Job ID = 2590046 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 47,950,615 reads read : 47,950,615 reads written : 47,950,615 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1198422.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:02 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3139803 (6.55%) aligned 0 times 37484951 (78.17%) aligned exactly 1 time 7325861 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:12:02 Overall time: 00:12:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8188367 / 44810812 = 0.1827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 19:39:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494890/SRX494890.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494890/SRX494890.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494890/SRX494890.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494890/SRX494890.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 19:39:44: #1 read tag files... INFO @ Mon, 12 Aug 2019 19:39:44: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 19:39:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494890/SRX494890.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494890/SRX494890.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494890/SRX494890.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494890/SRX494890.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 19:39:45: #1 read tag files... INFO @ Mon, 12 Aug 2019 19:39:45: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 19:39:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494890/SRX494890.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494890/SRX494890.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494890/SRX494890.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494890/SRX494890.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 19:39:46: #1 read tag files... INFO @ Mon, 12 Aug 2019 19:39:46: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 19:39:54: 1000000 INFO @ Mon, 12 Aug 2019 19:39:54: 1000000 INFO @ Mon, 12 Aug 2019 19:39:55: 1000000 INFO @ Mon, 12 Aug 2019 19:40:01: 2000000 INFO @ Mon, 12 Aug 2019 19:40:03: 2000000 INFO @ Mon, 12 Aug 2019 19:40:04: 2000000 INFO @ Mon, 12 Aug 2019 19:40:09: 3000000 INFO @ Mon, 12 Aug 2019 19:40:13: 3000000 INFO @ Mon, 12 Aug 2019 19:40:13: 3000000 INFO @ Mon, 12 Aug 2019 19:40:16: 4000000 INFO @ Mon, 12 Aug 2019 19:40:22: 4000000 INFO @ Mon, 12 Aug 2019 19:40:23: 4000000 INFO @ Mon, 12 Aug 2019 19:40:23: 5000000 INFO @ Mon, 12 Aug 2019 19:40:31: 6000000 INFO @ Mon, 12 Aug 2019 19:40:31: 5000000 INFO @ Mon, 12 Aug 2019 19:40:32: 5000000 INFO @ Mon, 12 Aug 2019 19:40:38: 7000000 INFO @ Mon, 12 Aug 2019 19:40:40: 6000000 INFO @ Mon, 12 Aug 2019 19:40:41: 6000000 INFO @ Mon, 12 Aug 2019 19:40:45: 8000000 INFO @ Mon, 12 Aug 2019 19:40:49: 7000000 INFO @ Mon, 12 Aug 2019 19:40:51: 7000000 INFO @ Mon, 12 Aug 2019 19:40:53: 9000000 INFO @ Mon, 12 Aug 2019 19:40:58: 8000000 INFO @ Mon, 12 Aug 2019 19:41:00: 10000000 INFO @ Mon, 12 Aug 2019 19:41:00: 8000000 INFO @ Mon, 12 Aug 2019 19:41:07: 11000000 INFO @ Mon, 12 Aug 2019 19:41:07: 9000000 INFO @ Mon, 12 Aug 2019 19:41:10: 9000000 INFO @ Mon, 12 Aug 2019 19:41:15: 12000000 INFO @ Mon, 12 Aug 2019 19:41:16: 10000000 INFO @ Mon, 12 Aug 2019 19:41:19: 10000000 INFO @ Mon, 12 Aug 2019 19:41:22: 13000000 INFO @ Mon, 12 Aug 2019 19:41:25: 11000000 INFO @ Mon, 12 Aug 2019 19:41:28: 11000000 INFO @ Mon, 12 Aug 2019 19:41:29: 14000000 INFO @ Mon, 12 Aug 2019 19:41:34: 12000000 INFO @ Mon, 12 Aug 2019 19:41:37: 15000000 INFO @ Mon, 12 Aug 2019 19:41:38: 12000000 INFO @ Mon, 12 Aug 2019 19:41:43: 13000000 INFO @ Mon, 12 Aug 2019 19:41:44: 16000000 INFO @ Mon, 12 Aug 2019 19:41:47: 13000000 INFO @ Mon, 12 Aug 2019 19:41:52: 17000000 INFO @ Mon, 12 Aug 2019 19:41:52: 14000000 INFO @ Mon, 12 Aug 2019 19:41:57: 14000000 INFO @ Mon, 12 Aug 2019 19:41:59: 18000000 INFO @ Mon, 12 Aug 2019 19:42:01: 15000000 INFO @ Mon, 12 Aug 2019 19:42:06: 15000000 INFO @ Mon, 12 Aug 2019 19:42:06: 19000000 INFO @ Mon, 12 Aug 2019 19:42:10: 16000000 INFO @ Mon, 12 Aug 2019 19:42:13: 20000000 INFO @ Mon, 12 Aug 2019 19:42:15: 16000000 INFO @ Mon, 12 Aug 2019 19:42:19: 17000000 INFO @ Mon, 12 Aug 2019 19:42:21: 21000000 INFO @ Mon, 12 Aug 2019 19:42:25: 17000000 INFO @ Mon, 12 Aug 2019 19:42:28: 22000000 INFO @ Mon, 12 Aug 2019 19:42:28: 18000000 INFO @ Mon, 12 Aug 2019 19:42:34: 18000000 INFO @ Mon, 12 Aug 2019 19:42:35: 23000000 INFO @ Mon, 12 Aug 2019 19:42:37: 19000000 INFO @ Mon, 12 Aug 2019 19:42:42: 24000000 INFO @ Mon, 12 Aug 2019 19:42:43: 19000000 INFO @ Mon, 12 Aug 2019 19:42:46: 20000000 INFO @ Mon, 12 Aug 2019 19:42:50: 25000000 INFO @ Mon, 12 Aug 2019 19:42:52: 20000000 INFO @ Mon, 12 Aug 2019 19:42:55: 21000000 INFO @ Mon, 12 Aug 2019 19:42:57: 26000000 INFO @ Mon, 12 Aug 2019 19:43:02: 21000000 INFO @ Mon, 12 Aug 2019 19:43:04: 22000000 INFO @ Mon, 12 Aug 2019 19:43:04: 27000000 INFO @ Mon, 12 Aug 2019 19:43:11: 22000000 INFO @ Mon, 12 Aug 2019 19:43:11: 28000000 INFO @ Mon, 12 Aug 2019 19:43:13: 23000000 INFO @ Mon, 12 Aug 2019 19:43:18: 29000000 INFO @ Mon, 12 Aug 2019 19:43:20: 23000000 INFO @ Mon, 12 Aug 2019 19:43:22: 24000000 INFO @ Mon, 12 Aug 2019 19:43:25: 30000000 INFO @ Mon, 12 Aug 2019 19:43:30: 24000000 INFO @ Mon, 12 Aug 2019 19:43:31: 25000000 INFO @ Mon, 12 Aug 2019 19:43:33: 31000000 INFO @ Mon, 12 Aug 2019 19:43:39: 25000000 INFO @ Mon, 12 Aug 2019 19:43:40: 26000000 INFO @ Mon, 12 Aug 2019 19:43:40: 32000000 INFO @ Mon, 12 Aug 2019 19:43:47: 33000000 INFO @ Mon, 12 Aug 2019 19:43:48: 26000000 INFO @ Mon, 12 Aug 2019 19:43:48: 27000000 INFO @ Mon, 12 Aug 2019 19:43:54: 34000000 INFO @ Mon, 12 Aug 2019 19:43:57: 28000000 INFO @ Mon, 12 Aug 2019 19:43:57: 27000000 INFO @ Mon, 12 Aug 2019 19:44:01: 35000000 INFO @ Mon, 12 Aug 2019 19:44:06: 29000000 INFO @ Mon, 12 Aug 2019 19:44:06: 28000000 INFO @ Mon, 12 Aug 2019 19:44:08: 36000000 INFO @ Mon, 12 Aug 2019 19:44:13: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 19:44:13: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 19:44:13: #1 total tags in treatment: 36622445 INFO @ Mon, 12 Aug 2019 19:44:13: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 19:44:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 19:44:14: #1 tags after filtering in treatment: 36622445 INFO @ Mon, 12 Aug 2019 19:44:14: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 19:44:14: #1 finished! INFO @ Mon, 12 Aug 2019 19:44:14: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 19:44:14: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 19:44:15: 30000000 INFO @ Mon, 12 Aug 2019 19:44:15: 29000000 INFO @ Mon, 12 Aug 2019 19:44:17: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 19:44:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 19:44:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494890/SRX494890.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494890/SRX494890.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494890/SRX494890.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494890/SRX494890.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 19:44:23: 31000000 INFO @ Mon, 12 Aug 2019 19:44:25: 30000000 INFO @ Mon, 12 Aug 2019 19:44:32: 32000000 INFO @ Mon, 12 Aug 2019 19:44:34: 31000000 INFO @ Mon, 12 Aug 2019 19:44:41: 33000000 INFO @ Mon, 12 Aug 2019 19:44:43: 32000000 INFO @ Mon, 12 Aug 2019 19:44:50: 34000000 INFO @ Mon, 12 Aug 2019 19:44:52: 33000000 INFO @ Mon, 12 Aug 2019 19:44:58: 35000000 INFO @ Mon, 12 Aug 2019 19:45:01: 34000000 INFO @ Mon, 12 Aug 2019 19:45:07: 36000000 INFO @ Mon, 12 Aug 2019 19:45:10: 35000000 INFO @ Mon, 12 Aug 2019 19:45:13: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 19:45:13: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 19:45:13: #1 total tags in treatment: 36622445 INFO @ Mon, 12 Aug 2019 19:45:13: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 19:45:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 19:45:13: #1 tags after filtering in treatment: 36622445 INFO @ Mon, 12 Aug 2019 19:45:13: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 19:45:13: #1 finished! INFO @ Mon, 12 Aug 2019 19:45:13: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 19:45:13: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 19:45:16: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 19:45:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 19:45:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494890/SRX494890.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494890/SRX494890.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494890/SRX494890.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494890/SRX494890.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 19:45:18: 36000000 INFO @ Mon, 12 Aug 2019 19:45:24: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 19:45:24: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 19:45:24: #1 total tags in treatment: 36622445 INFO @ Mon, 12 Aug 2019 19:45:24: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 19:45:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 19:45:25: #1 tags after filtering in treatment: 36622445 INFO @ Mon, 12 Aug 2019 19:45:25: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 19:45:25: #1 finished! INFO @ Mon, 12 Aug 2019 19:45:25: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 19:45:25: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 19:45:28: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 19:45:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 19:45:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494890/SRX494890.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494890/SRX494890.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494890/SRX494890.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494890/SRX494890.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。