Job ID = 1292377 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 91,140,648 reads read : 182,281,296 reads written : 91,140,648 reads 0-length : 91,140,648 spots read : 77,772,415 reads read : 155,544,830 reads written : 77,772,415 reads 0-length : 77,772,415 rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:26:05 168913063 reads; of these: 168913063 (100.00%) were unpaired; of these: 95037273 (56.26%) aligned 0 times 59812394 (35.41%) aligned exactly 1 time 14063396 (8.33%) aligned >1 times 43.74% overall alignment rate Time searching: 00:26:05 Overall time: 00:26:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 32478748 / 73875790 = 0.4396 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 19:50:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3883436/SRX3883436.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3883436/SRX3883436.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3883436/SRX3883436.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3883436/SRX3883436.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 19:50:30: #1 read tag files... INFO @ Sun, 02 Jun 2019 19:50:30: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 19:50:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3883436/SRX3883436.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3883436/SRX3883436.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3883436/SRX3883436.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3883436/SRX3883436.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 19:50:30: #1 read tag files... INFO @ Sun, 02 Jun 2019 19:50:30: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 19:50:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3883436/SRX3883436.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3883436/SRX3883436.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3883436/SRX3883436.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3883436/SRX3883436.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 19:50:30: #1 read tag files... INFO @ Sun, 02 Jun 2019 19:50:30: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 19:50:38: 1000000 INFO @ Sun, 02 Jun 2019 19:50:38: 1000000 INFO @ Sun, 02 Jun 2019 19:50:39: 1000000 INFO @ Sun, 02 Jun 2019 19:50:46: 2000000 INFO @ Sun, 02 Jun 2019 19:50:46: 2000000 INFO @ Sun, 02 Jun 2019 19:50:47: 2000000 INFO @ Sun, 02 Jun 2019 19:50:52: 3000000 INFO @ Sun, 02 Jun 2019 19:50:54: 3000000 INFO @ Sun, 02 Jun 2019 19:50:56: 3000000 INFO @ Sun, 02 Jun 2019 19:50:59: 4000000 INFO @ Sun, 02 Jun 2019 19:51:01: 4000000 INFO @ Sun, 02 Jun 2019 19:51:04: 4000000 INFO @ Sun, 02 Jun 2019 19:51:06: 5000000 INFO @ Sun, 02 Jun 2019 19:51:09: 5000000 INFO @ Sun, 02 Jun 2019 19:51:13: 6000000 INFO @ Sun, 02 Jun 2019 19:51:13: 5000000 INFO @ Sun, 02 Jun 2019 19:51:16: 6000000 INFO @ Sun, 02 Jun 2019 19:51:19: 7000000 INFO @ Sun, 02 Jun 2019 19:51:21: 6000000 INFO @ Sun, 02 Jun 2019 19:51:23: 7000000 INFO @ Sun, 02 Jun 2019 19:51:26: 8000000 INFO @ Sun, 02 Jun 2019 19:51:29: 7000000 INFO @ Sun, 02 Jun 2019 19:51:30: 8000000 INFO @ Sun, 02 Jun 2019 19:51:32: 9000000 INFO @ Sun, 02 Jun 2019 19:51:37: 9000000 INFO @ Sun, 02 Jun 2019 19:51:38: 8000000 INFO @ Sun, 02 Jun 2019 19:51:39: 10000000 INFO @ Sun, 02 Jun 2019 19:51:45: 10000000 INFO @ Sun, 02 Jun 2019 19:51:45: 11000000 INFO @ Sun, 02 Jun 2019 19:51:46: 9000000 INFO @ Sun, 02 Jun 2019 19:51:52: 11000000 INFO @ Sun, 02 Jun 2019 19:51:52: 12000000 INFO @ Sun, 02 Jun 2019 19:51:54: 10000000 INFO @ Sun, 02 Jun 2019 19:51:59: 13000000 INFO @ Sun, 02 Jun 2019 19:51:59: 12000000 INFO @ Sun, 02 Jun 2019 19:52:02: 11000000 INFO @ Sun, 02 Jun 2019 19:52:06: 14000000 INFO @ Sun, 02 Jun 2019 19:52:06: 13000000 INFO @ Sun, 02 Jun 2019 19:52:11: 12000000 INFO @ Sun, 02 Jun 2019 19:52:13: 15000000 INFO @ Sun, 02 Jun 2019 19:52:14: 14000000 INFO @ Sun, 02 Jun 2019 19:52:19: 13000000 INFO @ Sun, 02 Jun 2019 19:52:19: 16000000 INFO @ Sun, 02 Jun 2019 19:52:21: 15000000 INFO @ Sun, 02 Jun 2019 19:52:26: 17000000 INFO @ Sun, 02 Jun 2019 19:52:27: 14000000 INFO @ Sun, 02 Jun 2019 19:52:28: 16000000 INFO @ Sun, 02 Jun 2019 19:52:33: 18000000 INFO @ Sun, 02 Jun 2019 19:52:36: 15000000 INFO @ Sun, 02 Jun 2019 19:52:37: 17000000 INFO @ Sun, 02 Jun 2019 19:52:41: 19000000 INFO @ Sun, 02 Jun 2019 19:52:45: 16000000 INFO @ Sun, 02 Jun 2019 19:52:46: 18000000 INFO @ Sun, 02 Jun 2019 19:52:48: 20000000 INFO @ Sun, 02 Jun 2019 19:52:54: 19000000 INFO @ Sun, 02 Jun 2019 19:52:54: 17000000 INFO @ Sun, 02 Jun 2019 19:52:56: 21000000 INFO @ Sun, 02 Jun 2019 19:53:01: 20000000 INFO @ Sun, 02 Jun 2019 19:53:03: 18000000 INFO @ Sun, 02 Jun 2019 19:53:04: 22000000 INFO @ Sun, 02 Jun 2019 19:53:09: 21000000 INFO @ Sun, 02 Jun 2019 19:53:11: 19000000 INFO @ Sun, 02 Jun 2019 19:53:11: 23000000 INFO @ Sun, 02 Jun 2019 19:53:16: 22000000 INFO @ Sun, 02 Jun 2019 19:53:19: 24000000 INFO @ Sun, 02 Jun 2019 19:53:20: 20000000 INFO @ Sun, 02 Jun 2019 19:53:24: 23000000 INFO @ Sun, 02 Jun 2019 19:53:27: 25000000 INFO @ Sun, 02 Jun 2019 19:53:28: 21000000 INFO @ Sun, 02 Jun 2019 19:53:31: 24000000 INFO @ Sun, 02 Jun 2019 19:53:34: 26000000 INFO @ Sun, 02 Jun 2019 19:53:37: 22000000 INFO @ Sun, 02 Jun 2019 19:53:38: 25000000 INFO @ Sun, 02 Jun 2019 19:53:42: 27000000 INFO @ Sun, 02 Jun 2019 19:53:45: 23000000 INFO @ Sun, 02 Jun 2019 19:53:46: 26000000 INFO @ Sun, 02 Jun 2019 19:53:49: 28000000 INFO @ Sun, 02 Jun 2019 19:53:53: 27000000 INFO @ Sun, 02 Jun 2019 19:53:54: 24000000 INFO @ Sun, 02 Jun 2019 19:53:57: 29000000 INFO @ Sun, 02 Jun 2019 19:54:01: 28000000 INFO @ Sun, 02 Jun 2019 19:54:02: 25000000 INFO @ Sun, 02 Jun 2019 19:54:04: 30000000 INFO @ Sun, 02 Jun 2019 19:54:08: 29000000 INFO @ Sun, 02 Jun 2019 19:54:10: 26000000 INFO @ Sun, 02 Jun 2019 19:54:11: 31000000 INFO @ Sun, 02 Jun 2019 19:54:15: 30000000 INFO @ Sun, 02 Jun 2019 19:54:17: 32000000 INFO @ Sun, 02 Jun 2019 19:54:18: 27000000 INFO @ Sun, 02 Jun 2019 19:54:22: 31000000 INFO @ Sun, 02 Jun 2019 19:54:24: 33000000 INFO @ Sun, 02 Jun 2019 19:54:26: 28000000 INFO @ Sun, 02 Jun 2019 19:54:29: 32000000 INFO @ Sun, 02 Jun 2019 19:54:30: 34000000 INFO @ Sun, 02 Jun 2019 19:54:34: 29000000 INFO @ Sun, 02 Jun 2019 19:54:36: 33000000 INFO @ Sun, 02 Jun 2019 19:54:37: 35000000 INFO @ Sun, 02 Jun 2019 19:54:43: 30000000 INFO @ Sun, 02 Jun 2019 19:54:43: 34000000 INFO @ Sun, 02 Jun 2019 19:54:44: 36000000 INFO @ Sun, 02 Jun 2019 19:54:50: 35000000 INFO @ Sun, 02 Jun 2019 19:54:50: 37000000 INFO @ Sun, 02 Jun 2019 19:54:51: 31000000 INFO @ Sun, 02 Jun 2019 19:54:57: 38000000 INFO @ Sun, 02 Jun 2019 19:54:57: 36000000 INFO @ Sun, 02 Jun 2019 19:54:59: 32000000 INFO @ Sun, 02 Jun 2019 19:55:04: 39000000 INFO @ Sun, 02 Jun 2019 19:55:04: 37000000 INFO @ Sun, 02 Jun 2019 19:55:07: 33000000 INFO @ Sun, 02 Jun 2019 19:55:10: 40000000 INFO @ Sun, 02 Jun 2019 19:55:11: 38000000 INFO @ Sun, 02 Jun 2019 19:55:15: 34000000 INFO @ Sun, 02 Jun 2019 19:55:17: 41000000 INFO @ Sun, 02 Jun 2019 19:55:19: 39000000 INFO @ Sun, 02 Jun 2019 19:55:20: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 19:55:20: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 19:55:20: #1 total tags in treatment: 41397042 INFO @ Sun, 02 Jun 2019 19:55:20: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 19:55:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 19:55:21: #1 tags after filtering in treatment: 41397042 INFO @ Sun, 02 Jun 2019 19:55:21: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 19:55:21: #1 finished! INFO @ Sun, 02 Jun 2019 19:55:21: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 19:55:21: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 19:55:23: 35000000 INFO @ Sun, 02 Jun 2019 19:55:24: #2 number of paired peaks: 0 WARNING @ Sun, 02 Jun 2019 19:55:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 19:55:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3883436/SRX3883436.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3883436/SRX3883436.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3883436/SRX3883436.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3883436/SRX3883436.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 19:55:26: 40000000 INFO @ Sun, 02 Jun 2019 19:55:30: 36000000 INFO @ Sun, 02 Jun 2019 19:55:33: 41000000 INFO @ Sun, 02 Jun 2019 19:55:37: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 19:55:37: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 19:55:37: #1 total tags in treatment: 41397042 INFO @ Sun, 02 Jun 2019 19:55:37: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 19:55:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 19:55:38: #1 tags after filtering in treatment: 41397042 INFO @ Sun, 02 Jun 2019 19:55:38: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 19:55:38: #1 finished! INFO @ Sun, 02 Jun 2019 19:55:38: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 19:55:38: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 19:55:38: 37000000 INFO @ Sun, 02 Jun 2019 19:55:41: #2 number of paired peaks: 0 WARNING @ Sun, 02 Jun 2019 19:55:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 19:55:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3883436/SRX3883436.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3883436/SRX3883436.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3883436/SRX3883436.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3883436/SRX3883436.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 19:55:47: 38000000 INFO @ Sun, 02 Jun 2019 19:55:55: 39000000 INFO @ Sun, 02 Jun 2019 19:56:04: 40000000 INFO @ Sun, 02 Jun 2019 19:56:13: 41000000 INFO @ Sun, 02 Jun 2019 19:56:16: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 19:56:16: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 19:56:16: #1 total tags in treatment: 41397042 INFO @ Sun, 02 Jun 2019 19:56:16: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 19:56:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 19:56:17: #1 tags after filtering in treatment: 41397042 INFO @ Sun, 02 Jun 2019 19:56:17: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 19:56:17: #1 finished! INFO @ Sun, 02 Jun 2019 19:56:17: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 19:56:17: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 19:56:21: #2 number of paired peaks: 0 WARNING @ Sun, 02 Jun 2019 19:56:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 19:56:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3883436/SRX3883436.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3883436/SRX3883436.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3883436/SRX3883436.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3883436/SRX3883436.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。