Job ID = 1292480 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 2,533,543 reads read : 2,533,543 reads written : 2,533,543 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:40 2533543 reads; of these: 2533543 (100.00%) were unpaired; of these: 215234 (8.50%) aligned 0 times 1799989 (71.05%) aligned exactly 1 time 518320 (20.46%) aligned >1 times 91.50% overall alignment rate Time searching: 00:00:40 Overall time: 00:00:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 390848 / 2318309 = 0.1686 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 19:06:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX466467/SRX466467.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX466467/SRX466467.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX466467/SRX466467.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX466467/SRX466467.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 19:06:48: #1 read tag files... INFO @ Sun, 02 Jun 2019 19:06:48: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 19:06:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX466467/SRX466467.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX466467/SRX466467.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX466467/SRX466467.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX466467/SRX466467.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 19:06:48: #1 read tag files... INFO @ Sun, 02 Jun 2019 19:06:48: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 19:06:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX466467/SRX466467.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX466467/SRX466467.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX466467/SRX466467.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX466467/SRX466467.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 19:06:48: #1 read tag files... INFO @ Sun, 02 Jun 2019 19:06:48: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 19:06:56: 1000000 INFO @ Sun, 02 Jun 2019 19:06:59: 1000000 INFO @ Sun, 02 Jun 2019 19:07:00: 1000000 INFO @ Sun, 02 Jun 2019 19:07:04: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 19:07:04: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 19:07:04: #1 total tags in treatment: 1927461 INFO @ Sun, 02 Jun 2019 19:07:04: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 19:07:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 19:07:04: #1 tags after filtering in treatment: 1927461 INFO @ Sun, 02 Jun 2019 19:07:04: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 19:07:04: #1 finished! INFO @ Sun, 02 Jun 2019 19:07:04: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 19:07:04: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 19:07:04: #2 number of paired peaks: 603 WARNING @ Sun, 02 Jun 2019 19:07:04: Fewer paired peaks (603) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 603 pairs to build model! INFO @ Sun, 02 Jun 2019 19:07:04: start model_add_line... INFO @ Sun, 02 Jun 2019 19:07:04: start X-correlation... INFO @ Sun, 02 Jun 2019 19:07:04: end of X-cor INFO @ Sun, 02 Jun 2019 19:07:04: #2 finished! INFO @ Sun, 02 Jun 2019 19:07:04: #2 predicted fragment length is 48 bps INFO @ Sun, 02 Jun 2019 19:07:04: #2 alternative fragment length(s) may be 48,482 bps INFO @ Sun, 02 Jun 2019 19:07:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX466467/SRX466467.05_model.r WARNING @ Sun, 02 Jun 2019 19:07:04: #2 Since the d (48) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 19:07:04: #2 You may need to consider one of the other alternative d(s): 48,482 WARNING @ Sun, 02 Jun 2019 19:07:04: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 19:07:04: #3 Call peaks... INFO @ Sun, 02 Jun 2019 19:07:04: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 19:07:11: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 19:07:11: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 19:07:11: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 19:07:11: #1 total tags in treatment: 1927461 INFO @ Sun, 02 Jun 2019 19:07:11: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 19:07:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 19:07:11: #1 tags after filtering in treatment: 1927461 INFO @ Sun, 02 Jun 2019 19:07:11: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 19:07:11: #1 finished! INFO @ Sun, 02 Jun 2019 19:07:11: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 19:07:11: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 19:07:11: #2 number of paired peaks: 603 WARNING @ Sun, 02 Jun 2019 19:07:11: Fewer paired peaks (603) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 603 pairs to build model! INFO @ Sun, 02 Jun 2019 19:07:11: start model_add_line... INFO @ Sun, 02 Jun 2019 19:07:11: start X-correlation... INFO @ Sun, 02 Jun 2019 19:07:11: end of X-cor INFO @ Sun, 02 Jun 2019 19:07:11: #2 finished! INFO @ Sun, 02 Jun 2019 19:07:11: #2 predicted fragment length is 48 bps INFO @ Sun, 02 Jun 2019 19:07:11: #2 alternative fragment length(s) may be 48,482 bps INFO @ Sun, 02 Jun 2019 19:07:11: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX466467/SRX466467.20_model.r WARNING @ Sun, 02 Jun 2019 19:07:11: #2 Since the d (48) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 19:07:11: #2 You may need to consider one of the other alternative d(s): 48,482 WARNING @ Sun, 02 Jun 2019 19:07:11: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 19:07:11: #3 Call peaks... INFO @ Sun, 02 Jun 2019 19:07:11: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 19:07:12: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 19:07:12: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 19:07:12: #1 total tags in treatment: 1927461 INFO @ Sun, 02 Jun 2019 19:07:12: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 19:07:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 19:07:12: #1 tags after filtering in treatment: 1927461 INFO @ Sun, 02 Jun 2019 19:07:12: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 19:07:12: #1 finished! INFO @ Sun, 02 Jun 2019 19:07:12: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 19:07:12: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 19:07:12: #2 number of paired peaks: 603 WARNING @ Sun, 02 Jun 2019 19:07:12: Fewer paired peaks (603) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 603 pairs to build model! INFO @ Sun, 02 Jun 2019 19:07:12: start model_add_line... INFO @ Sun, 02 Jun 2019 19:07:12: start X-correlation... INFO @ Sun, 02 Jun 2019 19:07:12: end of X-cor INFO @ Sun, 02 Jun 2019 19:07:12: #2 finished! INFO @ Sun, 02 Jun 2019 19:07:12: #2 predicted fragment length is 48 bps INFO @ Sun, 02 Jun 2019 19:07:12: #2 alternative fragment length(s) may be 48,482 bps INFO @ Sun, 02 Jun 2019 19:07:12: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX466467/SRX466467.10_model.r WARNING @ Sun, 02 Jun 2019 19:07:12: #2 Since the d (48) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 19:07:12: #2 You may need to consider one of the other alternative d(s): 48,482 WARNING @ Sun, 02 Jun 2019 19:07:12: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 19:07:12: #3 Call peaks... INFO @ Sun, 02 Jun 2019 19:07:12: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 19:07:14: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX466467/SRX466467.05_peaks.xls INFO @ Sun, 02 Jun 2019 19:07:14: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX466467/SRX466467.05_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 19:07:14: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX466467/SRX466467.05_summits.bed INFO @ Sun, 02 Jun 2019 19:07:14: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (485 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 19:07:17: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 19:07:18: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 19:07:20: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX466467/SRX466467.20_peaks.xls INFO @ Sun, 02 Jun 2019 19:07:20: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX466467/SRX466467.20_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 19:07:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX466467/SRX466467.20_summits.bed INFO @ Sun, 02 Jun 2019 19:07:20: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (105 records, 4 fields): 14 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 02 Jun 2019 19:07:21: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX466467/SRX466467.10_peaks.xls INFO @ Sun, 02 Jun 2019 19:07:21: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX466467/SRX466467.10_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 19:07:21: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX466467/SRX466467.10_summits.bed INFO @ Sun, 02 Jun 2019 19:07:21: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (282 records, 4 fields): 2 millis CompletedMACS2peakCalling BigWig に変換しました。