Job ID = 11158461 sra ファイルのダウンロード中... Completed: 3142335K bytes transferred in 368 seconds (69918K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 40198550 spots for /home/okishinya/chipatlas/results/ce10/SRX4012475/SRR7082716.sra Written 40198550 spots for /home/okishinya/chipatlas/results/ce10/SRX4012475/SRR7082716.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:02:50 40198550 reads; of these: 40198550 (100.00%) were paired; of these: 1176770 (2.93%) aligned concordantly 0 times 34000616 (84.58%) aligned concordantly exactly 1 time 5021164 (12.49%) aligned concordantly >1 times ---- 1176770 pairs aligned concordantly 0 times; of these: 422479 (35.90%) aligned discordantly 1 time ---- 754291 pairs aligned 0 times concordantly or discordantly; of these: 1508582 mates make up the pairs; of these: 658775 (43.67%) aligned 0 times 514451 (34.10%) aligned exactly 1 time 335356 (22.23%) aligned >1 times 99.18% overall alignment rate Time searching: 01:02:50 Overall time: 01:02:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 36 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 1515245 / 39111601 = 0.0387 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 04 Sep 2018 10:06:44: # Command line: callpeak -t SRX4012475.bam -f BAM -g ce -n SRX4012475.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4012475.10 # format = BAM # ChIP-seq file = ['SRX4012475.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 04 Sep 2018 10:06:44: # Command line: callpeak -t SRX4012475.bam -f BAM -g ce -n SRX4012475.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4012475.05 # format = BAM # ChIP-seq file = ['SRX4012475.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 04 Sep 2018 10:06:44: # Command line: callpeak -t SRX4012475.bam -f BAM -g ce -n SRX4012475.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4012475.20 # format = BAM # ChIP-seq file = ['SRX4012475.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 04 Sep 2018 10:06:44: #1 read tag files... INFO @ Tue, 04 Sep 2018 10:06:44: #1 read tag files... INFO @ Tue, 04 Sep 2018 10:06:44: #1 read tag files... INFO @ Tue, 04 Sep 2018 10:06:44: #1 read treatment tags... INFO @ Tue, 04 Sep 2018 10:06:44: #1 read treatment tags... INFO @ Tue, 04 Sep 2018 10:06:44: #1 read treatment tags... INFO @ Tue, 04 Sep 2018 10:06:51: 1000000 INFO @ Tue, 04 Sep 2018 10:06:51: 1000000 INFO @ Tue, 04 Sep 2018 10:06:51: 1000000 INFO @ Tue, 04 Sep 2018 10:06:58: 2000000 INFO @ Tue, 04 Sep 2018 10:06:58: 2000000 INFO @ Tue, 04 Sep 2018 10:06:58: 2000000 INFO @ Tue, 04 Sep 2018 10:07:04: 3000000 INFO @ Tue, 04 Sep 2018 10:07:05: 3000000 INFO @ Tue, 04 Sep 2018 10:07:06: 3000000 INFO @ Tue, 04 Sep 2018 10:07:11: 4000000 INFO @ Tue, 04 Sep 2018 10:07:12: 4000000 INFO @ Tue, 04 Sep 2018 10:07:14: 4000000 INFO @ Tue, 04 Sep 2018 10:07:17: 5000000 INFO @ Tue, 04 Sep 2018 10:07:20: 5000000 INFO @ Tue, 04 Sep 2018 10:07:22: 5000000 INFO @ Tue, 04 Sep 2018 10:07:24: 6000000 INFO @ Tue, 04 Sep 2018 10:07:27: 6000000 INFO @ Tue, 04 Sep 2018 10:07:30: 6000000 INFO @ Tue, 04 Sep 2018 10:07:30: 7000000 INFO @ Tue, 04 Sep 2018 10:07:34: 7000000 INFO @ Tue, 04 Sep 2018 10:07:37: 8000000 INFO @ Tue, 04 Sep 2018 10:07:38: 7000000 INFO @ Tue, 04 Sep 2018 10:07:41: 8000000 INFO @ Tue, 04 Sep 2018 10:07:43: 9000000 INFO @ Tue, 04 Sep 2018 10:07:46: 8000000 INFO @ Tue, 04 Sep 2018 10:07:49: 9000000 INFO @ Tue, 04 Sep 2018 10:07:50: 10000000 INFO @ Tue, 04 Sep 2018 10:07:54: 9000000 INFO @ Tue, 04 Sep 2018 10:07:56: 10000000 INFO @ Tue, 04 Sep 2018 10:07:56: 11000000 INFO @ Tue, 04 Sep 2018 10:08:02: 10000000 INFO @ Tue, 04 Sep 2018 10:08:03: 12000000 INFO @ Tue, 04 Sep 2018 10:08:03: 11000000 INFO @ Tue, 04 Sep 2018 10:08:09: 13000000 INFO @ Tue, 04 Sep 2018 10:08:10: 11000000 INFO @ Tue, 04 Sep 2018 10:08:11: 12000000 INFO @ Tue, 04 Sep 2018 10:08:16: 14000000 INFO @ Tue, 04 Sep 2018 10:08:18: 13000000 INFO @ Tue, 04 Sep 2018 10:08:18: 12000000 INFO @ Tue, 04 Sep 2018 10:08:23: 15000000 INFO @ Tue, 04 Sep 2018 10:08:24: 14000000 INFO @ Tue, 04 Sep 2018 10:08:25: 13000000 INFO @ Tue, 04 Sep 2018 10:08:30: 16000000 INFO @ Tue, 04 Sep 2018 10:08:31: 15000000 INFO @ Tue, 04 Sep 2018 10:08:32: 14000000 INFO @ Tue, 04 Sep 2018 10:08:37: 17000000 INFO @ Tue, 04 Sep 2018 10:08:37: 16000000 INFO @ Tue, 04 Sep 2018 10:08:39: 15000000 INFO @ Tue, 04 Sep 2018 10:08:44: 17000000 INFO @ Tue, 04 Sep 2018 10:08:44: 18000000 INFO @ Tue, 04 Sep 2018 10:08:46: 16000000 INFO @ Tue, 04 Sep 2018 10:08:50: 18000000 INFO @ Tue, 04 Sep 2018 10:08:51: 19000000 INFO @ Tue, 04 Sep 2018 10:08:53: 17000000 INFO @ Tue, 04 Sep 2018 10:08:57: 19000000 INFO @ Tue, 04 Sep 2018 10:08:59: 20000000 INFO @ Tue, 04 Sep 2018 10:09:00: 18000000 INFO @ Tue, 04 Sep 2018 10:09:04: 20000000 INFO @ Tue, 04 Sep 2018 10:09:06: 21000000 INFO @ Tue, 04 Sep 2018 10:09:07: 19000000 INFO @ Tue, 04 Sep 2018 10:09:10: 21000000 INFO @ Tue, 04 Sep 2018 10:09:13: 22000000 INFO @ Tue, 04 Sep 2018 10:09:15: 20000000 INFO @ Tue, 04 Sep 2018 10:09:17: 22000000 INFO @ Tue, 04 Sep 2018 10:09:20: 23000000 INFO @ Tue, 04 Sep 2018 10:09:22: 21000000 INFO @ Tue, 04 Sep 2018 10:09:23: 23000000 INFO @ Tue, 04 Sep 2018 10:09:27: 24000000 INFO @ Tue, 04 Sep 2018 10:09:29: 22000000 INFO @ Tue, 04 Sep 2018 10:09:30: 24000000 INFO @ Tue, 04 Sep 2018 10:09:35: 25000000 INFO @ Tue, 04 Sep 2018 10:09:36: 23000000 INFO @ Tue, 04 Sep 2018 10:09:37: 25000000 INFO @ Tue, 04 Sep 2018 10:09:42: 26000000 INFO @ Tue, 04 Sep 2018 10:09:43: 24000000 INFO @ Tue, 04 Sep 2018 10:09:43: 26000000 INFO @ Tue, 04 Sep 2018 10:09:49: 27000000 INFO @ Tue, 04 Sep 2018 10:09:50: 27000000 INFO @ Tue, 04 Sep 2018 10:09:50: 25000000 INFO @ Tue, 04 Sep 2018 10:09:56: 28000000 INFO @ Tue, 04 Sep 2018 10:09:57: 28000000 INFO @ Tue, 04 Sep 2018 10:09:58: 26000000 INFO @ Tue, 04 Sep 2018 10:10:03: 29000000 INFO @ Tue, 04 Sep 2018 10:10:03: 29000000 INFO @ Tue, 04 Sep 2018 10:10:05: 27000000 INFO @ Tue, 04 Sep 2018 10:10:10: 30000000 INFO @ Tue, 04 Sep 2018 10:10:10: 30000000 INFO @ Tue, 04 Sep 2018 10:10:12: 28000000 INFO @ Tue, 04 Sep 2018 10:10:17: 31000000 INFO @ Tue, 04 Sep 2018 10:10:17: 31000000 INFO @ Tue, 04 Sep 2018 10:10:20: 29000000 INFO @ Tue, 04 Sep 2018 10:10:24: 32000000 INFO @ Tue, 04 Sep 2018 10:10:24: 32000000 INFO @ Tue, 04 Sep 2018 10:10:28: 30000000 INFO @ Tue, 04 Sep 2018 10:10:31: 33000000 INFO @ Tue, 04 Sep 2018 10:10:31: 33000000 INFO @ Tue, 04 Sep 2018 10:10:35: 31000000 INFO @ Tue, 04 Sep 2018 10:10:37: 34000000 INFO @ Tue, 04 Sep 2018 10:10:39: 34000000 INFO @ Tue, 04 Sep 2018 10:10:42: 32000000 INFO @ Tue, 04 Sep 2018 10:10:44: 35000000 INFO @ Tue, 04 Sep 2018 10:10:46: 35000000 INFO @ Tue, 04 Sep 2018 10:10:50: 33000000 INFO @ Tue, 04 Sep 2018 10:10:50: 36000000 INFO @ Tue, 04 Sep 2018 10:10:53: 36000000 INFO @ Tue, 04 Sep 2018 10:10:57: 37000000 INFO @ Tue, 04 Sep 2018 10:10:58: 34000000 INFO @ Tue, 04 Sep 2018 10:11:00: 37000000 INFO @ Tue, 04 Sep 2018 10:11:03: 38000000 INFO @ Tue, 04 Sep 2018 10:11:06: 35000000 INFO @ Tue, 04 Sep 2018 10:11:07: 38000000 INFO @ Tue, 04 Sep 2018 10:11:10: 39000000 INFO @ Tue, 04 Sep 2018 10:11:13: 36000000 INFO @ Tue, 04 Sep 2018 10:11:14: 39000000 INFO @ Tue, 04 Sep 2018 10:11:16: 40000000 INFO @ Tue, 04 Sep 2018 10:11:21: 37000000 INFO @ Tue, 04 Sep 2018 10:11:22: 40000000 INFO @ Tue, 04 Sep 2018 10:11:23: 41000000 INFO @ Tue, 04 Sep 2018 10:11:28: 38000000 INFO @ Tue, 04 Sep 2018 10:11:29: 41000000 INFO @ Tue, 04 Sep 2018 10:11:29: 42000000 INFO @ Tue, 04 Sep 2018 10:11:36: 43000000 INFO @ Tue, 04 Sep 2018 10:11:36: 42000000 INFO @ Tue, 04 Sep 2018 10:11:36: 39000000 INFO @ Tue, 04 Sep 2018 10:11:42: 44000000 INFO @ Tue, 04 Sep 2018 10:11:43: 43000000 INFO @ Tue, 04 Sep 2018 10:11:44: 40000000 INFO @ Tue, 04 Sep 2018 10:11:49: 45000000 INFO @ Tue, 04 Sep 2018 10:11:50: 44000000 INFO @ Tue, 04 Sep 2018 10:11:52: 41000000 INFO @ Tue, 04 Sep 2018 10:11:55: 46000000 INFO @ Tue, 04 Sep 2018 10:11:58: 45000000 INFO @ Tue, 04 Sep 2018 10:12:00: 42000000 INFO @ Tue, 04 Sep 2018 10:12:02: 47000000 INFO @ Tue, 04 Sep 2018 10:12:05: 46000000 INFO @ Tue, 04 Sep 2018 10:12:07: 43000000 INFO @ Tue, 04 Sep 2018 10:12:08: 48000000 INFO @ Tue, 04 Sep 2018 10:12:12: 47000000 INFO @ Tue, 04 Sep 2018 10:12:15: 49000000 INFO @ Tue, 04 Sep 2018 10:12:15: 44000000 INFO @ Tue, 04 Sep 2018 10:12:19: 48000000 INFO @ Tue, 04 Sep 2018 10:12:21: 50000000 INFO @ Tue, 04 Sep 2018 10:12:23: 45000000 INFO @ Tue, 04 Sep 2018 10:12:26: 49000000 INFO @ Tue, 04 Sep 2018 10:12:28: 51000000 INFO @ Tue, 04 Sep 2018 10:12:30: 46000000 INFO @ Tue, 04 Sep 2018 10:12:33: 50000000 INFO @ Tue, 04 Sep 2018 10:12:34: 52000000 INFO @ Tue, 04 Sep 2018 10:12:38: 47000000 INFO @ Tue, 04 Sep 2018 10:12:41: 51000000 INFO @ Tue, 04 Sep 2018 10:12:41: 53000000 INFO @ Tue, 04 Sep 2018 10:12:45: 48000000 INFO @ Tue, 04 Sep 2018 10:12:47: 54000000 INFO @ Tue, 04 Sep 2018 10:12:48: 52000000 INFO @ Tue, 04 Sep 2018 10:12:53: 49000000 INFO @ Tue, 04 Sep 2018 10:12:54: 55000000 INFO @ Tue, 04 Sep 2018 10:12:55: 53000000 INFO @ Tue, 04 Sep 2018 10:13:00: 50000000 INFO @ Tue, 04 Sep 2018 10:13:00: 56000000 INFO @ Tue, 04 Sep 2018 10:13:02: 54000000 INFO @ Tue, 04 Sep 2018 10:13:07: 57000000 INFO @ Tue, 04 Sep 2018 10:13:08: 51000000 INFO @ Tue, 04 Sep 2018 10:13:09: 55000000 INFO @ Tue, 04 Sep 2018 10:13:13: 58000000 INFO @ Tue, 04 Sep 2018 10:13:15: 52000000 INFO @ Tue, 04 Sep 2018 10:13:16: 56000000 INFO @ Tue, 04 Sep 2018 10:13:20: 59000000 INFO @ Tue, 04 Sep 2018 10:13:23: 57000000 INFO @ Tue, 04 Sep 2018 10:13:23: 53000000 INFO @ Tue, 04 Sep 2018 10:13:26: 60000000 INFO @ Tue, 04 Sep 2018 10:13:30: 58000000 INFO @ Tue, 04 Sep 2018 10:13:30: 54000000 INFO @ Tue, 04 Sep 2018 10:13:33: 61000000 INFO @ Tue, 04 Sep 2018 10:13:37: 59000000 INFO @ Tue, 04 Sep 2018 10:13:38: 55000000 INFO @ Tue, 04 Sep 2018 10:13:39: 62000000 INFO @ Tue, 04 Sep 2018 10:13:44: 60000000 INFO @ Tue, 04 Sep 2018 10:13:46: 56000000 INFO @ Tue, 04 Sep 2018 10:13:46: 63000000 INFO @ Tue, 04 Sep 2018 10:13:51: 61000000 INFO @ Tue, 04 Sep 2018 10:13:53: 64000000 INFO @ Tue, 04 Sep 2018 10:13:53: 57000000 INFO @ Tue, 04 Sep 2018 10:13:58: 62000000 INFO @ Tue, 04 Sep 2018 10:13:59: 65000000 INFO @ Tue, 04 Sep 2018 10:14:01: 58000000 INFO @ Tue, 04 Sep 2018 10:14:05: 63000000 INFO @ Tue, 04 Sep 2018 10:14:06: 66000000 INFO @ Tue, 04 Sep 2018 10:14:08: 59000000 INFO @ Tue, 04 Sep 2018 10:14:12: 64000000 INFO @ Tue, 04 Sep 2018 10:14:12: 67000000 INFO @ Tue, 04 Sep 2018 10:14:16: 60000000 INFO @ Tue, 04 Sep 2018 10:14:19: 68000000 INFO @ Tue, 04 Sep 2018 10:14:19: 65000000 INFO @ Tue, 04 Sep 2018 10:14:24: 61000000 INFO @ Tue, 04 Sep 2018 10:14:25: 69000000 INFO @ Tue, 04 Sep 2018 10:14:26: 66000000 INFO @ Tue, 04 Sep 2018 10:14:31: 62000000 INFO @ Tue, 04 Sep 2018 10:14:32: 70000000 INFO @ Tue, 04 Sep 2018 10:14:33: 67000000 INFO @ Tue, 04 Sep 2018 10:14:38: 63000000 INFO @ Tue, 04 Sep 2018 10:14:38: 71000000 INFO @ Tue, 04 Sep 2018 10:14:40: 68000000 INFO @ Tue, 04 Sep 2018 10:14:45: 72000000 INFO @ Tue, 04 Sep 2018 10:14:46: 64000000 INFO @ Tue, 04 Sep 2018 10:14:47: 69000000 INFO @ Tue, 04 Sep 2018 10:14:51: 73000000 INFO @ Tue, 04 Sep 2018 10:14:53: 65000000 INFO @ Tue, 04 Sep 2018 10:14:54: 70000000 INFO @ Tue, 04 Sep 2018 10:14:58: 74000000 INFO @ Tue, 04 Sep 2018 10:15:01: 71000000 INFO @ Tue, 04 Sep 2018 10:15:01: 66000000 INFO @ Tue, 04 Sep 2018 10:15:04: 75000000 INFO @ Tue, 04 Sep 2018 10:15:07: 72000000 INFO @ Tue, 04 Sep 2018 10:15:08: 67000000 INFO @ Tue, 04 Sep 2018 10:15:11: 76000000 INFO @ Tue, 04 Sep 2018 10:15:14: 73000000 INFO @ Tue, 04 Sep 2018 10:15:15: 68000000 INFO @ Tue, 04 Sep 2018 10:15:16: #1 tag size is determined as 75 bps INFO @ Tue, 04 Sep 2018 10:15:16: #1 tag size = 75 INFO @ Tue, 04 Sep 2018 10:15:16: #1 total tags in treatment: 37509740 INFO @ Tue, 04 Sep 2018 10:15:16: #1 user defined the maximum tags... INFO @ Tue, 04 Sep 2018 10:15:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 04 Sep 2018 10:15:16: #1 tags after filtering in treatment: 32379503 INFO @ Tue, 04 Sep 2018 10:15:16: #1 Redundant rate of treatment: 0.14 INFO @ Tue, 04 Sep 2018 10:15:16: #1 finished! INFO @ Tue, 04 Sep 2018 10:15:16: #2 Build Peak Model... INFO @ Tue, 04 Sep 2018 10:15:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 04 Sep 2018 10:15:19: #2 number of paired peaks: 50 WARNING @ Tue, 04 Sep 2018 10:15:19: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 04 Sep 2018 10:15:19: Process for pairing-model is terminated! cat: SRX4012475.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4012475.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4012475.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4012475.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 04 Sep 2018 10:15:21: 74000000 INFO @ Tue, 04 Sep 2018 10:15:23: 69000000 INFO @ Tue, 04 Sep 2018 10:15:28: 75000000 INFO @ Tue, 04 Sep 2018 10:15:30: 70000000 INFO @ Tue, 04 Sep 2018 10:15:35: 76000000 INFO @ Tue, 04 Sep 2018 10:15:37: 71000000 INFO @ Tue, 04 Sep 2018 10:15:40: #1 tag size is determined as 75 bps INFO @ Tue, 04 Sep 2018 10:15:40: #1 tag size = 75 INFO @ Tue, 04 Sep 2018 10:15:40: #1 total tags in treatment: 37509740 INFO @ Tue, 04 Sep 2018 10:15:40: #1 user defined the maximum tags... INFO @ Tue, 04 Sep 2018 10:15:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 04 Sep 2018 10:15:41: #1 tags after filtering in treatment: 32379503 INFO @ Tue, 04 Sep 2018 10:15:41: #1 Redundant rate of treatment: 0.14 INFO @ Tue, 04 Sep 2018 10:15:41: #1 finished! INFO @ Tue, 04 Sep 2018 10:15:41: #2 Build Peak Model... INFO @ Tue, 04 Sep 2018 10:15:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 04 Sep 2018 10:15:43: #2 number of paired peaks: 50 WARNING @ Tue, 04 Sep 2018 10:15:43: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 04 Sep 2018 10:15:43: Process for pairing-model is terminated! cat: SRX4012475.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4012475.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4012475.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4012475.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 04 Sep 2018 10:15:44: 72000000 INFO @ Tue, 04 Sep 2018 10:15:50: 73000000 INFO @ Tue, 04 Sep 2018 10:15:57: 74000000 INFO @ Tue, 04 Sep 2018 10:16:03: 75000000 INFO @ Tue, 04 Sep 2018 10:16:09: 76000000 INFO @ Tue, 04 Sep 2018 10:16:14: #1 tag size is determined as 75 bps INFO @ Tue, 04 Sep 2018 10:16:14: #1 tag size = 75 INFO @ Tue, 04 Sep 2018 10:16:14: #1 total tags in treatment: 37509740 INFO @ Tue, 04 Sep 2018 10:16:14: #1 user defined the maximum tags... INFO @ Tue, 04 Sep 2018 10:16:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 04 Sep 2018 10:16:15: #1 tags after filtering in treatment: 32379503 INFO @ Tue, 04 Sep 2018 10:16:15: #1 Redundant rate of treatment: 0.14 INFO @ Tue, 04 Sep 2018 10:16:15: #1 finished! INFO @ Tue, 04 Sep 2018 10:16:15: #2 Build Peak Model... INFO @ Tue, 04 Sep 2018 10:16:15: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 04 Sep 2018 10:16:17: #2 number of paired peaks: 50 WARNING @ Tue, 04 Sep 2018 10:16:17: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 04 Sep 2018 10:16:17: Process for pairing-model is terminated! cat: SRX4012475.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4012475.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4012475.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4012475.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。