Job ID = 2590051 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 47,950,615 reads read : 47,950,615 reads written : 47,950,615 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1198427.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:12 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3139856 (6.55%) aligned 0 times 37484887 (78.17%) aligned exactly 1 time 7325872 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:13:13 Overall time: 00:13:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8188517 / 44810759 = 0.1827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 19:43:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494895/SRX494895.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494895/SRX494895.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494895/SRX494895.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494895/SRX494895.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 19:43:14: #1 read tag files... INFO @ Mon, 12 Aug 2019 19:43:14: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 19:43:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494895/SRX494895.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494895/SRX494895.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494895/SRX494895.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494895/SRX494895.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 19:43:15: #1 read tag files... INFO @ Mon, 12 Aug 2019 19:43:15: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 19:43:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494895/SRX494895.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494895/SRX494895.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494895/SRX494895.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494895/SRX494895.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 19:43:16: #1 read tag files... INFO @ Mon, 12 Aug 2019 19:43:16: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 19:43:22: 1000000 INFO @ Mon, 12 Aug 2019 19:43:23: 1000000 INFO @ Mon, 12 Aug 2019 19:43:26: 1000000 INFO @ Mon, 12 Aug 2019 19:43:29: 2000000 INFO @ Mon, 12 Aug 2019 19:43:30: 2000000 INFO @ Mon, 12 Aug 2019 19:43:36: 2000000 INFO @ Mon, 12 Aug 2019 19:43:37: 3000000 INFO @ Mon, 12 Aug 2019 19:43:38: 3000000 INFO @ Mon, 12 Aug 2019 19:43:45: 4000000 INFO @ Mon, 12 Aug 2019 19:43:45: 3000000 INFO @ Mon, 12 Aug 2019 19:43:46: 4000000 INFO @ Mon, 12 Aug 2019 19:43:52: 5000000 INFO @ Mon, 12 Aug 2019 19:43:53: 5000000 INFO @ Mon, 12 Aug 2019 19:43:54: 4000000 INFO @ Mon, 12 Aug 2019 19:44:00: 6000000 INFO @ Mon, 12 Aug 2019 19:44:01: 6000000 INFO @ Mon, 12 Aug 2019 19:44:04: 5000000 INFO @ Mon, 12 Aug 2019 19:44:08: 7000000 INFO @ Mon, 12 Aug 2019 19:44:09: 7000000 INFO @ Mon, 12 Aug 2019 19:44:14: 6000000 INFO @ Mon, 12 Aug 2019 19:44:15: 8000000 INFO @ Mon, 12 Aug 2019 19:44:17: 8000000 INFO @ Mon, 12 Aug 2019 19:44:23: 9000000 INFO @ Mon, 12 Aug 2019 19:44:24: 9000000 INFO @ Mon, 12 Aug 2019 19:44:24: 7000000 INFO @ Mon, 12 Aug 2019 19:44:30: 10000000 INFO @ Mon, 12 Aug 2019 19:44:32: 10000000 INFO @ Mon, 12 Aug 2019 19:44:34: 8000000 INFO @ Mon, 12 Aug 2019 19:44:38: 11000000 INFO @ Mon, 12 Aug 2019 19:44:39: 11000000 INFO @ Mon, 12 Aug 2019 19:44:44: 9000000 INFO @ Mon, 12 Aug 2019 19:44:45: 12000000 INFO @ Mon, 12 Aug 2019 19:44:47: 12000000 INFO @ Mon, 12 Aug 2019 19:44:53: 10000000 INFO @ Mon, 12 Aug 2019 19:44:53: 13000000 INFO @ Mon, 12 Aug 2019 19:44:55: 13000000 INFO @ Mon, 12 Aug 2019 19:45:01: 14000000 INFO @ Mon, 12 Aug 2019 19:45:02: 14000000 INFO @ Mon, 12 Aug 2019 19:45:02: 11000000 INFO @ Mon, 12 Aug 2019 19:45:08: 15000000 INFO @ Mon, 12 Aug 2019 19:45:10: 15000000 INFO @ Mon, 12 Aug 2019 19:45:12: 12000000 INFO @ Mon, 12 Aug 2019 19:45:16: 16000000 INFO @ Mon, 12 Aug 2019 19:45:18: 16000000 INFO @ Mon, 12 Aug 2019 19:45:21: 13000000 INFO @ Mon, 12 Aug 2019 19:45:23: 17000000 INFO @ Mon, 12 Aug 2019 19:45:25: 17000000 INFO @ Mon, 12 Aug 2019 19:45:31: 14000000 INFO @ Mon, 12 Aug 2019 19:45:31: 18000000 INFO @ Mon, 12 Aug 2019 19:45:33: 18000000 INFO @ Mon, 12 Aug 2019 19:45:38: 19000000 INFO @ Mon, 12 Aug 2019 19:45:40: 15000000 INFO @ Mon, 12 Aug 2019 19:45:40: 19000000 INFO @ Mon, 12 Aug 2019 19:45:46: 20000000 INFO @ Mon, 12 Aug 2019 19:45:48: 20000000 INFO @ Mon, 12 Aug 2019 19:45:49: 16000000 INFO @ Mon, 12 Aug 2019 19:45:53: 21000000 INFO @ Mon, 12 Aug 2019 19:45:56: 21000000 INFO @ Mon, 12 Aug 2019 19:45:58: 17000000 INFO @ Mon, 12 Aug 2019 19:46:01: 22000000 INFO @ Mon, 12 Aug 2019 19:46:03: 22000000 INFO @ Mon, 12 Aug 2019 19:46:07: 18000000 INFO @ Mon, 12 Aug 2019 19:46:09: 23000000 INFO @ Mon, 12 Aug 2019 19:46:11: 23000000 INFO @ Mon, 12 Aug 2019 19:46:16: 24000000 INFO @ Mon, 12 Aug 2019 19:46:16: 19000000 INFO @ Mon, 12 Aug 2019 19:46:19: 24000000 INFO @ Mon, 12 Aug 2019 19:46:24: 25000000 INFO @ Mon, 12 Aug 2019 19:46:25: 20000000 INFO @ Mon, 12 Aug 2019 19:46:26: 25000000 INFO @ Mon, 12 Aug 2019 19:46:31: 26000000 INFO @ Mon, 12 Aug 2019 19:46:34: 26000000 INFO @ Mon, 12 Aug 2019 19:46:34: 21000000 INFO @ Mon, 12 Aug 2019 19:46:39: 27000000 INFO @ Mon, 12 Aug 2019 19:46:42: 27000000 INFO @ Mon, 12 Aug 2019 19:46:44: 22000000 INFO @ Mon, 12 Aug 2019 19:46:47: 28000000 INFO @ Mon, 12 Aug 2019 19:46:49: 28000000 INFO @ Mon, 12 Aug 2019 19:46:53: 23000000 INFO @ Mon, 12 Aug 2019 19:46:54: 29000000 INFO @ Mon, 12 Aug 2019 19:46:57: 29000000 INFO @ Mon, 12 Aug 2019 19:47:02: 30000000 INFO @ Mon, 12 Aug 2019 19:47:02: 24000000 INFO @ Mon, 12 Aug 2019 19:47:04: 30000000 INFO @ Mon, 12 Aug 2019 19:47:09: 31000000 INFO @ Mon, 12 Aug 2019 19:47:12: 25000000 INFO @ Mon, 12 Aug 2019 19:47:12: 31000000 INFO @ Mon, 12 Aug 2019 19:47:17: 32000000 INFO @ Mon, 12 Aug 2019 19:47:20: 32000000 INFO @ Mon, 12 Aug 2019 19:47:21: 26000000 INFO @ Mon, 12 Aug 2019 19:47:24: 33000000 INFO @ Mon, 12 Aug 2019 19:47:27: 33000000 INFO @ Mon, 12 Aug 2019 19:47:30: 27000000 INFO @ Mon, 12 Aug 2019 19:47:32: 34000000 INFO @ Mon, 12 Aug 2019 19:47:35: 34000000 INFO @ Mon, 12 Aug 2019 19:47:39: 35000000 INFO @ Mon, 12 Aug 2019 19:47:40: 28000000 INFO @ Mon, 12 Aug 2019 19:47:43: 35000000 INFO @ Mon, 12 Aug 2019 19:47:47: 36000000 INFO @ Mon, 12 Aug 2019 19:47:49: 29000000 INFO @ Mon, 12 Aug 2019 19:47:50: 36000000 INFO @ Mon, 12 Aug 2019 19:47:52: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 19:47:52: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 19:47:52: #1 total tags in treatment: 36622242 INFO @ Mon, 12 Aug 2019 19:47:52: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 19:47:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 19:47:53: #1 tags after filtering in treatment: 36622242 INFO @ Mon, 12 Aug 2019 19:47:53: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 19:47:53: #1 finished! INFO @ Mon, 12 Aug 2019 19:47:53: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 19:47:53: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 19:47:55: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 19:47:55: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 19:47:55: #1 total tags in treatment: 36622242 INFO @ Mon, 12 Aug 2019 19:47:55: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 19:47:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 19:47:56: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 19:47:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 19:47:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494895/SRX494895.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494895/SRX494895.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494895/SRX494895.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494895/SRX494895.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 19:47:56: #1 tags after filtering in treatment: 36622242 INFO @ Mon, 12 Aug 2019 19:47:56: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 19:47:56: #1 finished! INFO @ Mon, 12 Aug 2019 19:47:56: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 19:47:56: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 19:47:58: 30000000 INFO @ Mon, 12 Aug 2019 19:47:59: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 19:47:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 19:47:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494895/SRX494895.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494895/SRX494895.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494895/SRX494895.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494895/SRX494895.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 19:48:08: 31000000 INFO @ Mon, 12 Aug 2019 19:48:17: 32000000 INFO @ Mon, 12 Aug 2019 19:48:26: 33000000 INFO @ Mon, 12 Aug 2019 19:48:35: 34000000 INFO @ Mon, 12 Aug 2019 19:48:45: 35000000 INFO @ Mon, 12 Aug 2019 19:48:54: 36000000 INFO @ Mon, 12 Aug 2019 19:49:00: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 19:49:00: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 19:49:00: #1 total tags in treatment: 36622242 INFO @ Mon, 12 Aug 2019 19:49:00: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 19:49:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 19:49:01: #1 tags after filtering in treatment: 36622242 INFO @ Mon, 12 Aug 2019 19:49:01: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 19:49:01: #1 finished! INFO @ Mon, 12 Aug 2019 19:49:01: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 19:49:01: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 19:49:04: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 19:49:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 19:49:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494895/SRX494895.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494895/SRX494895.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494895/SRX494895.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494895/SRX494895.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。