/SRX495050.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX495050/SRX495050.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 20:45:15: #1 read tag files... INFO @ Sun, 02 Jun 2019 20:45:15: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 20:45:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX495050/SRX495050.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX495050/SRX495050.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX495050/SRX495050.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX495050/SRX495050.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 20:45:15: #1 read tag files... INFO @ Sun, 02 Jun 2019 20:45:15: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 20:45:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX495050/SRX495050.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX495050/SRX495050.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX495050/SRX495050.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX495050/SRX495050.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 20:45:15: #1 read tag files... INFO @ Sun, 02 Jun 2019 20:45:15: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 20:45:23: 1000000 INFO @ Sun, 02 Jun 2019 20:45:23: 1000000 INFO @ Sun, 02 Jun 2019 20:45:24: 1000000 INFO @ Sun, 02 Jun 2019 20:45:30: 2000000 INFO @ Sun, 02 Jun 2019 20:45:30: 2000000 INFO @ Sun, 02 Jun 2019 20:45:31: 2000000 INFO @ Sun, 02 Jun 2019 20:45:38: 3000000 INFO @ Sun, 02 Jun 2019 20:45:38: 3000000 INFO @ Sun, 02 Jun 2019 20:45:39: 3000000 INFO @ Sun, 02 Jun 2019 20:45:45: 4000000 INFO @ Sun, 02 Jun 2019 20:45:45: 4000000 INFO @ Sun, 02 Jun 2019 20:45:47: 4000000 INFO @ Sun, 02 Jun 2019 20:45:53: 5000000 INFO @ Sun, 02 Jun 2019 20:45:53: 5000000 INFO @ Sun, 02 Jun 2019 20:45:55: 5000000 INFO @ Sun, 02 Jun 2019 20:46:00: 6000000 INFO @ Sun, 02 Jun 2019 20:46:00: 6000000 INFO @ Sun, 02 Jun 2019 20:46:03: 6000000 INFO @ Sun, 02 Jun 2019 20:46:07: 7000000 INFO @ Sun, 02 Jun 2019 20:46:08: 7000000 INFO @ Sun, 02 Jun 2019 20:46:11: 7000000 INFO @ Sun, 02 Jun 2019 20:46:15: 8000000 INFO @ Sun, 02 Jun 2019 20:46:15: 8000000 INFO @ Sun, 02 Jun 2019 20:46:19: 8000000 INFO @ Sun, 02 Jun 2019 20:46:22: 9000000 INFO @ Sun, 02 Jun 2019 20:46:22: 9000000 INFO @ Sun, 02 Jun 2019 20:46:27: 9000000 INFO @ Sun, 02 Jun 2019 20:46:29: 10000000 INFO @ Sun, 02 Jun 2019 20:46:30: 10000000 INFO @ Sun, 02 Jun 2019 20:46:35: 10000000 INFO @ Sun, 02 Jun 2019 20:46:37: 11000000 INFO @ Sun, 02 Jun 2019 20:46:37: 11000000 INFO @ Sun, 02 Jun 2019 20:46:38: #1 tag size is determined as 42 bps INFO @ Sun, 02 Jun 2019 20:46:38: #1 tag size = 42 INFO @ Sun, 02 Jun 2019 20:46:38: #1 total tags in treatment: 11135212 INFO @ Sun, 02 Jun 2019 20:46:38: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 20:46:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 20:46:38: #1 tags after filtering in treatment: 11135212 INFO @ Sun, 02 Jun 2019 20:46:38: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 20:46:38: #1 finished! INFO @ Sun, 02 Jun 2019 20:46:38: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 20:46:38: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 20:46:38: #1 tag size is determined as 42 bps INFO @ Sun, 02 Jun 2019 20:46:38: #1 tag size = 42 INFO @ Sun, 02 Jun 2019 20:46:38: #1 total tags in treatment: 11135212 INFO @ Sun, 02 Jun 2019 20:46:38: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 20:46:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 20:46:38: #1 tags after filtering in treatment: 11135212 INFO @ Sun, 02 Jun 2019 20:46:38: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 20:46:38: #1 finished! INFO @ Sun, 02 Jun 2019 20:46:38: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 20:46:38: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 20:46:39: #2 number of paired peaks: 364 WARNING @ Sun, 02 Jun 2019 20:46:39: Fewer paired peaks (364) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 364 pairs to build model! INFO @ Sun, 02 Jun 2019 20:46:39: start model_add_line... INFO @ Sun, 02 Jun 2019 20:46:39: start X-correlation... INFO @ Sun, 02 Jun 2019 20:46:39: end of X-cor INFO @ Sun, 02 Jun 2019 20:46:39: #2 finished! INFO @ Sun, 02 Jun 2019 20:46:39: #2 predicted fragment length is 40 bps INFO @ Sun, 02 Jun 2019 20:46:39: #2 alternative fragment length(s) may be 2,40 bps INFO @ Sun, 02 Jun 2019 20:46:39: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX495050/SRX495050.10_model.r WARNING @ Sun, 02 Jun 2019 20:46:39: #2 Since the d (40) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 20:46:39: #2 You may need to consider one of the other alternative d(s): 2,40 WARNING @ Sun, 02 Jun 2019 20:46:39: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 20:46:39: #3 Call peaks... INFO @ Sun, 02 Jun 2019 20:46:39: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 20:46:39: #2 number of paired peaks: 364 WARNING @ Sun, 02 Jun 2019 20:46:39: Fewer paired peaks (364) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 364 pairs to build model! INFO @ Sun, 02 Jun 2019 20:46:39: start model_add_line... INFO @ Sun, 02 Jun 2019 20:46:39: start X-correlation... INFO @ Sun, 02 Jun 2019 20:46:39: end of X-cor INFO @ Sun, 02 Jun 2019 20:46:39: #2 finished! INFO @ Sun, 02 Jun 2019 20:46:39: #2 predicted fragment length is 40 bps INFO @ Sun, 02 Jun 2019 20:46:39: #2 alternative fragment length(s) may be 2,40 bps INFO @ Sun, 02 Jun 2019 20:46:39: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX495050/SRX495050.20_model.r WARNING @ Sun, 02 Jun 2019 20:46:39: #2 Since the d (40) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 20:46:39: #2 You may need to consider one of the other alternative d(s): 2,40 WARNING @ Sun, 02 Jun 2019 20:46:39: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 20:46:39: #3 Call peaks... INFO @ Sun, 02 Jun 2019 20:46:39: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 20:46:43: 11000000 INFO @ Sun, 02 Jun 2019 20:46:44: #1 tag size is determined as 42 bps INFO @ Sun, 02 Jun 2019 20:46:44: #1 tag size = 42 INFO @ Sun, 02 Jun 2019 20:46:44: #1 total tags in treatment: 11135212 INFO @ Sun, 02 Jun 2019 20:46:44: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 20:46:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 20:46:44: #1 tags after filtering in treatment: 11135212 INFO @ Sun, 02 Jun 2019 20:46:44: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 20:46:44: #1 finished! INFO @ Sun, 02 Jun 2019 20:46:44: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 20:46:44: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 20:46:45: #2 number of paired peaks: 364 WARNING @ Sun, 02 Jun 2019 20:46:45: Fewer paired peaks (364) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 364 pairs to build model! INFO @ Sun, 02 Jun 2019 20:46:45: start model_add_line... INFO @ Sun, 02 Jun 2019 20:46:45: start X-correlation... INFO @ Sun, 02 Jun 2019 20:46:45: end of X-cor INFO @ Sun, 02 Jun 2019 20:46:45: #2 finished! INFO @ Sun, 02 Jun 2019 20:46:45: #2 predicted fragment length is 40 bps INFO @ Sun, 02 Jun 2019 20:46:45: #2 alternative fragment length(s) may be 2,40 bps INFO @ Sun, 02 Jun 2019 20:46:45: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX495050/SRX495050.05_model.r WARNING @ Sun, 02 Jun 2019 20:46:45: #2 Since the d (40) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 20:46:45: #2 You may need to consider one of the other alternative d(s): 2,40 WARNING @ Sun, 02 Jun 2019 20:46:45: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 20:46:45: #3 Call peaks... INFO @ Sun, 02 Jun 2019 20:46:45: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 20:47:08: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 20:47:08: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 20:47:14: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 20:47:23: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX495050/SRX495050.20_peaks.xls INFO @ Sun, 02 Jun 2019 20:47:23: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX495050/SRX495050.20_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 20:47:23: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX495050/SRX495050.20_summits.bed INFO @ Sun, 02 Jun 2019 20:47:23: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (128 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 20:47:23: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX495050/SRX495050.10_peaks.xls INFO @ Sun, 02 Jun 2019 20:47:23: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX495050/SRX495050.10_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 20:47:23: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX495050/SRX495050.10_summits.bed INFO @ Sun, 02 Jun 2019 20:47:23: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (362 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 20:47:29: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX495050/SRX495050.05_peaks.xls INFO @ Sun, 02 Jun 2019 20:47:29: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX495050/SRX495050.05_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 20:47:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX495050/SRX495050.05_summits.bed INFO @ Sun, 02 Jun 2019 20:47:29: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (913 records, 4 fields): 5 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。