Job ID = 6497407 SRX = SRX494819 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T21:48:05 prefetch.2.10.7: 1) Downloading 'SRR1198351'... 2020-06-25T21:48:40 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T21:49:29 prefetch.2.10.7: HTTPS download succeed 2020-06-25T21:49:29 prefetch.2.10.7: 'SRR1198351' is valid 2020-06-25T21:49:29 prefetch.2.10.7: 1) 'SRR1198351' was downloaded successfully Read 9807251 spots for SRR1198351/SRR1198351.sra Written 9807251 spots for SRR1198351/SRR1198351.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:45 9807251 reads; of these: 9807251 (100.00%) were unpaired; of these: 294262 (3.00%) aligned 0 times 7729445 (78.81%) aligned exactly 1 time 1783544 (18.19%) aligned >1 times 97.00% overall alignment rate Time searching: 00:01:47 Overall time: 00:01:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 784368 / 9512989 = 0.0825 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 06:54:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494819/SRX494819.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494819/SRX494819.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494819/SRX494819.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494819/SRX494819.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 06:54:57: #1 read tag files... INFO @ Fri, 26 Jun 2020 06:54:57: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 06:55:02: 1000000 INFO @ Fri, 26 Jun 2020 06:55:08: 2000000 INFO @ Fri, 26 Jun 2020 06:55:13: 3000000 INFO @ Fri, 26 Jun 2020 06:55:19: 4000000 INFO @ Fri, 26 Jun 2020 06:55:24: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 06:55:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494819/SRX494819.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494819/SRX494819.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494819/SRX494819.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494819/SRX494819.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 06:55:26: #1 read tag files... INFO @ Fri, 26 Jun 2020 06:55:26: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 06:55:30: 6000000 INFO @ Fri, 26 Jun 2020 06:55:32: 1000000 INFO @ Fri, 26 Jun 2020 06:55:36: 7000000 INFO @ Fri, 26 Jun 2020 06:55:37: 2000000 INFO @ Fri, 26 Jun 2020 06:55:41: 8000000 INFO @ Fri, 26 Jun 2020 06:55:42: 3000000 INFO @ Fri, 26 Jun 2020 06:55:45: #1 tag size is determined as 36 bps INFO @ Fri, 26 Jun 2020 06:55:45: #1 tag size = 36 INFO @ Fri, 26 Jun 2020 06:55:45: #1 total tags in treatment: 8728621 INFO @ Fri, 26 Jun 2020 06:55:45: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 06:55:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 06:55:46: #1 tags after filtering in treatment: 8728621 INFO @ Fri, 26 Jun 2020 06:55:46: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 06:55:46: #1 finished! INFO @ Fri, 26 Jun 2020 06:55:46: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 06:55:46: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 06:55:46: #2 number of paired peaks: 342 WARNING @ Fri, 26 Jun 2020 06:55:46: Fewer paired peaks (342) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 342 pairs to build model! INFO @ Fri, 26 Jun 2020 06:55:46: start model_add_line... INFO @ Fri, 26 Jun 2020 06:55:46: start X-correlation... INFO @ Fri, 26 Jun 2020 06:55:46: end of X-cor INFO @ Fri, 26 Jun 2020 06:55:46: #2 finished! INFO @ Fri, 26 Jun 2020 06:55:46: #2 predicted fragment length is 36 bps INFO @ Fri, 26 Jun 2020 06:55:46: #2 alternative fragment length(s) may be 3,36,554 bps INFO @ Fri, 26 Jun 2020 06:55:46: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX494819/SRX494819.05_model.r WARNING @ Fri, 26 Jun 2020 06:55:46: #2 Since the d (36) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 26 Jun 2020 06:55:46: #2 You may need to consider one of the other alternative d(s): 3,36,554 WARNING @ Fri, 26 Jun 2020 06:55:46: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 26 Jun 2020 06:55:46: #3 Call peaks... INFO @ Fri, 26 Jun 2020 06:55:46: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 26 Jun 2020 06:55:47: 4000000 INFO @ Fri, 26 Jun 2020 06:55:52: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 06:55:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX494819/SRX494819.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX494819/SRX494819.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX494819/SRX494819.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX494819/SRX494819.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 06:55:56: #1 read tag files... INFO @ Fri, 26 Jun 2020 06:55:56: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 06:55:57: 6000000 INFO @ Fri, 26 Jun 2020 06:56:02: 1000000 INFO @ Fri, 26 Jun 2020 06:56:03: 7000000 INFO @ Fri, 26 Jun 2020 06:56:06: #3 Call peaks for each chromosome... INFO @ Fri, 26 Jun 2020 06:56:08: 8000000 INFO @ Fri, 26 Jun 2020 06:56:08: 2000000 INFO @ Fri, 26 Jun 2020 06:56:12: #1 tag size is determined as 36 bps INFO @ Fri, 26 Jun 2020 06:56:12: #1 tag size = 36 INFO @ Fri, 26 Jun 2020 06:56:12: #1 total tags in treatment: 8728621 INFO @ Fri, 26 Jun 2020 06:56:12: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 06:56:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 06:56:12: #1 tags after filtering in treatment: 8728621 INFO @ Fri, 26 Jun 2020 06:56:12: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 06:56:12: #1 finished! INFO @ Fri, 26 Jun 2020 06:56:12: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 06:56:12: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 06:56:13: #2 number of paired peaks: 342 WARNING @ Fri, 26 Jun 2020 06:56:13: Fewer paired peaks (342) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 342 pairs to build model! INFO @ Fri, 26 Jun 2020 06:56:13: start model_add_line... INFO @ Fri, 26 Jun 2020 06:56:13: start X-correlation... INFO @ Fri, 26 Jun 2020 06:56:13: end of X-cor INFO @ Fri, 26 Jun 2020 06:56:13: #2 finished! INFO @ Fri, 26 Jun 2020 06:56:13: #2 predicted fragment length is 36 bps INFO @ Fri, 26 Jun 2020 06:56:13: #2 alternative fragment length(s) may be 3,36,554 bps INFO @ Fri, 26 Jun 2020 06:56:13: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX494819/SRX494819.10_model.r WARNING @ Fri, 26 Jun 2020 06:56:13: #2 Since the d (36) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 26 Jun 2020 06:56:13: #2 You may need to consider one of the other alternative d(s): 3,36,554 WARNING @ Fri, 26 Jun 2020 06:56:13: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 26 Jun 2020 06:56:13: #3 Call peaks... INFO @ Fri, 26 Jun 2020 06:56:13: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 26 Jun 2020 06:56:14: 3000000 INFO @ Fri, 26 Jun 2020 06:56:16: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX494819/SRX494819.05_peaks.xls INFO @ Fri, 26 Jun 2020 06:56:19: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX494819/SRX494819.05_peaks.narrowPeak INFO @ Fri, 26 Jun 2020 06:56:19: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX494819/SRX494819.05_summits.bed INFO @ Fri, 26 Jun 2020 06:56:20: 4000000 INFO @ Fri, 26 Jun 2020 06:56:20: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (1209 records, 4 fields): 1496 millis CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 06:56:25: 5000000 INFO @ Fri, 26 Jun 2020 06:56:31: 6000000 INFO @ Fri, 26 Jun 2020 06:56:32: #3 Call peaks for each chromosome... BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 06:56:37: 7000000 INFO @ Fri, 26 Jun 2020 06:56:42: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX494819/SRX494819.10_peaks.xls INFO @ Fri, 26 Jun 2020 06:56:42: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX494819/SRX494819.10_peaks.narrowPeak INFO @ Fri, 26 Jun 2020 06:56:42: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX494819/SRX494819.10_summits.bed INFO @ Fri, 26 Jun 2020 06:56:42: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (336 records, 4 fields): 2 millis INFO @ Fri, 26 Jun 2020 06:56:42: 8000000 CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 06:56:47: #1 tag size is determined as 36 bps INFO @ Fri, 26 Jun 2020 06:56:47: #1 tag size = 36 INFO @ Fri, 26 Jun 2020 06:56:47: #1 total tags in treatment: 8728621 INFO @ Fri, 26 Jun 2020 06:56:47: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 06:56:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 06:56:47: #1 tags after filtering in treatment: 8728621 INFO @ Fri, 26 Jun 2020 06:56:47: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 06:56:47: #1 finished! INFO @ Fri, 26 Jun 2020 06:56:47: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 06:56:47: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 06:56:47: #2 number of paired peaks: 342 WARNING @ Fri, 26 Jun 2020 06:56:47: Fewer paired peaks (342) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 342 pairs to build model! INFO @ Fri, 26 Jun 2020 06:56:47: start model_add_line... INFO @ Fri, 26 Jun 2020 06:56:48: start X-correlation... INFO @ Fri, 26 Jun 2020 06:56:48: end of X-cor INFO @ Fri, 26 Jun 2020 06:56:48: #2 finished! INFO @ Fri, 26 Jun 2020 06:56:48: #2 predicted fragment length is 36 bps INFO @ Fri, 26 Jun 2020 06:56:48: #2 alternative fragment length(s) may be 3,36,554 bps INFO @ Fri, 26 Jun 2020 06:56:48: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX494819/SRX494819.20_model.r WARNING @ Fri, 26 Jun 2020 06:56:48: #2 Since the d (36) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 26 Jun 2020 06:56:48: #2 You may need to consider one of the other alternative d(s): 3,36,554 WARNING @ Fri, 26 Jun 2020 06:56:48: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 26 Jun 2020 06:56:48: #3 Call peaks... INFO @ Fri, 26 Jun 2020 06:56:48: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Fri, 26 Jun 2020 06:57:08: #3 Call peaks for each chromosome... INFO @ Fri, 26 Jun 2020 06:57:17: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX494819/SRX494819.20_peaks.xls INFO @ Fri, 26 Jun 2020 06:57:17: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX494819/SRX494819.20_peaks.narrowPeak INFO @ Fri, 26 Jun 2020 06:57:17: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX494819/SRX494819.20_summits.bed INFO @ Fri, 26 Jun 2020 06:57:17: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (102 records, 4 fields): 1 millis CompletedMACS2peakCalling