Job ID = 2590020 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 69,236,731 reads read : 69,236,731 reads written : 69,236,731 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:27 69236731 reads; of these: 69236731 (100.00%) were unpaired; of these: 3485722 (5.03%) aligned 0 times 54815709 (79.17%) aligned exactly 1 time 10935300 (15.79%) aligned >1 times 94.97% overall alignment rate Time searching: 00:16:27 Overall time: 00:16:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 28146721 / 65751009 = 0.4281 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 20:14:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494864/SRX494864.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494864/SRX494864.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494864/SRX494864.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494864/SRX494864.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 20:14:28: #1 read tag files... INFO @ Mon, 12 Aug 2019 20:14:28: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 20:14:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494864/SRX494864.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494864/SRX494864.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494864/SRX494864.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494864/SRX494864.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 20:14:29: #1 read tag files... INFO @ Mon, 12 Aug 2019 20:14:29: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 20:14:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494864/SRX494864.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494864/SRX494864.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494864/SRX494864.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494864/SRX494864.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 20:14:30: #1 read tag files... INFO @ Mon, 12 Aug 2019 20:14:30: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 20:14:36: 1000000 INFO @ Mon, 12 Aug 2019 20:14:36: 1000000 INFO @ Mon, 12 Aug 2019 20:14:37: 1000000 INFO @ Mon, 12 Aug 2019 20:14:42: 2000000 INFO @ Mon, 12 Aug 2019 20:14:44: 2000000 INFO @ Mon, 12 Aug 2019 20:14:44: 2000000 INFO @ Mon, 12 Aug 2019 20:14:49: 3000000 INFO @ Mon, 12 Aug 2019 20:14:51: 3000000 INFO @ Mon, 12 Aug 2019 20:14:52: 3000000 INFO @ Mon, 12 Aug 2019 20:14:55: 4000000 INFO @ Mon, 12 Aug 2019 20:14:58: 4000000 INFO @ Mon, 12 Aug 2019 20:14:59: 4000000 INFO @ Mon, 12 Aug 2019 20:15:02: 5000000 INFO @ Mon, 12 Aug 2019 20:15:05: 5000000 INFO @ Mon, 12 Aug 2019 20:15:07: 5000000 INFO @ Mon, 12 Aug 2019 20:15:08: 6000000 INFO @ Mon, 12 Aug 2019 20:15:12: 6000000 INFO @ Mon, 12 Aug 2019 20:15:14: 7000000 INFO @ Mon, 12 Aug 2019 20:15:15: 6000000 INFO @ Mon, 12 Aug 2019 20:15:19: 7000000 INFO @ Mon, 12 Aug 2019 20:15:21: 8000000 INFO @ Mon, 12 Aug 2019 20:15:23: 7000000 INFO @ Mon, 12 Aug 2019 20:15:26: 8000000 INFO @ Mon, 12 Aug 2019 20:15:27: 9000000 INFO @ Mon, 12 Aug 2019 20:15:31: 8000000 INFO @ Mon, 12 Aug 2019 20:15:33: 9000000 INFO @ Mon, 12 Aug 2019 20:15:34: 10000000 INFO @ Mon, 12 Aug 2019 20:15:39: 9000000 INFO @ Mon, 12 Aug 2019 20:15:40: 10000000 INFO @ Mon, 12 Aug 2019 20:15:40: 11000000 INFO @ Mon, 12 Aug 2019 20:15:46: 12000000 INFO @ Mon, 12 Aug 2019 20:15:46: 10000000 INFO @ Mon, 12 Aug 2019 20:15:46: 11000000 INFO @ Mon, 12 Aug 2019 20:15:53: 13000000 INFO @ Mon, 12 Aug 2019 20:15:53: 12000000 INFO @ Mon, 12 Aug 2019 20:15:54: 11000000 INFO @ Mon, 12 Aug 2019 20:15:59: 14000000 INFO @ Mon, 12 Aug 2019 20:16:00: 13000000 INFO @ Mon, 12 Aug 2019 20:16:02: 12000000 INFO @ Mon, 12 Aug 2019 20:16:06: 15000000 INFO @ Mon, 12 Aug 2019 20:16:07: 14000000 INFO @ Mon, 12 Aug 2019 20:16:10: 13000000 INFO @ Mon, 12 Aug 2019 20:16:12: 16000000 INFO @ Mon, 12 Aug 2019 20:16:14: 15000000 INFO @ Mon, 12 Aug 2019 20:16:18: 14000000 INFO @ Mon, 12 Aug 2019 20:16:18: 17000000 INFO @ Mon, 12 Aug 2019 20:16:21: 16000000 INFO @ Mon, 12 Aug 2019 20:16:25: 18000000 INFO @ Mon, 12 Aug 2019 20:16:25: 15000000 INFO @ Mon, 12 Aug 2019 20:16:28: 17000000 INFO @ Mon, 12 Aug 2019 20:16:31: 19000000 INFO @ Mon, 12 Aug 2019 20:16:33: 16000000 INFO @ Mon, 12 Aug 2019 20:16:35: 18000000 INFO @ Mon, 12 Aug 2019 20:16:38: 20000000 INFO @ Mon, 12 Aug 2019 20:16:41: 17000000 INFO @ Mon, 12 Aug 2019 20:16:41: 19000000 INFO @ Mon, 12 Aug 2019 20:16:44: 21000000 INFO @ Mon, 12 Aug 2019 20:16:48: 20000000 INFO @ Mon, 12 Aug 2019 20:16:49: 18000000 INFO @ Mon, 12 Aug 2019 20:16:50: 22000000 INFO @ Mon, 12 Aug 2019 20:16:55: 21000000 INFO @ Mon, 12 Aug 2019 20:16:57: 19000000 INFO @ Mon, 12 Aug 2019 20:16:57: 23000000 INFO @ Mon, 12 Aug 2019 20:17:02: 22000000 INFO @ Mon, 12 Aug 2019 20:17:03: 24000000 INFO @ Mon, 12 Aug 2019 20:17:04: 20000000 INFO @ Mon, 12 Aug 2019 20:17:09: 23000000 INFO @ Mon, 12 Aug 2019 20:17:10: 25000000 INFO @ Mon, 12 Aug 2019 20:17:12: 21000000 INFO @ Mon, 12 Aug 2019 20:17:16: 24000000 INFO @ Mon, 12 Aug 2019 20:17:16: 26000000 INFO @ Mon, 12 Aug 2019 20:17:20: 22000000 INFO @ Mon, 12 Aug 2019 20:17:22: 27000000 INFO @ Mon, 12 Aug 2019 20:17:23: 25000000 INFO @ Mon, 12 Aug 2019 20:17:28: 23000000 INFO @ Mon, 12 Aug 2019 20:17:29: 28000000 INFO @ Mon, 12 Aug 2019 20:17:29: 26000000 INFO @ Mon, 12 Aug 2019 20:17:35: 29000000 INFO @ Mon, 12 Aug 2019 20:17:35: 24000000 INFO @ Mon, 12 Aug 2019 20:17:36: 27000000 INFO @ Mon, 12 Aug 2019 20:17:41: 30000000 INFO @ Mon, 12 Aug 2019 20:17:43: 28000000 INFO @ Mon, 12 Aug 2019 20:17:43: 25000000 INFO @ Mon, 12 Aug 2019 20:17:48: 31000000 INFO @ Mon, 12 Aug 2019 20:17:50: 29000000 INFO @ Mon, 12 Aug 2019 20:17:51: 26000000 INFO @ Mon, 12 Aug 2019 20:17:54: 32000000 INFO @ Mon, 12 Aug 2019 20:17:57: 30000000 INFO @ Mon, 12 Aug 2019 20:17:59: 27000000 INFO @ Mon, 12 Aug 2019 20:18:01: 33000000 INFO @ Mon, 12 Aug 2019 20:18:04: 31000000 INFO @ Mon, 12 Aug 2019 20:18:06: 28000000 INFO @ Mon, 12 Aug 2019 20:18:07: 34000000 INFO @ Mon, 12 Aug 2019 20:18:10: 32000000 INFO @ Mon, 12 Aug 2019 20:18:13: 35000000 INFO @ Mon, 12 Aug 2019 20:18:14: 29000000 INFO @ Mon, 12 Aug 2019 20:18:17: 33000000 INFO @ Mon, 12 Aug 2019 20:18:20: 36000000 INFO @ Mon, 12 Aug 2019 20:18:22: 30000000 INFO @ Mon, 12 Aug 2019 20:18:24: 34000000 INFO @ Mon, 12 Aug 2019 20:18:26: 37000000 INFO @ Mon, 12 Aug 2019 20:18:30: 31000000 INFO @ Mon, 12 Aug 2019 20:18:30: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 20:18:30: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 20:18:30: #1 total tags in treatment: 37604288 INFO @ Mon, 12 Aug 2019 20:18:30: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 20:18:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 20:18:31: 35000000 INFO @ Mon, 12 Aug 2019 20:18:31: #1 tags after filtering in treatment: 37604288 INFO @ Mon, 12 Aug 2019 20:18:31: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 20:18:31: #1 finished! INFO @ Mon, 12 Aug 2019 20:18:31: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 20:18:31: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 20:18:34: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 20:18:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 20:18:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494864/SRX494864.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494864/SRX494864.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494864/SRX494864.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494864/SRX494864.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 20:18:37: 32000000 INFO @ Mon, 12 Aug 2019 20:18:38: 36000000 INFO @ Mon, 12 Aug 2019 20:18:44: 37000000 INFO @ Mon, 12 Aug 2019 20:18:45: 33000000 INFO @ Mon, 12 Aug 2019 20:18:49: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 20:18:49: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 20:18:49: #1 total tags in treatment: 37604288 INFO @ Mon, 12 Aug 2019 20:18:49: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 20:18:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 20:18:50: #1 tags after filtering in treatment: 37604288 INFO @ Mon, 12 Aug 2019 20:18:50: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 20:18:50: #1 finished! INFO @ Mon, 12 Aug 2019 20:18:50: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 20:18:50: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 20:18:53: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 20:18:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 20:18:53: Process for pairing-model is terminated! INFO @ Mon, 12 Aug 2019 20:18:53: 34000000 cut: /home/okishinya/chipatlas/results/ce10/SRX494864/SRX494864.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494864/SRX494864.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494864/SRX494864.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494864/SRX494864.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 20:19:01: 35000000 INFO @ Mon, 12 Aug 2019 20:19:08: 36000000 INFO @ Mon, 12 Aug 2019 20:19:16: 37000000 INFO @ Mon, 12 Aug 2019 20:19:21: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 20:19:21: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 20:19:21: #1 total tags in treatment: 37604288 INFO @ Mon, 12 Aug 2019 20:19:21: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 20:19:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 20:19:22: #1 tags after filtering in treatment: 37604288 INFO @ Mon, 12 Aug 2019 20:19:22: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 20:19:22: #1 finished! INFO @ Mon, 12 Aug 2019 20:19:22: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 20:19:22: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 20:19:25: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 20:19:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 20:19:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494864/SRX494864.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494864/SRX494864.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494864/SRX494864.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494864/SRX494864.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。