Job ID = 2590039 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 47,950,615 reads read : 47,950,615 reads written : 47,950,615 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:02 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3139850 (6.55%) aligned 0 times 37485125 (78.17%) aligned exactly 1 time 7325640 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:12:02 Overall time: 00:12:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8188242 / 44810765 = 0.1827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 20:01:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494883/SRX494883.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494883/SRX494883.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494883/SRX494883.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494883/SRX494883.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 20:01:20: #1 read tag files... INFO @ Mon, 12 Aug 2019 20:01:20: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 20:01:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494883/SRX494883.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494883/SRX494883.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494883/SRX494883.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494883/SRX494883.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 20:01:21: #1 read tag files... INFO @ Mon, 12 Aug 2019 20:01:21: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 20:01:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494883/SRX494883.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494883/SRX494883.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494883/SRX494883.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494883/SRX494883.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 20:01:22: #1 read tag files... INFO @ Mon, 12 Aug 2019 20:01:22: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 20:01:29: 1000000 INFO @ Mon, 12 Aug 2019 20:01:31: 1000000 INFO @ Mon, 12 Aug 2019 20:01:32: 1000000 INFO @ Mon, 12 Aug 2019 20:01:37: 2000000 INFO @ Mon, 12 Aug 2019 20:01:41: 2000000 INFO @ Mon, 12 Aug 2019 20:01:41: 2000000 INFO @ Mon, 12 Aug 2019 20:01:44: 3000000 INFO @ Mon, 12 Aug 2019 20:01:50: 3000000 INFO @ Mon, 12 Aug 2019 20:01:50: 3000000 INFO @ Mon, 12 Aug 2019 20:01:51: 4000000 INFO @ Mon, 12 Aug 2019 20:01:58: 5000000 INFO @ Mon, 12 Aug 2019 20:01:59: 4000000 INFO @ Mon, 12 Aug 2019 20:01:59: 4000000 INFO @ Mon, 12 Aug 2019 20:02:05: 6000000 INFO @ Mon, 12 Aug 2019 20:02:08: 5000000 INFO @ Mon, 12 Aug 2019 20:02:08: 5000000 INFO @ Mon, 12 Aug 2019 20:02:12: 7000000 INFO @ Mon, 12 Aug 2019 20:02:17: 6000000 INFO @ Mon, 12 Aug 2019 20:02:17: 6000000 INFO @ Mon, 12 Aug 2019 20:02:19: 8000000 INFO @ Mon, 12 Aug 2019 20:02:27: 7000000 INFO @ Mon, 12 Aug 2019 20:02:27: 9000000 INFO @ Mon, 12 Aug 2019 20:02:27: 7000000 INFO @ Mon, 12 Aug 2019 20:02:34: 10000000 INFO @ Mon, 12 Aug 2019 20:02:36: 8000000 INFO @ Mon, 12 Aug 2019 20:02:36: 8000000 INFO @ Mon, 12 Aug 2019 20:02:41: 11000000 INFO @ Mon, 12 Aug 2019 20:02:46: 9000000 INFO @ Mon, 12 Aug 2019 20:02:46: 9000000 INFO @ Mon, 12 Aug 2019 20:02:48: 12000000 INFO @ Mon, 12 Aug 2019 20:02:56: 10000000 INFO @ Mon, 12 Aug 2019 20:02:56: 10000000 INFO @ Mon, 12 Aug 2019 20:02:56: 13000000 INFO @ Mon, 12 Aug 2019 20:03:04: 14000000 INFO @ Mon, 12 Aug 2019 20:03:05: 11000000 INFO @ Mon, 12 Aug 2019 20:03:06: 11000000 INFO @ Mon, 12 Aug 2019 20:03:12: 15000000 INFO @ Mon, 12 Aug 2019 20:03:15: 12000000 INFO @ Mon, 12 Aug 2019 20:03:15: 12000000 INFO @ Mon, 12 Aug 2019 20:03:20: 16000000 INFO @ Mon, 12 Aug 2019 20:03:24: 13000000 INFO @ Mon, 12 Aug 2019 20:03:25: 13000000 INFO @ Mon, 12 Aug 2019 20:03:28: 17000000 INFO @ Mon, 12 Aug 2019 20:03:33: 14000000 INFO @ Mon, 12 Aug 2019 20:03:34: 14000000 INFO @ Mon, 12 Aug 2019 20:03:36: 18000000 INFO @ Mon, 12 Aug 2019 20:03:42: 15000000 INFO @ Mon, 12 Aug 2019 20:03:43: 15000000 INFO @ Mon, 12 Aug 2019 20:03:44: 19000000 INFO @ Mon, 12 Aug 2019 20:03:51: 20000000 INFO @ Mon, 12 Aug 2019 20:03:52: 16000000 INFO @ Mon, 12 Aug 2019 20:03:53: 16000000 INFO @ Mon, 12 Aug 2019 20:03:59: 21000000 INFO @ Mon, 12 Aug 2019 20:04:01: 17000000 INFO @ Mon, 12 Aug 2019 20:04:02: 17000000 INFO @ Mon, 12 Aug 2019 20:04:07: 22000000 INFO @ Mon, 12 Aug 2019 20:04:10: 18000000 INFO @ Mon, 12 Aug 2019 20:04:11: 18000000 INFO @ Mon, 12 Aug 2019 20:04:15: 23000000 INFO @ Mon, 12 Aug 2019 20:04:19: 19000000 INFO @ Mon, 12 Aug 2019 20:04:20: 19000000 INFO @ Mon, 12 Aug 2019 20:04:23: 24000000 INFO @ Mon, 12 Aug 2019 20:04:29: 20000000 INFO @ Mon, 12 Aug 2019 20:04:29: 20000000 INFO @ Mon, 12 Aug 2019 20:04:30: 25000000 INFO @ Mon, 12 Aug 2019 20:04:38: 21000000 INFO @ Mon, 12 Aug 2019 20:04:38: 21000000 INFO @ Mon, 12 Aug 2019 20:04:38: 26000000 INFO @ Mon, 12 Aug 2019 20:04:46: 27000000 INFO @ Mon, 12 Aug 2019 20:04:47: 22000000 INFO @ Mon, 12 Aug 2019 20:04:47: 22000000 INFO @ Mon, 12 Aug 2019 20:04:54: 28000000 INFO @ Mon, 12 Aug 2019 20:04:56: 23000000 INFO @ Mon, 12 Aug 2019 20:04:56: 23000000 INFO @ Mon, 12 Aug 2019 20:05:02: 29000000 INFO @ Mon, 12 Aug 2019 20:05:05: 24000000 INFO @ Mon, 12 Aug 2019 20:05:06: 24000000 INFO @ Mon, 12 Aug 2019 20:05:09: 30000000 INFO @ Mon, 12 Aug 2019 20:05:14: 25000000 INFO @ Mon, 12 Aug 2019 20:05:15: 25000000 INFO @ Mon, 12 Aug 2019 20:05:17: 31000000 INFO @ Mon, 12 Aug 2019 20:05:23: 26000000 INFO @ Mon, 12 Aug 2019 20:05:24: 26000000 INFO @ Mon, 12 Aug 2019 20:05:25: 32000000 INFO @ Mon, 12 Aug 2019 20:05:31: 27000000 INFO @ Mon, 12 Aug 2019 20:05:33: 33000000 INFO @ Mon, 12 Aug 2019 20:05:33: 27000000 INFO @ Mon, 12 Aug 2019 20:05:40: 28000000 INFO @ Mon, 12 Aug 2019 20:05:41: 34000000 INFO @ Mon, 12 Aug 2019 20:05:42: 28000000 INFO @ Mon, 12 Aug 2019 20:05:49: 35000000 INFO @ Mon, 12 Aug 2019 20:05:49: 29000000 INFO @ Mon, 12 Aug 2019 20:05:51: 29000000 INFO @ Mon, 12 Aug 2019 20:05:56: 36000000 INFO @ Mon, 12 Aug 2019 20:05:58: 30000000 INFO @ Mon, 12 Aug 2019 20:06:01: 30000000 INFO @ Mon, 12 Aug 2019 20:06:02: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 20:06:02: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 20:06:02: #1 total tags in treatment: 36622523 INFO @ Mon, 12 Aug 2019 20:06:02: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 20:06:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 20:06:02: #1 tags after filtering in treatment: 36622523 INFO @ Mon, 12 Aug 2019 20:06:02: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 20:06:02: #1 finished! INFO @ Mon, 12 Aug 2019 20:06:02: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 20:06:02: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 20:06:05: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 20:06:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 20:06:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494883/SRX494883.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494883/SRX494883.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494883/SRX494883.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494883/SRX494883.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 20:06:07: 31000000 INFO @ Mon, 12 Aug 2019 20:06:10: 31000000 INFO @ Mon, 12 Aug 2019 20:06:16: 32000000 INFO @ Mon, 12 Aug 2019 20:06:19: 32000000 INFO @ Mon, 12 Aug 2019 20:06:24: 33000000 INFO @ Mon, 12 Aug 2019 20:06:28: 33000000 INFO @ Mon, 12 Aug 2019 20:06:33: 34000000 INFO @ Mon, 12 Aug 2019 20:06:37: 34000000 INFO @ Mon, 12 Aug 2019 20:06:42: 35000000 INFO @ Mon, 12 Aug 2019 20:06:46: 35000000 INFO @ Mon, 12 Aug 2019 20:06:50: 36000000 INFO @ Mon, 12 Aug 2019 20:06:55: 36000000 INFO @ Mon, 12 Aug 2019 20:06:56: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 20:06:56: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 20:06:56: #1 total tags in treatment: 36622523 INFO @ Mon, 12 Aug 2019 20:06:56: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 20:06:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 20:06:57: #1 tags after filtering in treatment: 36622523 INFO @ Mon, 12 Aug 2019 20:06:57: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 20:06:57: #1 finished! INFO @ Mon, 12 Aug 2019 20:06:57: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 20:06:57: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 20:07:00: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 20:07:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 20:07:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494883/SRX494883.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494883/SRX494883.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494883/SRX494883.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494883/SRX494883.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 20:07:00: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 20:07:00: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 20:07:00: #1 total tags in treatment: 36622523 INFO @ Mon, 12 Aug 2019 20:07:00: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 20:07:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 20:07:01: #1 tags after filtering in treatment: 36622523 INFO @ Mon, 12 Aug 2019 20:07:01: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 20:07:01: #1 finished! INFO @ Mon, 12 Aug 2019 20:07:01: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 20:07:01: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 20:07:04: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 20:07:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 20:07:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494883/SRX494883.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494883/SRX494883.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494883/SRX494883.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494883/SRX494883.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。