Job ID = 11158459 sra ファイルのダウンロード中... Completed: 3655911K bytes transferred in 436 seconds (68678K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 47685889 spots for /home/okishinya/chipatlas/results/ce10/SRX4012473/SRR7082714.sra Written 47685889 spots for /home/okishinya/chipatlas/results/ce10/SRX4012473/SRR7082714.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:11:03 47685889 reads; of these: 47685889 (100.00%) were paired; of these: 1774063 (3.72%) aligned concordantly 0 times 39470595 (82.77%) aligned concordantly exactly 1 time 6441231 (13.51%) aligned concordantly >1 times ---- 1774063 pairs aligned concordantly 0 times; of these: 723036 (40.76%) aligned discordantly 1 time ---- 1051027 pairs aligned 0 times concordantly or discordantly; of these: 2102054 mates make up the pairs; of these: 838753 (39.90%) aligned 0 times 737922 (35.10%) aligned exactly 1 time 525379 (24.99%) aligned >1 times 99.12% overall alignment rate Time searching: 01:11:03 Overall time: 01:11:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 2120501 / 46052043 = 0.0460 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 04 Sep 2018 10:21:36: # Command line: callpeak -t SRX4012473.bam -f BAM -g ce -n SRX4012473.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4012473.05 # format = BAM # ChIP-seq file = ['SRX4012473.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 04 Sep 2018 10:21:36: # Command line: callpeak -t SRX4012473.bam -f BAM -g ce -n SRX4012473.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4012473.20 # format = BAM # ChIP-seq file = ['SRX4012473.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 04 Sep 2018 10:21:36: # Command line: callpeak -t SRX4012473.bam -f BAM -g ce -n SRX4012473.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4012473.10 # format = BAM # ChIP-seq file = ['SRX4012473.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 04 Sep 2018 10:21:36: #1 read tag files... INFO @ Tue, 04 Sep 2018 10:21:36: #1 read tag files... INFO @ Tue, 04 Sep 2018 10:21:36: #1 read tag files... INFO @ Tue, 04 Sep 2018 10:21:36: #1 read treatment tags... INFO @ Tue, 04 Sep 2018 10:21:36: #1 read treatment tags... INFO @ Tue, 04 Sep 2018 10:21:36: #1 read treatment tags... INFO @ Tue, 04 Sep 2018 10:21:42: 1000000 INFO @ Tue, 04 Sep 2018 10:21:42: 1000000 INFO @ Tue, 04 Sep 2018 10:21:42: 1000000 INFO @ Tue, 04 Sep 2018 10:21:47: 2000000 INFO @ Tue, 04 Sep 2018 10:21:48: 2000000 INFO @ Tue, 04 Sep 2018 10:21:48: 2000000 INFO @ Tue, 04 Sep 2018 10:21:53: 3000000 INFO @ Tue, 04 Sep 2018 10:21:53: 3000000 INFO @ Tue, 04 Sep 2018 10:21:53: 3000000 INFO @ Tue, 04 Sep 2018 10:21:59: 4000000 INFO @ Tue, 04 Sep 2018 10:21:59: 4000000 INFO @ Tue, 04 Sep 2018 10:21:59: 4000000 INFO @ Tue, 04 Sep 2018 10:22:04: 5000000 INFO @ Tue, 04 Sep 2018 10:22:05: 5000000 INFO @ Tue, 04 Sep 2018 10:22:05: 5000000 INFO @ Tue, 04 Sep 2018 10:22:10: 6000000 INFO @ Tue, 04 Sep 2018 10:22:11: 6000000 INFO @ Tue, 04 Sep 2018 10:22:11: 6000000 INFO @ Tue, 04 Sep 2018 10:22:16: 7000000 INFO @ Tue, 04 Sep 2018 10:22:17: 7000000 INFO @ Tue, 04 Sep 2018 10:22:17: 7000000 INFO @ Tue, 04 Sep 2018 10:22:21: 8000000 INFO @ Tue, 04 Sep 2018 10:22:23: 8000000 INFO @ Tue, 04 Sep 2018 10:22:23: 8000000 INFO @ Tue, 04 Sep 2018 10:22:27: 9000000 INFO @ Tue, 04 Sep 2018 10:22:29: 9000000 INFO @ Tue, 04 Sep 2018 10:22:29: 9000000 INFO @ Tue, 04 Sep 2018 10:22:33: 10000000 INFO @ Tue, 04 Sep 2018 10:22:35: 10000000 INFO @ Tue, 04 Sep 2018 10:22:35: 10000000 INFO @ Tue, 04 Sep 2018 10:22:38: 11000000 INFO @ Tue, 04 Sep 2018 10:22:41: 11000000 INFO @ Tue, 04 Sep 2018 10:22:41: 11000000 INFO @ Tue, 04 Sep 2018 10:22:44: 12000000 INFO @ Tue, 04 Sep 2018 10:22:47: 12000000 INFO @ Tue, 04 Sep 2018 10:22:47: 12000000 INFO @ Tue, 04 Sep 2018 10:22:49: 13000000 INFO @ Tue, 04 Sep 2018 10:22:53: 13000000 INFO @ Tue, 04 Sep 2018 10:22:53: 13000000 INFO @ Tue, 04 Sep 2018 10:22:55: 14000000 INFO @ Tue, 04 Sep 2018 10:22:59: 14000000 INFO @ Tue, 04 Sep 2018 10:23:00: 14000000 INFO @ Tue, 04 Sep 2018 10:23:01: 15000000 INFO @ Tue, 04 Sep 2018 10:23:05: 15000000 INFO @ Tue, 04 Sep 2018 10:23:06: 15000000 INFO @ Tue, 04 Sep 2018 10:23:06: 16000000 INFO @ Tue, 04 Sep 2018 10:23:11: 16000000 INFO @ Tue, 04 Sep 2018 10:23:12: 16000000 INFO @ Tue, 04 Sep 2018 10:23:12: 17000000 INFO @ Tue, 04 Sep 2018 10:23:17: 17000000 INFO @ Tue, 04 Sep 2018 10:23:18: 18000000 INFO @ Tue, 04 Sep 2018 10:23:18: 17000000 INFO @ Tue, 04 Sep 2018 10:23:23: 18000000 INFO @ Tue, 04 Sep 2018 10:23:23: 19000000 INFO @ Tue, 04 Sep 2018 10:23:24: 18000000 INFO @ Tue, 04 Sep 2018 10:23:29: 19000000 INFO @ Tue, 04 Sep 2018 10:23:29: 20000000 INFO @ Tue, 04 Sep 2018 10:23:30: 19000000 INFO @ Tue, 04 Sep 2018 10:23:34: 21000000 INFO @ Tue, 04 Sep 2018 10:23:35: 20000000 INFO @ Tue, 04 Sep 2018 10:23:36: 20000000 INFO @ Tue, 04 Sep 2018 10:23:40: 22000000 INFO @ Tue, 04 Sep 2018 10:23:41: 21000000 INFO @ Tue, 04 Sep 2018 10:23:42: 21000000 INFO @ Tue, 04 Sep 2018 10:23:46: 23000000 INFO @ Tue, 04 Sep 2018 10:23:47: 22000000 INFO @ Tue, 04 Sep 2018 10:23:48: 22000000 INFO @ Tue, 04 Sep 2018 10:23:51: 24000000 INFO @ Tue, 04 Sep 2018 10:23:53: 23000000 INFO @ Tue, 04 Sep 2018 10:23:54: 23000000 INFO @ Tue, 04 Sep 2018 10:23:57: 25000000 INFO @ Tue, 04 Sep 2018 10:23:59: 24000000 INFO @ Tue, 04 Sep 2018 10:24:00: 24000000 INFO @ Tue, 04 Sep 2018 10:24:03: 26000000 INFO @ Tue, 04 Sep 2018 10:24:05: 25000000 INFO @ Tue, 04 Sep 2018 10:24:07: 25000000 INFO @ Tue, 04 Sep 2018 10:24:08: 27000000 INFO @ Tue, 04 Sep 2018 10:24:11: 26000000 INFO @ Tue, 04 Sep 2018 10:24:13: 26000000 INFO @ Tue, 04 Sep 2018 10:24:14: 28000000 INFO @ Tue, 04 Sep 2018 10:24:17: 27000000 INFO @ Tue, 04 Sep 2018 10:24:19: 27000000 INFO @ Tue, 04 Sep 2018 10:24:20: 29000000 INFO @ Tue, 04 Sep 2018 10:24:23: 28000000 INFO @ Tue, 04 Sep 2018 10:24:25: 28000000 INFO @ Tue, 04 Sep 2018 10:24:25: 30000000 INFO @ Tue, 04 Sep 2018 10:24:29: 29000000 INFO @ Tue, 04 Sep 2018 10:24:31: 29000000 INFO @ Tue, 04 Sep 2018 10:24:31: 31000000 INFO @ Tue, 04 Sep 2018 10:24:35: 30000000 INFO @ Tue, 04 Sep 2018 10:24:36: 32000000 INFO @ Tue, 04 Sep 2018 10:24:37: 30000000 INFO @ Tue, 04 Sep 2018 10:24:41: 31000000 INFO @ Tue, 04 Sep 2018 10:24:42: 33000000 INFO @ Tue, 04 Sep 2018 10:24:43: 31000000 INFO @ Tue, 04 Sep 2018 10:24:47: 32000000 INFO @ Tue, 04 Sep 2018 10:24:48: 34000000 INFO @ Tue, 04 Sep 2018 10:24:49: 32000000 INFO @ Tue, 04 Sep 2018 10:24:53: 33000000 INFO @ Tue, 04 Sep 2018 10:24:53: 35000000 INFO @ Tue, 04 Sep 2018 10:24:55: 33000000 INFO @ Tue, 04 Sep 2018 10:24:59: 34000000 INFO @ Tue, 04 Sep 2018 10:24:59: 36000000 INFO @ Tue, 04 Sep 2018 10:25:01: 34000000 INFO @ Tue, 04 Sep 2018 10:25:05: 35000000 INFO @ Tue, 04 Sep 2018 10:25:05: 37000000 INFO @ Tue, 04 Sep 2018 10:25:07: 35000000 INFO @ Tue, 04 Sep 2018 10:25:10: 38000000 INFO @ Tue, 04 Sep 2018 10:25:10: 36000000 INFO @ Tue, 04 Sep 2018 10:25:13: 36000000 INFO @ Tue, 04 Sep 2018 10:25:16: 39000000 INFO @ Tue, 04 Sep 2018 10:25:16: 37000000 INFO @ Tue, 04 Sep 2018 10:25:19: 37000000 INFO @ Tue, 04 Sep 2018 10:25:22: 40000000 INFO @ Tue, 04 Sep 2018 10:25:22: 38000000 INFO @ Tue, 04 Sep 2018 10:25:25: 38000000 INFO @ Tue, 04 Sep 2018 10:25:27: 41000000 INFO @ Tue, 04 Sep 2018 10:25:29: 39000000 INFO @ Tue, 04 Sep 2018 10:25:31: 39000000 INFO @ Tue, 04 Sep 2018 10:25:33: 42000000 INFO @ Tue, 04 Sep 2018 10:25:35: 40000000 INFO @ Tue, 04 Sep 2018 10:25:37: 40000000 INFO @ Tue, 04 Sep 2018 10:25:39: 43000000 INFO @ Tue, 04 Sep 2018 10:25:41: 41000000 INFO @ Tue, 04 Sep 2018 10:25:43: 41000000 INFO @ Tue, 04 Sep 2018 10:25:44: 44000000 INFO @ Tue, 04 Sep 2018 10:25:47: 42000000 INFO @ Tue, 04 Sep 2018 10:25:50: 42000000 INFO @ Tue, 04 Sep 2018 10:25:50: 45000000 INFO @ Tue, 04 Sep 2018 10:25:53: 43000000 INFO @ Tue, 04 Sep 2018 10:25:55: 46000000 INFO @ Tue, 04 Sep 2018 10:25:56: 43000000 INFO @ Tue, 04 Sep 2018 10:25:59: 44000000 INFO @ Tue, 04 Sep 2018 10:26:01: 47000000 INFO @ Tue, 04 Sep 2018 10:26:02: 44000000 INFO @ Tue, 04 Sep 2018 10:26:05: 45000000 INFO @ Tue, 04 Sep 2018 10:26:07: 48000000 INFO @ Tue, 04 Sep 2018 10:26:08: 45000000 INFO @ Tue, 04 Sep 2018 10:26:11: 46000000 INFO @ Tue, 04 Sep 2018 10:26:12: 49000000 INFO @ Tue, 04 Sep 2018 10:26:14: 46000000 INFO @ Tue, 04 Sep 2018 10:26:17: 47000000 INFO @ Tue, 04 Sep 2018 10:26:18: 50000000 INFO @ Tue, 04 Sep 2018 10:26:20: 47000000 INFO @ Tue, 04 Sep 2018 10:26:23: 48000000 INFO @ Tue, 04 Sep 2018 10:26:24: 51000000 INFO @ Tue, 04 Sep 2018 10:26:26: 48000000 INFO @ Tue, 04 Sep 2018 10:26:29: 49000000 INFO @ Tue, 04 Sep 2018 10:26:29: 52000000 INFO @ Tue, 04 Sep 2018 10:26:32: 49000000 INFO @ Tue, 04 Sep 2018 10:26:35: 53000000 INFO @ Tue, 04 Sep 2018 10:26:35: 50000000 INFO @ Tue, 04 Sep 2018 10:26:38: 50000000 INFO @ Tue, 04 Sep 2018 10:26:40: 54000000 INFO @ Tue, 04 Sep 2018 10:26:41: 51000000 INFO @ Tue, 04 Sep 2018 10:26:44: 51000000 INFO @ Tue, 04 Sep 2018 10:26:46: 55000000 INFO @ Tue, 04 Sep 2018 10:26:47: 52000000 INFO @ Tue, 04 Sep 2018 10:26:50: 52000000 INFO @ Tue, 04 Sep 2018 10:26:52: 56000000 INFO @ Tue, 04 Sep 2018 10:26:53: 53000000 INFO @ Tue, 04 Sep 2018 10:26:56: 53000000 INFO @ Tue, 04 Sep 2018 10:26:57: 57000000 INFO @ Tue, 04 Sep 2018 10:26:59: 54000000 INFO @ Tue, 04 Sep 2018 10:27:02: 54000000 INFO @ Tue, 04 Sep 2018 10:27:03: 58000000 INFO @ Tue, 04 Sep 2018 10:27:05: 55000000 INFO @ Tue, 04 Sep 2018 10:27:09: 55000000 INFO @ Tue, 04 Sep 2018 10:27:09: 59000000 INFO @ Tue, 04 Sep 2018 10:27:11: 56000000 INFO @ Tue, 04 Sep 2018 10:27:14: 60000000 INFO @ Tue, 04 Sep 2018 10:27:15: 56000000 INFO @ Tue, 04 Sep 2018 10:27:17: 57000000 INFO @ Tue, 04 Sep 2018 10:27:20: 61000000 INFO @ Tue, 04 Sep 2018 10:27:21: 57000000 INFO @ Tue, 04 Sep 2018 10:27:23: 58000000 INFO @ Tue, 04 Sep 2018 10:27:26: 62000000 INFO @ Tue, 04 Sep 2018 10:27:27: 58000000 INFO @ Tue, 04 Sep 2018 10:27:29: 59000000 INFO @ Tue, 04 Sep 2018 10:27:31: 63000000 INFO @ Tue, 04 Sep 2018 10:27:34: 59000000 INFO @ Tue, 04 Sep 2018 10:27:35: 60000000 INFO @ Tue, 04 Sep 2018 10:27:37: 64000000 INFO @ Tue, 04 Sep 2018 10:27:40: 60000000 INFO @ Tue, 04 Sep 2018 10:27:41: 61000000 INFO @ Tue, 04 Sep 2018 10:27:42: 65000000 INFO @ Tue, 04 Sep 2018 10:27:46: 61000000 INFO @ Tue, 04 Sep 2018 10:27:47: 62000000 INFO @ Tue, 04 Sep 2018 10:27:48: 66000000 INFO @ Tue, 04 Sep 2018 10:27:52: 62000000 INFO @ Tue, 04 Sep 2018 10:27:53: 63000000 INFO @ Tue, 04 Sep 2018 10:27:54: 67000000 INFO @ Tue, 04 Sep 2018 10:27:58: 63000000 INFO @ Tue, 04 Sep 2018 10:27:59: 64000000 INFO @ Tue, 04 Sep 2018 10:27:59: 68000000 INFO @ Tue, 04 Sep 2018 10:28:04: 64000000 INFO @ Tue, 04 Sep 2018 10:28:05: 65000000 INFO @ Tue, 04 Sep 2018 10:28:05: 69000000 INFO @ Tue, 04 Sep 2018 10:28:10: 65000000 INFO @ Tue, 04 Sep 2018 10:28:10: 70000000 INFO @ Tue, 04 Sep 2018 10:28:11: 66000000 INFO @ Tue, 04 Sep 2018 10:28:16: 66000000 INFO @ Tue, 04 Sep 2018 10:28:16: 71000000 INFO @ Tue, 04 Sep 2018 10:28:17: 67000000 INFO @ Tue, 04 Sep 2018 10:28:22: 72000000 INFO @ Tue, 04 Sep 2018 10:28:22: 67000000 INFO @ Tue, 04 Sep 2018 10:28:22: 68000000 INFO @ Tue, 04 Sep 2018 10:28:27: 73000000 INFO @ Tue, 04 Sep 2018 10:28:28: 68000000 INFO @ Tue, 04 Sep 2018 10:28:28: 69000000 INFO @ Tue, 04 Sep 2018 10:28:33: 74000000 INFO @ Tue, 04 Sep 2018 10:28:34: 69000000 INFO @ Tue, 04 Sep 2018 10:28:34: 70000000 INFO @ Tue, 04 Sep 2018 10:28:39: 75000000 INFO @ Tue, 04 Sep 2018 10:28:40: 70000000 INFO @ Tue, 04 Sep 2018 10:28:41: 71000000 INFO @ Tue, 04 Sep 2018 10:28:44: 76000000 INFO @ Tue, 04 Sep 2018 10:28:46: 71000000 INFO @ Tue, 04 Sep 2018 10:28:47: 72000000 INFO @ Tue, 04 Sep 2018 10:28:50: 77000000 INFO @ Tue, 04 Sep 2018 10:28:52: 72000000 INFO @ Tue, 04 Sep 2018 10:28:53: 73000000 INFO @ Tue, 04 Sep 2018 10:28:56: 78000000 INFO @ Tue, 04 Sep 2018 10:28:58: 73000000 INFO @ Tue, 04 Sep 2018 10:28:59: 74000000 INFO @ Tue, 04 Sep 2018 10:29:01: 79000000 INFO @ Tue, 04 Sep 2018 10:29:05: 74000000 INFO @ Tue, 04 Sep 2018 10:29:05: 75000000 INFO @ Tue, 04 Sep 2018 10:29:07: 80000000 INFO @ Tue, 04 Sep 2018 10:29:11: 75000000 INFO @ Tue, 04 Sep 2018 10:29:11: 76000000 INFO @ Tue, 04 Sep 2018 10:29:13: 81000000 INFO @ Tue, 04 Sep 2018 10:29:17: 76000000 INFO @ Tue, 04 Sep 2018 10:29:17: 77000000 INFO @ Tue, 04 Sep 2018 10:29:18: 82000000 INFO @ Tue, 04 Sep 2018 10:29:23: 77000000 INFO @ Tue, 04 Sep 2018 10:29:23: 78000000 INFO @ Tue, 04 Sep 2018 10:29:24: 83000000 INFO @ Tue, 04 Sep 2018 10:29:29: 79000000 INFO @ Tue, 04 Sep 2018 10:29:29: 78000000 INFO @ Tue, 04 Sep 2018 10:29:30: 84000000 INFO @ Tue, 04 Sep 2018 10:29:35: 80000000 INFO @ Tue, 04 Sep 2018 10:29:35: 79000000 INFO @ Tue, 04 Sep 2018 10:29:35: 85000000 INFO @ Tue, 04 Sep 2018 10:29:41: 86000000 INFO @ Tue, 04 Sep 2018 10:29:41: 81000000 INFO @ Tue, 04 Sep 2018 10:29:41: 80000000 INFO @ Tue, 04 Sep 2018 10:29:46: 87000000 INFO @ Tue, 04 Sep 2018 10:29:47: 82000000 INFO @ Tue, 04 Sep 2018 10:29:47: 81000000 INFO @ Tue, 04 Sep 2018 10:29:52: 88000000 INFO @ Tue, 04 Sep 2018 10:29:53: 83000000 INFO @ Tue, 04 Sep 2018 10:29:53: 82000000 INFO @ Tue, 04 Sep 2018 10:29:58: 89000000 INFO @ Tue, 04 Sep 2018 10:29:59: 84000000 INFO @ Tue, 04 Sep 2018 10:29:59: 83000000 INFO @ Tue, 04 Sep 2018 10:30:03: 90000000 INFO @ Tue, 04 Sep 2018 10:30:05: 85000000 INFO @ Tue, 04 Sep 2018 10:30:05: #1 tag size is determined as 75 bps INFO @ Tue, 04 Sep 2018 10:30:05: #1 tag size = 75 INFO @ Tue, 04 Sep 2018 10:30:05: #1 total tags in treatment: 43797032 INFO @ Tue, 04 Sep 2018 10:30:05: #1 user defined the maximum tags... INFO @ Tue, 04 Sep 2018 10:30:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 04 Sep 2018 10:30:06: 84000000 INFO @ Tue, 04 Sep 2018 10:30:06: #1 tags after filtering in treatment: 37085467 INFO @ Tue, 04 Sep 2018 10:30:06: #1 Redundant rate of treatment: 0.15 INFO @ Tue, 04 Sep 2018 10:30:06: #1 finished! INFO @ Tue, 04 Sep 2018 10:30:06: #2 Build Peak Model... INFO @ Tue, 04 Sep 2018 10:30:06: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 04 Sep 2018 10:30:09: #2 number of paired peaks: 0 WARNING @ Tue, 04 Sep 2018 10:30:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 04 Sep 2018 10:30:09: Process for pairing-model is terminated! cat: SRX4012473.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 14 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4012473.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4012473.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4012473.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 04 Sep 2018 10:30:11: 86000000 INFO @ Tue, 04 Sep 2018 10:30:12: 85000000 INFO @ Tue, 04 Sep 2018 10:30:16: 87000000 INFO @ Tue, 04 Sep 2018 10:30:18: 86000000 INFO @ Tue, 04 Sep 2018 10:30:22: 88000000 INFO @ Tue, 04 Sep 2018 10:30:23: 87000000 INFO @ Tue, 04 Sep 2018 10:30:28: 89000000 INFO @ Tue, 04 Sep 2018 10:30:29: 88000000 INFO @ Tue, 04 Sep 2018 10:30:33: 90000000 INFO @ Tue, 04 Sep 2018 10:30:35: 89000000 INFO @ Tue, 04 Sep 2018 10:30:35: #1 tag size is determined as 75 bps INFO @ Tue, 04 Sep 2018 10:30:35: #1 tag size = 75 INFO @ Tue, 04 Sep 2018 10:30:35: #1 total tags in treatment: 43797032 INFO @ Tue, 04 Sep 2018 10:30:35: #1 user defined the maximum tags... INFO @ Tue, 04 Sep 2018 10:30:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 04 Sep 2018 10:30:36: #1 tags after filtering in treatment: 37085467 INFO @ Tue, 04 Sep 2018 10:30:36: #1 Redundant rate of treatment: 0.15 INFO @ Tue, 04 Sep 2018 10:30:36: #1 finished! INFO @ Tue, 04 Sep 2018 10:30:36: #2 Build Peak Model... INFO @ Tue, 04 Sep 2018 10:30:36: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 04 Sep 2018 10:30:39: #2 number of paired peaks: 0 WARNING @ Tue, 04 Sep 2018 10:30:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 04 Sep 2018 10:30:39: Process for pairing-model is terminated! cat: SRX4012473.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 4 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4012473.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4012473.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4012473.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 04 Sep 2018 10:30:40: 90000000 INFO @ Tue, 04 Sep 2018 10:30:42: #1 tag size is determined as 75 bps INFO @ Tue, 04 Sep 2018 10:30:42: #1 tag size = 75 INFO @ Tue, 04 Sep 2018 10:30:42: #1 total tags in treatment: 43797032 INFO @ Tue, 04 Sep 2018 10:30:42: #1 user defined the maximum tags... INFO @ Tue, 04 Sep 2018 10:30:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 04 Sep 2018 10:30:43: #1 tags after filtering in treatment: 37085467 INFO @ Tue, 04 Sep 2018 10:30:43: #1 Redundant rate of treatment: 0.15 INFO @ Tue, 04 Sep 2018 10:30:43: #1 finished! INFO @ Tue, 04 Sep 2018 10:30:43: #2 Build Peak Model... INFO @ Tue, 04 Sep 2018 10:30:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 04 Sep 2018 10:30:46: #2 number of paired peaks: 0 WARNING @ Tue, 04 Sep 2018 10:30:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 04 Sep 2018 10:30:46: Process for pairing-model is terminated! cat: SRX4012473.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4012473.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4012473.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4012473.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。