Job ID = 11170851 sra ファイルのダウンロード中... Completed: 959814K bytes transferred in 96 seconds (81142K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 33663924 spots for /home/okishinya/chipatlas/results/ce10/SRX3583351/SRR6494007.sra Written 33663924 spots for /home/okishinya/chipatlas/results/ce10/SRX3583351/SRR6494007.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:03 33663924 reads; of these: 33663924 (100.00%) were unpaired; of these: 558083 (1.66%) aligned 0 times 27552419 (81.85%) aligned exactly 1 time 5553422 (16.50%) aligned >1 times 98.34% overall alignment rate Time searching: 00:14:03 Overall time: 00:14:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4710518 / 33105841 = 0.1423 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 08 Sep 2018 11:51:33: # Command line: callpeak -t SRX3583351.bam -f BAM -g ce -n SRX3583351.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3583351.10 # format = BAM # ChIP-seq file = ['SRX3583351.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 11:51:33: #1 read tag files... INFO @ Sat, 08 Sep 2018 11:51:33: # Command line: callpeak -t SRX3583351.bam -f BAM -g ce -n SRX3583351.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3583351.05 # format = BAM # ChIP-seq file = ['SRX3583351.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 11:51:33: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 11:51:33: #1 read tag files... INFO @ Sat, 08 Sep 2018 11:51:33: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 11:51:33: # Command line: callpeak -t SRX3583351.bam -f BAM -g ce -n SRX3583351.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3583351.20 # format = BAM # ChIP-seq file = ['SRX3583351.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 11:51:33: #1 read tag files... INFO @ Sat, 08 Sep 2018 11:51:33: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 11:51:40: 1000000 INFO @ Sat, 08 Sep 2018 11:51:40: 1000000 INFO @ Sat, 08 Sep 2018 11:51:40: 1000000 INFO @ Sat, 08 Sep 2018 11:51:46: 2000000 INFO @ Sat, 08 Sep 2018 11:51:46: 2000000 INFO @ Sat, 08 Sep 2018 11:51:48: 2000000 INFO @ Sat, 08 Sep 2018 11:51:53: 3000000 INFO @ Sat, 08 Sep 2018 11:51:53: 3000000 INFO @ Sat, 08 Sep 2018 11:51:55: 3000000 INFO @ Sat, 08 Sep 2018 11:52:01: 4000000 INFO @ Sat, 08 Sep 2018 11:52:02: 4000000 INFO @ Sat, 08 Sep 2018 11:52:03: 4000000 INFO @ Sat, 08 Sep 2018 11:52:09: 5000000 INFO @ Sat, 08 Sep 2018 11:52:10: 5000000 INFO @ Sat, 08 Sep 2018 11:52:11: 5000000 INFO @ Sat, 08 Sep 2018 11:52:19: 6000000 INFO @ Sat, 08 Sep 2018 11:52:20: 6000000 INFO @ Sat, 08 Sep 2018 11:52:20: 6000000 INFO @ Sat, 08 Sep 2018 11:52:28: 7000000 INFO @ Sat, 08 Sep 2018 11:52:29: 7000000 INFO @ Sat, 08 Sep 2018 11:52:30: 7000000 INFO @ Sat, 08 Sep 2018 11:52:37: 8000000 INFO @ Sat, 08 Sep 2018 11:52:37: 8000000 INFO @ Sat, 08 Sep 2018 11:52:38: 8000000 INFO @ Sat, 08 Sep 2018 11:52:45: 9000000 INFO @ Sat, 08 Sep 2018 11:52:45: 9000000 INFO @ Sat, 08 Sep 2018 11:52:47: 9000000 INFO @ Sat, 08 Sep 2018 11:52:54: 10000000 INFO @ Sat, 08 Sep 2018 11:52:54: 10000000 INFO @ Sat, 08 Sep 2018 11:52:56: 10000000 INFO @ Sat, 08 Sep 2018 11:53:03: 11000000 INFO @ Sat, 08 Sep 2018 11:53:03: 11000000 INFO @ Sat, 08 Sep 2018 11:53:05: 11000000 INFO @ Sat, 08 Sep 2018 11:53:13: 12000000 INFO @ Sat, 08 Sep 2018 11:53:13: 12000000 INFO @ Sat, 08 Sep 2018 11:53:14: 12000000 INFO @ Sat, 08 Sep 2018 11:53:22: 13000000 INFO @ Sat, 08 Sep 2018 11:53:23: 13000000 INFO @ Sat, 08 Sep 2018 11:53:23: 13000000 INFO @ Sat, 08 Sep 2018 11:53:30: 14000000 INFO @ Sat, 08 Sep 2018 11:53:31: 14000000 INFO @ Sat, 08 Sep 2018 11:53:33: 14000000 INFO @ Sat, 08 Sep 2018 11:53:39: 15000000 INFO @ Sat, 08 Sep 2018 11:53:40: 15000000 INFO @ Sat, 08 Sep 2018 11:53:42: 15000000 INFO @ Sat, 08 Sep 2018 11:53:47: 16000000 INFO @ Sat, 08 Sep 2018 11:53:47: 16000000 INFO @ Sat, 08 Sep 2018 11:53:52: 16000000 INFO @ Sat, 08 Sep 2018 11:53:54: 17000000 INFO @ Sat, 08 Sep 2018 11:53:55: 17000000 INFO @ Sat, 08 Sep 2018 11:54:01: 17000000 INFO @ Sat, 08 Sep 2018 11:54:02: 18000000 INFO @ Sat, 08 Sep 2018 11:54:02: 18000000 INFO @ Sat, 08 Sep 2018 11:54:09: 19000000 INFO @ Sat, 08 Sep 2018 11:54:09: 19000000 INFO @ Sat, 08 Sep 2018 11:54:11: 18000000 INFO @ Sat, 08 Sep 2018 11:54:16: 20000000 INFO @ Sat, 08 Sep 2018 11:54:16: 20000000 INFO @ Sat, 08 Sep 2018 11:54:19: 19000000 INFO @ Sat, 08 Sep 2018 11:54:23: 21000000 INFO @ Sat, 08 Sep 2018 11:54:24: 21000000 INFO @ Sat, 08 Sep 2018 11:54:28: 20000000 INFO @ Sat, 08 Sep 2018 11:54:32: 22000000 INFO @ Sat, 08 Sep 2018 11:54:32: 22000000 INFO @ Sat, 08 Sep 2018 11:54:36: 21000000 INFO @ Sat, 08 Sep 2018 11:54:40: 23000000 INFO @ Sat, 08 Sep 2018 11:54:40: 23000000 INFO @ Sat, 08 Sep 2018 11:54:44: 22000000 INFO @ Sat, 08 Sep 2018 11:54:48: 24000000 INFO @ Sat, 08 Sep 2018 11:54:48: 24000000 INFO @ Sat, 08 Sep 2018 11:54:52: 23000000 INFO @ Sat, 08 Sep 2018 11:54:56: 25000000 INFO @ Sat, 08 Sep 2018 11:54:57: 25000000 INFO @ Sat, 08 Sep 2018 11:55:01: 24000000 INFO @ Sat, 08 Sep 2018 11:55:04: 26000000 INFO @ Sat, 08 Sep 2018 11:55:05: 26000000 INFO @ Sat, 08 Sep 2018 11:55:09: 25000000 INFO @ Sat, 08 Sep 2018 11:55:13: 27000000 INFO @ Sat, 08 Sep 2018 11:55:13: 27000000 INFO @ Sat, 08 Sep 2018 11:55:17: 26000000 INFO @ Sat, 08 Sep 2018 11:55:21: 28000000 INFO @ Sat, 08 Sep 2018 11:55:22: 28000000 INFO @ Sat, 08 Sep 2018 11:55:25: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 11:55:25: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 11:55:25: #1 total tags in treatment: 28395323 INFO @ Sat, 08 Sep 2018 11:55:25: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 11:55:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 11:55:25: #1 tags after filtering in treatment: 28395323 INFO @ Sat, 08 Sep 2018 11:55:25: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 11:55:25: #1 finished! INFO @ Sat, 08 Sep 2018 11:55:25: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 11:55:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 11:55:26: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 11:55:26: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 11:55:26: #1 total tags in treatment: 28395323 INFO @ Sat, 08 Sep 2018 11:55:26: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 11:55:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 11:55:26: 27000000 INFO @ Sat, 08 Sep 2018 11:55:26: #1 tags after filtering in treatment: 28395323 INFO @ Sat, 08 Sep 2018 11:55:26: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 11:55:26: #1 finished! INFO @ Sat, 08 Sep 2018 11:55:26: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 11:55:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 11:55:27: #2 number of paired peaks: 74 WARNING @ Sat, 08 Sep 2018 11:55:27: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 11:55:27: Process for pairing-model is terminated! cat: SRX3583351.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 7 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3583351.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3583351.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3583351.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 11:55:28: #2 number of paired peaks: 74 WARNING @ Sat, 08 Sep 2018 11:55:28: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 11:55:28: Process for pairing-model is terminated! cat: SRX3583351.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3583351.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3583351.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3583351.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 11:55:33: 28000000 INFO @ Sat, 08 Sep 2018 11:55:37: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 11:55:37: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 11:55:37: #1 total tags in treatment: 28395323 INFO @ Sat, 08 Sep 2018 11:55:37: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 11:55:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 11:55:37: #1 tags after filtering in treatment: 28395323 INFO @ Sat, 08 Sep 2018 11:55:37: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 11:55:37: #1 finished! INFO @ Sat, 08 Sep 2018 11:55:37: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 11:55:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 11:55:39: #2 number of paired peaks: 74 WARNING @ Sat, 08 Sep 2018 11:55:39: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 11:55:39: Process for pairing-model is terminated! cat: SRX3583351.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3583351.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3583351.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3583351.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。