Job ID = 2589950 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 2,533,543 reads read : 2,533,543 reads written : 2,533,543 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:41 2533543 reads; of these: 2533543 (100.00%) were unpaired; of these: 215242 (8.50%) aligned 0 times 1800046 (71.05%) aligned exactly 1 time 518255 (20.46%) aligned >1 times 91.50% overall alignment rate Time searching: 00:00:41 Overall time: 00:00:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 390683 / 2318301 = 0.1685 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 18:52:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX466459/SRX466459.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX466459/SRX466459.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX466459/SRX466459.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX466459/SRX466459.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:52:09: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:52:09: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:52:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX466459/SRX466459.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX466459/SRX466459.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX466459/SRX466459.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX466459/SRX466459.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:52:10: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:52:10: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:52:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX466459/SRX466459.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX466459/SRX466459.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX466459/SRX466459.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX466459/SRX466459.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:52:11: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:52:11: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:52:18: 1000000 INFO @ Mon, 12 Aug 2019 18:52:18: 1000000 INFO @ Mon, 12 Aug 2019 18:52:20: 1000000 INFO @ Mon, 12 Aug 2019 18:52:25: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 18:52:25: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 18:52:25: #1 total tags in treatment: 1927618 INFO @ Mon, 12 Aug 2019 18:52:25: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:52:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:52:25: #1 tags after filtering in treatment: 1927618 INFO @ Mon, 12 Aug 2019 18:52:25: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:52:25: #1 finished! INFO @ Mon, 12 Aug 2019 18:52:25: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:52:25: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:52:25: #2 number of paired peaks: 615 WARNING @ Mon, 12 Aug 2019 18:52:25: Fewer paired peaks (615) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 615 pairs to build model! INFO @ Mon, 12 Aug 2019 18:52:25: start model_add_line... INFO @ Mon, 12 Aug 2019 18:52:26: start X-correlation... INFO @ Mon, 12 Aug 2019 18:52:26: end of X-cor INFO @ Mon, 12 Aug 2019 18:52:26: #2 finished! INFO @ Mon, 12 Aug 2019 18:52:26: #2 predicted fragment length is 48 bps INFO @ Mon, 12 Aug 2019 18:52:26: #2 alternative fragment length(s) may be 48,464 bps INFO @ Mon, 12 Aug 2019 18:52:26: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX466459/SRX466459.10_model.r WARNING @ Mon, 12 Aug 2019 18:52:26: #2 Since the d (48) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 18:52:26: #2 You may need to consider one of the other alternative d(s): 48,464 WARNING @ Mon, 12 Aug 2019 18:52:26: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 18:52:26: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:52:26: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:52:26: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 18:52:26: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 18:52:26: #1 total tags in treatment: 1927618 INFO @ Mon, 12 Aug 2019 18:52:26: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:52:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:52:26: #1 tags after filtering in treatment: 1927618 INFO @ Mon, 12 Aug 2019 18:52:26: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:52:26: #1 finished! INFO @ Mon, 12 Aug 2019 18:52:26: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:52:26: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:52:26: #2 number of paired peaks: 615 WARNING @ Mon, 12 Aug 2019 18:52:26: Fewer paired peaks (615) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 615 pairs to build model! INFO @ Mon, 12 Aug 2019 18:52:26: start model_add_line... INFO @ Mon, 12 Aug 2019 18:52:26: start X-correlation... INFO @ Mon, 12 Aug 2019 18:52:26: end of X-cor INFO @ Mon, 12 Aug 2019 18:52:26: #2 finished! INFO @ Mon, 12 Aug 2019 18:52:26: #2 predicted fragment length is 48 bps INFO @ Mon, 12 Aug 2019 18:52:26: #2 alternative fragment length(s) may be 48,464 bps INFO @ Mon, 12 Aug 2019 18:52:26: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX466459/SRX466459.05_model.r WARNING @ Mon, 12 Aug 2019 18:52:26: #2 Since the d (48) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 18:52:26: #2 You may need to consider one of the other alternative d(s): 48,464 WARNING @ Mon, 12 Aug 2019 18:52:26: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 18:52:26: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:52:26: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:52:29: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 18:52:29: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 18:52:29: #1 total tags in treatment: 1927618 INFO @ Mon, 12 Aug 2019 18:52:29: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:52:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:52:29: #1 tags after filtering in treatment: 1927618 INFO @ Mon, 12 Aug 2019 18:52:29: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:52:29: #1 finished! INFO @ Mon, 12 Aug 2019 18:52:29: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:52:29: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:52:30: #2 number of paired peaks: 615 WARNING @ Mon, 12 Aug 2019 18:52:30: Fewer paired peaks (615) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 615 pairs to build model! INFO @ Mon, 12 Aug 2019 18:52:30: start model_add_line... INFO @ Mon, 12 Aug 2019 18:52:30: start X-correlation... INFO @ Mon, 12 Aug 2019 18:52:30: end of X-cor INFO @ Mon, 12 Aug 2019 18:52:30: #2 finished! INFO @ Mon, 12 Aug 2019 18:52:30: #2 predicted fragment length is 48 bps INFO @ Mon, 12 Aug 2019 18:52:30: #2 alternative fragment length(s) may be 48,464 bps INFO @ Mon, 12 Aug 2019 18:52:30: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX466459/SRX466459.20_model.r WARNING @ Mon, 12 Aug 2019 18:52:30: #2 Since the d (48) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 18:52:30: #2 You may need to consider one of the other alternative d(s): 48,464 WARNING @ Mon, 12 Aug 2019 18:52:30: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 18:52:30: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:52:30: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:52:32: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:52:32: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:52:35: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX466459/SRX466459.10_peaks.xls INFO @ Mon, 12 Aug 2019 18:52:35: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX466459/SRX466459.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:52:35: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX466459/SRX466459.10_summits.bed INFO @ Mon, 12 Aug 2019 18:52:35: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (273 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:52:35: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX466459/SRX466459.05_peaks.xls INFO @ Mon, 12 Aug 2019 18:52:35: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX466459/SRX466459.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:52:35: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX466459/SRX466459.05_summits.bed INFO @ Mon, 12 Aug 2019 18:52:35: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (471 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:52:36: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:52:39: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX466459/SRX466459.20_peaks.xls INFO @ Mon, 12 Aug 2019 18:52:39: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX466459/SRX466459.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:52:39: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX466459/SRX466459.20_summits.bed INFO @ Mon, 12 Aug 2019 18:52:39: Done! pass1 - making usageList (6 chroms): 2 millis pass2 - checking and writing primary data (101 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。