Job ID = 12264778 SRX = SRX4082384 Genome = ce10 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 32468957 spots for SRR7164202/SRR7164202.sra Written 32468957 spots for SRR7164202/SRR7164202.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265417 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:35:22 32468957 reads; of these: 32468957 (100.00%) were paired; of these: 3322497 (10.23%) aligned concordantly 0 times 25505787 (78.55%) aligned concordantly exactly 1 time 3640673 (11.21%) aligned concordantly >1 times ---- 3322497 pairs aligned concordantly 0 times; of these: 2199033 (66.19%) aligned discordantly 1 time ---- 1123464 pairs aligned 0 times concordantly or discordantly; of these: 2246928 mates make up the pairs; of these: 1098269 (48.88%) aligned 0 times 608270 (27.07%) aligned exactly 1 time 540389 (24.05%) aligned >1 times 98.31% overall alignment rate Time searching: 00:35:22 Overall time: 00:35:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 5628354 / 27185024 = 0.2070 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:52:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX4082384/SRX4082384.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX4082384/SRX4082384.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX4082384/SRX4082384.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX4082384/SRX4082384.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:52:06: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:52:06: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:52:11: 1000000 INFO @ Sat, 03 Apr 2021 06:52:15: 2000000 INFO @ Sat, 03 Apr 2021 06:52:20: 3000000 INFO @ Sat, 03 Apr 2021 06:52:25: 4000000 INFO @ Sat, 03 Apr 2021 06:52:30: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:52:35: 6000000 INFO @ Sat, 03 Apr 2021 06:52:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX4082384/SRX4082384.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX4082384/SRX4082384.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX4082384/SRX4082384.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX4082384/SRX4082384.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:52:36: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:52:36: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:52:40: 7000000 INFO @ Sat, 03 Apr 2021 06:52:41: 1000000 INFO @ Sat, 03 Apr 2021 06:52:45: 8000000 INFO @ Sat, 03 Apr 2021 06:52:46: 2000000 INFO @ Sat, 03 Apr 2021 06:52:50: 9000000 INFO @ Sat, 03 Apr 2021 06:52:51: 3000000 INFO @ Sat, 03 Apr 2021 06:52:55: 10000000 INFO @ Sat, 03 Apr 2021 06:52:57: 4000000 INFO @ Sat, 03 Apr 2021 06:53:00: 11000000 INFO @ Sat, 03 Apr 2021 06:53:02: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:53:05: 12000000 INFO @ Sat, 03 Apr 2021 06:53:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX4082384/SRX4082384.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX4082384/SRX4082384.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX4082384/SRX4082384.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX4082384/SRX4082384.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:53:06: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:53:06: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:53:07: 6000000 INFO @ Sat, 03 Apr 2021 06:53:11: 13000000 INFO @ Sat, 03 Apr 2021 06:53:12: 1000000 INFO @ Sat, 03 Apr 2021 06:53:12: 7000000 INFO @ Sat, 03 Apr 2021 06:53:16: 14000000 INFO @ Sat, 03 Apr 2021 06:53:18: 8000000 INFO @ Sat, 03 Apr 2021 06:53:18: 2000000 INFO @ Sat, 03 Apr 2021 06:53:21: 15000000 INFO @ Sat, 03 Apr 2021 06:53:23: 9000000 INFO @ Sat, 03 Apr 2021 06:53:24: 3000000 INFO @ Sat, 03 Apr 2021 06:53:26: 16000000 INFO @ Sat, 03 Apr 2021 06:53:28: 10000000 INFO @ Sat, 03 Apr 2021 06:53:29: 4000000 INFO @ Sat, 03 Apr 2021 06:53:32: 17000000 INFO @ Sat, 03 Apr 2021 06:53:33: 11000000 INFO @ Sat, 03 Apr 2021 06:53:35: 5000000 INFO @ Sat, 03 Apr 2021 06:53:37: 18000000 INFO @ Sat, 03 Apr 2021 06:53:38: 12000000 INFO @ Sat, 03 Apr 2021 06:53:41: 6000000 INFO @ Sat, 03 Apr 2021 06:53:43: 19000000 INFO @ Sat, 03 Apr 2021 06:53:44: 13000000 INFO @ Sat, 03 Apr 2021 06:53:47: 7000000 INFO @ Sat, 03 Apr 2021 06:53:48: 20000000 INFO @ Sat, 03 Apr 2021 06:53:49: 14000000 INFO @ Sat, 03 Apr 2021 06:53:53: 8000000 INFO @ Sat, 03 Apr 2021 06:53:54: 21000000 INFO @ Sat, 03 Apr 2021 06:53:54: 15000000 INFO @ Sat, 03 Apr 2021 06:53:59: 22000000 INFO @ Sat, 03 Apr 2021 06:54:00: 9000000 INFO @ Sat, 03 Apr 2021 06:54:00: 16000000 INFO @ Sat, 03 Apr 2021 06:54:05: 23000000 INFO @ Sat, 03 Apr 2021 06:54:05: 17000000 INFO @ Sat, 03 Apr 2021 06:54:06: 10000000 INFO @ Sat, 03 Apr 2021 06:54:10: 24000000 INFO @ Sat, 03 Apr 2021 06:54:11: 18000000 INFO @ Sat, 03 Apr 2021 06:54:12: 11000000 INFO @ Sat, 03 Apr 2021 06:54:16: 25000000 INFO @ Sat, 03 Apr 2021 06:54:16: 19000000 INFO @ Sat, 03 Apr 2021 06:54:18: 12000000 INFO @ Sat, 03 Apr 2021 06:54:21: 20000000 INFO @ Sat, 03 Apr 2021 06:54:21: 26000000 INFO @ Sat, 03 Apr 2021 06:54:25: 13000000 INFO @ Sat, 03 Apr 2021 06:54:26: 21000000 INFO @ Sat, 03 Apr 2021 06:54:27: 27000000 INFO @ Sat, 03 Apr 2021 06:54:31: 14000000 INFO @ Sat, 03 Apr 2021 06:54:32: 22000000 INFO @ Sat, 03 Apr 2021 06:54:32: 28000000 INFO @ Sat, 03 Apr 2021 06:54:37: 23000000 INFO @ Sat, 03 Apr 2021 06:54:37: 15000000 INFO @ Sat, 03 Apr 2021 06:54:38: 29000000 INFO @ Sat, 03 Apr 2021 06:54:43: 24000000 INFO @ Sat, 03 Apr 2021 06:54:43: 30000000 INFO @ Sat, 03 Apr 2021 06:54:44: 16000000 INFO @ Sat, 03 Apr 2021 06:54:48: 25000000 INFO @ Sat, 03 Apr 2021 06:54:49: 31000000 INFO @ Sat, 03 Apr 2021 06:54:50: 17000000 INFO @ Sat, 03 Apr 2021 06:54:53: 26000000 INFO @ Sat, 03 Apr 2021 06:54:55: 32000000 INFO @ Sat, 03 Apr 2021 06:54:56: 18000000 INFO @ Sat, 03 Apr 2021 06:54:59: 27000000 INFO @ Sat, 03 Apr 2021 06:55:00: 33000000 INFO @ Sat, 03 Apr 2021 06:55:03: 19000000 INFO @ Sat, 03 Apr 2021 06:55:04: 28000000 INFO @ Sat, 03 Apr 2021 06:55:05: 34000000 INFO @ Sat, 03 Apr 2021 06:55:09: 29000000 INFO @ Sat, 03 Apr 2021 06:55:09: 20000000 INFO @ Sat, 03 Apr 2021 06:55:11: 35000000 INFO @ Sat, 03 Apr 2021 06:55:14: 30000000 INFO @ Sat, 03 Apr 2021 06:55:15: 21000000 INFO @ Sat, 03 Apr 2021 06:55:16: 36000000 INFO @ Sat, 03 Apr 2021 06:55:20: 31000000 INFO @ Sat, 03 Apr 2021 06:55:22: 37000000 INFO @ Sat, 03 Apr 2021 06:55:22: 22000000 INFO @ Sat, 03 Apr 2021 06:55:25: 32000000 INFO @ Sat, 03 Apr 2021 06:55:27: 38000000 INFO @ Sat, 03 Apr 2021 06:55:29: 23000000 INFO @ Sat, 03 Apr 2021 06:55:29: 33000000 INFO @ Sat, 03 Apr 2021 06:55:32: 39000000 INFO @ Sat, 03 Apr 2021 06:55:35: 34000000 INFO @ Sat, 03 Apr 2021 06:55:35: 24000000 INFO @ Sat, 03 Apr 2021 06:55:37: 40000000 INFO @ Sat, 03 Apr 2021 06:55:40: 35000000 INFO @ Sat, 03 Apr 2021 06:55:42: 25000000 INFO @ Sat, 03 Apr 2021 06:55:42: 41000000 INFO @ Sat, 03 Apr 2021 06:55:45: 36000000 INFO @ Sat, 03 Apr 2021 06:55:47: 42000000 INFO @ Sat, 03 Apr 2021 06:55:49: 26000000 INFO @ Sat, 03 Apr 2021 06:55:50: 37000000 INFO @ Sat, 03 Apr 2021 06:55:53: 43000000 INFO @ Sat, 03 Apr 2021 06:55:55: 38000000 INFO @ Sat, 03 Apr 2021 06:55:55: 27000000 INFO @ Sat, 03 Apr 2021 06:55:58: 44000000 INFO @ Sat, 03 Apr 2021 06:56:00: 39000000 INFO @ Sat, 03 Apr 2021 06:56:02: 28000000 INFO @ Sat, 03 Apr 2021 06:56:03: 45000000 INFO @ Sat, 03 Apr 2021 06:56:05: 40000000 INFO @ Sat, 03 Apr 2021 06:56:08: 29000000 INFO @ Sat, 03 Apr 2021 06:56:09: 46000000 INFO @ Sat, 03 Apr 2021 06:56:10: 41000000 INFO @ Sat, 03 Apr 2021 06:56:14: 47000000 INFO @ Sat, 03 Apr 2021 06:56:15: 30000000 INFO @ Sat, 03 Apr 2021 06:56:15: 42000000 INFO @ Sat, 03 Apr 2021 06:56:20: 43000000 INFO @ Sat, 03 Apr 2021 06:56:21: 48000000 INFO @ Sat, 03 Apr 2021 06:56:22: 31000000 INFO @ Sat, 03 Apr 2021 06:56:25: 44000000 INFO @ Sat, 03 Apr 2021 06:56:26: 49000000 INFO @ Sat, 03 Apr 2021 06:56:28: 32000000 INFO @ Sat, 03 Apr 2021 06:56:30: 45000000 INFO @ Sat, 03 Apr 2021 06:56:31: 50000000 INFO @ Sat, 03 Apr 2021 06:56:35: 46000000 INFO @ Sat, 03 Apr 2021 06:56:35: 33000000 INFO @ Sat, 03 Apr 2021 06:56:36: 51000000 INFO @ Sat, 03 Apr 2021 06:56:40: 47000000 INFO @ Sat, 03 Apr 2021 06:56:41: 52000000 INFO @ Sat, 03 Apr 2021 06:56:42: 34000000 INFO @ Sat, 03 Apr 2021 06:56:44: #1 tag size is determined as 47 bps INFO @ Sat, 03 Apr 2021 06:56:44: #1 tag size = 47 INFO @ Sat, 03 Apr 2021 06:56:44: #1 total tags in treatment: 23626318 INFO @ Sat, 03 Apr 2021 06:56:44: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:56:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 06:56:45: 48000000 INFO @ Sat, 03 Apr 2021 06:56:45: #1 tags after filtering in treatment: 14781090 INFO @ Sat, 03 Apr 2021 06:56:45: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 03 Apr 2021 06:56:45: #1 finished! INFO @ Sat, 03 Apr 2021 06:56:45: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:56:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:56:46: #2 number of paired peaks: 328 WARNING @ Sat, 03 Apr 2021 06:56:46: Fewer paired peaks (328) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 328 pairs to build model! INFO @ Sat, 03 Apr 2021 06:56:46: start model_add_line... INFO @ Sat, 03 Apr 2021 06:56:46: start X-correlation... INFO @ Sat, 03 Apr 2021 06:56:46: end of X-cor INFO @ Sat, 03 Apr 2021 06:56:46: #2 finished! INFO @ Sat, 03 Apr 2021 06:56:46: #2 predicted fragment length is 67 bps INFO @ Sat, 03 Apr 2021 06:56:46: #2 alternative fragment length(s) may be 67 bps INFO @ Sat, 03 Apr 2021 06:56:46: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX4082384/SRX4082384.05_model.r WARNING @ Sat, 03 Apr 2021 06:56:46: #2 Since the d (67) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 06:56:46: #2 You may need to consider one of the other alternative d(s): 67 WARNING @ Sat, 03 Apr 2021 06:56:46: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 06:56:46: #3 Call peaks... INFO @ Sat, 03 Apr 2021 06:56:46: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 06:56:48: 35000000 INFO @ Sat, 03 Apr 2021 06:56:49: 49000000 INFO @ Sat, 03 Apr 2021 06:56:54: 50000000 INFO @ Sat, 03 Apr 2021 06:56:55: 36000000 INFO @ Sat, 03 Apr 2021 06:56:59: 51000000 INFO @ Sat, 03 Apr 2021 06:57:02: 37000000 INFO @ Sat, 03 Apr 2021 06:57:03: 52000000 INFO @ Sat, 03 Apr 2021 06:57:06: #1 tag size is determined as 47 bps INFO @ Sat, 03 Apr 2021 06:57:06: #1 tag size = 47 INFO @ Sat, 03 Apr 2021 06:57:06: #1 total tags in treatment: 23626318 INFO @ Sat, 03 Apr 2021 06:57:06: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:57:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:57:07: #1 tags after filtering in treatment: 14781090 INFO @ Sat, 03 Apr 2021 06:57:07: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 03 Apr 2021 06:57:07: #1 finished! INFO @ Sat, 03 Apr 2021 06:57:07: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:57:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:57:08: #2 number of paired peaks: 328 WARNING @ Sat, 03 Apr 2021 06:57:08: Fewer paired peaks (328) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 328 pairs to build model! INFO @ Sat, 03 Apr 2021 06:57:08: start model_add_line... INFO @ Sat, 03 Apr 2021 06:57:08: start X-correlation... INFO @ Sat, 03 Apr 2021 06:57:08: end of X-cor INFO @ Sat, 03 Apr 2021 06:57:08: #2 finished! INFO @ Sat, 03 Apr 2021 06:57:08: #2 predicted fragment length is 67 bps INFO @ Sat, 03 Apr 2021 06:57:08: #2 alternative fragment length(s) may be 67 bps INFO @ Sat, 03 Apr 2021 06:57:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX4082384/SRX4082384.10_model.r WARNING @ Sat, 03 Apr 2021 06:57:08: #2 Since the d (67) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 06:57:08: #2 You may need to consider one of the other alternative d(s): 67 WARNING @ Sat, 03 Apr 2021 06:57:08: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 06:57:08: #3 Call peaks... INFO @ Sat, 03 Apr 2021 06:57:08: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 06:57:09: 38000000 INFO @ Sat, 03 Apr 2021 06:57:12: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 06:57:16: 39000000 INFO @ Sat, 03 Apr 2021 06:57:23: 40000000 INFO @ Sat, 03 Apr 2021 06:57:27: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX4082384/SRX4082384.05_peaks.xls INFO @ Sat, 03 Apr 2021 06:57:27: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX4082384/SRX4082384.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 06:57:27: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX4082384/SRX4082384.05_summits.bed INFO @ Sat, 03 Apr 2021 06:57:27: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (7974 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 06:57:30: 41000000 INFO @ Sat, 03 Apr 2021 06:57:34: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 06:57:37: 42000000 INFO @ Sat, 03 Apr 2021 06:57:44: 43000000 INFO @ Sat, 03 Apr 2021 06:57:47: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX4082384/SRX4082384.10_peaks.xls INFO @ Sat, 03 Apr 2021 06:57:47: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX4082384/SRX4082384.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 06:57:47: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX4082384/SRX4082384.10_summits.bed INFO @ Sat, 03 Apr 2021 06:57:47: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (4557 records, 4 fields): 7 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 06:57:51: 44000000 INFO @ Sat, 03 Apr 2021 06:57:58: 45000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 06:58:05: 46000000 INFO @ Sat, 03 Apr 2021 06:58:12: 47000000 INFO @ Sat, 03 Apr 2021 06:58:20: 48000000 INFO @ Sat, 03 Apr 2021 06:58:27: 49000000 INFO @ Sat, 03 Apr 2021 06:58:34: 50000000 INFO @ Sat, 03 Apr 2021 06:58:41: 51000000 INFO @ Sat, 03 Apr 2021 06:58:48: 52000000 INFO @ Sat, 03 Apr 2021 06:58:53: #1 tag size is determined as 47 bps INFO @ Sat, 03 Apr 2021 06:58:53: #1 tag size = 47 INFO @ Sat, 03 Apr 2021 06:58:53: #1 total tags in treatment: 23626318 INFO @ Sat, 03 Apr 2021 06:58:53: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:58:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:58:53: #1 tags after filtering in treatment: 14781090 INFO @ Sat, 03 Apr 2021 06:58:53: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 03 Apr 2021 06:58:53: #1 finished! INFO @ Sat, 03 Apr 2021 06:58:53: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:58:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:58:55: #2 number of paired peaks: 328 WARNING @ Sat, 03 Apr 2021 06:58:55: Fewer paired peaks (328) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 328 pairs to build model! INFO @ Sat, 03 Apr 2021 06:58:55: start model_add_line... INFO @ Sat, 03 Apr 2021 06:58:55: start X-correlation... INFO @ Sat, 03 Apr 2021 06:58:55: end of X-cor INFO @ Sat, 03 Apr 2021 06:58:55: #2 finished! INFO @ Sat, 03 Apr 2021 06:58:55: #2 predicted fragment length is 67 bps INFO @ Sat, 03 Apr 2021 06:58:55: #2 alternative fragment length(s) may be 67 bps INFO @ Sat, 03 Apr 2021 06:58:55: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX4082384/SRX4082384.20_model.r WARNING @ Sat, 03 Apr 2021 06:58:55: #2 Since the d (67) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 06:58:55: #2 You may need to consider one of the other alternative d(s): 67 WARNING @ Sat, 03 Apr 2021 06:58:55: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 06:58:55: #3 Call peaks... INFO @ Sat, 03 Apr 2021 06:58:55: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 06:59:23: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 06:59:40: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX4082384/SRX4082384.20_peaks.xls INFO @ Sat, 03 Apr 2021 06:59:40: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX4082384/SRX4082384.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 06:59:40: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX4082384/SRX4082384.20_summits.bed INFO @ Sat, 03 Apr 2021 06:59:40: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (1910 records, 4 fields): 4 millis CompletedMACS2peakCalling