Job ID = 4303042 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 47,950,615 reads read : 47,950,615 reads written : 47,950,615 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1198439.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:56 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3139815 (6.55%) aligned 0 times 37484895 (78.17%) aligned exactly 1 time 7325905 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:10:57 Overall time: 00:10:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8188421 / 44810800 = 0.1827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 12 Dec 2019 00:59:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494907/SRX494907.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494907/SRX494907.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494907/SRX494907.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494907/SRX494907.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 00:59:22: #1 read tag files... INFO @ Thu, 12 Dec 2019 00:59:22: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 00:59:27: 1000000 INFO @ Thu, 12 Dec 2019 00:59:33: 2000000 INFO @ Thu, 12 Dec 2019 00:59:38: 3000000 INFO @ Thu, 12 Dec 2019 00:59:44: 4000000 INFO @ Thu, 12 Dec 2019 00:59:49: 5000000 INFO @ Thu, 12 Dec 2019 00:59:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494907/SRX494907.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494907/SRX494907.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494907/SRX494907.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494907/SRX494907.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 00:59:51: #1 read tag files... INFO @ Thu, 12 Dec 2019 00:59:51: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 00:59:55: 6000000 INFO @ Thu, 12 Dec 2019 00:59:57: 1000000 INFO @ Thu, 12 Dec 2019 01:00:01: 7000000 INFO @ Thu, 12 Dec 2019 01:00:03: 2000000 INFO @ Thu, 12 Dec 2019 01:00:06: 8000000 INFO @ Thu, 12 Dec 2019 01:00:09: 3000000 INFO @ Thu, 12 Dec 2019 01:00:12: 9000000 INFO @ Thu, 12 Dec 2019 01:00:15: 4000000 INFO @ Thu, 12 Dec 2019 01:00:18: 10000000 BedGraph に変換中... INFO @ Thu, 12 Dec 2019 01:00:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494907/SRX494907.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494907/SRX494907.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494907/SRX494907.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494907/SRX494907.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 01:00:21: #1 read tag files... INFO @ Thu, 12 Dec 2019 01:00:21: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 01:00:22: 5000000 INFO @ Thu, 12 Dec 2019 01:00:24: 11000000 INFO @ Thu, 12 Dec 2019 01:00:28: 1000000 INFO @ Thu, 12 Dec 2019 01:00:29: 6000000 INFO @ Thu, 12 Dec 2019 01:00:30: 12000000 INFO @ Thu, 12 Dec 2019 01:00:35: 2000000 INFO @ Thu, 12 Dec 2019 01:00:35: 13000000 INFO @ Thu, 12 Dec 2019 01:00:36: 7000000 INFO @ Thu, 12 Dec 2019 01:00:41: 14000000 INFO @ Thu, 12 Dec 2019 01:00:42: 3000000 INFO @ Thu, 12 Dec 2019 01:00:43: 8000000 INFO @ Thu, 12 Dec 2019 01:00:47: 15000000 INFO @ Thu, 12 Dec 2019 01:00:49: 4000000 INFO @ Thu, 12 Dec 2019 01:00:50: 9000000 INFO @ Thu, 12 Dec 2019 01:00:52: 16000000 INFO @ Thu, 12 Dec 2019 01:00:56: 5000000 INFO @ Thu, 12 Dec 2019 01:00:57: 10000000 INFO @ Thu, 12 Dec 2019 01:00:58: 17000000 INFO @ Thu, 12 Dec 2019 01:01:03: 6000000 INFO @ Thu, 12 Dec 2019 01:01:04: 11000000 INFO @ Thu, 12 Dec 2019 01:01:04: 18000000 INFO @ Thu, 12 Dec 2019 01:01:09: 19000000 INFO @ Thu, 12 Dec 2019 01:01:10: 7000000 INFO @ Thu, 12 Dec 2019 01:01:11: 12000000 INFO @ Thu, 12 Dec 2019 01:01:15: 20000000 INFO @ Thu, 12 Dec 2019 01:01:18: 8000000 INFO @ Thu, 12 Dec 2019 01:01:18: 13000000 INFO @ Thu, 12 Dec 2019 01:01:20: 21000000 INFO @ Thu, 12 Dec 2019 01:01:25: 9000000 INFO @ Thu, 12 Dec 2019 01:01:25: 14000000 INFO @ Thu, 12 Dec 2019 01:01:26: 22000000 INFO @ Thu, 12 Dec 2019 01:01:32: 10000000 INFO @ Thu, 12 Dec 2019 01:01:32: 15000000 INFO @ Thu, 12 Dec 2019 01:01:32: 23000000 INFO @ Thu, 12 Dec 2019 01:01:37: 24000000 INFO @ Thu, 12 Dec 2019 01:01:39: 11000000 INFO @ Thu, 12 Dec 2019 01:01:39: 16000000 INFO @ Thu, 12 Dec 2019 01:01:43: 25000000 INFO @ Thu, 12 Dec 2019 01:01:46: 17000000 INFO @ Thu, 12 Dec 2019 01:01:46: 12000000 INFO @ Thu, 12 Dec 2019 01:01:49: 26000000 INFO @ Thu, 12 Dec 2019 01:01:52: 18000000 INFO @ Thu, 12 Dec 2019 01:01:53: 13000000 INFO @ Thu, 12 Dec 2019 01:01:54: 27000000 INFO @ Thu, 12 Dec 2019 01:01:59: 19000000 INFO @ Thu, 12 Dec 2019 01:02:00: 14000000 INFO @ Thu, 12 Dec 2019 01:02:00: 28000000 INFO @ Thu, 12 Dec 2019 01:02:06: 20000000 INFO @ Thu, 12 Dec 2019 01:02:06: 29000000 INFO @ Thu, 12 Dec 2019 01:02:06: 15000000 INFO @ Thu, 12 Dec 2019 01:02:12: 30000000 INFO @ Thu, 12 Dec 2019 01:02:12: 21000000 INFO @ Thu, 12 Dec 2019 01:02:13: 16000000 INFO @ Thu, 12 Dec 2019 01:02:17: 31000000 INFO @ Thu, 12 Dec 2019 01:02:19: 22000000 INFO @ Thu, 12 Dec 2019 01:02:20: 17000000 INFO @ Thu, 12 Dec 2019 01:02:23: 32000000 INFO @ Thu, 12 Dec 2019 01:02:26: 23000000 INFO @ Thu, 12 Dec 2019 01:02:27: 18000000 INFO @ Thu, 12 Dec 2019 01:02:29: 33000000 INFO @ Thu, 12 Dec 2019 01:02:33: 24000000 INFO @ Thu, 12 Dec 2019 01:02:34: 34000000 INFO @ Thu, 12 Dec 2019 01:02:34: 19000000 INFO @ Thu, 12 Dec 2019 01:02:40: 25000000 INFO @ Thu, 12 Dec 2019 01:02:40: 35000000 INFO @ Thu, 12 Dec 2019 01:02:41: 20000000 INFO @ Thu, 12 Dec 2019 01:02:46: 36000000 INFO @ Thu, 12 Dec 2019 01:02:47: 26000000 INFO @ Thu, 12 Dec 2019 01:02:48: 21000000 INFO @ Thu, 12 Dec 2019 01:02:49: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:02:49: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:02:49: #1 total tags in treatment: 36622379 INFO @ Thu, 12 Dec 2019 01:02:49: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:02:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:02:50: #1 tags after filtering in treatment: 36622379 INFO @ Thu, 12 Dec 2019 01:02:50: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:02:50: #1 finished! INFO @ Thu, 12 Dec 2019 01:02:50: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:02:50: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:02:52: #2 number of paired peaks: 0 WARNING @ Thu, 12 Dec 2019 01:02:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:02:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494907/SRX494907.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494907/SRX494907.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494907/SRX494907.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494907/SRX494907.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 01:02:53: 27000000 INFO @ Thu, 12 Dec 2019 01:02:55: 22000000 INFO @ Thu, 12 Dec 2019 01:03:00: 28000000 INFO @ Thu, 12 Dec 2019 01:03:02: 23000000 INFO @ Thu, 12 Dec 2019 01:03:07: 29000000 INFO @ Thu, 12 Dec 2019 01:03:09: 24000000 INFO @ Thu, 12 Dec 2019 01:03:14: 30000000 INFO @ Thu, 12 Dec 2019 01:03:16: 25000000 INFO @ Thu, 12 Dec 2019 01:03:21: 31000000 INFO @ Thu, 12 Dec 2019 01:03:22: 26000000 INFO @ Thu, 12 Dec 2019 01:03:27: 32000000 INFO @ Thu, 12 Dec 2019 01:03:29: 27000000 INFO @ Thu, 12 Dec 2019 01:03:34: 33000000 INFO @ Thu, 12 Dec 2019 01:03:36: 28000000 INFO @ Thu, 12 Dec 2019 01:03:41: 34000000 INFO @ Thu, 12 Dec 2019 01:03:42: 29000000 INFO @ Thu, 12 Dec 2019 01:03:47: 35000000 INFO @ Thu, 12 Dec 2019 01:03:49: 30000000 INFO @ Thu, 12 Dec 2019 01:03:54: 36000000 INFO @ Thu, 12 Dec 2019 01:03:56: 31000000 INFO @ Thu, 12 Dec 2019 01:03:58: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:03:58: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:03:58: #1 total tags in treatment: 36622379 INFO @ Thu, 12 Dec 2019 01:03:58: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:03:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:03:59: #1 tags after filtering in treatment: 36622379 INFO @ Thu, 12 Dec 2019 01:03:59: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:03:59: #1 finished! INFO @ Thu, 12 Dec 2019 01:03:59: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:03:59: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:04:01: #2 number of paired peaks: 0 WARNING @ Thu, 12 Dec 2019 01:04:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:04:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494907/SRX494907.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494907/SRX494907.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494907/SRX494907.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494907/SRX494907.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 01:04:02: 32000000 INFO @ Thu, 12 Dec 2019 01:04:08: 33000000 INFO @ Thu, 12 Dec 2019 01:04:14: 34000000 INFO @ Thu, 12 Dec 2019 01:04:20: 35000000 INFO @ Thu, 12 Dec 2019 01:04:26: 36000000 INFO @ Thu, 12 Dec 2019 01:04:30: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:04:30: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:04:30: #1 total tags in treatment: 36622379 INFO @ Thu, 12 Dec 2019 01:04:30: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:04:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:04:31: #1 tags after filtering in treatment: 36622379 INFO @ Thu, 12 Dec 2019 01:04:31: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:04:31: #1 finished! INFO @ Thu, 12 Dec 2019 01:04:31: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:04:31: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:04:33: #2 number of paired peaks: 0 WARNING @ Thu, 12 Dec 2019 01:04:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:04:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494907/SRX494907.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494907/SRX494907.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494907/SRX494907.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494907/SRX494907.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。