Job ID = 2590018 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 47,950,615 reads read : 47,950,615 reads written : 47,950,615 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1198394.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:07 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3139855 (6.55%) aligned 0 times 37485201 (78.17%) aligned exactly 1 time 7325559 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:12:07 Overall time: 00:12:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8189166 / 44810760 = 0.1827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 19:33:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494862/SRX494862.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494862/SRX494862.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494862/SRX494862.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494862/SRX494862.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 19:33:15: #1 read tag files... INFO @ Mon, 12 Aug 2019 19:33:15: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 19:33:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494862/SRX494862.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494862/SRX494862.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494862/SRX494862.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494862/SRX494862.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 19:33:16: #1 read tag files... INFO @ Mon, 12 Aug 2019 19:33:16: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 19:33:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494862/SRX494862.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494862/SRX494862.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494862/SRX494862.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494862/SRX494862.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 19:33:17: #1 read tag files... INFO @ Mon, 12 Aug 2019 19:33:17: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 19:33:23: 1000000 INFO @ Mon, 12 Aug 2019 19:33:25: 1000000 INFO @ Mon, 12 Aug 2019 19:33:26: 1000000 INFO @ Mon, 12 Aug 2019 19:33:33: 2000000 INFO @ Mon, 12 Aug 2019 19:33:34: 2000000 INFO @ Mon, 12 Aug 2019 19:33:34: 2000000 INFO @ Mon, 12 Aug 2019 19:33:42: 3000000 INFO @ Mon, 12 Aug 2019 19:33:42: 3000000 INFO @ Mon, 12 Aug 2019 19:33:42: 3000000 INFO @ Mon, 12 Aug 2019 19:33:51: 4000000 INFO @ Mon, 12 Aug 2019 19:33:51: 4000000 INFO @ Mon, 12 Aug 2019 19:33:52: 4000000 INFO @ Mon, 12 Aug 2019 19:33:59: 5000000 INFO @ Mon, 12 Aug 2019 19:33:59: 5000000 INFO @ Mon, 12 Aug 2019 19:34:01: 5000000 INFO @ Mon, 12 Aug 2019 19:34:07: 6000000 INFO @ Mon, 12 Aug 2019 19:34:07: 6000000 INFO @ Mon, 12 Aug 2019 19:34:11: 6000000 INFO @ Mon, 12 Aug 2019 19:34:15: 7000000 INFO @ Mon, 12 Aug 2019 19:34:15: 7000000 INFO @ Mon, 12 Aug 2019 19:34:20: 7000000 INFO @ Mon, 12 Aug 2019 19:34:23: 8000000 INFO @ Mon, 12 Aug 2019 19:34:24: 8000000 INFO @ Mon, 12 Aug 2019 19:34:29: 8000000 INFO @ Mon, 12 Aug 2019 19:34:31: 9000000 INFO @ Mon, 12 Aug 2019 19:34:32: 9000000 INFO @ Mon, 12 Aug 2019 19:34:39: 9000000 INFO @ Mon, 12 Aug 2019 19:34:39: 10000000 INFO @ Mon, 12 Aug 2019 19:34:40: 10000000 INFO @ Mon, 12 Aug 2019 19:34:48: 11000000 INFO @ Mon, 12 Aug 2019 19:34:48: 10000000 INFO @ Mon, 12 Aug 2019 19:34:48: 11000000 INFO @ Mon, 12 Aug 2019 19:34:57: 12000000 INFO @ Mon, 12 Aug 2019 19:34:57: 12000000 INFO @ Mon, 12 Aug 2019 19:34:58: 11000000 INFO @ Mon, 12 Aug 2019 19:35:05: 13000000 INFO @ Mon, 12 Aug 2019 19:35:05: 13000000 INFO @ Mon, 12 Aug 2019 19:35:08: 12000000 INFO @ Mon, 12 Aug 2019 19:35:13: 14000000 INFO @ Mon, 12 Aug 2019 19:35:14: 14000000 INFO @ Mon, 12 Aug 2019 19:35:18: 13000000 INFO @ Mon, 12 Aug 2019 19:35:21: 15000000 INFO @ Mon, 12 Aug 2019 19:35:22: 15000000 INFO @ Mon, 12 Aug 2019 19:35:27: 14000000 INFO @ Mon, 12 Aug 2019 19:35:29: 16000000 INFO @ Mon, 12 Aug 2019 19:35:30: 16000000 INFO @ Mon, 12 Aug 2019 19:35:36: 15000000 INFO @ Mon, 12 Aug 2019 19:35:38: 17000000 INFO @ Mon, 12 Aug 2019 19:35:38: 17000000 INFO @ Mon, 12 Aug 2019 19:35:46: 16000000 INFO @ Mon, 12 Aug 2019 19:35:46: 18000000 INFO @ Mon, 12 Aug 2019 19:35:46: 18000000 INFO @ Mon, 12 Aug 2019 19:35:54: 19000000 INFO @ Mon, 12 Aug 2019 19:35:54: 19000000 INFO @ Mon, 12 Aug 2019 19:35:55: 17000000 INFO @ Mon, 12 Aug 2019 19:36:02: 20000000 INFO @ Mon, 12 Aug 2019 19:36:02: 20000000 INFO @ Mon, 12 Aug 2019 19:36:04: 18000000 INFO @ Mon, 12 Aug 2019 19:36:10: 21000000 INFO @ Mon, 12 Aug 2019 19:36:10: 21000000 INFO @ Mon, 12 Aug 2019 19:36:14: 19000000 INFO @ Mon, 12 Aug 2019 19:36:18: 22000000 INFO @ Mon, 12 Aug 2019 19:36:19: 22000000 INFO @ Mon, 12 Aug 2019 19:36:23: 20000000 INFO @ Mon, 12 Aug 2019 19:36:26: 23000000 INFO @ Mon, 12 Aug 2019 19:36:27: 23000000 INFO @ Mon, 12 Aug 2019 19:36:32: 21000000 INFO @ Mon, 12 Aug 2019 19:36:34: 24000000 INFO @ Mon, 12 Aug 2019 19:36:35: 24000000 INFO @ Mon, 12 Aug 2019 19:36:41: 22000000 INFO @ Mon, 12 Aug 2019 19:36:42: 25000000 INFO @ Mon, 12 Aug 2019 19:36:43: 25000000 INFO @ Mon, 12 Aug 2019 19:36:50: 26000000 INFO @ Mon, 12 Aug 2019 19:36:51: 26000000 INFO @ Mon, 12 Aug 2019 19:36:51: 23000000 INFO @ Mon, 12 Aug 2019 19:36:58: 27000000 INFO @ Mon, 12 Aug 2019 19:36:59: 27000000 INFO @ Mon, 12 Aug 2019 19:37:00: 24000000 INFO @ Mon, 12 Aug 2019 19:37:06: 28000000 INFO @ Mon, 12 Aug 2019 19:37:07: 28000000 INFO @ Mon, 12 Aug 2019 19:37:09: 25000000 INFO @ Mon, 12 Aug 2019 19:37:14: 29000000 INFO @ Mon, 12 Aug 2019 19:37:15: 29000000 INFO @ Mon, 12 Aug 2019 19:37:18: 26000000 INFO @ Mon, 12 Aug 2019 19:37:22: 30000000 INFO @ Mon, 12 Aug 2019 19:37:23: 30000000 INFO @ Mon, 12 Aug 2019 19:37:27: 27000000 INFO @ Mon, 12 Aug 2019 19:37:30: 31000000 INFO @ Mon, 12 Aug 2019 19:37:31: 31000000 INFO @ Mon, 12 Aug 2019 19:37:37: 28000000 INFO @ Mon, 12 Aug 2019 19:37:38: 32000000 INFO @ Mon, 12 Aug 2019 19:37:39: 32000000 INFO @ Mon, 12 Aug 2019 19:37:46: 29000000 INFO @ Mon, 12 Aug 2019 19:37:46: 33000000 INFO @ Mon, 12 Aug 2019 19:37:47: 33000000 INFO @ Mon, 12 Aug 2019 19:37:54: 34000000 INFO @ Mon, 12 Aug 2019 19:37:55: 34000000 INFO @ Mon, 12 Aug 2019 19:37:55: 30000000 INFO @ Mon, 12 Aug 2019 19:38:02: 35000000 INFO @ Mon, 12 Aug 2019 19:38:03: 35000000 INFO @ Mon, 12 Aug 2019 19:38:04: 31000000 INFO @ Mon, 12 Aug 2019 19:38:10: 36000000 INFO @ Mon, 12 Aug 2019 19:38:11: 36000000 INFO @ Mon, 12 Aug 2019 19:38:14: 32000000 INFO @ Mon, 12 Aug 2019 19:38:15: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 19:38:15: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 19:38:15: #1 total tags in treatment: 36621594 INFO @ Mon, 12 Aug 2019 19:38:15: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 19:38:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 19:38:16: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 19:38:16: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 19:38:16: #1 total tags in treatment: 36621594 INFO @ Mon, 12 Aug 2019 19:38:16: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 19:38:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 19:38:16: #1 tags after filtering in treatment: 36621594 INFO @ Mon, 12 Aug 2019 19:38:16: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 19:38:16: #1 finished! INFO @ Mon, 12 Aug 2019 19:38:16: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 19:38:16: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 19:38:17: #1 tags after filtering in treatment: 36621594 INFO @ Mon, 12 Aug 2019 19:38:17: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 19:38:17: #1 finished! INFO @ Mon, 12 Aug 2019 19:38:17: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 19:38:17: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 19:38:19: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 19:38:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 19:38:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494862/SRX494862.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494862/SRX494862.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494862/SRX494862.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494862/SRX494862.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 19:38:20: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 19:38:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 19:38:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494862/SRX494862.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494862/SRX494862.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494862/SRX494862.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494862/SRX494862.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 19:38:23: 33000000 INFO @ Mon, 12 Aug 2019 19:38:32: 34000000 INFO @ Mon, 12 Aug 2019 19:38:41: 35000000 INFO @ Mon, 12 Aug 2019 19:38:51: 36000000 INFO @ Mon, 12 Aug 2019 19:38:57: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 19:38:57: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 19:38:57: #1 total tags in treatment: 36621594 INFO @ Mon, 12 Aug 2019 19:38:57: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 19:38:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 19:38:57: #1 tags after filtering in treatment: 36621594 INFO @ Mon, 12 Aug 2019 19:38:57: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 19:38:57: #1 finished! INFO @ Mon, 12 Aug 2019 19:38:57: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 19:38:57: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 19:39:00: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 19:39:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 19:39:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX494862/SRX494862.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494862/SRX494862.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494862/SRX494862.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX494862/SRX494862.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。